Restriction map for
pF1KF0052
(Circular) (Six-base) MAPSORT of: pF1KF0052.seq Check: 7918 from: 1 to: 4097
pF1KF0052 4097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 911 1113 911
Size: 202 3895
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1922 2354 3101 3369 1036
Size: 886 432 747 268 1764
Fragments arranged by size:
1764 886 747 432 268
AflII C'TTAA_G
Cuts at: 784 784
Size: 4097
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 848 848
Size: 4097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 440 472 440
Size: 32 4065
AlwNI CAG_nnn'CTG
Cuts at: 960 1090 1402 2969 960
Size: 130 312 1567 2088
Fragments arranged by size:
2088 1567 312 130
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 520 1101 3450 3706 520
Size: 581 2349 256 911
Fragments arranged by size:
2349 911 581 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 166 178 1117 166
Size: 12 939 3146
Fragments arranged by size:
3146 939 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 307 340 307
Size: 33 4064
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 911 1113 1527 1775 1810 911
Size: 202 414 248 35 3198
Fragments arranged by size:
3198 414 248 202 35
BanII G_rGCy'C
Cuts at: 1064 1111 2141 1064
Size: 47 1030 3020
Fragments arranged by size:
3020 1030 47
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 254 302 533 1015 254
Size: 48 231 482 3336
Fragments arranged by size:
3336 482 231 48
BciVI GTATCCnnnnn_n'
Cuts at: 428 1995 2756 4097 428
Size: 1567 761 1341 428
Fragments arranged by size:
1567 1341 761 428
BclI T'GATC_A
Cuts at: 971 1617 971
Size: 646 3451
BglII A'GATC_T
Cuts at: 995 1612 3649 995
Size: 617 2037 1443
Fragments arranged by size:
2037 1443 617
BlpI GC'TnA_GC
Cuts at: 563 1083 1209 563
Size: 520 126 3451
Fragments arranged by size:
3451 520 126
Bme1580I G_kGCm'C
Cuts at: 1722 1815 2871 1722
Size: 93 1056 2948
Fragments arranged by size:
2948 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 217 442 641 701 836 958 170
Size: 47 225 199 60 135 122 3309
Fragments arranged by size:
3309 225 199 135 122 60 47
Bpu10I CC'TnA_GC
Cuts at: 821 821
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1273 2152 2644 2942 331
Size: 942 879 492 298 1486
Fragments arranged by size:
1486 942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 792 3801 792
Size: 3009 1088
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1033 1611 3648 6
Size: 1027 578 2037 455
Fragments arranged by size:
2037 1027 578 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 440 470 440
Size: 30 4067
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 717 196
Size: 521 3576
BseYI C'CCAG_C
Cuts at: 722 1522 2280 2857 3353 722
Size: 800 758 577 496 1466
Fragments arranged by size:
1466 800 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 827 1111 1889 2079 2871 827
Size: 284 778 190 792 2053
Fragments arranged by size:
2053 792 778 284 190
BsmI GAATG_Cn'
Cuts at: 447 447
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 827 1064 1111 1722 1815 1889 2079 2141 2871
Size: 237 47 611 93 74 190 62 730
Cuts at: 2871 827
Size: 2053
Fragments arranged by size:
2053 730 611 237 190 93 74 62
47
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 647 3273 647
Size: 2626 1471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 1147 1361 1663 2044 720
Size: 427 214 302 381 2773
Fragments arranged by size:
2773 427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 159 1076 2095 2276 2485 159
Size: 917 1019 181 209 1771
Fragments arranged by size:
1771 1019 917 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 366 1474 366
Size: 1108 2989
BstBI TT'CG_AA
Cuts at: 866 2458 866
Size: 1592 2505
BstXI CCAn_nnnn'nTGG
Cuts at: 936 936
Size: 4097
BstYI r'GATC_y
Cuts at: 1 762 995 1034 1122 1589 1612 1947 2193
Size: 761 233 39 88 467 23 335 246
Cuts at: 2193 3205 3291 3303 3649 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 761 467 449 346 335 246 233
88 86 39 23 12
BtgI C'CryG_G
Cuts at: 116 842 116
Size: 726 3371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 518 1340 518
Size: 822 3275
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 1682 1856 2247 2274 3619 1682
Size: 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 185 391 457 1069 2120 2330 185
Size: 206 66 612 1051 210 1952
Fragments arranged by size:
1952 1051 612 210 206 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1063 2305 2615 2761 3907 1063
Size: 1242 310 146 1146 1253
Fragments arranged by size:
1253 1242 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 217 442 641 701 836 958 1036 1922
Size: 47 225 199 60 135 122 78 886
Cuts at: 1922 2354 3101 3369 170
Size: 432 747 268 898
Fragments arranged by size:
898 886 747 432 268 225 199 135
122 78 60 47
EcoO109I rG'GnC_Cy
Cuts at: 1236 1236
Size: 4097
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 157 206 1083 1531 1779 2801 3780 157
Size: 49 877 448 248 1022 979 474
Fragments arranged by size:
1022 979 877 474 448 248 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1939 1969 1971 2001 1939
Size: 30 2 30 4035
Fragments arranged by size:
4035 30 30 2
HincII GTy'rAC
Cuts at: 740 1136 740
Size: 396 3701
HindIII A'AGCT_T
Cuts at: 781 781
Size: 4097
HpaI GTT'AAC
Cuts at: 740 740
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 915 1117 915
Size: 202 3895
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 2768 2952 221
Size: 2547 184 1366
Fragments arranged by size:
2547 1366 184
MscI TGG'CCA
Cuts at: 1858 1858
Size: 4097
MslI CAynn'nnrTG
Cuts at: 1027 2086 2213 1027
Size: 1059 127 2911
Fragments arranged by size:
2911 1059 127
MspA1I CmG'CkG
Cuts at: 118 224 462 844 1087 1208 1522 1882 2895
Size: 106 238 382 243 121 314 360 1013
Cuts at: 2895 3140 118
Size: 245 1075
Fragments arranged by size:
1075 1013 382 360 314 245 243 238
121 106
NaeI GCC'GGC
Cuts at: 161 1078 2278 161
Size: 917 1200 1980
Fragments arranged by size:
1980 1200 917
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NgoMIV G'CCGG_C
Cuts at: 159 1076 2276 159
Size: 917 1200 1980
Fragments arranged by size:
1980 1200 917
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 440 472 3236 3268 440
Size: 32 2764 32 1269
Fragments arranged by size:
2764 1269 32 32
PstI C_TGCA'G
Cuts at: 1144 1829 1144
Size: 685 3412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 224 462 1087 1522 1882 224
Size: 238 625 435 360 2439
Fragments arranged by size:
2439 625 435 360 238
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SacII CC_GC'GG
Cuts at: 119 845 119
Size: 726 3371
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 185 1069 2120 2330 185
Size: 884 1051 210 1952
Fragments arranged by size:
1952 1051 884 210
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 172 3614 172
Size: 3442 655
SfcI C'TryA_G
Cuts at: 33 980 1140 1825 2818 3009 33
Size: 947 160 685 993 191 1121
Fragments arranged by size:
1121 993 947 685 191 160
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SgrAI Cr'CCGG_yG
Cuts at: 1076 1076
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 346 784 1252 2167 2659 2921 346
Size: 438 468 915 492 262 1522
Fragments arranged by size:
1522 915 492 468 438 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 1231 1585 1231
Size: 354 3743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 807 990 1680 1813 2272 2481 807
Size: 183 690 133 459 209 2423
Fragments arranged by size:
2423 690 459 209 183 133
TatI w'GTAC_w
Cuts at: 170 422 893 3612 170
Size: 252 471 2719 655
Fragments arranged by size:
2719 655 471 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 999 1031 3614 3646 999
Size: 32 2583 32 1450
Fragments arranged by size:
2583 1450 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI