Restriction map for
pF1KF0120
(Circular) (Six-base) MAPSORT of: pF1KF0120.seq Check: 7785 from: 1 to: 6575
pF1KF0120 6575 bp
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AccI GT'mk_AC
Cuts at: 2564 2681 3613 2564
Size: 117 932 5526
Fragments arranged by size:
5526 932 117
Acc65I G'GTAC_C
Cuts at: 2378 3591 2378
Size: 1213 5362
AclI AA'CG_TT
Cuts at: 6415 6415
Size: 6575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 886 955 1580 1633 1675 2512 2791 3442
Size: 714 69 625 53 42 837 279 651
Cuts at: 3442 4400 4832 5185 5847 172
Size: 958 432 353 662 900
Fragments arranged by size:
958 900 837 714 662 651 625 432
353 279 69 53 42
AfeI AGC'GCT
Cuts at: 2738 2738
Size: 6575
AflIII A'CryG_T
Cuts at: 3203 3476 3927 5727 6008 3203
Size: 273 451 1800 281 3770
Fragments arranged by size:
3770 1800 451 281 273
AgeI A'CCGG_T
Cuts at: 809 809
Size: 6575
AhdI GACnn_n'nnGTC
Cuts at: 1517 1517
Size: 6575
AleI CACnn'nnGTG
Cuts at: 460 2071 2516 460
Size: 1611 445 4519
Fragments arranged by size:
4519 1611 445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1642 1642
Size: 6575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3385 3417 3385
Size: 32 6543
AlwNI CAG_nnn'CTG
Cuts at: 906 954 1062 1452 2244 2652 3081 3880 5318
Size: 48 108 390 792 408 429 799 1438
Cuts at: 5318 906
Size: 2163
Fragments arranged by size:
2163 1438 799 792 429 408 390 108
48
ApaI G_GGCC'C
Cuts at: 2092 2099 2092
Size: 7 6568
ApaLI G'TGCA_C
Cuts at: 2182 2509 5413 2182
Size: 327 2904 3344
Fragments arranged by size:
3344 2904 327
ApoI r'AATT_y
Cuts at: 394 3579 5928 6184 394
Size: 3185 2349 256 785
Fragments arranged by size:
3185 2349 785 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
AvaI C'yCGr_G
Cuts at: 787 1262 1809 2756 2874 3090 3185 3595 787
Size: 475 547 947 118 216 95 410 3767
Fragments arranged by size:
3767 947 547 475 410 216 118 95
BamHI G'GATC_C
Cuts at: 3600 3600
Size: 6575
BanI G'GyrC_C
Cuts at: 1415 2378 2596 2686 3097 3591 4005 4253 4288
Size: 963 218 90 411 494 414 248 35
Cuts at: 4288 1415
Size: 3702
Fragments arranged by size:
3702 963 494 414 411 248 218 90
35
BanII G_rGCy'C
Cuts at: 440 1154 1808 2092 2099 2622 3589 4619 440
Size: 714 654 284 7 523 967 1030 2396
Fragments arranged by size:
2396 1030 967 714 654 523 284 7
BbeI G_GCGC'C
Cuts at: 1419 4009 4257 1419
Size: 2590 248 3737
Fragments arranged by size:
3737 2590 248
BbsI GAAGACnn'nnnn_
Cuts at: 369 943 1568 1880 3169 369
Size: 574 625 312 1289 3775
Fragments arranged by size:
3775 1289 625 574 312
BbvCI CC'TCA_GC
Cuts at: 2980 2980
Size: 6575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1848 1882 90
Size: 34 1724 34 4783
Fragments arranged by size:
4783 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4473 5529 6575 4473
Size: 1056 1046 4473
Fragments arranged by size:
4473 1056 1046
BclI T'GATC_A
Cuts at: 2414 3299 4095 2414
Size: 885 796 4894
Fragments arranged by size:
4894 885 796
BfrBI ATG'CAT
Cuts at: 3215 3215
Size: 6575
BglI GCCn_nnn'nGGC
Cuts at: 482 1102 482
Size: 620 5955
BglII A'GATC_T
Cuts at: 1905 4090 6127 1905
Size: 2185 2037 2353
Fragments arranged by size:
2353 2185 2037
BlpI GC'TnA_GC
Cuts at: 205 3687 205
Size: 3482 3093
Bme1580I G_kGCm'C
Cuts at: 1688 2081 2092 2099 2186 2513 2599 2709 4200
Size: 393 11 7 87 327 86 110 1491
Cuts at: 4200 4293 5417 1688
Size: 93 1124 2846
Fragments arranged by size:
2846 1491 1124 393 327 110 93 87
86 11 7
BmgBI CAC'GTC
Cuts at: 2514 2514
Size: 6575
BmrI ACTGGGnnnn_n'
Cuts at: 437 3787 3868 4203 6347 437
Size: 3350 81 335 2144 665
Fragments arranged by size:
3350 2144 665 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4942 4942
Size: 6575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 862 934 1066 1150 1168 1243 1357 1420
Size: 258 72 132 84 18 75 114 63
Cuts at: 1420 2026 2445 3289 3457 604
Size: 606 419 844 168 3722
Fragments arranged by size:
3722 844 606 419 258 168 132 114
84 75 72 63 18
Bpu10I CC'TnA_GC
Cuts at: 2980 2980
Size: 6575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 3751 4630 5344 5642 1138
Size: 2613 879 714 298 2071
Fragments arranged by size:
2613 2071 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 770 3163 6279 770
Size: 2393 3116 1066
Fragments arranged by size:
3116 2393 1066
BsaAI yAC'GTr
Cuts at: 2082 3928 2082
Size: 1846 4729
BsaBI GATnn'nnATC
Cuts at: 6 1360 4089 6126 6
Size: 1354 2729 2037 455
Fragments arranged by size:
2729 2037 1354 455
BsaHI Gr'CG_yC
Cuts at: 167 1416 1706 1898 2474 2915 4006 4254 4956
Size: 1249 290 192 576 441 1091 248 702
Cuts at: 4956 6452 167
Size: 1496 290
Fragments arranged by size:
1496 1249 1091 702 576 441 290 290
248 192
BsaWI w'CCGG_w
Cuts at: 809 2381 2711 3764 4285 4963 5374 5521 5795
Size: 1572 330 1053 521 678 411 147 274
Cuts at: 5795 809
Size: 1589
Fragments arranged by size:
1589 1572 1053 678 521 411 330 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 943 973 1141 1171 1948 1978 3385 3415 943
Size: 30 168 30 777 30 1407 30 4103
Fragments arranged by size:
4103 1407 777 168 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 131 760 783 841 1105 1123 1147 1240 1354
Size: 629 23 58 264 18 24 93 114
Cuts at: 1354 1564 1938 1950 1953 1956 1959 2620 2689
Size: 210 374 12 3 3 3 661 69
Cuts at: 2689 3409 3412 3508 3544 131
Size: 720 3 96 36 3162
Fragments arranged by size:
3162 720 661 629 374 264 210 114
96 93 69 58 36 24 23 18
12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1595 2141 2154 2605 2621 2967 3274 4000 4758
Size: 546 13 451 16 346 307 726 758
Cuts at: 4758 5423 5831 1595
Size: 665 408 2339
Fragments arranged by size:
2339 758 726 665 546 451 408 346
307 16 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 2338 2355 2463 3094 3270 2125
Size: 213 17 108 631 176 5430
Fragments arranged by size:
5430 631 213 176 108 17
BsiEI CG_ry'CG
Cuts at: 104 1032 4117 4163 5393 104
Size: 928 3085 46 1230 1286
Fragments arranged by size:
3085 1286 1230 928 46
BsiHKAI G_wGCw'C
Cuts at: 1154 1162 2186 2513 3041 3329 3589 4367 4557
Size: 8 1024 327 528 288 260 778 190
Cuts at: 4557 5417 1154
Size: 860 2312
Fragments arranged by size:
2312 1024 860 778 528 327 288 260
190 8
BsmI GAATG_Cn'
Cuts at: 3217 3217
Size: 6575
BsmBI CGTCTCn'nnnn_
Cuts at: 139 2776 3382 139
Size: 2637 606 3332
Fragments arranged by size:
3332 2637 606
Bsp1286I G_dGCh'C
Cuts at: 440 1154 1162 1688 1808 2081 2092 2099 2186
Size: 714 8 526 120 273 11 7 87
Cuts at: 2186 2513 2599 2622 2709 3041 3329 3589 4200
Size: 327 86 23 87 332 288 260 611
Cuts at: 4200 4293 4367 4557 4619 5417 440
Size: 93 74 190 62 798 1598
Fragments arranged by size:
1598 798 714 611 526 332 327 288
273 260 190 120 93 87 87 86
74 62 23 11 8 7
BspEI T'CCGG_A
Cuts at: 2711 2711
Size: 6575
BspHI T'CATG_A
Cuts at: 764 5007 764
Size: 4243 2332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 554 891 1091 1472 2001 2810 3625 3839
Size: 303 337 200 381 529 809 815 214
Cuts at: 3839 4141 4522 251
Size: 302 381 2304
Fragments arranged by size:
2304 815 809 529 381 381 337 303
302 214 200
BsrBI CCG'CTC
Cuts at: 50 642 666 918 1236 1242 2859 4867 4921
Size: 592 24 252 318 6 1617 2008 54
Cuts at: 4921 6405 6570 50
Size: 1484 165 55
Fragments arranged by size:
2008 1617 1484 592 318 252 165 55
54 24 6
BsrDI GCAATG_nn'
Cuts at: 4487 4487
Size: 6575
BsrFI r'CCGG_y
Cuts at: 194 329 809 2218 3029 3416 4573 4754 194
Size: 135 480 1409 811 387 1157 181 2015
Fragments arranged by size:
2015 1409 1157 811 480 387 181 135
BsrGI T'GTAC_A
Cuts at: 413 1187 413
Size: 774 5801
BssHII G'CGCG_C
Cuts at: 2555 2555
Size: 6575
BssSI C'ACGA_G
Cuts at: 478 1687 2542 4846 5554 478
Size: 1209 855 2304 708 1499
Fragments arranged by size:
2304 1499 1209 855 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3081 3264 3952 3081
Size: 183 688 5704
Fragments arranged by size:
5704 688 183
BstBI TT'CG_AA
Cuts at: 3209 4936 3209
Size: 1727 4848
BstEII G'GTnAC_C
Cuts at: 2064 2064
Size: 6575
BstXI CCAn_nnnn'nTGG
Cuts at: 462 465 2469 3057 3069 3552 462
Size: 3 2004 588 12 483 3485
Fragments arranged by size:
3485 2004 588 483 12 3
BstYI r'GATC_y
Cuts at: 1 1073 1325 1726 1905 3600 4067 4090 4425
Size: 1072 252 401 179 1695 467 23 335
Cuts at: 4425 4671 4977 4989 5075 6127 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1695 1072 1052 467 449 401 335 306
252 246 179 86 23 12
BtgI C'CryG_G
Cuts at: 3101 3461 3101
Size: 360 6215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 3201 4525 4706 4869 1840
Size: 1361 1324 181 163 3546
Fragments arranged by size:
3546 1361 1324 181 163
BtsI GCAGTG_nn'
Cuts at: 1297 3258 3818 1297
Size: 1961 560 4054
Fragments arranged by size:
4054 1961 560
ClaI AT'CG_AT
Cuts at: 794 794
Size: 6575
DraI TTT'AAA
Cuts at: 3574 3574
Size: 6575
DrdI GACnn_nn'nnGTC
Cuts at: 688 1825 2758 4281 5625 688
Size: 1137 933 1523 1344 1638
Fragments arranged by size:
1638 1523 1344 1137 933
EaeI y'GGCC_r
Cuts at: 566 2207 2796 3059 3242 4160 4334 4725 4752
Size: 1641 589 263 183 918 174 391 27
Cuts at: 4752 6097 566
Size: 1345 1044
Fragments arranged by size:
1641 1345 1044 918 589 391 263 183
174 27
EagI C'GGCC_G
Cuts at: 4160 4160
Size: 6575
EarI CTCTTCn'nnn_
Cuts at: 155 656 2939 3046 3334 4598 4808 155
Size: 501 2283 107 288 1264 210 1922
Fragments arranged by size:
2283 1922 1264 501 288 210 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1580 4783 5525 5671 6385 1580
Size: 3203 742 146 714 1770
Fragments arranged by size:
3203 1770 742 714 146
EcoICRI GAG'CTC
Cuts at: 1152 3587 1152
Size: 2435 4140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 604 862 886 934 955 1066 1150 1168
Size: 432 258 24 48 21 111 84 18
Cuts at: 1168 1243 1357 1420 1580 1633 1675 2026 2445
Size: 75 114 63 160 53 42 351 419
Cuts at: 2445 2512 2791 3289 3442 3457 4400 4832 5185
Size: 67 279 498 153 15 943 432 353
Cuts at: 5185 5847 172
Size: 662 900
Fragments arranged by size:
943 900 662 498 432 432 419 353
351 279 258 160 153 114 111 84
75 67 63 53 48 42 24 21
18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1087 1171 1423 2008 2227 3268 1087
Size: 84 252 585 219 1041 4394
Fragments arranged by size:
4394 1041 585 252 219 84
EcoO109I rG'GnC_Cy
Cuts at: 483 543 1041 1143 1986 2088 2095 2096 3441
Size: 60 498 102 843 102 7 1 1345
Cuts at: 3441 3714 483
Size: 273 3344
Fragments arranged by size:
3344 1345 843 498 273 102 102 60
7 1
EcoRI G'AATT_C
Cuts at: 3579 3579
Size: 6575
EcoRV GAT'ATC
Cuts at: 3129 3129
Size: 6575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 4594 2755
Size: 1839 4736
FspI TGC'GCA
Cuts at: 4356 4356
Size: 6575
HaeII r_GCGC'y
Cuts at: 1419 2652 2740 3027 4009 4257 5487 6258 1419
Size: 1233 88 287 982 248 1230 771 1736
Fragments arranged by size:
1736 1233 1230 982 771 287 248 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 943 975 1503 1535 3284 3316 4417 4447 4449
Size: 32 528 32 1749 32 1101 30 2
Cuts at: 4449 4479 943
Size: 30 3039
Fragments arranged by size:
3039 1749 1101 528 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1068 1395 2016 2367 2565 3614 1068
Size: 327 621 351 198 1049 4029
Fragments arranged by size:
4029 1049 621 351 327 198
HindIII A'AGCT_T
Cuts at: 2784 2784
Size: 6575
KasI G'GCGC_C
Cuts at: 1415 4005 4253 1415
Size: 2590 248 3737
Fragments arranged by size:
3737 2590 248
KpnI G_GTAC'C
Cuts at: 2382 3595 2382
Size: 1213 5362
MluI A'CGCG_T
Cuts at: 3203 6008 3203
Size: 2805 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 807 2270 3332 5334 5518 317
Size: 490 1463 1062 2002 184 1374
Fragments arranged by size:
2002 1463 1374 1062 490 184
MscI TGG'CCA
Cuts at: 568 2798 3061 4336 568
Size: 2230 263 1275 2807
Fragments arranged by size:
2807 2230 1275 263
MslI CAynn'nnrTG
Cuts at: 460 463 1210 1756 2071 2516 3337 3550 4564
Size: 3 747 546 315 445 821 213 1014
Cuts at: 4564 4691 460
Size: 127 2344
Fragments arranged by size:
2344 1014 821 747 546 445 315 213
127 3
MspA1I CmG'CkG
Cuts at: 582 1221 1626 1886 2241 2339 2469 3567 3686
Size: 639 405 260 355 98 130 1098 119
Cuts at: 3686 4000 4360 5144 5389 582
Size: 314 360 784 245 1768
Fragments arranged by size:
1768 1098 784 639 405 360 355 314
260 245 130 119 98
NaeI GCC'GGC
Cuts at: 331 2220 3031 3418 4756 331
Size: 1889 811 387 1338 2150
Fragments arranged by size:
2150 1889 1338 811 387
NarI GG'CG_CC
Cuts at: 1416 4006 4254 1416
Size: 2590 248 3737
Fragments arranged by size:
3737 2590 248
NcoI C'CATG_G
Cuts at: 3101 3101
Size: 6575
NgoMIV G'CCGG_C
Cuts at: 329 2218 3029 3416 4754 329
Size: 1889 811 387 1338 2150
Fragments arranged by size:
2150 1889 1338 811 387
NruI TCG'CGA
Cuts at: 2778 2778
Size: 6575
NsiI A_TGCA'T
Cuts at: 3217 3217
Size: 6575
NspI r_CATG'y
Cuts at: 204 2300 3480 3628 4128 5731 204
Size: 2096 1180 148 500 1603 1048
Fragments arranged by size:
2096 1603 1180 1048 500 148
PasI CC'CwG_GG
Cuts at: 1167 2092 1167
Size: 925 5650
PciI A'CATG_T
Cuts at: 3476 5727 3476
Size: 2251 4324
PflMI CCAn_nnn'nTGG
Cuts at: 2586 2586
Size: 6575
PfoI T'CCnGG_A
Cuts at: 635 1010 635
Size: 375 6200
PmeI GTTT'AAAC
Cuts at: 3574 3574
Size: 6575
PmlI CAC'GTG
Cuts at: 2082 2082
Size: 6575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3417 5021 5053 3385
Size: 32 1604 32 4907
Fragments arranged by size:
4907 1604 32 32
PpuMI rG'GwC_Cy
Cuts at: 543 1143 543
Size: 600 5975
PshAI GACnn'nnGTC
Cuts at: 1448 1448
Size: 6575
PspOMI G'GGCC_C
Cuts at: 2088 2095 2088
Size: 7 6568
PstI C_TGCA'G
Cuts at: 524 959 1088 1220 1631 2648 2726 3622 4307
Size: 435 129 132 411 1017 78 896 685
Cuts at: 4307 524
Size: 2792
Fragments arranged by size:
2792 1017 896 685 435 411 132 129
78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6575
PvuII CAG'CTG
Cuts at: 582 1221 1626 2241 2469 3567 4000 4360 582
Size: 639 405 615 228 1098 433 360 2797
Fragments arranged by size:
2797 1098 639 615 433 405 360 228
RsrII CG'GwC_CG
Cuts at: 4770 4770
Size: 6575
SacI G_AGCT'C
Cuts at: 1154 3589 1154
Size: 2435 4140
SalI G'TCGA_C
Cuts at: 2563 3612 2563
Size: 1049 5526
SapI GCTCTTCn'nnn_
Cuts at: 656 2939 3046 3334 4598 4808 656
Size: 2283 107 288 1264 210 2423
Fragments arranged by size:
2423 2283 1264 288 210 107
SbfI CC_TGCA'GG
Cuts at: 1088 3622 1088
Size: 2534 4041
ScaI AGT'ACT
Cuts at: 1285 1573 6092 1285
Size: 288 4519 1768
Fragments arranged by size:
4519 1768 288
SfcI C'TryA_G
Cuts at: 33 520 955 1084 1216 1627 2610 2644 2722
Size: 487 435 129 132 411 983 34 78
Cuts at: 2722 2994 3618 4303 5271 5462 33
Size: 272 624 685 968 191 1146
Fragments arranged by size:
1146 983 968 685 624 487 435 411
272 191 132 129 78 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 482 482
Size: 6575
SfoI GGC'GCC
Cuts at: 1417 4007 4255 1417
Size: 2590 248 3737
Fragments arranged by size:
3737 2590 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
SmaI CCC'GGG
Cuts at: 1811 2758 3597 1811
Size: 947 839 4789
Fragments arranged by size:
4789 947 839
SmlI C'TyrA_G
Cuts at: 787 1153 3730 4645 5359 5621 787
Size: 366 2577 915 714 262 1741
Fragments arranged by size:
2577 1741 915 714 366 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6575
SphI G_CATG'C
Cuts at: 3628 4128 3628
Size: 500 6075
StuI AGG'CCT
Cuts at: 1252 1384 1252
Size: 132 6443
StyI C'CwwG_G
Cuts at: 353 539 1424 1679 3101 3709 4063 353
Size: 186 885 255 1422 608 354 2865
Fragments arranged by size:
2865 1422 885 608 354 255 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2178 2704 3063 3540 4158 4291 4750 4959
Size: 126 526 359 477 618 133 459 209
Cuts at: 4959 2052
Size: 3668
Fragments arranged by size:
3668 618 526 477 459 359 209 133
126
TatI w'GTAC_w
Cuts at: 413 1187 1283 1313 1571 6090 413
Size: 774 96 30 258 4519 898
Fragments arranged by size:
4519 898 774 258 96 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 1141 1173 1948 1980 6092 6124 157
Size: 32 952 32 775 32 4112 32 608
Fragments arranged by size:
4112 952 775 608 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4372 4372
Size: 6575
XbaI T'CTAG_A
Cuts at: 3606 3606
Size: 6575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 4070 462
Size: 3608 2967
XhoI C'TCGA_G
Cuts at: 787 787
Size: 6575
XmaI C'CCGG_G
Cuts at: 1809 2756 3595 1809
Size: 947 839 4789
Fragments arranged by size:
4789 947 839
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AscI AvrII BaeI BmtI BsiWI
BstZ17I Bsu36I CspCI DraIII FseI FspAI HpaI MfeI
NdeI NheI NotI PacI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI