Restriction map for
pF1KF0040
(Circular) (Six-base) MAPSORT of: pF1KF0040.seq Check: 9199 from: 1 to: 6263
pF1KF0040 6263 bp
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 6263
AccI GT'mk_AC
Cuts at: 253 2747 3301 253
Size: 2494 554 3215
Fragments arranged by size:
3215 2494 554
Acc65I G'GTAC_C
Cuts at: 2793 3279 2793
Size: 486 5777
AclI AA'CG_TT
Cuts at: 6103 6103
Size: 6263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1104 1918 2419 2770 4088 4520 4873 5535
Size: 266 814 501 351 1318 432 353 662
Cuts at: 5535 838
Size: 1566
Fragments arranged by size:
1566 1318 814 662 501 432 353 351
266
AfeI AGC'GCT
Cuts at: 2017 2645 2017
Size: 628 5635
AflII C'TTAA_G
Cuts at: 1255 1255
Size: 6263
AflIII A'CryG_T
Cuts at: 3615 5415 5696 3615
Size: 1800 281 4182
Fragments arranged by size:
4182 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1767 1767
Size: 6263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1858 1890 2696 2728 1858
Size: 32 806 32 5393
Fragments arranged by size:
5393 806 32 32
AlwNI CAG_nnn'CTG
Cuts at: 675 2140 2721 3110 3568 5006 675
Size: 1465 581 389 458 1438 1932
Fragments arranged by size:
1932 1465 1438 581 458 389
ApaI G_GGCC'C
Cuts at: 3205 3205
Size: 6263
ApaLI G'TGCA_C
Cuts at: 5101 5101
Size: 6263
ApoI r'AATT_y
Cuts at: 764 1098 2233 2763 3267 5616 5872 764
Size: 334 1135 530 504 2349 256 1155
Fragments arranged by size:
2349 1155 1135 530 504 334 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
AvaI C'yCGr_G
Cuts at: 3283 3283
Size: 6263
BamHI G'GATC_C
Cuts at: 3288 3288
Size: 6263
BanI G'GyrC_C
Cuts at: 489 1564 2419 2793 3279 3693 3941 3976 489
Size: 1075 855 374 486 414 248 35 2776
Fragments arranged by size:
2776 1075 855 486 414 374 248 35
BanII G_rGCy'C
Cuts at: 3205 3277 4307 3205
Size: 72 1030 5161
Fragments arranged by size:
5161 1030 72
BbeI G_GCGC'C
Cuts at: 1568 3697 3945 1568
Size: 2129 248 3886
Fragments arranged by size:
3886 2129 248
BbsI GAAGACnn'nnnn_
Cuts at: 131 364 552 2667 131
Size: 233 188 2115 3727
Fragments arranged by size:
3727 2115 233 188
BciVI GTATCCnnnnn_n'
Cuts at: 1147 4161 5217 6263 1147
Size: 3014 1056 1046 1147
Fragments arranged by size:
3014 1147 1056 1046
BclI T'GATC_A
Cuts at: 3783 3783
Size: 6263
BglI GCCn_nnn'nGGC
Cuts at: 2336 2468 3033 3120 2336
Size: 132 565 87 5479
Fragments arranged by size:
5479 565 132 87
BglII A'GATC_T
Cuts at: 849 2148 3778 5815 849
Size: 1299 1630 2037 1297
Fragments arranged by size:
2037 1630 1299 1297
BlpI GC'TnA_GC
Cuts at: 923 3375 923
Size: 2452 3811
Bme1580I G_kGCm'C
Cuts at: 386 1499 2260 2271 3205 3888 3981 5105 386
Size: 1113 761 11 934 683 93 1124 1544
Fragments arranged by size:
1544 1124 1113 934 761 683 93 11
BmgBI CAC'GTC
Cuts at: 1764 2673 1764
Size: 909 5354
BmrI ACTGGGnnnn_n'
Cuts at: 1159 3475 3556 3891 6035 1159
Size: 2316 81 335 2144 1387
Fragments arranged by size:
2316 2144 1387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 701 3098 4630 701
Size: 2397 1532 2334
Fragments arranged by size:
2397 2334 1532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1039 1464 2215 649
Size: 390 425 751 4697
Fragments arranged by size:
4697 751 425 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1897 2064 3439 4318 5032 5330 1897
Size: 167 1375 879 714 298 2830
Fragments arranged by size:
2830 1375 879 714 298 167
BsaI GGTCTCn'nnnn_
Cuts at: 1015 2086 5967 1015
Size: 1071 3881 1311
Fragments arranged by size:
3881 1311 1071
BsaAI yAC'GTr
Cuts at: 789 3616 789
Size: 2827 3436
BsaBI GATnn'nnATC
Cuts at: 6 2147 3777 5814 6
Size: 2141 1630 2037 455
Fragments arranged by size:
2141 2037 1630 455
BsaHI Gr'CG_yC
Cuts at: 1565 1844 1906 3694 3942 4644 6140 1565
Size: 279 62 1788 248 702 1496 1688
Fragments arranged by size:
1788 1688 1496 702 279 248 62
BsaWI w'CCGG_w
Cuts at: 879 2563 3452 3973 4651 5062 5209 5483 879
Size: 1684 889 521 678 411 147 274 1659
Fragments arranged by size:
1684 1659 889 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 981 1011 1012 1042 1278 1308 2326 2356 2545
Size: 30 1 30 236 30 1018 30 189
Cuts at: 2545 2575 2593 2623 2696 2726 3024 3054 981
Size: 30 18 30 73 30 298 30 4190
Fragments arranged by size:
4190 1018 298 236 189 73 30 30
30 30 30 30 30 30 18 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1989 2052 2104 2200 2331 1989
Size: 63 52 96 131 5921
Fragments arranged by size:
5921 131 96 63 52
BseYI C'CCAG_C
Cuts at: 1829 2271 3688 4446 5111 5519 1829
Size: 442 1417 758 665 408 2573
Fragments arranged by size:
2573 1417 758 665 442 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 1000 1639 1699 1701 1794 2023 2240 2350
Size: 799 639 60 2 93 229 217 110
Cuts at: 2350 2632 201
Size: 282 3832
Fragments arranged by size:
3832 799 639 282 229 217 110 93
60 2
BsiEI CG_ry'CG
Cuts at: 104 2373 3191 3805 3851 5081 104
Size: 2269 818 614 46 1230 1286
Fragments arranged by size:
2269 1286 1230 818 614 46
BsiHKAI G_wGCw'C
Cuts at: 3277 4055 4245 5105 3277
Size: 778 190 860 4435
Fragments arranged by size:
4435 860 778 190
BsmI GAATG_Cn'
Cuts at: 607 648 2511 607
Size: 41 1863 4359
Fragments arranged by size:
4359 1863 41
BsmBI CGTCTCn'nnnn_
Cuts at: 1298 1443 1478 1897 1298
Size: 145 35 419 5664
Fragments arranged by size:
5664 419 145 35
Bsp1286I G_dGCh'C
Cuts at: 386 1499 2260 2271 3205 3277 3888 3981 4055
Size: 1113 761 11 934 72 611 93 74
Cuts at: 4055 4245 4307 5105 386
Size: 190 62 798 1544
Fragments arranged by size:
1544 1113 934 798 761 611 190 93
74 72 62 11
BspHI T'CATG_A
Cuts at: 759 4695 759
Size: 3936 2327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2407 3071 3313 3527 3829 4210 2407
Size: 664 242 214 302 381 4460
Fragments arranged by size:
4460 664 381 302 242 214
BsrBI CCG'CTC
Cuts at: 50 1605 2448 4555 4609 6093 6258 50
Size: 1555 843 2107 54 1484 165 55
Fragments arranged by size:
2107 1555 1484 843 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1319 1797 1817 1855 4175 1319
Size: 478 20 38 2320 3407
Fragments arranged by size:
3407 2320 478 38 20
BsrFI r'CCGG_y
Cuts at: 2074 4261 4442 2074
Size: 2187 181 3895
Fragments arranged by size:
3895 2187 181
BssSI C'ACGA_G
Cuts at: 902 1729 2575 2650 4534 5242 902
Size: 827 846 75 1884 708 1923
Fragments arranged by size:
1923 1884 846 827 708 75
BstAPI GCAn_nnn'nTGC
Cuts at: 3640 3640
Size: 6263
BstBI TT'CG_AA
Cuts at: 1172 2503 4624 1172
Size: 1331 2121 2811
Fragments arranged by size:
2811 2121 1331
BstEII G'GTnAC_C
Cuts at: 1330 1330
Size: 6263
BstXI CCAn_nnnn'nTGG
Cuts at: 202 2941 202
Size: 2739 3524
BstYI r'GATC_y
Cuts at: 1 498 849 1079 1883 2148 3079 3288 3755
Size: 497 351 230 804 265 931 209 467
Cuts at: 3755 3778 4113 4359 4665 4677 4763 5815 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 931 804 497 467 449 351 335
306 265 246 230 209 86 23 12
BtgI C'CryG_G
Cuts at: 107 201 797 1568 1590 2021 2700 107
Size: 94 596 771 22 431 679 3670
Fragments arranged by size:
3670 771 679 596 431 94 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 1433 4213 4394 4557 558
Size: 875 2780 181 163 2264
Fragments arranged by size:
2780 2264 875 181 163
BtsI GCAGTG_nn'
Cuts at: 746 2587 3506 746
Size: 1841 919 3503
Fragments arranged by size:
3503 1841 919
DraI TTT'AAA
Cuts at: 783 3262 783
Size: 2479 3784
DrdI GACnn_nn'nnGTC
Cuts at: 2440 3969 5313 2440
Size: 1529 1344 3390
Fragments arranged by size:
3390 1529 1344
EaeI y'GGCC_r
Cuts at: 1725 2370 2568 3112 3848 4022 4413 4440 5785
Size: 645 198 544 736 174 391 27 1345
Cuts at: 5785 1725
Size: 2203
Fragments arranged by size:
2203 1345 736 645 544 391 198 174
27
EagI C'GGCC_G
Cuts at: 2370 3848 2370
Size: 1478 4785
EarI CTCTTCn'nnn_
Cuts at: 287 416 473 2275 4286 4496 287
Size: 129 57 1802 2011 210 2054
Fragments arranged by size:
2054 2011 1802 210 129 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2392 4471 5213 5359 6073 2377
Size: 15 2079 742 146 714 2567
Fragments arranged by size:
2567 2079 742 714 146 15
EcoICRI GAG'CTC
Cuts at: 3275 3275
Size: 6263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 838 1039 1104 1464 1918 2215 2419 2770
Size: 189 201 65 360 454 297 204 351
Cuts at: 2770 4088 4520 4873 5535 649
Size: 1318 432 353 662 1377
Fragments arranged by size:
1377 1318 662 454 432 360 353 351
297 204 201 189 65
EcoNI CCTnn'n_nnAGG
Cuts at: 1971 1971
Size: 6263
EcoO109I rG'GnC_Cy
Cuts at: 1995 2190 2526 2606 3201 3402 1995
Size: 195 336 80 595 201 4856
Fragments arranged by size:
4856 595 336 201 195 80
EcoRI G'AATT_C
Cuts at: 2233 2763 3267 2233
Size: 530 504 5229
Fragments arranged by size:
5229 530 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 2691 4282 342
Size: 2349 1591 2323
Fragments arranged by size:
2349 2323 1591
FspI TGC'GCA
Cuts at: 4044 4044
Size: 6263
HaeII r_GCGC'y
Cuts at: 1568 2019 2647 3697 3945 5175 5946 1568
Size: 451 628 1050 248 1230 771 1885
Fragments arranged by size:
1885 1230 1050 771 628 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 606 638 1030 1062 1278 1310 1753 1785 2326
Size: 32 392 32 216 32 443 32 541
Cuts at: 2326 2358 4105 4135 4137 4167 606
Size: 32 1747 30 2 30 2702
Fragments arranged by size:
2702 1747 541 443 392 216 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3302 3302
Size: 6263
HindIII A'AGCT_T
Cuts at: 955 955
Size: 6263
KasI G'GCGC_C
Cuts at: 1564 3693 3941 1564
Size: 2129 248 3886
Fragments arranged by size:
3886 2129 248
KpnI G_GTAC'C
Cuts at: 2797 3283 2797
Size: 486 5777
MluI A'CGCG_T
Cuts at: 5696 5696
Size: 6263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2153 5022 5206 2153
Size: 2869 184 3210
Fragments arranged by size:
3210 2869 184
MscI TGG'CCA
Cuts at: 2570 3114 4024 2570
Size: 544 910 4809
Fragments arranged by size:
4809 910 544
MslI CAynn'nnrTG
Cuts at: 4252 4379 4252
Size: 127 6136
MspA1I CmG'CkG
Cuts at: 927 1829 2349 2941 3374 3688 4048 4832 5077
Size: 902 520 592 433 314 360 784 245
Cuts at: 5077 927
Size: 2113
Fragments arranged by size:
2113 902 784 592 520 433 360 314
245
NaeI GCC'GGC
Cuts at: 2076 4444 2076
Size: 2368 3895
NarI GG'CG_CC
Cuts at: 1565 3694 3942 1565
Size: 2129 248 3886
Fragments arranged by size:
3886 2129 248
NcoI C'CATG_G
Cuts at: 107 201 797 1568 1590 107
Size: 94 596 771 22 4780
Fragments arranged by size:
4780 771 596 94 22
NgoMIV G'CCGG_C
Cuts at: 2074 4442 2074
Size: 2368 3895
NspI r_CATG'y
Cuts at: 2356 3316 3816 5419 2356
Size: 960 500 1603 3200
Fragments arranged by size:
3200 1603 960 500
PasI CC'CwG_GG
Cuts at: 3198 3198
Size: 6263
PciI A'CATG_T
Cuts at: 5415 5415
Size: 6263
PflMI CCAn_nnn'nTGG
Cuts at: 456 456
Size: 6263
PmeI GTTT'AAAC
Cuts at: 3262 3262
Size: 6263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 488 562 594 2696 2728 4709 4741 456
Size: 32 74 32 2102 32 1981 32 1978
Fragments arranged by size:
2102 1981 1978 74 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2190 2526 2190
Size: 336 5927
PspOMI G'GGCC_C
Cuts at: 3201 3201
Size: 6263
PstI C_TGCA'G
Cuts at: 1865 3310 3995 1865
Size: 1445 685 4133
Fragments arranged by size:
4133 1445 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6263
PvuII CAG'CTG
Cuts at: 927 2941 3688 4048 927
Size: 2014 747 360 3142
Fragments arranged by size:
3142 2014 747 360
RsrII CG'GwC_CG
Cuts at: 3254 4458 3254
Size: 1204 5059
SacI G_AGCT'C
Cuts at: 3277 3277
Size: 6263
SalI G'TCGA_C
Cuts at: 3300 3300
Size: 6263
SapI GCTCTTCn'nnn_
Cuts at: 287 473 4286 4496 287
Size: 186 3813 210 2054
Fragments arranged by size:
3813 2054 210 186
SbfI CC_TGCA'GG
Cuts at: 3310 3310
Size: 6263
ScaI AGT'ACT
Cuts at: 5780 5780
Size: 6263
SfcI C'TryA_G
Cuts at: 33 1861 2106 2596 2744 3306 3991 4959 5150
Size: 1828 245 490 148 562 685 968 191
Cuts at: 5150 33
Size: 1146
Fragments arranged by size:
1828 1146 968 685 562 490 245 191
148
SfiI GGCCn_nnn'nGGCC
Cuts at: 3120 3120
Size: 6263
SfoI GGC'GCC
Cuts at: 1566 3695 3943 1566
Size: 2129 248 3886
Fragments arranged by size:
3886 2129 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
SmaI CCC'GGG
Cuts at: 3285 3285
Size: 6263
SmlI C'TyrA_G
Cuts at: 1255 1912 2079 3418 4333 5047 5309 1255
Size: 657 167 1339 915 714 262 2209
Fragments arranged by size:
2209 1339 915 714 657 262 167
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6263
SphI G_CATG'C
Cuts at: 3316 3816 3316
Size: 500 5763
StuI AGG'CCT
Cuts at: 3123 3123
Size: 6263
StyI C'CwwG_G
Cuts at: 107 201 797 1559 1568 1590 1839 3397 3751
Size: 94 596 762 9 22 249 1558 354
Cuts at: 3751 107
Size: 2619
Fragments arranged by size:
2619 1558 762 596 354 249 94 22
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3846 3979 4438 4647 3846
Size: 133 459 209 5462
Fragments arranged by size:
5462 459 209 133
TatI w'GTAC_w
Cuts at: 5778 5778
Size: 6263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1012 1044 2593 2625 3024 3056 5780 5812 1012
Size: 32 1549 32 399 32 2724 32 1463
Fragments arranged by size:
2724 1549 1463 399 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1290 2212 4060 1290
Size: 922 1848 3493
Fragments arranged by size:
3493 1848 922
XbaI T'CTAG_A
Cuts at: 2151 3294 2151
Size: 1143 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 915 1566 3758 915
Size: 651 2192 3420
Fragments arranged by size:
3420 2192 651
XmaI C'CCGG_G
Cuts at: 3283 3283
Size: 6263
XmnI GAAnn'nnTTC
Cuts at: 1190 2783 2961 1190
Size: 1593 178 4492
Fragments arranged by size:
4492 1593 178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AleI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BspEI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI