Restriction map for
pF1KF0007
(Circular) (Six-base) MAPSORT of: pF1KF0007.seq Check: 4685 from: 1 to: 5183
pF1KF0007 5183 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 459 2016 459
Size: 1557 3626
AccI GT'mk_AC
Cuts at: 2221 2221
Size: 5183
Acc65I G'GTAC_C
Cuts at: 2199 2199
Size: 5183
AclI AA'CG_TT
Cuts at: 5023 5023
Size: 5183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 322 952 976 1172 1348 3008 3440 3793
Size: 156 630 24 196 176 1660 432 353
Cuts at: 3793 4455 166
Size: 662 894
Fragments arranged by size:
1660 894 662 630 432 353 196 176
156 24
AfeI AGC'GCT
Cuts at: 1099 1321 1099
Size: 222 4961
AflIII A'CryG_T
Cuts at: 573 1527 2535 4335 4616 573
Size: 954 1008 1800 281 1140
Fragments arranged by size:
1800 1140 1008 954 281
AlwNI CAG_nnn'CTG
Cuts at: 285 672 717 1024 1767 1974 2488 3926 285
Size: 387 45 307 743 207 514 1438 1542
Fragments arranged by size:
1542 1438 743 514 387 307 207 45
ApaI G_GGCC'C
Cuts at: 1512 1845 2036 1512
Size: 333 191 4659
Fragments arranged by size:
4659 333 191
ApaLI G'TGCA_C
Cuts at: 4021 4021
Size: 5183
ApoI r'AATT_y
Cuts at: 1879 2187 4536 4792 1879
Size: 308 2349 256 2270
Fragments arranged by size:
2349 2270 308 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
AvaI C'yCGr_G
Cuts at: 1838 2203 1838
Size: 365 4818
BamHI G'GATC_C
Cuts at: 2151 2208 2151
Size: 57 5126
BanI G'GyrC_C
Cuts at: 314 358 2097 2199 2613 2861 2896 314
Size: 44 1739 102 414 248 35 2601
Fragments arranged by size:
2601 1739 414 248 102 44 35
BanII G_rGCy'C
Cuts at: 1472 1512 1745 1845 2036 2197 3227 1472
Size: 40 233 100 191 161 1030 3428
Fragments arranged by size:
3428 1030 233 191 161 100 40
BbeI G_GCGC'C
Cuts at: 2617 2865 2617
Size: 248 4935
BbsI GAAGACnn'nnnn_
Cuts at: 1893 1893
Size: 5183
BbvCI CC'TCA_GC
Cuts at: 512 1001 1421 512
Size: 489 420 4274
Fragments arranged by size:
4274 489 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5149
BciVI GTATCCnnnnn_n'
Cuts at: 3081 4137 5183 3081
Size: 1056 1046 3081
Fragments arranged by size:
3081 1056 1046
BclI T'GATC_A
Cuts at: 1026 2703 1026
Size: 1677 3506
BglI GCCn_nnn'nGGC
Cuts at: 388 1285 1507 388
Size: 897 222 4064
Fragments arranged by size:
4064 897 222
BglII A'GATC_T
Cuts at: 2698 4735 2698
Size: 2037 3146
BlpI GC'TnA_GC
Cuts at: 172 538 1430 1801 2295 172
Size: 366 892 371 494 3060
Fragments arranged by size:
3060 892 494 371 366
Bme1580I G_kGCm'C
Cuts at: 317 1254 1512 1821 1845 2036 2808 2901 4025
Size: 937 258 309 24 191 772 93 1124
Cuts at: 4025 317
Size: 1475
Fragments arranged by size:
1475 1124 937 772 309 258 191 93
24
BmrI ACTGGGnnnn_n'
Cuts at: 687 696 2395 2476 2811 4955 687
Size: 9 1699 81 335 2144 915
Fragments arranged by size:
2144 1699 915 335 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 262 394 481 649 919 1063 1102 1186
Size: 27 132 87 168 270 144 39 84
Cuts at: 1186 1612 1890 235
Size: 426 278 3528
Fragments arranged by size:
3528 426 278 270 168 144 132 87
84 39 27
Bpu10I CC'TnA_GC
Cuts at: 475 512 862 1001 1421 1667 475
Size: 37 350 139 420 246 3991
Fragments arranged by size:
3991 420 350 246 139 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 861 2359 3238 3952 4250 793
Size: 68 1498 879 714 298 1726
Fragments arranged by size:
1726 1498 879 714 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 4887 4887
Size: 5183
BsaAI yAC'GTr
Cuts at: 1530 2536 1530
Size: 1006 4177
BsaBI GATnn'nnATC
Cuts at: 6 2697 4734 6
Size: 2691 2037 455
Fragments arranged by size:
2691 2037 455
BsaHI Gr'CG_yC
Cuts at: 415 2614 2862 3564 5060 415
Size: 2199 248 702 1496 538
Fragments arranged by size:
2199 1496 702 538 248
BsaWI w'CCGG_w
Cuts at: 317 1780 2372 2893 3571 3982 4129 4403 317
Size: 1463 592 521 678 411 147 274 1097
Fragments arranged by size:
1463 1097 678 592 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1570 1600 1570
Size: 30 5153
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1471 1492 1618 1810 1979 1471
Size: 21 126 192 169 4675
Fragments arranged by size:
4675 192 169 126 21
BseYI C'CCAG_C
Cuts at: 391 710 941 1993 2608 3366 4031 4439 391
Size: 319 231 1052 615 758 665 408 1135
Fragments arranged by size:
1135 1052 758 665 615 408 319 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1811 1994 2024 1508
Size: 303 183 30 4667
Fragments arranged by size:
4667 303 183 30
BsiEI CG_ry'CG
Cuts at: 104 2725 2771 4001 104
Size: 2621 46 1230 1286
Fragments arranged by size:
2621 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 679 839 1472 2197 2975 3165 4025 679
Size: 160 633 725 778 190 860 1837
Fragments arranged by size:
1837 860 778 725 633 190 160
BsmI GAATG_Cn'
Cuts at: 1750 1750
Size: 5183
BsmBI CGTCTCn'nnnn_
Cuts at: 1453 1453
Size: 5183
Bsp1286I G_dGCh'C
Cuts at: 317 679 839 1254 1472 1512 1745 1821 1845
Size: 362 160 415 218 40 233 76 24
Cuts at: 1845 2036 2197 2808 2901 2975 3165 3227 4025
Size: 191 161 611 93 74 190 62 798
Cuts at: 4025 317
Size: 1475
Fragments arranged by size:
1475 798 611 415 362 233 218 191
190 161 160 93 76 74 62 40
24
BspHI T'CATG_A
Cuts at: 3615 3615
Size: 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 459 749 2016 2233 2447 2749 3130 459
Size: 290 1267 217 214 302 381 2512
Fragments arranged by size:
2512 1267 381 302 290 217 214
BsrBI CCG'CTC
Cuts at: 50 323 1194 1371 3475 3529 5013 5178 50
Size: 273 871 177 2104 54 1484 165 55
Fragments arranged by size:
2104 1484 871 273 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 738 3095 738
Size: 2357 2826
BsrFI r'CCGG_y
Cuts at: 135 2136 3181 3362 135
Size: 2001 1045 181 1956
Fragments arranged by size:
2001 1956 1045 181
BsrGI T'GTAC_A
Cuts at: 1769 1769
Size: 5183
BssSI C'ACGA_G
Cuts at: 1503 3454 4162 1503
Size: 1951 708 2524
Fragments arranged by size:
2524 1951 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2560 2560
Size: 5183
BstBI TT'CG_AA
Cuts at: 3544 3544
Size: 5183
BstXI CCAn_nnnn'nTGG
Cuts at: 1141 1141
Size: 5183
BstYI r'GATC_y
Cuts at: 1 207 226 641 1130 2151 2166 2208 2675
Size: 206 19 415 489 1021 15 42 467
Cuts at: 2675 2698 3033 3279 3585 3597 3683 4735 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1021 489 467 449 415 335 306
246 206 86 42 23 19 15 12
Bsu36I CC'TnA_GG
Cuts at: 1811 1811
Size: 5183
BtgI C'CryG_G
Cuts at: 386 636 912 1140 386
Size: 250 276 228 4429
Fragments arranged by size:
4429 276 250 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3133 3314 3477 3133
Size: 181 163 4839
Fragments arranged by size:
4839 181 163
BtsI GCAGTG_nn'
Cuts at: 2426 2426
Size: 5183
DraI TTT'AAA
Cuts at: 2182 2182
Size: 5183
DrdI GACnn_nn'nnGTC
Cuts at: 1624 2889 4233 1624
Size: 1265 1344 2574
Fragments arranged by size:
2574 1344 1265
EaeI y'GGCC_r
Cuts at: 1499 2157 2768 2942 3333 3360 4705 1499
Size: 658 611 174 391 27 1345 1977
Fragments arranged by size:
1977 1345 658 611 391 174 27
EagI C'GGCC_G
Cuts at: 2768 2768
Size: 5183
EarI CTCTTCn'nnn_
Cuts at: 298 1916 3206 3416 298
Size: 1618 1290 210 2065
Fragments arranged by size:
2065 1618 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 468 678 1234 3391 4133 4279 4993 220
Size: 248 210 556 2157 742 146 714 410
Fragments arranged by size:
2157 742 714 556 410 248 210 146
EcoICRI GAG'CTC
Cuts at: 1470 2195 1470
Size: 725 4458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 235 262 322 394 481 649 919 952
Size: 69 27 60 72 87 168 270 33
Cuts at: 952 976 1063 1102 1172 1186 1348 1612 1890
Size: 24 87 39 70 14 162 264 278
Cuts at: 1890 3008 3440 3793 4455 166
Size: 1118 432 353 662 894
Fragments arranged by size:
1118 894 662 432 353 278 270 264
168 162 87 87 72 70 69 60
39 33 27 24 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1294 1759 1294
Size: 465 4718
EcoO109I rG'GnC_Cy
Cuts at: 813 846 1404 1508 1509 1764 2033 2322 813
Size: 33 558 104 1 255 269 289 3674
Fragments arranged by size:
3674 558 289 269 255 104 33 1
EcoRI G'AATT_C
Cuts at: 2187 2187
Size: 5183
EcoRV GAT'ATC
Cuts at: 1675 1675
Size: 5183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 174 1945 3202 174
Size: 1771 1257 2155
Fragments arranged by size:
2155 1771 1257
FspI TGC'GCA
Cuts at: 2964 2964
Size: 5183
HaeII r_GCGC'y
Cuts at: 369 461 1101 1323 2617 2865 4095 4866 369
Size: 92 640 222 1294 248 1230 771 686
Fragments arranged by size:
1294 1230 771 686 640 248 222 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 531 563 1226 1258 2085 2117 3025
Size: 32 288 32 663 32 827 32 908
Cuts at: 3025 3055 3057 3087 211
Size: 30 2 30 2307
Fragments arranged by size:
2307 908 827 663 288 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2222 2222
Size: 5183
HindIII A'AGCT_T
Cuts at: 151 151
Size: 5183
KasI G'GCGC_C
Cuts at: 2613 2861 2613
Size: 248 4935
KpnI G_GTAC'C
Cuts at: 2203 2203
Size: 5183
MluI A'CGCG_T
Cuts at: 4616 4616
Size: 5183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 529 1438 2006 3942 4126 529
Size: 909 568 1936 184 1586
Fragments arranged by size:
1936 1586 909 568 184
MscI TGG'CCA
Cuts at: 1501 2159 2944 1501
Size: 658 785 3740
Fragments arranged by size:
3740 785 658
MslI CAynn'nnrTG
Cuts at: 3172 3299 3172
Size: 127 5056
MspA1I CmG'CkG
Cuts at: 171 567 717 914 1420 2294 2608 2968 3752
Size: 396 150 197 506 874 314 360 784
Cuts at: 3752 3997 171
Size: 245 1357
Fragments arranged by size:
1357 874 784 506 396 360 314 245
197 150
NaeI GCC'GGC
Cuts at: 137 2138 3364 137
Size: 2001 1226 1956
Fragments arranged by size:
2001 1956 1226
NarI GG'CG_CC
Cuts at: 2614 2862 2614
Size: 248 4935
NcoI C'CATG_G
Cuts at: 636 1140 636
Size: 504 4679
NgoMIV G'CCGG_C
Cuts at: 135 2136 3362 135
Size: 2001 1226 1956
Fragments arranged by size:
2001 1956 1226
NspI r_CATG'y
Cuts at: 907 2236 2736 4339 907
Size: 1329 500 1603 1751
Fragments arranged by size:
1751 1603 1329 500
PasI CC'CwG_GG
Cuts at: 1939 2077 1939
Size: 138 5045
PciI A'CATG_T
Cuts at: 4335 4335
Size: 5183
PflMI CCAn_nnn'nTGG
Cuts at: 285 1497 285
Size: 1212 3971
PfoI T'CCnGG_A
Cuts at: 161 530 896 1040 1685 2084 161
Size: 369 366 144 645 399 3260
Fragments arranged by size:
3260 645 399 369 366 144
PmeI GTTT'AAAC
Cuts at: 2182 2182
Size: 5183
PmlI CAC'GTG
Cuts at: 1530 1530
Size: 5183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 5151
PpuMI rG'GwC_Cy
Cuts at: 846 1404 846
Size: 558 4625
PspOMI G'GGCC_C
Cuts at: 1508 1841 2032 1508
Size: 333 191 4659
Fragments arranged by size:
4659 333 191
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1205 1237 1205
Size: 32 5151
PstI C_TGCA'G
Cuts at: 524 1298 2230 2915 524
Size: 774 932 685 2792
Fragments arranged by size:
2792 932 774 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5183
PvuII CAG'CTG
Cuts at: 171 717 2608 2968 171
Size: 546 1891 360 2386
Fragments arranged by size:
2386 1891 546 360
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 5183
SacI G_AGCT'C
Cuts at: 1472 2197 1472
Size: 725 4458
SacII CC_GC'GG
Cuts at: 915 915
Size: 5183
SalI G'TCGA_C
Cuts at: 2220 2220
Size: 5183
SanDI GG'GwC_CC
Cuts at: 846 846
Size: 5183
SapI GCTCTTCn'nnn_
Cuts at: 1916 3206 3416 1916
Size: 1290 210 3683
Fragments arranged by size:
3683 1290 210
SbfI CC_TGCA'GG
Cuts at: 2230 2230
Size: 5183
ScaI AGT'ACT
Cuts at: 706 4700 706
Size: 3994 1189
SfcI C'TryA_G
Cuts at: 33 231 520 1294 2226 2911 3879 4070 33
Size: 198 289 774 932 685 968 191 1146
Fragments arranged by size:
1146 968 932 774 685 289 198 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1507 1507
Size: 5183
SfoI GGC'GCC
Cuts at: 2615 2863 2615
Size: 248 4935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
SmaI CCC'GGG
Cuts at: 1840 2205 1840
Size: 365 4818
SmlI C'TyrA_G
Cuts at: 808 840 2338 3253 3967 4229 808
Size: 32 1498 915 714 262 1762
Fragments arranged by size:
1762 1498 915 714 262 32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5183
SphI G_CATG'C
Cuts at: 907 2236 2736 907
Size: 1329 500 3354
Fragments arranged by size:
3354 1329 500
StyI C'CwwG_G
Cuts at: 461 584 636 1140 2317 2671 461
Size: 123 52 504 1177 354 2973
Fragments arranged by size:
2973 1177 504 354 123 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 437 2177 2766 2899 3358 3567 437
Size: 1740 589 133 459 209 2053
Fragments arranged by size:
2053 1740 589 459 209 133
TatI w'GTAC_w
Cuts at: 704 1769 4698 704
Size: 1065 2929 1189
Fragments arranged by size:
2929 1189 1065
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 562 594 1570 1602 4700 4732 562
Size: 32 976 32 3098 32 1013
Fragments arranged by size:
3098 1013 976 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2980 2980
Size: 5183
XbaI T'CTAG_A
Cuts at: 2214 2214
Size: 5183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 286 1752 2678 286
Size: 1466 926 2791
Fragments arranged by size:
2791 1466 926
XmaI C'CCGG_G
Cuts at: 1838 2203 1838
Size: 365 4818
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BspEI BssHII
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI