Restriction map for
pF1KF0068
(Circular) (Six-base) MAPSORT of: pF1KF0068.seq Check: 8197 from: 1 to: 6686
pF1KF0068 6686 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1245 1509 2115 3188 1245
Size: 264 606 1073 4743
Fragments arranged by size:
4743 1073 606 264
AccI GT'mk_AC
Cuts at: 3724 3724
Size: 6686
Acc65I G'GTAC_C
Cuts at: 3702 3702
Size: 6686
AclI AA'CG_TT
Cuts at: 6526 6526
Size: 6686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 994 1339 1776 2068 2955 3337 4511 4943
Size: 246 345 437 292 887 382 1174 432
Cuts at: 4943 5296 5958 748
Size: 353 662 1476
Fragments arranged by size:
1476 1174 887 662 437 432 382 353
345 292 246
AfeI AGC'GCT
Cuts at: 3022 3022
Size: 6686
AflIII A'CryG_T
Cuts at: 4038 5838 6119 4038
Size: 1800 281 4605
Fragments arranged by size:
4605 1800 281
AgeI A'CCGG_T
Cuts at: 2058 2058
Size: 6686
AleI CACnn'nnGTG
Cuts at: 3121 3121
Size: 6686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 2763 532
Size: 2231 4455
AlwNI CAG_nnn'CTG
Cuts at: 843 951 1023 1599 1653 1761 1878 2227 2736
Size: 108 72 576 54 108 117 349 509
Cuts at: 2736 3629 3648 3991 5429 843
Size: 893 19 343 1438 2100
Fragments arranged by size:
2100 1438 893 576 509 349 343 117
108 108 72 54 19
ApaI G_GGCC'C
Cuts at: 2758 2993 2758
Size: 235 6451
ApaLI G'TGCA_C
Cuts at: 2800 5524 2800
Size: 2724 3962
ApoI r'AATT_y
Cuts at: 313 872 1726 3690 6039 6295 313
Size: 559 854 1964 2349 256 704
Fragments arranged by size:
2349 1964 854 704 559 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6686
AvaI C'yCGr_G
Cuts at: 707 1478 1857 2386 3338 3706 707
Size: 771 379 529 952 368 3687
Fragments arranged by size:
3687 952 771 529 379 368
BamHI G'GATC_C
Cuts at: 616 3711 616
Size: 3095 3591
BanI G'GyrC_C
Cuts at: 1751 1898 2000 2236 2513 2921 3702 4116 4364
Size: 147 102 236 277 408 781 414 248
Cuts at: 4364 4399 1751
Size: 35 4038
Fragments arranged by size:
4038 781 414 408 277 248 236 147
102 35
BanII G_rGCy'C
Cuts at: 535 908 1351 1556 1716 1941 1960 2358 2758
Size: 373 443 205 160 225 19 398 400
Cuts at: 2758 2771 2993 3700 4730 535
Size: 13 222 707 1030 2491
Fragments arranged by size:
2491 1030 707 443 400 398 373 225
222 205 160 19 13
BbeI G_GCGC'C
Cuts at: 2925 4120 4368 2925
Size: 1195 248 5243
Fragments arranged by size:
5243 1195 248
BbvCI CC'TCA_GC
Cuts at: 445 1814 3530 3566 445
Size: 1369 1716 36 3565
Fragments arranged by size:
3565 1716 1369 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 323 357 323
Size: 34 6652
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4584 5640 6686 3506
Size: 1078 1056 1046 3506
Fragments arranged by size:
3506 1078 1056 1046
BclI T'GATC_A
Cuts at: 4206 4206
Size: 6686
BglI GCCn_nnn'nGGC
Cuts at: 934 990 1298 934
Size: 56 308 6322
Fragments arranged by size:
6322 308 56
BglII A'GATC_T
Cuts at: 3086 3578 4201 6238 3086
Size: 492 623 2037 3534
Fragments arranged by size:
3534 2037 623 492
BlpI GC'TnA_GC
Cuts at: 907 3798 907
Size: 2891 3795
Bme1580I G_kGCm'C
Cuts at: 364 747 1155 1193 1695 2241 2411 2436 2627
Size: 383 408 38 502 546 170 25 191
Cuts at: 2627 2758 2804 2993 4311 4404 5528 364
Size: 131 46 189 1318 93 1124 1522
Fragments arranged by size:
1522 1318 1124 546 502 408 383 191
189 170 131 93 46 38 25
BmgBI CAC'GTC
Cuts at: 1808 1808
Size: 6686
BmrI ACTGGGnnnn_n'
Cuts at: 605 853 2665 2927 3898 3979 4314 6458 605
Size: 248 1812 262 971 81 335 2144 833
Fragments arranged by size:
2144 1812 971 833 335 262 248 81
BmtI G_CTAG'C
Cuts at: 2078 2078
Size: 6686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5053 5053
Size: 6686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 189 428 619 1021 1144 1492 1558 1864
Size: 47 239 191 402 123 348 66 306
Cuts at: 1864 2249 2473 2518 3120 3428 3544 3594 3682
Size: 385 224 45 602 308 116 50 88
Cuts at: 3682 142
Size: 3146
Fragments arranged by size:
3146 602 402 385 348 308 306 239
224 191 123 116 88 66 50 47
45
Bpu10I CC'TnA_GC
Cuts at: 445 629 1345 1814 3530 3566 445
Size: 184 716 469 1716 36 3565
Fragments arranged by size:
3565 1716 716 469 184 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 2056 2854 3862 4741 5455 5753 549
Size: 1507 798 1008 879 714 298 1482
Fragments arranged by size:
1507 1482 1008 879 798 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 563 3052 3404 3455 3484 6390 563
Size: 2489 352 51 29 2906 859
Fragments arranged by size:
2906 2489 859 352 51 29
BsaAI yAC'GTr
Cuts at: 4039 4039
Size: 6686
BsaBI GATnn'nnATC
Cuts at: 6 2147 4200 6237 6
Size: 2141 2053 2037 455
Fragments arranged by size:
2141 2053 2037 455
BsaHI Gr'CG_yC
Cuts at: 2843 2922 3343 4117 4365 5067 6563 2843
Size: 79 421 774 248 702 1496 2966
Fragments arranged by size:
2966 1496 774 702 421 248 79
BsaWI w'CCGG_w
Cuts at: 2058 3875 4396 5074 5485 5632 5906 2058
Size: 1817 521 678 411 147 274 2838
Fragments arranged by size:
2838 1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2250 2280 2329 2359 2662 2692 3007 3037 2250
Size: 30 49 30 303 30 315 30 5899
Fragments arranged by size:
5899 315 303 49 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 298 366 1082 1555 1831 2246 2971 223
Size: 75 68 716 473 276 415 725 3938
Fragments arranged by size:
3938 725 716 473 415 276 75 68
BseYI C'CCAG_C
Cuts at: 327 753 1193 1546 1661 1715 1745 1953 1985
Size: 426 440 353 115 54 30 208 32
Cuts at: 1985 3274 3465 3499 3540 4111 4869 5534 5942
Size: 1289 191 34 41 571 758 665 408
Cuts at: 5942 327
Size: 1071
Fragments arranged by size:
1289 1071 758 665 571 440 426 408
353 208 191 115 54 41 34 32
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 795 1396 1477 1870 2087 2575 3433 204
Size: 591 601 81 393 217 488 858 3457
Fragments arranged by size:
3457 858 601 591 488 393 217 81
BsiEI CG_ry'CG
Cuts at: 104 2145 3297 4228 4274 5504 104
Size: 2041 1152 931 46 1230 1286
Fragments arranged by size:
2041 1286 1230 1152 931 46
BsiHKAI G_wGCw'C
Cuts at: 882 2771 2804 3700 4478 4668 5528 882
Size: 1889 33 896 778 190 860 2040
Fragments arranged by size:
2040 1889 896 860 778 190 33
Bsp1286I G_dGCh'C
Cuts at: 364 535 747 882 908 1155 1193 1351 1556
Size: 171 212 135 26 247 38 158 205
Cuts at: 1556 1695 1716 1941 1960 2241 2358 2411 2436
Size: 139 21 225 19 281 117 53 25
Cuts at: 2436 2627 2758 2771 2804 2993 3700 4311 4404
Size: 191 131 13 33 189 707 611 93
Cuts at: 4404 4478 4668 4730 5528 364
Size: 74 190 62 798 1522
Fragments arranged by size:
1522 798 707 611 281 247 225 212
205 191 190 189 171 158 139 135
131 117 93 74 62 53 38 33
26 25 21 19 13
BspHI T'CATG_A
Cuts at: 5118 5118
Size: 6686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 225 818 1245 1509 1611 2115 2181 2693 2837
Size: 593 427 264 102 504 66 512 144
Cuts at: 2837 3083 3188 3455 3736 3950 4252 4633 225
Size: 246 105 267 281 214 302 381 2278
Fragments arranged by size:
2278 593 512 504 427 381 302 281
267 264 246 214 144 105 102 66
BsrBI CCG'CTC
Cuts at: 50 2340 4978 5032 6516 6681 50
Size: 2290 2638 54 1484 165 55
Fragments arranged by size:
2638 2290 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1373 3266 3347 4598 1373
Size: 1893 81 1251 3461
Fragments arranged by size:
3461 1893 1251 81
BsrFI r'CCGG_y
Cuts at: 2058 4684 4865 2058
Size: 2626 181 3879
Fragments arranged by size:
3879 2626 181
BsrGI T'GTAC_A
Cuts at: 3455 3455
Size: 6686
BssSI C'ACGA_G
Cuts at: 2522 4957 5665 2522
Size: 2435 708 3543
Fragments arranged by size:
3543 2435 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1257 1878 2889 4063 1150
Size: 107 621 1011 1174 3773
Fragments arranged by size:
3773 1174 1011 621 107
BstBI TT'CG_AA
Cuts at: 5047 5047
Size: 6686
BstEII G'GTnAC_C
Cuts at: 1338 1338
Size: 6686
BstXI CCAn_nnnn'nTGG
Cuts at: 687 1953 2443 3243 687
Size: 1266 490 800 4130
Fragments arranged by size:
4130 1266 800 490
BstYI r'GATC_y
Cuts at: 1 616 3086 3578 3711 4178 4201 4536 4782
Size: 615 2470 492 133 467 23 335 246
Cuts at: 4782 5088 5100 5186 6238 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2470 1052 615 492 467 449 335 306
246 133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2122 2122
Size: 6686
BtgI C'CryG_G
Cuts at: 107 1199 1270 1946 2333 2421 2837 2985 3236
Size: 1092 71 676 387 88 416 148 251
Cuts at: 3236 107
Size: 3557
Fragments arranged by size:
3557 1092 676 416 387 251 148 88
71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 529 4636 4817 4980 529
Size: 4107 181 163 2235
Fragments arranged by size:
4107 2235 181 163
BtsI GCAGTG_nn'
Cuts at: 734 2181 2472 2719 3929 734
Size: 1447 291 247 1210 3491
Fragments arranged by size:
3491 1447 1210 291 247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2982 3017 3166 3201 2982
Size: 35 149 35 6467
Fragments arranged by size:
6467 149 35 35
DraI TTT'AAA
Cuts at: 3685 3685
Size: 6686
DraIII CAC_nnn'GTG
Cuts at: 460 3056 460
Size: 2596 4090
DrdI GACnn_nn'nnGTC
Cuts at: 3301 4392 5736 3301
Size: 1091 1344 4251
Fragments arranged by size:
4251 1344 1091
EaeI y'GGCC_r
Cuts at: 677 689 1528 1943 2195 2330 2834 3546 4271
Size: 12 839 415 252 135 504 712 725
Cuts at: 4271 4445 4836 4863 6208 677
Size: 174 391 27 1345 1155
Fragments arranged by size:
1345 1155 839 725 712 504 415 391
252 174 135 27 12
EagI C'GGCC_G
Cuts at: 4271 4271
Size: 6686
EarI CTCTTCn'nnn_
Cuts at: 200 901 1795 2974 3476 4709 4919 200
Size: 701 894 1179 502 1233 210 1967
Fragments arranged by size:
1967 1233 1179 894 701 502 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 784 1780 1960 3085 4894 5636 5782 6496 784
Size: 996 180 1125 1809 742 146 714 974
Fragments arranged by size:
1809 1125 996 974 742 714 180 146
EcoICRI GAG'CTC
Cuts at: 2769 3698 2769
Size: 929 5757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 189 428 619 748 994 1021 1144 1339
Size: 47 239 191 129 246 27 123 195
Cuts at: 1339 1492 1558 1776 1864 2068 2249 2473 2518
Size: 153 66 218 88 204 181 224 45
Cuts at: 2518 2955 3120 3337 3428 3544 3594 3682 4511
Size: 437 165 217 91 116 50 88 829
Cuts at: 4511 4943 5296 5958 142
Size: 432 353 662 870
Fragments arranged by size:
870 829 662 437 432 353 246 239
224 218 217 204 195 191 181 165
153 129 123 116 91 88 88 66
50 47 45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 306 1159 2125 2245 2270 2953 306
Size: 853 966 120 25 683 4039
Fragments arranged by size:
4039 966 853 683 120 25
EcoO109I rG'GnC_Cy
Cuts at: 116 240 247 400 495 1242 1329 1449 2094
Size: 124 7 153 95 747 87 120 645
Cuts at: 2094 2415 2754 2755 3389 3437 3645 3657 3825
Size: 321 339 1 634 48 208 12 168
Cuts at: 3825 116
Size: 2977
Fragments arranged by size:
2977 747 645 634 339 321 208 168
153 124 120 95 87 48 12 7
1
EcoRI G'AATT_C
Cuts at: 1726 3690 1726
Size: 1964 4722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3042 4705 3042
Size: 1663 5023
FspI TGC'GCA
Cuts at: 2914 3217 4467 2914
Size: 303 1250 5133
Fragments arranged by size:
5133 1250 303
FspAI rTGC'GCAy
Cuts at: 2914 3217 2914
Size: 303 6383
HaeII r_GCGC'y
Cuts at: 2925 3024 4120 4368 5598 6369 2925
Size: 99 1096 248 1230 771 3242
Fragments arranged by size:
3242 1230 1096 771 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 608 640 1923 1955 2662 2694 3217
Size: 32 14 32 1283 32 707 32 523
Cuts at: 3217 3249 3403 3435 3568 3600 4528 4558 4560
Size: 32 154 32 133 32 928 30 2
Cuts at: 4560 4590 562
Size: 30 2658
Fragments arranged by size:
2658 1283 928 707 523 154 133 32
32 32 32 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 2865 3003 3306 3725 2865
Size: 138 303 419 5826
Fragments arranged by size:
5826 419 303 138
HindIII A'AGCT_T
Cuts at: 2032 2032
Size: 6686
HpaI GTT'AAC
Cuts at: 2865 2865
Size: 6686
KasI G'GCGC_C
Cuts at: 2921 4116 4364 2921
Size: 1195 248 5243
Fragments arranged by size:
5243 1195 248
KpnI G_GTAC'C
Cuts at: 3706 3706
Size: 6686
MluI A'CGCG_T
Cuts at: 6119 6119
Size: 6686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5445 5629 5445
Size: 184 6502
MscI TGG'CCA
Cuts at: 679 691 1530 1945 2332 2836 4447 679
Size: 12 839 415 387 504 1611 2918
Fragments arranged by size:
2918 1611 839 504 415 387 12
MslI CAynn'nnrTG
Cuts at: 3121 3241 4675 4802 3121
Size: 120 1434 127 5005
Fragments arranged by size:
5005 1434 127 120
MspA1I CmG'CkG
Cuts at: 331 999 1224 1653 1719 1749 1758 1878 1884
Size: 668 225 429 66 30 9 120 6
Cuts at: 1884 1985 2101 2310 2423 2811 2886 3166 3485
Size: 101 116 209 113 388 75 280 319
Cuts at: 3485 3629 3797 4111 4471 5255 5500 331
Size: 144 168 314 360 784 245 1517
Fragments arranged by size:
1517 784 668 429 388 360 319 314
280 245 225 209 168 144 120 116
113 101 75 66 30 9 6
NaeI GCC'GGC
Cuts at: 4867 4867
Size: 6686
NarI GG'CG_CC
Cuts at: 2922 4117 4365 2922
Size: 1195 248 5243
Fragments arranged by size:
5243 1195 248
NcoI C'CATG_G
Cuts at: 107 1199 1270 2837 2985 3236 107
Size: 1092 71 1567 148 251 3557
Fragments arranged by size:
3557 1567 1092 251 148 71
NgoMIV G'CCGG_C
Cuts at: 4865 4865
Size: 6686
NheI G'CTAG_C
Cuts at: 2074 2074
Size: 6686
NspI r_CATG'y
Cuts at: 1569 3739 4239 5842 1569
Size: 2170 500 1603 2413
Fragments arranged by size:
2413 2170 1603 500
PasI CC'CwG_GG
Cuts at: 475 535 931 1687 2412 3648 475
Size: 60 396 756 725 1236 3513
Fragments arranged by size:
3513 1236 756 725 396 60
PciI A'CATG_T
Cuts at: 5838 5838
Size: 6686
PflMI CCAn_nnn'nTGG
Cuts at: 167 1200 1470 1761 2227 2706 167
Size: 1033 270 291 466 479 4147
Fragments arranged by size:
4147 1033 479 466 291 270
PfoI T'CCnGG_A
Cuts at: 307 3405 307
Size: 3098 3588
PmeI GTTT'AAAC
Cuts at: 3685 3685
Size: 6686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3108 5132 5164 3076
Size: 32 2024 32 4598
Fragments arranged by size:
4598 2024 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 247 495 1242 1329 2094 3437 240
Size: 7 248 747 87 765 1343 3489
Fragments arranged by size:
3489 1343 765 747 248 87 7
PshAI GACnn'nnGTC
Cuts at: 245 500 1240 3044 245
Size: 255 740 1804 3887
Fragments arranged by size:
3887 1804 740 255
PspOMI G'GGCC_C
Cuts at: 2754 2989 2754
Size: 235 6451
PstI C_TGCA'G
Cuts at: 336 998 1430 1517 1649 1883 2732 3131 3733
Size: 662 432 87 132 234 849 399 602
Cuts at: 3733 4418 336
Size: 685 2604
Fragments arranged by size:
2604 849 685 662 602 432 399 234
132 87
PvuI CG_AT'CG
Cuts at: 104 2145 104
Size: 2041 4645
PvuII CAG'CTG
Cuts at: 331 999 1224 1719 1878 1884 2310 2811 2886
Size: 668 225 495 159 6 426 501 75
Cuts at: 2886 3166 3629 4111 4471 331
Size: 280 463 482 360 2546
Fragments arranged by size:
2546 668 501 495 482 463 426 360
280 225 159 75 6
RsrII CG'GwC_CG
Cuts at: 4881 4881
Size: 6686
SacI G_AGCT'C
Cuts at: 2771 3700 2771
Size: 929 5757
SacII CC_GC'GG
Cuts at: 2424 2424
Size: 6686
SalI G'TCGA_C
Cuts at: 3723 3723
Size: 6686
SanDI GG'GwC_CC
Cuts at: 495 3437 495
Size: 2942 3744
SapI GCTCTTCn'nnn_
Cuts at: 1795 2974 3476 4709 4919 1795
Size: 1179 502 1233 210 3562
Fragments arranged by size:
3562 1233 1179 502 210
SbfI CC_TGCA'GG
Cuts at: 3733 3733
Size: 6686
ScaI AGT'ACT
Cuts at: 6203 6203
Size: 6686
SexAI A'CCwGG_T
Cuts at: 944 1604 944
Size: 660 6026
SfcI C'TryA_G
Cuts at: 33 289 332 994 1129 1228 1356 1426 1513
Size: 256 43 662 135 99 128 70 87
Cuts at: 1513 1645 1879 2133 2305 2577 2728 3127 3729
Size: 132 234 254 172 272 151 399 602
Cuts at: 3729 4414 5382 5573 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 662 602 399 272 256
254 234 191 172 151 135 132 128
99 87 70 43
SfoI GGC'GCC
Cuts at: 2923 4118 4366 2923
Size: 1195 248 5243
Fragments arranged by size:
5243 1195 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6686
SgrAI Cr'CCGG_yG
Cuts at: 2058 2058
Size: 6686
SmaI CCC'GGG
Cuts at: 1480 1859 3708 1480
Size: 379 1849 4458
Fragments arranged by size:
4458 1849 379
SmlI C'TyrA_G
Cuts at: 528 2035 2869 3841 4756 5470 5732 528
Size: 1507 834 972 915 714 262 1482
Fragments arranged by size:
1507 1482 972 915 834 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6686
SphI G_CATG'C
Cuts at: 3739 4239 3739
Size: 500 6186
StuI AGG'CCT
Cuts at: 1073 1093 1801 2131 3133 3157 3256 1073
Size: 20 708 330 1002 24 99 4503
Fragments arranged by size:
4503 1002 708 330 99 24 20
StyI C'CwwG_G
Cuts at: 107 564 717 1199 1270 1581 2837 2985 3042
Size: 457 153 482 71 311 1256 148 57
Cuts at: 3042 3236 3820 4174 107
Size: 194 584 354 2619
Fragments arranged by size:
2619 1256 584 482 457 354 311 194
153 148 71 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 1207 2531 3229 4269 4402 4861 5070 289
Size: 918 1324 698 1040 133 459 209 1905
Fragments arranged by size:
1905 1324 1040 918 698 459 209 133
TatI w'GTAC_w
Cuts at: 834 2363 3455 6201 834
Size: 1529 1092 2746 1319
Fragments arranged by size:
2746 1529 1319 1092
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 839 871 6203 6235 839
Size: 32 5332 32 1290
Fragments arranged by size:
5332 1290 32 32
Tth111I GACn'n_nGTC
Cuts at: 4483 4483
Size: 6686
XbaI T'CTAG_A
Cuts at: 2358 3717 2358
Size: 1359 5327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 2703 4181 1277
Size: 1426 1478 3782
Fragments arranged by size:
3782 1478 1426
XmaI C'CCGG_G
Cuts at: 1478 1857 3706 1478
Size: 379 1849 4458
Fragments arranged by size:
4458 1849 379
XmnI GAAnn'nnTTC
Cuts at: 2380 2380
Size: 6686
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BfrBI BsiWI BsmI BsmBI BspEI BssHII BstZ17I ClaI
EcoRV FseI MfeI NdeI NotI NruI NsiI PacI
PmlI PsiI PspXI PsrI SfiI SnaBI SrfI SspI
SwaI XhoI ZraI