Restriction map for
pF1KF0017
(Circular) (Six-base) MAPSORT of: pF1KF0017.seq Check: 8423 from: 1 to: 4295
pF1KF0017 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 667 667
Size: 4295
AatII G_ACGT'C
Cuts at: 386 386
Size: 4295
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 442 1311 442
Size: 869 3426
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 2120 2552 2905 3567 235
Size: 1885 432 353 662 963
Fragments arranged by size:
1885 963 662 432 353
AfeI AGC'GCT
Cuts at: 742 742
Size: 4295
AflIII A'CryG_T
Cuts at: 665 1647 3447 3728 665
Size: 982 1800 281 1232
Fragments arranged by size:
1800 1232 982 281
AlwNI CAG_nnn'CTG
Cuts at: 195 213 549 1600 3038 195
Size: 18 336 1051 1438 1452
Fragments arranged by size:
1452 1438 1051 336 18
ApaLI G'TGCA_C
Cuts at: 3133 3133
Size: 4295
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 787 1315 787
Size: 528 3767
BamHI G'GATC_C
Cuts at: 462 1320 462
Size: 858 3437
BanI G'GyrC_C
Cuts at: 179 442 1311 1725 1973 2008 179
Size: 263 869 414 248 35 2466
Fragments arranged by size:
2466 869 414 263 248 35
BanII G_rGCy'C
Cuts at: 131 1079 1309 2339 131
Size: 948 230 1030 2087
Fragments arranged by size:
2087 1030 948 230
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 633 934 633
Size: 301 3994
BciVI GTATCCnnnnn_n'
Cuts at: 355 2193 3249 4295 355
Size: 1838 1056 1046 355
Fragments arranged by size:
1838 1056 1046 355
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 139 1407 139
Size: 1268 3027
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3137 1920
Size: 93 1124 3078
Fragments arranged by size:
3078 1124 93
BmgBI CAC'GTC
Cuts at: 932 932
Size: 4295
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1588 1923 4067 1507
Size: 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 852 1047 2662 852
Size: 195 1615 2485
Fragments arranged by size:
2485 1615 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 469 1093 217
Size: 252 624 3419
Fragments arranged by size:
3419 624 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1042 1471 2350 3064 3362 892
Size: 150 429 879 714 298 1825
Fragments arranged by size:
1825 879 714 429 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 1260 3999 1260
Size: 2739 1556
BsaAI yAC'GTr
Cuts at: 666 1648 666
Size: 982 3313
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 383 638 1726 1974 2676 4172 383
Size: 255 1088 248 702 1496 506
Fragments arranged by size:
1496 1088 702 506 255 248
BsaWI w'CCGG_w
Cuts at: 590 1484 2005 2683 3094 3241 3515 590
Size: 894 521 678 411 147 274 1370
Fragments arranged by size:
1370 894 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 283
Size: 4295
BseYI C'CCAG_C
Cuts at: 1720 2478 3143 3551 1720
Size: 758 665 408 2464
Fragments arranged by size:
2464 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1057
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3113 104
Size: 1733 46 1230 1286
Fragments arranged by size:
1733 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 365 1079 1309 2087 2277 3137 365
Size: 714 230 778 190 860 1523
Fragments arranged by size:
1523 860 778 714 230 190
BsmBI CGTCTCn'nnnn_
Cuts at: 145 145
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 131 365 1079 1309 1920 2013 2087 2277 2339
Size: 234 714 230 611 93 74 190 62
Cuts at: 2339 3137 131
Size: 798 1289
Fragments arranged by size:
1289 798 714 611 234 230 190 93
74 62
BspHI T'CATG_A
Cuts at: 2727 2727
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 482 667 791 1278 1345 1559 1861 2242
Size: 14 185 124 487 67 214 302 381
Cuts at: 2242 468
Size: 2521
Fragments arranged by size:
2521 487 381 302 214 185 124 67
14
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 864 2207 392
Size: 472 1343 2480
Fragments arranged by size:
2480 1343 472
BsrFI r'CCGG_y
Cuts at: 2293 2474 2293
Size: 181 4114
BssSI C'ACGA_G
Cuts at: 505 2566 3274 505
Size: 2061 708 1526
Fragments arranged by size:
2061 1526 708
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1672 369
Size: 1303 2992
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstYI r'GATC_y
Cuts at: 1 462 1320 1787 1810 2145 2391 2697 2709
Size: 461 858 467 23 335 246 306 12
Cuts at: 2709 2795 3847 1
Size: 86 1052 449
Fragments arranged by size:
1052 858 467 461 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 538 845 538
Size: 307 3988
BtgI C'CryG_G
Cuts at: 107 131 759 1003 107
Size: 24 628 244 3399
Fragments arranged by size:
3399 628 244 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1538
Size: 4295
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 3345 2001
Size: 1344 2951
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 152 814 2318 2528 152
Size: 662 1504 210 1919
Fragments arranged by size:
1919 1504 662 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 3245 3391 4105 2503
Size: 742 146 714 2693
Fragments arranged by size:
2693 742 714 146
EcoICRI GAG'CTC
Cuts at: 1077 1307 1077
Size: 230 4065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 235 469 1093 2120 2552 2905 3567 217
Size: 18 234 624 1027 432 353 662 945
Fragments arranged by size:
1027 945 662 624 432 353 234 18
EcoNI CCTnn'n_nnAGG
Cuts at: 529 787 529
Size: 258 4037
EcoO109I rG'GnC_Cy
Cuts at: 420 480 534 1434 420
Size: 60 54 900 3281
Fragments arranged by size:
3281 900 60 54
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 744 945 1729 1977 3207 3978 744
Size: 201 784 248 1230 771 1061
Fragments arranged by size:
1230 1061 784 771 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 608 640 1211 1240 1243 1272 2137 2167 2169
Size: 32 571 29 3 29 865 30 2
Cuts at: 2169 2199 608
Size: 30 2704
Fragments arranged by size:
2704 865 571 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 446 1315 446
Size: 869 3426
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3054 3238 3054
Size: 184 4111
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 685 2284 2411 685
Size: 1599 127 2569
Fragments arranged by size:
2569 1599 127
MspA1I CmG'CkG
Cuts at: 133 146 761 798 1406 1720 2080 2864 3109
Size: 13 615 37 608 314 360 784 245
Cuts at: 3109 133
Size: 1319
Fragments arranged by size:
1319 784 615 608 360 314 245 37
13
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 1348 1848 3451 1348
Size: 500 1603 2192
Fragments arranged by size:
2192 1603 500
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4295
PfoI T'CCnGG_A
Cuts at: 194 386 719 194
Size: 192 333 3770
Fragments arranged by size:
3770 333 192
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 480 480
Size: 4295
PstI C_TGCA'G
Cuts at: 479 1342 2027 479
Size: 863 685 2747
Fragments arranged by size:
2747 863 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 798 1720 2080 798
Size: 922 360 3013
Fragments arranged by size:
3013 922 360
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1079 1309 1079
Size: 230 4065
SacII CC_GC'GG
Cuts at: 134 762 134
Size: 628 3667
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 479 1342 479
Size: 863 3432
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SexAI A'CCwGG_T
Cuts at: 1250 1250
Size: 4295
SfcI C'TryA_G
Cuts at: 33 475 1338 2023 2991 3182 33
Size: 442 863 685 968 191 1146
Fragments arranged by size:
1146 968 863 685 442 191
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 907 1021 1450 2365 3079 3341 907
Size: 114 429 915 714 262 1861
Fragments arranged by size:
1861 915 714 429 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 490 604 1114 490
Size: 114 510 3671
Fragments arranged by size:
3671 510 114
StyI C'CwwG_G
Cuts at: 107 338 1429 1783 107
Size: 231 1091 354 2619
Fragments arranged by size:
2619 1091 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 137 973 1138 1878 2011 2470 2679 137
Size: 836 165 740 133 459 209 1753
Fragments arranged by size:
1753 836 740 459 209 165 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 1252 2092 1252
Size: 840 3455
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1790 1790
Size: 4295
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 808 1063 808
Size: 255 4040
ZraI GAC'GTC
Cuts at: 384 384
Size: 4295
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI