Restriction map for
pF1KF0137
(Circular) (Six-base) MAPSORT of: pF1KF0137.seq Check: 1084 from: 1 to: 7637
pF1KF0137 7637 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3702 3702
Size: 7637
AccI GT'mk_AC
Cuts at: 2157 3447 4675 2157
Size: 1290 1228 5119
Fragments arranged by size:
5119 1290 1228
Acc65I G'GTAC_C
Cuts at: 4653 4653
Size: 7637
AclI AA'CG_TT
Cuts at: 7477 7477
Size: 7637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 937 1062 1969 3193 3594 4097 4106 4253
Size: 188 125 907 1224 401 503 9 147
Cuts at: 4253 4561 5462 5894 6641 6909 749
Size: 308 901 432 747 268 1477
Fragments arranged by size:
1477 1224 907 901 747 503 432 401
308 268 188 147 125 9
AfeI AGC'GCT
Cuts at: 1400 1400
Size: 7637
AflIII A'CryG_T
Cuts at: 4989 6093 7070 4989
Size: 1104 977 5556
Fragments arranged by size:
5556 1104 977
AgeI A'CCGG_T
Cuts at: 6025 6025
Size: 7637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 410 442 410
Size: 32 7605
AlwNI CAG_nnn'CTG
Cuts at: 2422 2974 3654 3708 4010 4942 6509 2422
Size: 552 680 54 302 932 1567 3550
Fragments arranged by size:
3550 1567 932 680 552 302 54
ApaI G_GGCC'C
Cuts at: 235 235
Size: 7637
ApaLI G'TGCA_C
Cuts at: 1939 2227 6407 1939
Size: 288 4180 3169
Fragments arranged by size:
4180 3169 288
ApoI r'AATT_y
Cuts at: 1365 3967 3999 4447 4641 6990 7246 1365
Size: 2602 32 448 194 2349 256 1756
Fragments arranged by size:
2602 2349 1756 448 256 194 32
AseI AT'TA_AT
Cuts at: 20 2567 20
Size: 2547 5090
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7637
AvaI C'yCGr_G
Cuts at: 178 234 464 4657 178
Size: 56 230 4193 3158
Fragments arranged by size:
4193 3158 230 56
BamHI G'GATC_C
Cuts at: 2187 4662 2187
Size: 2475 5162
BanI G'GyrC_C
Cuts at: 1409 2964 3559 4653 5067 5315 5350 1409
Size: 1555 595 1094 414 248 35 3696
Fragments arranged by size:
3696 1555 1094 595 414 248 35
BanII G_rGCy'C
Cuts at: 235 2000 2053 2129 3420 4184 4574 4651 5681
Size: 1765 53 76 1291 764 390 77 1030
Cuts at: 5681 235
Size: 2191
Fragments arranged by size:
2191 1765 1291 1030 764 390 77 76
53
BbeI G_GCGC'C
Cuts at: 5071 5319 5071
Size: 248 7389
BbsI GAAGACnn'nnnn_
Cuts at: 207 1525 1957 4614 207
Size: 1318 432 2657 3230
Fragments arranged by size:
3230 2657 1318 432
BbvCI CC'TCA_GC
Cuts at: 3187 3187
Size: 7637
BciVI GTATCCnnnnn_n'
Cuts at: 5535 6296 7637 5535
Size: 761 1341 5535
Fragments arranged by size:
5535 1341 761
BclI T'GATC_A
Cuts at: 5157 5157
Size: 7637
BglI GCCn_nnn'nGGC
Cuts at: 191 4116 191
Size: 3925 3712
BglII A'GATC_T
Cuts at: 2898 5152 7189 2898
Size: 2254 2037 3346
Fragments arranged by size:
3346 2254 2037
BlpI GC'TnA_GC
Cuts at: 1431 3745 4749 1431
Size: 2314 1004 4319
Fragments arranged by size:
4319 2314 1004
Bme1580I G_kGCm'C
Cuts at: 179 235 1943 2231 3384 3562 5262 5355 6411
Size: 56 1708 288 1153 178 1700 93 1056
Cuts at: 6411 179
Size: 1405
Fragments arranged by size:
1708 1700 1405 1153 1056 288 178 93
56
BmrI ACTGGGnnnn_n'
Cuts at: 3160 3417 4849 4930 5265 7409 3160
Size: 257 1432 81 335 2144 3388
Fragments arranged by size:
3388 2144 1432 335 257 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 6004 2657
Size: 3347 4290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 888 1964 2480 2513 2718 2899 3499 3520
Size: 94 1076 516 33 205 181 600 21
Cuts at: 3520 3969 4510 794
Size: 449 541 3921
Fragments arranged by size:
3921 1076 600 541 516 449 205 181
94 33 21
Bpu10I CC'TnA_GC
Cuts at: 3187 3626 3187
Size: 439 7198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2373 3085 4155 4813 5692 6184 6482 2255
Size: 118 712 1070 658 879 492 298 3410
Fragments arranged by size:
3410 1070 879 712 658 492 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 312 464 2183 3983 4237 7341 312
Size: 152 1719 1800 254 3104 608
Fragments arranged by size:
3104 1800 1719 608 254 152
BsaAI yAC'GTr
Cuts at: 1938 4990 1938
Size: 3052 4585
BsaBI GATnn'nnATC
Cuts at: 6 5151 7188 6
Size: 5145 2037 455
Fragments arranged by size:
5145 2037 455
BsaHI Gr'CG_yC
Cuts at: 3957 5068 5316 6018 7514 3957
Size: 1111 248 702 1496 4080
Fragments arranged by size:
4080 1496 1111 702 248
BsaWI w'CCGG_w
Cuts at: 2003 3803 4323 4826 5347 6025 6299 6446 6857
Size: 1800 520 503 521 678 274 147 411
Cuts at: 6857 2003
Size: 2783
Fragments arranged by size:
2783 1800 678 521 520 503 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 272 302 410 440 1462 1492 3472 3502 272
Size: 30 108 30 1022 30 1980 30 4407
Fragments arranged by size:
4407 1980 1022 108 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 452 941 1586 1628 2258 2470 2704 3522 3525
Size: 489 645 42 630 212 234 818 3
Cuts at: 3525 452
Size: 4564
Fragments arranged by size:
4564 818 645 630 489 234 212 42
3
BseYI C'CCAG_C
Cuts at: 982 3191 5062 5820 6397 6893 982
Size: 2209 1871 758 577 496 1726
Fragments arranged by size:
2209 1871 1726 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 3799 1239
Size: 1269 1291 5077
Fragments arranged by size:
5077 1291 1269
BsiEI CG_ry'CG
Cuts at: 104 5179 5225 6433 104
Size: 5075 46 1208 1308
Fragments arranged by size:
5075 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1943 2231 4184 4574 4651 5429 5619 6411 1943
Size: 288 1953 390 77 778 190 792 3169
Fragments arranged by size:
3169 1953 792 778 390 288 190 77
BsmI GAATG_Cn'
Cuts at: 798 4166 798
Size: 3368 4269
BsmBI CGTCTCn'nnnn_
Cuts at: 1053 1053
Size: 7637
Bsp1286I G_dGCh'C
Cuts at: 179 235 1943 2000 2053 2129 2231 3384 3420
Size: 56 1708 57 53 76 102 1153 36
Cuts at: 3420 3562 4184 4574 4651 5262 5355 5429 5619
Size: 142 622 390 77 611 93 74 190
Cuts at: 5619 5681 6411 179
Size: 62 730 1405
Fragments arranged by size:
1708 1405 1153 730 622 611 390 190
142 102 93 77 76 74 62 57
56 53 36
BspEI T'CCGG_A
Cuts at: 3803 6857 3803
Size: 3054 4583
BspHI T'CATG_A
Cuts at: 594 1869 2058 6813 594
Size: 1275 189 4755 1418
Fragments arranged by size:
4755 1418 1275 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3702 4687 4901 5203 5584 3702
Size: 985 214 302 381 5755
Fragments arranged by size:
5755 985 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5929 5983 7467 7632 50
Size: 5879 54 1484 165 55
Fragments arranged by size:
5879 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1042 1776 5549 1042
Size: 734 3773 3130
Fragments arranged by size:
3773 3130 734
BsrFI r'CCGG_y
Cuts at: 5635 5816 6025 5635
Size: 181 209 7247
Fragments arranged by size:
7247 209 181
BsrGI T'GTAC_A
Cuts at: 1719 1719
Size: 7637
BssSI C'ACGA_G
Cuts at: 5908 6266 5908
Size: 358 7279
BstAPI GCAn_nnn'nTGC
Cuts at: 5014 5014
Size: 7637
BstBI TT'CG_AA
Cuts at: 861 5998 861
Size: 5137 2500
BstXI CCAn_nnnn'nTGG
Cuts at: 3282 3498 4531 3282
Size: 216 1033 6388
Fragments arranged by size:
6388 1033 216
BstYI r'GATC_y
Cuts at: 1 1041 2187 2680 2898 4662 5129 5152 5487
Size: 1040 1146 493 218 1764 467 23 335
Cuts at: 5487 5733 6745 6831 6843 7189 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1764 1146 1040 1012 493 467 449 346
335 246 218 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2318 2538 2318
Size: 220 7417
BtgI C'CryG_G
Cuts at: 107 121 107
Size: 14 7623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 182 5587 5768 5931 182
Size: 5405 181 163 1888
Fragments arranged by size:
5405 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 3599 3746 4064 4611 4880 3599
Size: 147 318 547 269 6356
Fragments arranged by size:
6356 547 318 269 147
ClaI AT'CG_AT
Cuts at: 2377 2377
Size: 7637
DraI TTT'AAA
Cuts at: 4636 4636
Size: 7637
DraIII CAC_nnn'GTG
Cuts at: 3078 3078
Size: 7637
DrdI GACnn_nn'nnGTC
Cuts at: 2153 5343 6201 2153
Size: 3190 858 3589
Fragments arranged by size:
3589 3190 858
EaeI y'GGCC_r
Cuts at: 1292 2024 2856 3216 3284 3398 5222 5396 5787
Size: 732 832 360 68 114 1824 174 391
Cuts at: 5787 5814 7159 1292
Size: 27 1345 1770
Fragments arranged by size:
1824 1770 1345 832 732 391 360 174
114 68 27
EagI C'GGCC_G
Cuts at: 5222 5222
Size: 7637
EarI CTCTTCn'nnn_
Cuts at: 913 918 1081 1275 2117 2626 3595 5660 5870
Size: 5 163 194 842 509 969 2065 210
Cuts at: 5870 913
Size: 2680
Fragments arranged by size:
2680 2065 969 842 509 210 194 163
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5845 6155 6301 7447 5845
Size: 310 146 1146 6035
Fragments arranged by size:
6035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4182 4572 4649 4182
Size: 390 77 7170
Fragments arranged by size:
7170 390 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 794 888 937 1062 1964 1969 2480 2513
Size: 45 94 49 125 902 5 511 33
Cuts at: 2513 2718 2899 3193 3499 3520 3594 3969 4097
Size: 205 181 294 306 21 74 375 128
Cuts at: 4097 4106 4253 4510 4561 5462 5894 6641 6909
Size: 9 147 257 51 901 432 747 268
Cuts at: 6909 749
Size: 1477
Fragments arranged by size:
1477 902 901 747 511 432 375 306
294 268 257 205 181 147 128 125
94 74 51 49 45 33 21 9
5
EcoNI CCTnn'n_nnAGG
Cuts at: 2270 2270
Size: 7637
EcoO109I rG'GnC_Cy
Cuts at: 183 2419 3162 3568 4520 4776 183
Size: 2236 743 406 952 256 3044
Fragments arranged by size:
3044 2236 952 743 406 256
EcoRI G'AATT_C
Cuts at: 3967 4641 3967
Size: 674 6963
EcoRV GAT'ATC
Cuts at: 443 443
Size: 7637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 976 2367 2710 3409 3624 4200 4591 5656 976
Size: 1391 343 699 215 576 391 1065 2957
Fragments arranged by size:
2957 1391 1065 699 576 391 343 215
FspI TGC'GCA
Cuts at: 5418 5418
Size: 7637
HaeII r_GCGC'y
Cuts at: 960 1402 3787 5071 5319 6341 7320 960
Size: 442 2385 1284 248 1022 979 1277
Fragments arranged by size:
2385 1284 1277 1022 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1270 1302 1462 1494 2040 2072 2665
Size: 32 511 32 160 32 546 32 593
Cuts at: 2665 2697 3289 3321 3340 3372 3472 3504 3889
Size: 32 592 32 19 32 100 32 385
Cuts at: 3889 3921 5479 5509 5511 5541 727
Size: 32 1558 30 2 30 2823
Fragments arranged by size:
2823 1558 593 592 546 511 385 160
100 32 32 32 32 32 32 32
32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 968 2158 4282 4409 4676 968
Size: 1190 2124 127 267 3929
Fragments arranged by size:
3929 2124 1190 267 127
HindIII A'AGCT_T
Cuts at: 1648 1843 3005 1648
Size: 195 1162 6280
Fragments arranged by size:
6280 1162 195
HpaI GTT'AAC
Cuts at: 4409 4409
Size: 7637
KasI G'GCGC_C
Cuts at: 5067 5315 5067
Size: 248 7389
KpnI G_GTAC'C
Cuts at: 4657 4657
Size: 7637
MluI A'CGCG_T
Cuts at: 7070 7070
Size: 7637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 1505 1717 1854 1943 4287 6308 6492 389
Size: 1116 212 137 89 2344 2021 184 1534
Fragments arranged by size:
2344 2021 1534 1116 212 184 137 89
MscI TGG'CCA
Cuts at: 1294 2858 3218 3286 3400 5398 1294
Size: 1564 360 68 114 1998 3533
Fragments arranged by size:
3533 1998 1564 360 114 68
MslI CAynn'nnrTG
Cuts at: 634 692 1874 1936 2060 2245 3832 5626 5753
Size: 58 1182 62 124 185 1587 1794 127
Cuts at: 5753 634
Size: 2518
Fragments arranged by size:
2518 1794 1587 1182 185 127 124 62
58
MspA1I CmG'CkG
Cuts at: 123 161 1002 1158 1223 1607 2500 2974 3021
Size: 38 841 156 65 384 893 474 47
Cuts at: 3021 3191 3435 4748 5062 5422 6435 6680 123
Size: 170 244 1313 314 360 1013 245 1080
Fragments arranged by size:
1313 1080 1013 893 841 474 384 360
314 245 244 170 156 65 47 38
NaeI GCC'GGC
Cuts at: 5818 5818
Size: 7637
NarI GG'CG_CC
Cuts at: 5068 5316 5068
Size: 248 7389
NcoI C'CATG_G
Cuts at: 107 107
Size: 7637
NgoMIV G'CCGG_C
Cuts at: 5816 5816
Size: 7637
NspI r_CATG'y
Cuts at: 424 4201 4690 5190 6097 424
Size: 3777 489 500 907 1964
Fragments arranged by size:
3777 1964 907 500 489
PciI A'CATG_T
Cuts at: 6093 6093
Size: 7637
PflMI CCAn_nnn'nTGG
Cuts at: 713 1862 2974 3407 3756 4010 713
Size: 1149 1112 433 349 254 4340
Fragments arranged by size:
4340 1149 1112 433 349 254
PfoI T'CCnGG_A
Cuts at: 2117 2117
Size: 7637
PmeI GTTT'AAAC
Cuts at: 4636 4636
Size: 7637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 410 442 6776 6808 410
Size: 32 6334 32 1239
Fragments arranged by size:
6334 1239 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 3162 4520 2419
Size: 743 1358 5536
Fragments arranged by size:
5536 1358 743
PshAI GACnn'nnGTC
Cuts at: 211 211
Size: 7637
PspOMI G'GGCC_C
Cuts at: 231 231
Size: 7637
PspXI vC'TCGA_Gb
Cuts at: 464 464
Size: 7637
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3652 3684 3652
Size: 32 7605
PstI C_TGCA'G
Cuts at: 166 2925 3324 3434 3594 3659 4354 4684 5369
Size: 2759 399 110 160 65 695 330 685
Cuts at: 5369 166
Size: 2434
Fragments arranged by size:
2759 2434 695 685 399 330 160 110
65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7637
PvuII CAG'CTG
Cuts at: 1002 1158 1223 1607 2974 3021 3191 3435 5062
Size: 156 65 384 1367 47 170 244 1627
Cuts at: 5062 5422 1002
Size: 360 3217
Fragments arranged by size:
3217 1627 1367 384 360 244 170 156
65 47
RsrII CG'GwC_CG
Cuts at: 5832 5832
Size: 7637
SacI G_AGCT'C
Cuts at: 4184 4574 4651 4184
Size: 390 77 7170
Fragments arranged by size:
7170 390 77
SacII CC_GC'GG
Cuts at: 124 124
Size: 7637
SalI G'TCGA_C
Cuts at: 2156 4674 2156
Size: 2518 5119
SapI GCTCTTCn'nnn_
Cuts at: 2117 3595 5660 5870 2117
Size: 1478 2065 210 3884
Fragments arranged by size:
3884 2065 1478 210
SbfI CC_TGCA'GG
Cuts at: 4684 4684
Size: 7637
ScaI AGT'ACT
Cuts at: 4170 7154 4170
Size: 2984 4653
SfcI C'TryA_G
Cuts at: 33 162 797 2921 3227 3320 3430 3448 3590
Size: 129 635 2124 306 93 110 18 142
Cuts at: 3590 3655 4350 4680 5365 6358 6549 33
Size: 65 695 330 685 993 191 1121
Fragments arranged by size:
2124 1121 993 695 685 635 330 306
191 142 129 110 93 65 18
SfoI GGC'GCC
Cuts at: 5069 5317 5069
Size: 248 7389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7637
SmaI CCC'GGG
Cuts at: 180 236 4659 180
Size: 56 4423 3158
Fragments arranged by size:
4423 3158 56
SmlI C'TyrA_G
Cuts at: 464 2270 2352 3100 4134 4792 5707 6199 6461
Size: 1806 82 748 1034 658 915 492 262
Cuts at: 6461 464
Size: 1640
Fragments arranged by size:
1806 1640 1034 915 748 658 492 262
82
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7637
SphI G_CATG'C
Cuts at: 4201 4690 5190 4201
Size: 489 500 6648
Fragments arranged by size:
6648 500 489
SrfI GCCC'GGGC
Cuts at: 236 236
Size: 7637
SspI AAT'ATT
Cuts at: 1793 1793
Size: 7637
StuI AGG'CCT
Cuts at: 3940 4119 4468 3940
Size: 179 349 7109
Fragments arranged by size:
7109 349 179
StyI C'CwwG_G
Cuts at: 107 1211 1239 1388 1418 3140 4247 4771 5125
Size: 1104 28 149 30 1722 1107 524 354
Cuts at: 5125 107
Size: 2619
Fragments arranged by size:
2619 1722 1107 1104 524 354 149 30
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1427 3399 5220 5353 5812 6021 1427
Size: 1972 1821 133 459 209 3043
Fragments arranged by size:
3043 1972 1821 459 209 133
TatI w'GTAC_w
Cuts at: 341 1719 1822 2662 3845 4168 7152 341
Size: 1378 103 840 1183 323 2984 826
Fragments arranged by size:
2984 1378 1183 840 826 323 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 272 304 1638 1670 3049 3081 7154 7186 272
Size: 32 1334 32 1379 32 4073 32 723
Fragments arranged by size:
4073 1379 1334 723 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3166 5434 3166
Size: 2268 5369
XbaI T'CTAG_A
Cuts at: 877 2506 4668 877
Size: 1629 2162 3846
Fragments arranged by size:
3846 2162 1629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3408 5132 3408
Size: 1724 5913
XhoI C'TCGA_G
Cuts at: 464 464
Size: 7637
XmaI C'CCGG_G
Cuts at: 178 234 4657 178
Size: 56 4423 3158
Fragments arranged by size:
4423 3158 56
XmnI GAAnn'nnTTC
Cuts at: 668 1793 2777 3644 668
Size: 1125 984 867 4661
Fragments arranged by size:
4661 1125 984 867
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BssHII BstEII BstZ17I
CspCI FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI SanDI SexAI SfiI
SgrAI SnaBI SwaI ZraI