Restriction map for
pF1KF0021
(Circular) (Six-base) MAPSORT of: pF1KF0021.seq Check: 2257 from: 1 to: 4601
pF1KF0021 4601 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 615 615
Size: 4601
AatII G_ACGT'C
Cuts at: 788 1148 788
Size: 360 4241
AccI GT'mk_AC
Cuts at: 950 1639 950
Size: 689 3912
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2858 3211 3873 2426
Size: 432 353 662 3154
Fragments arranged by size:
3154 662 432 353
AflIII A'CryG_T
Cuts at: 167 1115 1953 3753 4034 167
Size: 948 838 1800 281 734
Fragments arranged by size:
1800 948 838 734 281
AgeI A'CCGG_T
Cuts at: 695 695
Size: 4601
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 4601
AleI CACnn'nnGTG
Cuts at: 292 1321 292
Size: 1029 3572
AlwNI CAG_nnn'CTG
Cuts at: 813 1906 3344 813
Size: 1093 1438 2070
Fragments arranged by size:
2070 1438 1093
ApaI G_GGCC'C
Cuts at: 710 710
Size: 4601
ApaLI G'TGCA_C
Cuts at: 3439 3439
Size: 4601
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 918 1621 918
Size: 703 3898
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 896 1323 1396 1617 2031 2279 2314 896
Size: 427 73 221 414 248 35 3183
Fragments arranged by size:
3183 427 414 248 221 73 35
BanII G_rGCy'C
Cuts at: 710 1615 2645 710
Size: 905 1030 2666
Fragments arranged by size:
2666 1030 905
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 799 1185 1255 799
Size: 386 70 4145
Fragments arranged by size:
4145 386 70
BbvCI CC'TCA_GC
Cuts at: 1579 1579
Size: 4601
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3555 4601 2499
Size: 1056 1046 2499
Fragments arranged by size:
2499 1056 1046
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglII A'GATC_T
Cuts at: 2116 4153 2116
Size: 2037 2564
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 190 611 710 881 899 1399 2226 2319 3443
Size: 421 99 171 18 500 827 93 1124
Cuts at: 3443 190
Size: 1348
Fragments arranged by size:
1348 1124 827 500 421 171 99 93
18
BmgBI CAC'GTC
Cuts at: 460 460
Size: 4601
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 1381
Size: 4601
Bpu10I CC'TnA_GC
Cuts at: 1579 1579
Size: 4601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1777 2656 3370 3668 337
Size: 1440 879 714 298 1270
Fragments arranged by size:
1440 1270 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 575 1579 4305 121
Size: 454 1004 2726 417
Fragments arranged by size:
2726 1004 454 417
BsaAI yAC'GTr
Cuts at: 1041 1277 1954 1041
Size: 236 677 3688
Fragments arranged by size:
3688 677 236
BsaBI GATnn'nnATC
Cuts at: 6 871 2115 4152 6
Size: 865 1244 2037 455
Fragments arranged by size:
2037 1244 865 455
BsaHI Gr'CG_yC
Cuts at: 725 785 1145 2032 2280 2982 4478 725
Size: 60 360 887 248 702 1496 848
Fragments arranged by size:
1496 887 848 702 360 248 60
BsaWI w'CCGG_w
Cuts at: 695 926 1790 2311 2989 3400 3547 3821 695
Size: 231 864 521 678 411 147 274 1475
Fragments arranged by size:
1475 864 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 361
Size: 30 4571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 366 373 748 751 990 1057 366
Size: 7 375 3 239 67 3910
Fragments arranged by size:
3910 375 239 67 7 3
BseYI C'CCAG_C
Cuts at: 701 1454 2026 2784 3449 3857 701
Size: 753 572 758 665 408 1445
Fragments arranged by size:
1445 758 753 665 572 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1332 472
Size: 860 3741
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3419 104
Size: 2039 46 1230 1286
Fragments arranged by size:
2039 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1615 2393 2583 3443 1615
Size: 778 190 860 2773
Fragments arranged by size:
2773 860 778 190
BsmI GAATG_Cn'
Cuts at: 214 214
Size: 4601
BsmBI CGTCTCn'nnnn_
Cuts at: 450 1002 450
Size: 552 4049
Bsp1286I G_dGCh'C
Cuts at: 190 611 710 881 899 1399 1615 2226 2319
Size: 421 99 171 18 500 216 611 93
Cuts at: 2319 2393 2583 2645 3443 190
Size: 74 190 62 798 1348
Fragments arranged by size:
1348 798 611 500 421 216 190 171
99 93 74 62 18
BspEI T'CCGG_A
Cuts at: 926 926
Size: 4601
BspHI T'CATG_A
Cuts at: 269 848 3033 269
Size: 579 2185 1837
Fragments arranged by size:
2185 1837 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 615 640 812 1651 1865 2167 2548 615
Size: 25 172 839 214 302 381 2668
Fragments arranged by size:
2668 839 381 302 214 172 25
BsrBI CCG'CTC
Cuts at: 50 423 2893 2947 4431 4596 50
Size: 373 2470 54 1484 165 55
Fragments arranged by size:
2470 1484 373 165 55 54
BsrDI GCAATG_nn'
Cuts at: 654 1220 2513 654
Size: 566 1293 2742
Fragments arranged by size:
2742 1293 566
BsrFI r'CCGG_y
Cuts at: 695 1399 2599 2780 695
Size: 704 1200 181 2516
Fragments arranged by size:
2516 1200 704 181
BsrGI T'GTAC_A
Cuts at: 164 1118 164
Size: 954 3647
BssHII G'CGCG_C
Cuts at: 1135 1135
Size: 4601
BssSI C'ACGA_G
Cuts at: 2872 3580 2872
Size: 708 3893
BstAPI GCAn_nnn'nTGC
Cuts at: 975 1978 975
Size: 1003 3598
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstEII G'GTnAC_C
Cuts at: 627 627
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 1320 1320
Size: 4601
BstYI r'GATC_y
Cuts at: 1 463 809 1626 2093 2116 2451 2697 3003
Size: 462 346 817 467 23 335 246 306
Cuts at: 3003 3015 3101 4153 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 817 467 462 449 346 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 305 1095 1282 107
Size: 198 790 187 3426
Fragments arranged by size:
3426 790 198 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 410 2551 2732 2895 410
Size: 2141 181 163 2116
Fragments arranged by size:
2141 2116 181 163
BtsI GCAGTG_nn'
Cuts at: 221 1437 1844 221
Size: 1216 407 2978
Fragments arranged by size:
2978 1216 407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 273 308 273
Size: 35 4566
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3651 2307
Size: 1344 3257
EaeI y'GGCC_r
Cuts at: 1157 1565 2186 2360 2751 2778 4123 1157
Size: 408 621 174 391 27 1345 1635
Fragments arranged by size:
1635 1345 621 408 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 150 331 1189 1261 2624 2834 150
Size: 181 858 72 1363 210 1917
Fragments arranged by size:
1917 1363 858 210 181 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1492 2809 3551 3697 4411 1492
Size: 1317 742 146 714 1682
Fragments arranged by size:
1682 1317 742 714 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2426 2858 3211 3873 1381
Size: 1045 432 353 662 2109
Fragments arranged by size:
2109 1045 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 355 622 355
Size: 267 4334
EcoO109I rG'GnC_Cy
Cuts at: 706 707 1385 1493 1740 706
Size: 1 678 108 247 3567
Fragments arranged by size:
3567 678 247 108 1
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1272 2620 1272
Size: 1348 3253
FspI TGC'GCA
Cuts at: 1237 2382 1237
Size: 1145 3456
HaeII r_GCGC'y
Cuts at: 641 1011 2035 2283 3513 4284 641
Size: 370 1024 248 1230 771 958
Fragments arranged by size:
1230 1024 958 771 370 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 361 390 393 738 770 1348 1380 2443
Size: 3 29 3 345 32 578 32 1063
Cuts at: 2443 2473 2475 2505 358
Size: 30 2 30 2454
Fragments arranged by size:
2454 1063 578 345 32 32 30 30
29 3 3 2
HincII GTy'rAC
Cuts at: 543 1640 543
Size: 1097 3504
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3360 3544 3360
Size: 184 4417
MscI TGG'CCA
Cuts at: 2362 2362
Size: 4601
MslI CAynn'nnrTG
Cuts at: 292 625 853 1114 1225 1321 2590 2717 292
Size: 333 228 261 111 96 1269 127 2176
Fragments arranged by size:
2176 1269 333 261 228 127 111 96
MspA1I CmG'CkG
Cuts at: 1097 1162 1458 1712 2026 2386 3170 3415 1097
Size: 65 296 254 314 360 784 245 2283
Fragments arranged by size:
2283 784 360 314 296 254 245 65
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 305 107
Size: 198 4403
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NspI r_CATG'y
Cuts at: 171 1119 1654 2154 3757 171
Size: 948 535 500 1603 1015
Fragments arranged by size:
1603 1015 948 535 500
PciI A'CATG_T
Cuts at: 167 1115 3753 167
Size: 948 2638 1015
Fragments arranged by size:
2638 1015 948
PflMI CCAn_nnn'nTGG
Cuts at: 1359 1359
Size: 4601
PfoI T'CCnGG_A
Cuts at: 407 1031 407
Size: 624 3977
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 1493 1493
Size: 4601
PspOMI G'GGCC_C
Cuts at: 706 706
Size: 4601
PstI C_TGCA'G
Cuts at: 315 637 809 1648 2333 315
Size: 322 172 839 685 2583
Fragments arranged by size:
2583 839 685 322 172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 1458 2026 2386 1458
Size: 568 360 3673
Fragments arranged by size:
3673 568 360
RsrII CG'GwC_CG
Cuts at: 766 2796 766
Size: 2030 2571
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SacII CC_GC'GG
Cuts at: 1098 1098
Size: 4601
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 311 633 805 1644 2329 3297 3488 33
Size: 278 322 172 839 685 968 191 1146
Fragments arranged by size:
1146 968 839 685 322 278 191 172
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 352 1756 2671 3385 3647 352
Size: 1404 915 714 262 1306
Fragments arranged by size:
1404 1306 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 107 209 305 1735 2089 107
Size: 102 96 1430 354 2619
Fragments arranged by size:
2619 1430 354 102 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1366 1468 1506 2184 2317 2776 2985 1275
Size: 91 102 38 678 133 459 209 2891
Fragments arranged by size:
2891 678 459 209 133 102 91 38
TatI w'GTAC_w
Cuts at: 164 1118 4116 164
Size: 954 2998 649
Fragments arranged by size:
2998 954 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 1297 2398 1297
Size: 1101 3500
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1317 1462 2096 1317
Size: 145 634 3822
Fragments arranged by size:
3822 634 145
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
ZraI GAC'GTC
Cuts at: 786 1146 786
Size: 360 4241
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BcgI
BfrBI BglI BmtI BsiWI BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI