Restriction map for
pF1KF0034
(Circular) (Six-base) MAPSORT of: pF1KF0034.seq Check: 1157 from: 1 to: 7049
pF1KF0034 7049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 777 1476 777
Size: 699 6350
AccI GT'mk_AC
Cuts at: 4087 4087
Size: 7049
Acc65I G'GTAC_C
Cuts at: 4065 4065
Size: 7049
AclI AA'CG_TT
Cuts at: 6889 6889
Size: 7049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2953 3751 4874 5306 5659 6321 2953
Size: 798 1123 432 353 662 3681
Fragments arranged by size:
3681 1123 798 662 432 353
AfeI AGC'GCT
Cuts at: 1585 1597 3925 1585
Size: 12 2328 4709
Fragments arranged by size:
4709 2328 12
AflIII A'CryG_T
Cuts at: 4401 6201 6482 4401
Size: 1800 281 4968
Fragments arranged by size:
4968 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1446 1446
Size: 7049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3248 3922 3248
Size: 674 6375
AlwNI CAG_nnn'CTG
Cuts at: 566 3101 3486 3556 4354 5792 566
Size: 2535 385 70 798 1438 1823
Fragments arranged by size:
2535 1823 1438 798 385 70
ApaI G_GGCC'C
Cuts at: 658 1428 2528 2757 3977 4004 658
Size: 770 1100 229 1220 27 3703
Fragments arranged by size:
3703 1220 1100 770 229 27
ApaLI G'TGCA_C
Cuts at: 5887 5887
Size: 7049
ApoI r'AATT_y
Cuts at: 2701 4053 6402 6658 2701
Size: 1352 2349 256 3092
Fragments arranged by size:
3092 2349 1352 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
AvaI C'yCGr_G
Cuts at: 128 1133 1429 1913 1980 2054 2252 2367 2396
Size: 1005 296 484 67 74 198 115 29
Cuts at: 2396 3130 3309 4020 4069 128
Size: 734 179 711 49 3108
Fragments arranged by size:
3108 1005 734 711 484 296 198 179
115 74 67 49 29
AvrII C'CTAG_G
Cuts at: 1494 1494
Size: 7049
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 422 455 422
Size: 33 7016
BamHI G'GATC_C
Cuts at: 420 4074 420
Size: 3654 3395
BanI G'GyrC_C
Cuts at: 1477 1611 1720 1994 2097 2360 2511 2971 4065
Size: 134 109 274 103 263 151 460 1094
Cuts at: 4065 4479 4727 4762 1477
Size: 414 248 35 3764
Fragments arranged by size:
3764 1094 460 414 274 263 248 151
134 109 103 35
BanII G_rGCy'C
Cuts at: 332 658 866 1066 1316 1428 2528 2689 2757
Size: 326 208 200 250 112 1100 161 68
Cuts at: 2757 3977 4004 4063 5093 332
Size: 1220 27 59 1030 2288
Fragments arranged by size:
2288 1220 1100 1030 326 250 208 200
161 112 68 59 27
BbeI G_GCGC'C
Cuts at: 1481 1615 2101 2364 2515 4483 4731 1481
Size: 134 486 263 151 1968 248 3799
Fragments arranged by size:
3799 1968 486 263 248 151 134
BbsI GAAGACnn'nnnn_
Cuts at: 142 959 1155 142
Size: 817 196 6036
Fragments arranged by size:
6036 817 196
BbvCI CC'TCA_GC
Cuts at: 497 1406 3511 497
Size: 909 2105 4035
Fragments arranged by size:
4035 2105 909
BciVI GTATCCnnnnn_n'
Cuts at: 423 4947 6003 7049 423
Size: 4524 1056 1046 423
Fragments arranged by size:
4524 1056 1046 423
BclI T'GATC_A
Cuts at: 4569 4569
Size: 7049
BglI GCCn_nnn'nGGC
Cuts at: 1346 1378 1699 1892 3460 3981 1346
Size: 32 321 193 1568 521 4414
Fragments arranged by size:
4414 1568 521 321 193 32
BglII A'GATC_T
Cuts at: 3854 4564 6601 3854
Size: 710 2037 4302
Fragments arranged by size:
4302 2037 710
BlpI GC'TnA_GC
Cuts at: 4161 4161
Size: 7049
Bme1580I G_kGCm'C
Cuts at: 355 542 624 643 658 1428 1723 2528 2757
Size: 187 82 19 15 770 295 805 229
Cuts at: 2757 3563 3977 4004 4674 4767 5891 355
Size: 806 414 27 670 93 1124 1513
Fragments arranged by size:
1513 1124 806 805 770 670 414 295
229 187 93 82 27 19 15
BmgBI CAC'GTC
Cuts at: 3671 3671
Size: 7049
BmrI ACTGGGnnnn_n'
Cuts at: 3880 4261 4342 4677 6821 3880
Size: 381 81 335 2144 4108
Fragments arranged by size:
4108 2144 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 1258 1722 2742 5416 837
Size: 421 464 1020 2674 2470
Fragments arranged by size:
2674 2470 1020 464 421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 588 3576 505
Size: 83 2988 3978
Fragments arranged by size:
3978 2988 83
Bpu10I CC'TnA_GC
Cuts at: 497 1036 1406 2285 2556 3511 497
Size: 539 370 879 271 955 4035
Fragments arranged by size:
4035 955 879 539 370 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3516 4225 5104 5818 6116 3516
Size: 709 879 714 298 4449
Fragments arranged by size:
4449 879 714 709 298
BsaI GGTCTCn'nnnn_
Cuts at: 176 997 1212 2414 3123 6753 176
Size: 821 215 1202 709 3630 472
Fragments arranged by size:
3630 1202 821 709 472 215
BsaAI yAC'GTr
Cuts at: 4402 4402
Size: 7049
BsaBI GATnn'nnATC
Cuts at: 6 3725 4563 6600 6
Size: 3719 838 2037 455
Fragments arranged by size:
3719 2037 838 455
BsaHI Gr'CG_yC
Cuts at: 1478 1612 2098 2361 2512 2696 3413 4480 4728
Size: 134 486 263 151 184 717 1067 248
Cuts at: 4728 5430 6926 1478
Size: 702 1496 1601
Fragments arranged by size:
1601 1496 1067 717 702 486 263 248
184 151 134
BsaWI w'CCGG_w
Cuts at: 837 3075 4238 4759 5437 5848 5995 6269 837
Size: 2238 1163 521 678 411 147 274 1617
Fragments arranged by size:
2238 1617 1163 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 180 210 573 603 832 862 2284 2314 2392
Size: 30 363 30 229 30 1422 30 78
Cuts at: 2392 2422 3537 3567 180
Size: 30 1115 30 3662
Fragments arranged by size:
3662 1422 1115 363 229 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 594 669 681 925 934 937 973 976
Size: 437 75 12 244 9 3 36 3
Cuts at: 976 979 1321 1324 2268 2341 2370 2373 2376
Size: 3 342 3 944 73 29 3 3
Cuts at: 2376 2379 3001 3022 3148 3160 3187 3190 3193
Size: 3 622 21 126 12 27 3 3
Cuts at: 3193 3196 3211 3214 3229 3232 3235 3778 3832
Size: 3 15 3 15 3 3 543 54
Cuts at: 3832 157
Size: 3374
Fragments arranged by size:
3374 944 622 543 437 342 244 126
75 73 54 36 29 27 21 15
15 12 12 9 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 442 541 759 880 1394 1535 1724 3151 3283
Size: 99 218 121 514 141 189 1427 132
Cuts at: 3283 3344 3549 4474 5232 5897 6305 442
Size: 61 205 925 758 665 408 1186
Fragments arranged by size:
1427 1186 925 758 665 514 408 218
205 189 141 132 121 99 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 3384 3413 3724 737
Size: 2647 29 311 4062
Fragments arranged by size:
4062 2647 311 29
BsiEI CG_ry'CG
Cuts at: 104 1350 1373 1631 1881 2047 3951 3985 4591
Size: 1246 23 258 250 166 1904 34 606
Cuts at: 4591 4637 5867 104
Size: 46 1230 1286
Fragments arranged by size:
1904 1286 1246 1230 606 258 250 166
46 34 23
BsiHKAI G_wGCw'C
Cuts at: 4063 4841 5031 5891 4063
Size: 778 190 860 5221
Fragments arranged by size:
5221 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 562 641 562
Size: 79 6970
Bsp1286I G_dGCh'C
Cuts at: 332 355 542 624 643 658 866 1066 1316
Size: 23 187 82 19 15 208 200 250
Cuts at: 1316 1428 1723 2528 2689 2757 3563 3977 4004
Size: 112 295 805 161 68 806 414 27
Cuts at: 4004 4063 4674 4767 4841 5031 5093 5891 332
Size: 59 611 93 74 190 62 798 1490
Fragments arranged by size:
1490 806 805 798 611 414 295 250
208 200 190 187 161 112 93 82
74 68 62 59 27 23 19 15
BspEI T'CCGG_A
Cuts at: 837 3075 837
Size: 2238 4811
BspHI T'CATG_A
Cuts at: 5481 5481
Size: 7049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 464 777 1476 1559 1892 3608 4099 4313
Size: 198 313 699 83 333 1716 491 214
Cuts at: 4313 4615 4996 266
Size: 302 381 2319
Fragments arranged by size:
2319 1716 699 491 381 333 313 302
214 198 83
BsrBI CCG'CTC
Cuts at: 50 1417 1775 1826 1832 1838 1844 1850 1865
Size: 1367 358 51 6 6 6 6 15
Cuts at: 1865 1992 2321 2331 2366 2751 3012 3753 5341
Size: 127 329 10 35 385 261 741 1588
Cuts at: 5341 5395 6879 7044 50
Size: 54 1484 165 55
Fragments arranged by size:
1588 1484 1367 741 385 358 329 261
165 127 55 54 51 35 15 10
6 6 6 6
BsrDI GCAATG_nn'
Cuts at: 4961 4961
Size: 7049
BsrFI r'CCGG_y
Cuts at: 868 1154 1368 1503 2231 2677 3301 5047 5228
Size: 286 214 135 728 446 624 1746 181
Cuts at: 5228 868
Size: 2689
Fragments arranged by size:
2689 1746 728 624 446 286 214 181
135
BssSI C'ACGA_G
Cuts at: 2127 5320 6028 2127
Size: 3193 708 3148
Fragments arranged by size:
3193 3148 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4426 4426
Size: 7049
BstBI TT'CG_AA
Cuts at: 5410 5410
Size: 7049
BstEII G'GTnAC_C
Cuts at: 1221 2134 2434 1221
Size: 913 300 5836
Fragments arranged by size:
5836 913 300
BstXI CCAn_nnnn'nTGG
Cuts at: 450 2226 2766 3570 3660 450
Size: 1776 540 804 90 3839
Fragments arranged by size:
3839 1776 804 540 90
BstYI r'GATC_y
Cuts at: 1 420 519 1562 3506 3854 4074 4541 4564
Size: 419 99 1043 1944 348 220 467 23
Cuts at: 4564 4899 5145 5451 5463 5549 6601 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1944 1052 1043 467 449 419 348 335
306 246 220 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 908 2520 3113 3835 908
Size: 1612 593 722 4122
Fragments arranged by size:
4122 1612 722 593
BtgI C'CryG_G
Cuts at: 107 319 383 1388 1420 1887 1998 3089 3242
Size: 212 64 1005 32 467 111 1091 153
Cuts at: 3242 3708 3979 107
Size: 466 271 3177
Fragments arranged by size:
3177 1091 1005 467 466 271 212 153
111 64 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 179 4999 5180 5343 179
Size: 4820 181 163 1885
Fragments arranged by size:
4820 1885 181 163
BtsI GCAGTG_nn'
Cuts at: 2898 2919 3254 3275 4292 2898
Size: 21 335 21 1017 5655
Fragments arranged by size:
5655 1017 335 21 21
DraI TTT'AAA
Cuts at: 4048 4048
Size: 7049
DraIII CAC_nnn'GTG
Cuts at: 2129 2129
Size: 7049
DrdI GACnn_nn'nnGTC
Cuts at: 144 2997 4755 6099 144
Size: 2853 1758 1344 1094
Fragments arranged by size:
2853 1758 1344 1094
EaeI y'GGCC_r
Cuts at: 380 1347 1370 1379 1628 1958 2657 2768 2919
Size: 967 23 9 249 330 699 111 151
Cuts at: 2919 3245 3776 3982 4634 4808 5199 5226 6571
Size: 326 531 206 652 174 391 27 1345
Cuts at: 6571 380
Size: 858
Fragments arranged by size:
1345 967 858 699 652 531 391 330
326 249 206 174 151 111 27 23
9
EagI C'GGCC_G
Cuts at: 1347 1370 1628 3982 4634 1347
Size: 23 258 2354 652 3762
Fragments arranged by size:
3762 2354 652 258 23
EarI CTCTTCn'nnn_
Cuts at: 911 923 935 941 1339 2206 2397 2568 3188
Size: 12 12 6 398 867 191 171 620
Cuts at: 3188 3200 3206 3442 5072 5282 911
Size: 12 6 236 1630 210 2678
Fragments arranged by size:
2678 1630 867 620 398 236 210 191
171 12 12 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 1327 1634 1803 1932 2962 2998 3184 3331
Size: 1166 307 169 129 1030 36 186 147
Cuts at: 3331 5257 5999 6145 6859 161
Size: 1926 742 146 714 351
Fragments arranged by size:
1926 1166 1030 742 714 351 307 186
169 147 146 129 36
EcoICRI GAG'CTC
Cuts at: 4061 4061
Size: 7049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 588 2953 3576 3751 4874 5306 5659 6321
Size: 83 2365 623 175 1123 432 353 662
Cuts at: 6321 505
Size: 1233
Fragments arranged by size:
2365 1233 1123 662 623 432 353 175
83
EcoNI CCTnn'n_nnAGG
Cuts at: 1039 2519 3130 3838 1039
Size: 1480 611 708 4250
Fragments arranged by size:
4250 1480 708 611
EcoO109I rG'GnC_Cy
Cuts at: 255 563 654 655 896 1425 2091 2181 2257
Size: 308 91 1 241 529 666 90 76
Cuts at: 2257 2295 2524 2525 2754 3963 3973 3974 4000
Size: 38 229 1 229 1209 10 1 26
Cuts at: 4000 4001 4188 255
Size: 1 187 3116
Fragments arranged by size:
3116 1209 666 529 308 241 229 229
187 91 90 76 38 26 10 1
1 1 1
EcoRI G'AATT_C
Cuts at: 4053 4053
Size: 7049
EcoRV GAT'ATC
Cuts at: 786 786
Size: 7049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5068 5068
Size: 7049
FspI TGC'GCA
Cuts at: 4830 4830
Size: 7049
HaeII r_GCGC'y
Cuts at: 309 1327 1481 1587 1599 1615 1735 1941 2101
Size: 1018 154 106 12 16 120 206 160
Cuts at: 2101 2364 2425 2515 3927 4483 4731 5961 6732
Size: 263 61 90 1412 556 248 1230 771
Cuts at: 6732 309
Size: 626
Fragments arranged by size:
1412 1230 1018 771 626 556 263 248
206 160 154 120 106 90 61 16
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 509 541 770 802 1432 1464 2053 2085 2392
Size: 32 229 32 630 32 589 32 307
Cuts at: 2392 2424 2464 2496 2656 2688 3537 3569 4891
Size: 32 40 32 160 32 849 32 1322
Cuts at: 4891 4921 4923 4953 509
Size: 30 2 30 2605
Fragments arranged by size:
2605 1322 849 630 589 307 229 160
40 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4088 4088
Size: 7049
KasI G'GCGC_C
Cuts at: 1477 1611 2097 2360 2511 4479 4727 1477
Size: 134 486 263 151 1968 248 3799
Fragments arranged by size:
3799 1968 486 263 248 151 134
KpnI G_GTAC'C
Cuts at: 4069 4069
Size: 7049
MluI A'CGCG_T
Cuts at: 6482 6482
Size: 7049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 899 1445 2270 2395 5808 5992 353
Size: 546 546 825 125 3413 184 1410
Fragments arranged by size:
3413 1410 825 546 546 184 125
MscI TGG'CCA
Cuts at: 382 2770 2921 3778 4810 382
Size: 2388 151 857 1032 2621
Fragments arranged by size:
2621 2388 1032 857 151
MslI CAynn'nnrTG
Cuts at: 2224 2764 5038 5165 2224
Size: 540 2274 127 4108
Fragments arranged by size:
4108 2274 540 127
MspA1I CmG'CkG
Cuts at: 892 1228 1422 1636 1685 1889 2000 2552 2906
Size: 336 194 214 49 204 111 552 354
Cuts at: 2906 3101 3348 3388 3410 4160 4474 4834 5618
Size: 195 247 40 22 750 314 360 784
Cuts at: 5618 5863 892
Size: 245 2078
Fragments arranged by size:
2078 784 750 552 360 354 336 314
247 245 214 204 195 194 111 49
40 22
NaeI GCC'GGC
Cuts at: 1156 1505 2233 2679 5230 1156
Size: 349 728 446 2551 2975
Fragments arranged by size:
2975 2551 728 446 349
NarI GG'CG_CC
Cuts at: 1478 1612 2098 2361 2512 4480 4728 1478
Size: 134 486 263 151 1968 248 3799
Fragments arranged by size:
3799 1968 486 263 248 151 134
NcoI C'CATG_G
Cuts at: 107 107
Size: 7049
NgoMIV G'CCGG_C
Cuts at: 1154 1503 2231 2677 5228 1154
Size: 349 728 446 2551 2975
Fragments arranged by size:
2975 2551 728 446 349
NspI r_CATG'y
Cuts at: 483 1459 4102 4602 6205 483
Size: 976 2643 500 1603 1327
Fragments arranged by size:
2643 1603 1327 976 500
PasI CC'CwG_GG
Cuts at: 1754 3970 1754
Size: 2216 4833
PciI A'CATG_T
Cuts at: 6201 6201
Size: 7049
PflMI CCAn_nnn'nTGG
Cuts at: 3374 3374
Size: 7049
PfoI T'CCnGG_A
Cuts at: 416 2177 2590 416
Size: 1761 413 4875
Fragments arranged by size:
4875 1761 413
PmeI GTTT'AAAC
Cuts at: 4048 4048
Size: 7049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5495 5527 5495
Size: 32 7017
PpuMI rG'GwC_Cy
Cuts at: 255 896 2181 2257 2295 3963 255
Size: 641 1285 76 38 1668 3341
Fragments arranged by size:
3341 1668 1285 641 76 38
PshAI GACnn'nnGTC
Cuts at: 2809 2809
Size: 7049
PspOMI G'GGCC_C
Cuts at: 654 1424 2524 2753 3973 4000 654
Size: 770 1100 229 1220 27 3703
Fragments arranged by size:
3703 1220 1100 770 229 27
PspXI vC'TCGA_Gb
Cuts at: 1980 1980
Size: 7049
PstI C_TGCA'G
Cuts at: 251 1487 2609 2959 3264 4096 4781 251
Size: 1236 1122 350 305 832 685 2519
Fragments arranged by size:
2519 1236 1122 832 685 350 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7049
PvuII CAG'CTG
Cuts at: 3101 3348 3388 4474 4834 3101
Size: 247 40 1086 360 5316
Fragments arranged by size:
5316 1086 360 247 40
RsrII CG'GwC_CG
Cuts at: 5244 5244
Size: 7049
SacI G_AGCT'C
Cuts at: 4063 4063
Size: 7049
SacII CC_GC'GG
Cuts at: 1423 1890 2001 1423
Size: 467 111 6471
Fragments arranged by size:
6471 467 111
SalI G'TCGA_C
Cuts at: 4086 4086
Size: 7049
SanDI GG'GwC_CC
Cuts at: 896 3963 896
Size: 3067 3982
SapI GCTCTTCn'nnn_
Cuts at: 2568 5072 5282 2568
Size: 2504 210 4335
Fragments arranged by size:
4335 2504 210
SbfI CC_TGCA'GG
Cuts at: 251 1487 4096 251
Size: 1236 2609 3204
Fragments arranged by size:
3204 2609 1236
ScaI AGT'ACT
Cuts at: 6566 6566
Size: 7049
SexAI A'CCwGG_T
Cuts at: 3902 3902
Size: 7049
SfcI C'TryA_G
Cuts at: 33 247 1483 2605 2955 3260 4092 4777 5745
Size: 214 1236 1122 350 305 832 685 968
Cuts at: 5745 5936 33
Size: 191 1146
Fragments arranged by size:
1236 1146 1122 968 832 685 350 305
214 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1378 1699 3981 1378
Size: 321 2282 4446
Fragments arranged by size:
4446 2282 321
SfoI GGC'GCC
Cuts at: 1479 1613 2099 2362 2513 4481 4729 1479
Size: 134 486 263 151 1968 248 3799
Fragments arranged by size:
3799 1968 486 263 248 151 134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
SgrAI Cr'CCGG_yG
Cuts at: 1154 1154
Size: 7049
SmaI CCC'GGG
Cuts at: 1135 1915 2056 2398 4022 4071 1135
Size: 780 141 342 1624 49 4113
Fragments arranged by size:
4113 1624 780 342 141 49
SmlI C'TyrA_G
Cuts at: 128 1980 3531 4204 5119 5833 6095 128
Size: 1852 1551 673 915 714 262 1082
Fragments arranged by size:
1852 1551 1082 915 714 673 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7049
SphI G_CATG'C
Cuts at: 1459 4102 4602 1459
Size: 2643 500 3906
Fragments arranged by size:
3906 2643 500
SrfI GCCC'GGGC
Cuts at: 4022 4022
Size: 7049
StuI AGG'CCT
Cuts at: 817 974 817
Size: 157 6892
StyI C'CwwG_G
Cuts at: 107 635 1494 1694 2501 2612 2660 2807 2819
Size: 528 859 200 807 111 48 147 12
Cuts at: 2819 2840 2849 2942 2975 3020 3044 3812 3989
Size: 21 9 93 33 45 24 768 177
Cuts at: 3989 4183 4537 107
Size: 194 354 2619
Fragments arranged by size:
2619 859 807 768 528 354 200 194
177 147 111 93 48 45 33 24
21 12 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1192 1738 4632 4765 5224 5433 1192
Size: 546 2894 133 459 209 2808
Fragments arranged by size:
2894 2808 546 459 209 133
TatI w'GTAC_w
Cuts at: 3821 6564 3821
Size: 2743 4306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 180 212 2159 2191 6566 6598 180
Size: 32 1947 32 4375 32 631
Fragments arranged by size:
4375 1947 631 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4846 4846
Size: 7049
XbaI T'CTAG_A
Cuts at: 4080 4080
Size: 7049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3414 3868 4544 3414
Size: 454 676 5919
Fragments arranged by size:
5919 676 454
XhoI C'TCGA_G
Cuts at: 128 1980 128
Size: 1852 5197
XmaI C'CCGG_G
Cuts at: 1133 1913 2054 2396 4020 4069 1133
Size: 780 141 342 1624 49 4113
Fragments arranged by size:
4113 1624 780 342 141 49
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AleI AloI AscI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PsrI SnaBI SspI
SwaI XmnI ZraI