Restriction map for
pF1KF0141
(Circular) (Six-base) MAPSORT of: pF1KF0141.seq Check: 8946 from: 1 to: 7070
pF1KF0141 7070 bp
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3257 3917 3257
Size: 660 6410
AccI GT'mk_AC
Cuts at: 4108 4108
Size: 7070
Acc65I G'GTAC_C
Cuts at: 960 4086 960
Size: 3126 3944
AclI AA'CG_TT
Cuts at: 4064 6910 4064
Size: 2846 4224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1692 3151 3691 3880 4895 5327 5680 6342
Size: 1156 1459 540 189 1015 432 353 662
Cuts at: 6342 536
Size: 1264
Fragments arranged by size:
1459 1264 1156 1015 662 540 432 353
189
AfeI AGC'GCT
Cuts at: 2149 2988 3793 2149
Size: 839 805 5426
Fragments arranged by size:
5426 839 805
AflII C'TTAA_G
Cuts at: 1485 1485
Size: 7070
AflIII A'CryG_T
Cuts at: 3374 4422 6222 6503 3374
Size: 1048 1800 281 3941
Fragments arranged by size:
3941 1800 1048 281
AhdI GACnn_n'nnGTC
Cuts at: 3375 3375
Size: 7070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2231 2231
Size: 7070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2518 2550 2518
Size: 32 7038
AlwNI CAG_nnn'CTG
Cuts at: 1120 1213 1273 1947 2277 2328 2595 2745 3504
Size: 93 60 674 330 51 267 150 759
Cuts at: 3504 3576 4039 4375 5813 1120
Size: 72 463 336 1438 2377
Fragments arranged by size:
2377 1438 759 674 463 336 330 267
150 93 72 60 51
ApaI G_GGCC'C
Cuts at: 340 569 3205 340
Size: 229 2636 4205
Fragments arranged by size:
4205 2636 229
ApaLI G'TGCA_C
Cuts at: 5908 5908
Size: 7070
ApoI r'AATT_y
Cuts at: 4074 6423 6679 4074
Size: 2349 256 4465
Fragments arranged by size:
4465 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7070
AvaI C'yCGr_G
Cuts at: 2342 2717 3207 4090 2342
Size: 375 490 883 5322
Fragments arranged by size:
5322 883 490 375
BamHI G'GATC_C
Cuts at: 4095 4095
Size: 7070
BanI G'GyrC_C
Cuts at: 382 826 960 1689 1812 4086 4500 4748 4783
Size: 444 134 729 123 2274 414 248 35
Cuts at: 4783 382
Size: 2669
Fragments arranged by size:
2669 2274 729 444 414 248 134 123
35
BanII G_rGCy'C
Cuts at: 340 431 561 569 682 844 1156 1512 1663
Size: 91 130 8 113 162 312 356 151
Cuts at: 1663 1726 1744 2271 2688 3205 3316 3931 4084
Size: 63 18 527 417 517 111 615 153
Cuts at: 4084 5114 340
Size: 1030 2296
Fragments arranged by size:
2296 1030 615 527 517 417 356 312
162 153 151 130 113 111 91 63
18 8
BbeI G_GCGC'C
Cuts at: 830 4504 4752 830
Size: 3674 248 3148
Fragments arranged by size:
3674 3148 248
BbsI GAAGACnn'nnnn_
Cuts at: 2827 3679 2827
Size: 852 6218
BbvCI CC'TCA_GC
Cuts at: 3149 4040 3149
Size: 891 6179
BciVI GTATCCnnnnn_n'
Cuts at: 4968 6024 7070 4968
Size: 1056 1046 4968
Fragments arranged by size:
4968 1056 1046
BclI T'GATC_A
Cuts at: 4590 4590
Size: 7070
BglI GCCn_nnn'nGGC
Cuts at: 450 1454 1603 2347 3209 3258 450
Size: 1004 149 744 862 49 4262
Fragments arranged by size:
4262 1004 862 744 149 49
BglII A'GATC_T
Cuts at: 4585 6622 4585
Size: 2037 5033
BlpI GC'TnA_GC
Cuts at: 1045 1720 2612 2947 3125 3727 4182 1045
Size: 675 892 335 178 602 455 3933
Fragments arranged by size:
3933 892 675 602 455 335 178
Bme1580I G_kGCm'C
Cuts at: 340 385 569 953 1641 2285 2367 2400 3205
Size: 45 184 384 688 644 82 33 805
Cuts at: 3205 3564 4695 4788 5912 340
Size: 359 1131 93 1124 1498
Fragments arranged by size:
1498 1131 1124 805 688 644 384 359
184 93 82 45 33
BmrI ACTGGGnnnn_n'
Cuts at: 917 1684 1760 4282 4363 4698 6842 917
Size: 767 76 2522 81 335 2144 1145
Fragments arranged by size:
2522 2144 1145 767 335 81 76
BmtI G_CTAG'C
Cuts at: 2251 2251
Size: 7070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 876 1638 5437 876
Size: 762 3799 2509
Fragments arranged by size:
3799 2509 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 335 755 828 848 960 1250 1285 1319
Size: 31 420 73 20 112 290 35 34
Cuts at: 1319 1779 1897 1969 2218 2521 3328 3526 304
Size: 460 118 72 249 303 807 198 3848
Fragments arranged by size:
3848 807 460 420 303 290 249 198
118 112 73 72 35 34 31 20
Bpu10I CC'TnA_GC
Cuts at: 2270 2687 3149 4040 2270
Size: 417 462 891 5300
Fragments arranged by size:
5300 891 462 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 2281 3799 4246 5125 5839 6137 2152
Size: 129 1518 447 879 714 298 3085
Fragments arranged by size:
3085 1518 879 714 447 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 1945 3049 3322 6774 1945
Size: 1104 273 3452 2241
Fragments arranged by size:
3452 2241 1104 273
BsaAI yAC'GTr
Cuts at: 2286 3720 4423 2286
Size: 1434 703 4933
Fragments arranged by size:
4933 1434 703
BsaBI GATnn'nnATC
Cuts at: 6 4584 6621 6
Size: 4578 2037 455
Fragments arranged by size:
4578 2037 455
BsaHI Gr'CG_yC
Cuts at: 827 4501 4749 5451 6947 827
Size: 3674 248 702 1496 950
Fragments arranged by size:
3674 1496 950 702 248
BsaWI w'CCGG_w
Cuts at: 2600 3650 4259 4780 5458 5869 6016 6290 2600
Size: 1050 609 521 678 411 147 274 3380
Fragments arranged by size:
3380 1050 678 609 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 742 2119 2188 2581 2584 2904 2907 2922 2925
Size: 1377 69 393 3 320 3 15 3
Cuts at: 2925 2928 3289 3427 3523 3526 742
Size: 3 361 138 96 3 4286
Fragments arranged by size:
4286 1377 393 361 320 138 96 69
15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1075 1161 1206 1315 1589 1632 1725 2005 2017
Size: 86 45 109 274 43 93 280 12
Cuts at: 2017 2198 2277 2671 2942 3195 3936 4495 5253
Size: 181 79 394 271 253 741 559 758
Cuts at: 5253 5918 6326 1075
Size: 665 408 1819
Fragments arranged by size:
1819 758 741 665 559 408 394 280
274 271 253 181 109 93 86 79
45 43 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 399 2199 2716 3700 3938 184
Size: 215 1800 517 984 238 3316
Fragments arranged by size:
3316 1800 984 517 238 215
BsiEI CG_ry'CG
Cuts at: 104 4612 4658 5888 104
Size: 4508 46 1230 1286
Fragments arranged by size:
4508 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 359 682 1026 1221 1512 2197 2210 3287 3316
Size: 323 344 195 291 685 13 1077 29
Cuts at: 3316 3728 4084 4862 5052 5912 359
Size: 412 356 778 190 860 1517
Fragments arranged by size:
1517 1077 860 778 685 412 356 344
323 291 195 190 29 13
BsmI GAATG_Cn'
Cuts at: 1238 2733 1238
Size: 1495 5575
BsmBI CGTCTCn'nnnn_
Cuts at: 3844 3844
Size: 7070
Bsp1286I G_dGCh'C
Cuts at: 340 359 385 431 561 569 682 844 953
Size: 19 26 46 130 8 113 162 109
Cuts at: 953 1026 1156 1221 1512 1641 1663 1726 1744
Size: 73 130 65 291 129 22 63 18
Cuts at: 1744 2197 2210 2271 2285 2367 2400 2688 3205
Size: 453 13 61 14 82 33 288 517
Cuts at: 3205 3287 3316 3564 3728 3931 4084 4695 4788
Size: 82 29 248 164 203 153 611 93
Cuts at: 4788 4862 5052 5114 5912 340
Size: 74 190 62 798 1498
Fragments arranged by size:
1498 798 611 517 453 291 288 248
203 190 164 162 153 130 130 129
113 109 93 82 82 74 73 65
63 62 61 46 33 29 26 22
19 18 14 13 8
BspEI T'CCGG_A
Cuts at: 2600 3650 2600
Size: 1050 6020
BspHI T'CATG_A
Cuts at: 4058 5502 4058
Size: 1444 5626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 704 809 972 3257 3522 3650 3718 3917
Size: 321 105 163 2285 265 128 68 199
Cuts at: 3917 4120 4334 4636 5017 383
Size: 203 214 302 381 2436
Fragments arranged by size:
2436 2285 381 321 302 265 214 203
199 163 128 105 68
BsrBI CCG'CTC
Cuts at: 50 1347 1830 1905 2265 2361 2394 2550 2852
Size: 1297 483 75 360 96 33 156 302
Cuts at: 2852 3477 3516 5362 5416 6900 7065 50
Size: 625 39 1846 54 1484 165 55
Fragments arranged by size:
1846 1484 1297 625 483 360 302 165
156 96 75 55 54 39 33
BsrDI GCAATG_nn'
Cuts at: 414 4982 414
Size: 4568 2502
BsrFI r'CCGG_y
Cuts at: 1448 1886 2584 3455 3809 3968 5068 5249 1448
Size: 438 698 871 354 159 1100 181 3269
Fragments arranged by size:
3269 1100 871 698 438 354 181 159
BssSI C'ACGA_G
Cuts at: 2069 5341 6049 2069
Size: 3272 708 3090
Fragments arranged by size:
3272 3090 708
BstAPI GCAn_nnn'nTGC
Cuts at: 413 473 2328 3723 3740 4447 413
Size: 60 1855 1395 17 707 3036
Fragments arranged by size:
3036 1855 1395 707 60 17
BstBI TT'CG_AA
Cuts at: 5431 5431
Size: 7070
BstXI CCAn_nnnn'nTGG
Cuts at: 837 837
Size: 7070
BstYI r'GATC_y
Cuts at: 1 1542 2678 4095 4562 4585 4920 5166 5472
Size: 1541 1136 1417 467 23 335 246 306
Cuts at: 5472 5484 5570 6622 1
Size: 12 86 1052 449
Fragments arranged by size:
1541 1417 1136 1052 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2000 2000
Size: 7070
BtgI C'CryG_G
Cuts at: 107 158 1599 3696 3744 107
Size: 51 1441 2097 48 3433
Fragments arranged by size:
3433 2097 1441 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2613 5020 5201 5364 2613
Size: 2407 181 163 4319
Fragments arranged by size:
4319 2407 181 163
BtsI GCAGTG_nn'
Cuts at: 252 357 903 1347 2365 2398 2429 3040 3921
Size: 105 546 444 1018 33 31 611 881
Cuts at: 3921 4313 252
Size: 392 3009
Fragments arranged by size:
3009 1018 881 611 546 444 392 105
33 31
DraI TTT'AAA
Cuts at: 4069 4069
Size: 7070
DraIII CAC_nnn'GTG
Cuts at: 234 925 1678 3723 234
Size: 691 753 2045 3581
Fragments arranged by size:
3581 2045 753 691
DrdI GACnn_nn'nnGTC
Cuts at: 4776 6120 4776
Size: 1344 5726
EaeI y'GGCC_r
Cuts at: 1184 2582 2586 2651 4655 4829 5220 5247 6592
Size: 1398 4 65 2004 174 391 27 1345
Cuts at: 6592 1184
Size: 1662
Fragments arranged by size:
2004 1662 1398 1345 391 174 65 27
4
EagI C'GGCC_G
Cuts at: 4655 4655
Size: 7070
EarI CTCTTCn'nnn_
Cuts at: 888 2255 2931 3098 5093 5303 888
Size: 1367 676 167 1995 210 2655
Fragments arranged by size:
2655 1995 1367 676 210 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1144 2109 3534 5278 6020 6166 6880 1144
Size: 965 1425 1744 742 146 714 1334
Fragments arranged by size:
1744 1425 1334 965 742 714 146
EcoICRI GAG'CTC
Cuts at: 680 1510 3314 4082 680
Size: 830 1804 768 3668
Fragments arranged by size:
3668 1804 830 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 335 536 755 828 848 960 1250 1285
Size: 31 201 219 73 20 112 290 35
Cuts at: 1285 1319 1692 1779 1897 1969 2218 2521 3151
Size: 34 373 87 118 72 249 303 630
Cuts at: 3151 3328 3526 3691 3880 4895 5327 5680 6342
Size: 177 198 165 189 1015 432 353 662
Cuts at: 6342 304
Size: 1032
Fragments arranged by size:
1032 1015 662 630 432 373 353 303
290 249 219 201 198 189 177 165
118 112 87 73 72 35 34 31
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2839 3508 2839
Size: 669 6401
EcoO109I rG'GnC_Cy
Cuts at: 136 172 336 566 643 693 940 1455 1996
Size: 36 164 230 77 50 247 515 541
Cuts at: 1996 2063 2084 2496 3202 3501 4209 136
Size: 67 21 412 706 299 708 2997
Fragments arranged by size:
2997 708 706 541 515 412 299 247
230 164 77 67 50 36 21
EcoRI G'AATT_C
Cuts at: 4074 4074
Size: 7070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1722 3678 5089 1722
Size: 1956 1411 3703
Fragments arranged by size:
3703 1956 1411
FseI GG_CCGG'CC
Cuts at: 2588 2588
Size: 7070
FspI TGC'GCA
Cuts at: 3913 4851 3913
Size: 938 6132
HaeII r_GCGC'y
Cuts at: 798 830 1590 1899 1947 2151 2649 2990 3066
Size: 32 760 309 48 204 498 341 76
Cuts at: 3066 3795 4504 4752 5982 6753 798
Size: 729 709 248 1230 771 1115
Fragments arranged by size:
1230 1115 771 760 729 709 498 341
309 248 204 76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2670 2702 3361 3393 4912 4942 4944 4974 2670
Size: 32 659 32 1519 30 2 30 4766
Fragments arranged by size:
4766 1519 659 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1228 4109 1228
Size: 2881 4189
KasI G'GCGC_C
Cuts at: 826 4500 4748 826
Size: 3674 248 3148
Fragments arranged by size:
3674 3148 248
KpnI G_GTAC'C
Cuts at: 964 4090 964
Size: 3126 3944
MluI A'CGCG_T
Cuts at: 6503 6503
Size: 7070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2525 5829 6013 2525
Size: 3304 184 3582
Fragments arranged by size:
3582 3304 184
MscI TGG'CCA
Cuts at: 1186 2653 4831 1186
Size: 1467 2178 3425
Fragments arranged by size:
3425 2178 1467
MslI CAynn'nnrTG
Cuts at: 835 2213 3290 5059 5186 835
Size: 1378 1077 1769 127 2719
Fragments arranged by size:
2719 1769 1378 1077 127
MspA1I CmG'CkG
Cuts at: 282 957 1173 1521 1799 2328 2433 2742 2900
Size: 675 216 348 278 529 105 309 158
Cuts at: 2900 3111 3148 3199 3576 4181 4495 4855 5639
Size: 211 37 51 377 605 314 360 784
Cuts at: 5639 5884 282
Size: 245 1468
Fragments arranged by size:
1468 784 675 605 529 377 360 348
314 309 278 245 216 211 158 105
51 37
NaeI GCC'GGC
Cuts at: 1450 2586 5251 1450
Size: 1136 2665 3269
Fragments arranged by size:
3269 2665 1136
NarI GG'CG_CC
Cuts at: 827 4501 4749 827
Size: 3674 248 3148
Fragments arranged by size:
3674 3148 248
NcoI C'CATG_G
Cuts at: 107 158 1599 3696 107
Size: 51 1441 2097 3481
Fragments arranged by size:
3481 2097 1441 51
NgoMIV G'CCGG_C
Cuts at: 1448 2584 5249 1448
Size: 1136 2665 3269
Fragments arranged by size:
3269 2665 1136
NheI G'CTAG_C
Cuts at: 2247 2247
Size: 7070
NspI r_CATG'y
Cuts at: 471 3378 4123 4623 6226 471
Size: 2907 745 500 1603 1315
Fragments arranged by size:
2907 1603 1315 745 500
PasI CC'CwG_GG
Cuts at: 562 2961 562
Size: 2399 4671
PciI A'CATG_T
Cuts at: 3374 6222 3374
Size: 2848 4222
PflMI CCAn_nnn'nTGG
Cuts at: 159 1213 1380 1947 2544 159
Size: 1054 167 567 597 4685
Fragments arranged by size:
4685 1054 597 567 167
PfoI T'CCnGG_A
Cuts at: 1301 1874 1910 2657 1301
Size: 573 36 747 5714
Fragments arranged by size:
5714 747 573 36
PmeI GTTT'AAAC
Cuts at: 4069 4069
Size: 7070
PmlI CAC'GTG
Cuts at: 2286 3720 2286
Size: 1434 5636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5516 5548 5516
Size: 32 7038
PpuMI rG'GwC_Cy
Cuts at: 172 643 693 940 1996 172
Size: 471 50 247 1056 5246
Fragments arranged by size:
5246 1056 471 247 50
PspOMI G'GGCC_C
Cuts at: 336 565 3201 336
Size: 229 2636 4205
Fragments arranged by size:
4205 2636 229
PstI C_TGCA'G
Cuts at: 315 591 1116 1424 2333 2375 2408 2438 3047
Size: 276 525 308 909 42 33 30 609
Cuts at: 3047 3080 3551 3999 4117 4802 315
Size: 33 471 448 118 685 2583
Fragments arranged by size:
2583 909 685 609 525 471 448 308
276 118 42 33 33 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7070
PvuII CAG'CTG
Cuts at: 1173 2328 2433 2742 3148 3576 4495 4855 1173
Size: 1155 105 309 406 428 919 360 3388
Fragments arranged by size:
3388 1155 919 428 406 360 309 105
RsrII CG'GwC_CG
Cuts at: 5265 5265
Size: 7070
SacI G_AGCT'C
Cuts at: 682 1512 3316 4084 682
Size: 830 1804 768 3668
Fragments arranged by size:
3668 1804 830 768
SalI G'TCGA_C
Cuts at: 4107 4107
Size: 7070
SanDI GG'GwC_CC
Cuts at: 643 643
Size: 7070
SapI GCTCTTCn'nnn_
Cuts at: 2255 2931 3098 5093 5303 2255
Size: 676 167 1995 210 4022
Fragments arranged by size:
4022 1995 676 210 167
SbfI CC_TGCA'GG
Cuts at: 4117 4117
Size: 7070
ScaI AGT'ACT
Cuts at: 6587 6587
Size: 7070
SexAI A'CCwGG_T
Cuts at: 230 230
Size: 7070
SfcI C'TryA_G
Cuts at: 33 311 331 573 587 994 1112 1420 1731
Size: 278 20 242 14 407 118 308 311
Cuts at: 1731 2329 2371 2404 2434 3043 3076 3547 3995
Size: 598 42 33 30 609 33 471 448
Cuts at: 3995 4113 4798 5766 5957 33
Size: 118 685 968 191 1146
Fragments arranged by size:
1146 968 685 609 598 471 448 407
311 308 278 242 191 118 118 42
33 33 30 20 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 1454 1454
Size: 7070
SfoI GGC'GCC
Cuts at: 828 4502 4750 828
Size: 3674 248 3148
Fragments arranged by size:
3674 3148 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7070
SgrAI Cr'CCGG_yG
Cuts at: 1886 1886
Size: 7070
SmaI CCC'GGG
Cuts at: 2719 4092 2719
Size: 1373 5697
SmlI C'TyrA_G
Cuts at: 1485 2167 2296 3814 4225 5140 5854 6116 1485
Size: 682 129 1518 411 915 714 262 2439
Fragments arranged by size:
2439 1518 915 714 682 411 262 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7070
SphI G_CATG'C
Cuts at: 4123 4623 4123
Size: 500 6570
StuI AGG'CCT
Cuts at: 344 1071 2075 2811 344
Size: 727 1004 736 4603
Fragments arranged by size:
4603 1004 736 727
StyI C'CwwG_G
Cuts at: 107 158 176 1599 2453 3696 4204 4558 107
Size: 51 18 1423 854 1243 508 354 2619
Fragments arranged by size:
2619 1423 1243 854 508 354 51 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 936 1153 1666 1683 2137 2212 3013 4653 4786
Size: 217 513 17 454 75 801 1640 133
Cuts at: 4786 5245 5454 936
Size: 459 209 2552
Fragments arranged by size:
2552 1640 801 513 459 454 217 209
133 75 17
TatI w'GTAC_w
Cuts at: 3896 3974 6585 3896
Size: 78 2611 4381
Fragments arranged by size:
4381 2611 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 932 964 1224 1256 1527 1559 3080 3112 6587
Size: 32 260 32 271 32 1521 32 3475
Cuts at: 6587 6619 932
Size: 32 1383
Fragments arranged by size:
3475 1521 1383 271 260 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4867 4867
Size: 7070
XbaI T'CTAG_A
Cuts at: 4101 4101
Size: 7070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 136 984 2040 4565 136
Size: 848 1056 2525 2641
Fragments arranged by size:
2641 2525 1056 848
XmaI C'CCGG_G
Cuts at: 2717 4090 2717
Size: 1373 5697
XmnI GAAnn'nnTTC
Cuts at: 1392 1392
Size: 7070
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AleI AscI AvrII BaeI BcgI BfrBI
BmgBI BsaXI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SacII
SnaBI SrfI SspI SwaI XhoI ZraI