Restriction map for
pF1KF0006
(Circular) (Six-base) MAPSORT of: pF1KF0006.seq Check: 8241 from: 1 to: 5360
pF1KF0006 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 135 2091 135
Size: 1956 3404
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 2159 2376 2159
Size: 217 5143
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 1855 1941 2223 2314 2319 3185 3617 4364
Size: 1054 86 282 91 5 866 432 747
Cuts at: 4364 4632 801
Size: 268 1529
Fragments arranged by size:
1529 1054 866 747 432 282 268 91
86 5
AflII C'TTAA_G
Cuts at: 1810 1810
Size: 5360
AflIII A'CryG_T
Cuts at: 2712 3816 4793 2712
Size: 1104 977 3279
Fragments arranged by size:
3279 1104 977
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AleI CACnn'nnGTG
Cuts at: 474 1195 1633 474
Size: 721 438 4201
Fragments arranged by size:
4201 721 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 664 844 664
Size: 180 5180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1084 1116 1084
Size: 32 5328
AlwNI CAG_nnn'CTG
Cuts at: 330 882 1260 2106 2292 2665 4232 330
Size: 552 378 846 186 373 1567 1458
Fragments arranged by size:
1567 1458 846 552 378 373 186
ApaI G_GGCC'C
Cuts at: 498 678 498
Size: 180 5180
ApaLI G'TGCA_C
Cuts at: 1449 4130 1449
Size: 2681 2679
ApoI r'AATT_y
Cuts at: 2364 4713 4969 2364
Size: 2349 256 2755
Fragments arranged by size:
2755 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 1025 2187 2380 1025
Size: 1162 193 4005
Fragments arranged by size:
4005 1162 193
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 648 715 1270 1553 1858 2159 2376 2790 3038
Size: 67 555 283 305 301 217 414 248
Cuts at: 3038 3073 648
Size: 35 2935
Fragments arranged by size:
2935 555 414 305 301 283 248 217
67 35
BanII G_rGCy'C
Cuts at: 498 678 1024 1673 2093 2216 2264 2374 3404
Size: 180 346 649 420 123 48 110 1030
Cuts at: 3404 498
Size: 2454
Fragments arranged by size:
2454 1030 649 420 346 180 123 110
48
BbeI G_GCGC'C
Cuts at: 1557 1862 2794 3042 1557
Size: 305 932 248 3875
Fragments arranged by size:
3875 932 305 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1873 1907 2207 2241 1873
Size: 34 300 34 4992
Fragments arranged by size:
4992 300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3258 4019 5360 3258
Size: 761 1341 3258
Fragments arranged by size:
3258 1341 761
BclI T'GATC_A
Cuts at: 932 2880 932
Size: 1948 3412
BglI GCCn_nnn'nGGC
Cuts at: 1685 1813 1685
Size: 128 5232
BglII A'GATC_T
Cuts at: 176 2875 4912 176
Size: 2699 2037 624
Fragments arranged by size:
2699 2037 624
BlpI GC'TnA_GC
Cuts at: 2472 2472
Size: 5360
Bme1580I G_kGCm'C
Cuts at: 498 653 678 718 1453 2985 3078 4134 498
Size: 155 25 40 735 1532 93 1056 1724
Fragments arranged by size:
1724 1532 1056 735 155 93 40 25
BmrI ACTGGGnnnn_n'
Cuts at: 2265 2572 2653 2988 5132 2265
Size: 307 81 335 2144 2493
Fragments arranged by size:
2493 2144 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 297 1813 3727 297
Size: 1516 1914 1930
Fragments arranged by size:
1930 1914 1516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 370 772 889 1381 1469 2118 2140 267
Size: 103 402 117 492 88 649 22 3487
Fragments arranged by size:
3487 649 492 402 117 103 88 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1354 2536 3415 3907 4205 1048
Size: 306 1182 879 492 298 2203
Fragments arranged by size:
2203 1182 879 492 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 556 5064 556
Size: 4508 852
BsaAI yAC'GTr
Cuts at: 1530 2713 1530
Size: 1183 4177
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 764 1554 1859 2303 2791 3039 3741 5237 764
Size: 790 305 444 488 248 702 1496 887
Fragments arranged by size:
1496 887 790 702 488 444 305 248
BsaWI w'CCGG_w
Cuts at: 2549 3070 3748 4022 4169 4580 2549
Size: 521 678 274 147 411 3329
Fragments arranged by size:
3329 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1006 1036 1084 1114 1906 1936 2143 2173 1006
Size: 30 48 30 792 30 207 30 4193
Fragments arranged by size:
4193 792 207 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 772 1900 2008 769
Size: 3 1128 108 4121
Fragments arranged by size:
4121 1128 108 3
BseYI C'CCAG_C
Cuts at: 610 831 1047 1310 1602 1658 2192 2339 2785
Size: 221 216 263 292 56 534 147 446
Cuts at: 2785 3543 4120 4616 610
Size: 758 577 496 1354
Fragments arranged by size:
1354 758 577 534 496 446 292 263
221 216 147 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1042 2035 2092 925
Size: 117 993 57 4193
Fragments arranged by size:
4193 993 117 57
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4156 104
Size: 2798 46 1208 1308
Fragments arranged by size:
2798 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 671 1453 1540 1655 1673 1946 2129 2216 2264
Size: 782 87 115 18 273 183 87 48
Cuts at: 2264 2374 3152 3342 4134 671
Size: 110 778 190 792 1897
Fragments arranged by size:
1897 792 782 778 273 190 183 115
110 87 87 48 18
BsmBI CGTCTCn'nnnn_
Cuts at: 408 408
Size: 5360
Bsp1286I G_dGCh'C
Cuts at: 498 653 671 678 718 1024 1453 1540 1655
Size: 155 18 7 40 306 429 87 115
Cuts at: 1655 1673 1946 2093 2129 2216 2264 2374 2985
Size: 18 273 147 36 87 48 110 611
Cuts at: 2985 3078 3152 3342 3404 4134 498
Size: 93 74 190 62 730 1724
Fragments arranged by size:
1724 730 611 429 306 273 190 155
147 115 110 93 87 87 74 62
48 40 36 18 18 7
BspEI T'CCGG_A
Cuts at: 4580 4580
Size: 5360
BspHI T'CATG_A
Cuts at: 4536 4536
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 135 602 806 890 2091 2410 2624 2926 3307
Size: 467 204 84 1201 319 214 302 381
Cuts at: 3307 135
Size: 2188
Fragments arranged by size:
2188 1201 467 381 319 302 214 204
84
BsrBI CCG'CTC
Cuts at: 50 416 431 1584 2072 3652 3706 5190 5355
Size: 366 15 1153 488 1580 54 1484 165
Cuts at: 5355 50
Size: 55
Fragments arranged by size:
1580 1484 1153 488 366 165 55 54
15
BsrDI GCAATG_nn'
Cuts at: 1238 3272 1238
Size: 2034 3326
BsrFI r'CCGG_y
Cuts at: 445 718 1180 1267 1550 3358 3539 3748 445
Size: 273 462 87 283 1808 181 209 2057
Fragments arranged by size:
2057 1808 462 283 273 209 181 87
BsrGI T'GTAC_A
Cuts at: 254 254
Size: 5360
BssSI C'ACGA_G
Cuts at: 1519 1783 3631 3989 1519
Size: 264 1848 358 2890
Fragments arranged by size:
2890 1848 358 264
BstAPI GCAn_nnn'nTGC
Cuts at: 620 2047 2106 2737 620
Size: 1427 59 631 3243
Fragments arranged by size:
3243 1427 631 59
BstBI TT'CG_AA
Cuts at: 1823 3721 1823
Size: 1898 3462
BstXI CCAn_nnnn'nTGG
Cuts at: 291 476 831 972 1176 1707 291
Size: 185 355 141 204 531 3944
Fragments arranged by size:
3944 531 355 204 185 141
BstYI r'GATC_y
Cuts at: 1 176 378 2385 2852 2875 3210 3456 4468
Size: 175 202 2007 467 23 335 246 1012
Cuts at: 4468 4554 4566 4912 1
Size: 86 12 346 449
Fragments arranged by size:
2007 1012 467 449 346 335 246 202
175 86 23 12
BtgI C'CryG_G
Cuts at: 1202 2147 1202
Size: 945 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 552 1728 3310 3491 3654 552
Size: 1176 1582 181 163 2258
Fragments arranged by size:
2258 1582 1176 181 163
BtsI GCAGTG_nn'
Cuts at: 937 1331 1653 2048 2603 937
Size: 394 322 395 555 3694
Fragments arranged by size:
3694 555 395 394 322
DraI TTT'AAA
Cuts at: 2359 2359
Size: 5360
DraIII CAC_nnn'GTG
Cuts at: 372 372
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 853 3066 3924 853
Size: 2213 858 2289
Fragments arranged by size:
2289 2213 858
EaeI y'GGCC_r
Cuts at: 182 443 728 1166 1178 1199 2945 3119 3510
Size: 261 285 438 12 21 1746 174 391
Cuts at: 3510 3537 4882 182
Size: 27 1345 660
Fragments arranged by size:
1746 1345 660 438 391 285 261 174
27 21 12
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 197 502 1067 1348 2266 2338 3383 3593 197
Size: 305 565 281 918 72 1045 210 1964
Fragments arranged by size:
1964 1045 918 565 305 281 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 3878 4024 5170 3568
Size: 310 146 1146 3758
Fragments arranged by size:
3758 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1671 2214 2262 2372 1671
Size: 543 48 110 4659
Fragments arranged by size:
4659 543 110 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 370 772 801 889 1381 1469 1855 1941
Size: 103 402 29 88 492 88 386 86
Cuts at: 1941 2118 2140 2223 2314 2319 3185 3617 4364
Size: 177 22 83 91 5 866 432 747
Cuts at: 4364 4632 267
Size: 268 995
Fragments arranged by size:
995 866 747 492 432 402 386 268
177 103 91 88 88 86 83 29
22 5
EcoNI CCTnn'n_nnAGG
Cuts at: 754 1066 1288 2248 2296 754
Size: 312 222 960 48 3818
Fragments arranged by size:
3818 960 312 222 48
EcoO109I rG'GnC_Cy
Cuts at: 494 495 591 675 795 855 1002 1307 1566
Size: 1 96 84 120 60 147 305 259
Cuts at: 1566 1793 1815 2499 494
Size: 227 22 684 3355
Fragments arranged by size:
3355 684 305 259 227 147 120 96
84 60 22 1
EcoRI G'AATT_C
Cuts at: 2364 2364
Size: 5360
EcoRV GAT'ATC
Cuts at: 1125 1125
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5360
FspI TGC'GCA
Cuts at: 661 994 2182 3141 661
Size: 333 1188 959 2880
Fragments arranged by size:
2880 1188 959 333
HaeII r_GCGC'y
Cuts at: 1218 1557 1862 2794 3042 4064 5043 1218
Size: 339 305 932 248 1022 979 1535
Fragments arranged by size:
1535 1022 979 932 339 305 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 1906 1938 3202 3232 3234 3264 1114
Size: 32 760 32 1264 30 2 30 3210
Fragments arranged by size:
3210 1264 760 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2399 2399
Size: 5360
KasI G'GCGC_C
Cuts at: 1553 1858 2790 3038 1553
Size: 305 932 248 3875
Fragments arranged by size:
3875 932 305 248
KpnI G_GTAC'C
Cuts at: 2163 2380 2163
Size: 217 5143
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 1943 4031 4215 578
Size: 1365 2088 184 1723
Fragments arranged by size:
2088 1723 1365 184
MscI TGG'CCA
Cuts at: 184 1168 3121 184
Size: 984 1953 2423
Fragments arranged by size:
2423 1953 984
MslI CAynn'nnrTG
Cuts at: 474 646 721 1195 1633 2317 3349 3476 474
Size: 172 75 474 438 684 1032 127 2358
Fragments arranged by size:
2358 1032 684 474 438 172 127 75
MspA1I CmG'CkG
Cuts at: 474 852 1314 1512 2339 2471 2785 3145 4158
Size: 378 462 198 827 132 314 360 1013
Cuts at: 4158 4403 474
Size: 245 1431
Fragments arranged by size:
1431 1013 827 462 378 360 314 245
198 132
NaeI GCC'GGC
Cuts at: 447 1182 1269 3541 447
Size: 735 87 2272 2266
Fragments arranged by size:
2272 2266 735 87
NarI GG'CG_CC
Cuts at: 1554 1859 2791 3039 1554
Size: 305 932 248 3875
Fragments arranged by size:
3875 932 305 248
NdeI CA'TA_TG
Cuts at: 163 163
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 445 1180 1267 3539 445
Size: 735 87 2272 2266
Fragments arranged by size:
2272 2266 735 87
NspI r_CATG'y
Cuts at: 1056 2050 2316 2413 2913 3820 1056
Size: 994 266 97 500 907 2596
Fragments arranged by size:
2596 994 907 500 266 97
PciI A'CATG_T
Cuts at: 3816 3816
Size: 5360
PflMI CCAn_nnn'nTGG
Cuts at: 372 1260 372
Size: 888 4472
PfoI T'CCnGG_A
Cuts at: 749 905 1412 1715 2306 749
Size: 156 507 303 591 3803
Fragments arranged by size:
3803 591 507 303 156
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1084 1116 4499 4531 1084
Size: 32 3383 32 1913
Fragments arranged by size:
3383 1913 32 32
PpuMI rG'GwC_Cy
Cuts at: 591 795 855 1002 1307 1793 591
Size: 204 60 147 305 486 4158
Fragments arranged by size:
4158 486 305 204 147 60
PspOMI G'GGCC_C
Cuts at: 494 674 494
Size: 180 5180
PstI C_TGCA'G
Cuts at: 503 1471 1896 2407 3092 503
Size: 968 425 511 685 2771
Fragments arranged by size:
2771 968 685 511 425
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 474 2339 2785 3145 474
Size: 1865 446 360 2689
Fragments arranged by size:
2689 1865 446 360
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 1673 2216 2264 2374 1673
Size: 543 48 110 4659
Fragments arranged by size:
4659 543 110 48
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SanDI GG'GwC_CC
Cuts at: 1793 1793
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 1067 1348 3383 3593 1067
Size: 281 2035 210 2834
Fragments arranged by size:
2834 2035 281 210
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 1738 4877 1738
Size: 3139 2221
SfcI C'TryA_G
Cuts at: 33 499 1467 1491 1892 2403 3088 4081 4272
Size: 466 968 24 401 511 685 993 191
Cuts at: 4272 33
Size: 1121
Fragments arranged by size:
1121 993 968 685 511 466 401 191
24
SfiI GGCCn_nnn'nGGCC
Cuts at: 1813 1813
Size: 5360
SfoI GGC'GCC
Cuts at: 1555 1860 2792 3040 1555
Size: 305 932 248 3875
Fragments arranged by size:
3875 932 305 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SgrAI Cr'CCGG_yG
Cuts at: 1550 1550
Size: 5360
SmaI CCC'GGG
Cuts at: 1027 2189 2382 1027
Size: 1162 193 4005
Fragments arranged by size:
4005 1162 193
SmlI C'TyrA_G
Cuts at: 1063 1369 1810 2515 3430 3922 4184 1063
Size: 306 441 705 915 492 262 2239
Fragments arranged by size:
2239 915 705 492 441 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 1056 2050 2413 2913 1056
Size: 994 363 500 3503
Fragments arranged by size:
3503 994 500 363
SrfI GCCC'GGGC
Cuts at: 2189 2189
Size: 5360
StyI C'CwwG_G
Cuts at: 1562 2494 2848 1562
Size: 932 354 4074
Fragments arranged by size:
4074 932 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 823 1650 2943 3076 3535 3744 373
Size: 450 827 1293 133 459 209 1989
Fragments arranged by size:
1989 1293 827 459 450 209 133
TatI w'GTAC_w
Cuts at: 188 254 1736 4875 188
Size: 66 1482 3139 673
Fragments arranged by size:
3139 1482 673 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1427 1459 4877 4909 1427
Size: 32 3418 32 1878
Fragments arranged by size:
3418 1878 32 32
Tth111I GACn'n_nGTC
Cuts at: 3157 3157
Size: 5360
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1428 1569 2855 1428
Size: 141 1286 3933
Fragments arranged by size:
3933 1286 141
XmaI C'CCGG_G
Cuts at: 1025 2187 2380 1025
Size: 1162 193 4005
Fragments arranged by size:
4005 1162 193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BaeI BbsI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI