Restriction map for
pF1KF0156
(Circular) (Six-base) MAPSORT of: pF1KF0156.seq Check: 4272 from: 1 to: 8687
pF1KF0156 8687 bp
With 182 enzymes: SgfI *
September 4, 2009 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1064 1064
Size: 8687
AatII G_ACGT'C
Cuts at: 4217 5258 4217
Size: 1041 7646
AccI GT'mk_AC
Cuts at: 1304 2416 3443 5725 1304
Size: 1112 1027 2282 4266
Fragments arranged by size:
4266 2282 1112 1027
Acc65I G'GTAC_C
Cuts at: 404 5703 404
Size: 5299 3388
AclI AA'CG_TT
Cuts at: 8527 8527
Size: 8687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 677 762 1225 1372 1495 1730 3721 4037
Size: 188 85 463 147 123 235 1991 316
Cuts at: 4037 4851 4872 5118 6512 6944 7691 7959 489
Size: 814 21 246 1394 432 747 268 1217
Fragments arranged by size:
1991 1394 1217 814 747 463 432 316
268 246 235 188 147 123 85 21
AflIII A'CryG_T
Cuts at: 6039 7143 8120 6039
Size: 1104 977 6606
Fragments arranged by size:
6606 1104 977
AgeI A'CCGG_T
Cuts at: 7075 7075
Size: 8687
AhdI GACnn_n'nnGTC
Cuts at: 1905 1905
Size: 8687
AleI CACnn'nnGTG
Cuts at: 398 1072 4660 5020 5200 5529 398
Size: 674 3588 360 180 329 3556
Fragments arranged by size:
3588 3556 674 360 329 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1090 5547 1090
Size: 4457 4230
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 8655
AlwNI CAG_nnn'CTG
Cuts at: 484 939 1497 1507 1839 2008 2055 2538 2562
Size: 455 558 10 332 169 47 483 24
Cuts at: 2562 4710 4895 5322 5992 7559 484
Size: 2148 185 427 670 1567 1612
Fragments arranged by size:
2148 1612 1567 670 558 483 455 427
332 185 169 47 24 10
ApaI G_GGCC'C
Cuts at: 1364 5350 5389 1364
Size: 3986 39 4662
Fragments arranged by size:
4662 3986 39
ApaLI G'TGCA_C
Cuts at: 1440 7457 1440
Size: 6017 2670
ApoI r'AATT_y
Cuts at: 458 3982 4354 5691 8040 8296 458
Size: 3524 372 1337 2349 256 849
Fragments arranged by size:
3524 2349 1337 849 372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8687
AvaI C'yCGr_G
Cuts at: 2779 5058 5707 2779
Size: 2279 649 5759
Fragments arranged by size:
5759 2279 649
BamHI G'GATC_C
Cuts at: 1153 2939 5712 1153
Size: 1786 2773 4128
Fragments arranged by size:
4128 2773 1786
BanI G'GyrC_C
Cuts at: 404 1406 1450 1516 4578 5263 5703 6117 6365
Size: 1002 44 66 3062 685 440 414 248
Cuts at: 6365 6400 404
Size: 35 2691
Fragments arranged by size:
3062 2691 1002 685 440 414 248 66
44 35
BanII G_rGCy'C
Cuts at: 566 596 873 1364 1605 1653 1808 2402 3088
Size: 30 277 491 241 48 155 594 686
Cuts at: 3088 3960 5039 5350 5389 5572 5701 6731 566
Size: 872 1079 311 39 183 129 1030 2522
Fragments arranged by size:
2522 1079 1030 872 686 594 491 311
277 241 183 155 129 48 39 30
BbeI G_GCGC'C
Cuts at: 6121 6369 6121
Size: 248 8439
BbsI GAAGACnn'nnnn_
Cuts at: 528 2424 2456 2477 2518 3263 3633 4813 5458
Size: 1896 32 21 41 745 370 1180 645
Cuts at: 5458 528
Size: 3757
Fragments arranged by size:
3757 1896 1180 745 645 370 41 32
21
BbvCI CC'TCA_GC
Cuts at: 151 766 1558 1774 1991 2173 2760 3176 4513
Size: 615 792 216 217 182 587 416 1337
Cuts at: 4513 4711 5540 151
Size: 198 829 3298
Fragments arranged by size:
3298 1337 829 792 615 587 416 217
216 198 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5049 5083 5049
Size: 34 8653
BciVI GTATCCnnnnn_n'
Cuts at: 5182 6585 7346 8687 5182
Size: 1403 761 1341 5182
Fragments arranged by size:
5182 1403 1341 761
BclI T'GATC_A
Cuts at: 3120 6207 3120
Size: 3087 5600
BfrBI ATG'CAT
Cuts at: 2346 2869 2346
Size: 523 8164
BglI GCCn_nnn'nGGC
Cuts at: 670 2784 4673 5063 670
Size: 2114 1889 390 4294
Fragments arranged by size:
4294 2114 1889 390
BglII A'GATC_T
Cuts at: 731 854 3605 6202 8239 731
Size: 123 2751 2597 2037 1179
Fragments arranged by size:
2751 2597 2037 1179 123
BlpI GC'TnA_GC
Cuts at: 595 5038 5799 595
Size: 4443 761 3483
Fragments arranged by size:
4443 3483 761
Bme1580I G_kGCm'C
Cuts at: 1364 1444 1527 4583 4981 5350 5389 6312 6405
Size: 80 83 3056 398 369 39 923 93
Cuts at: 6405 7461 1364
Size: 1056 2590
Fragments arranged by size:
3056 2590 1056 923 398 369 93 83
80 39
BmgBI CAC'GTC
Cuts at: 1287 2454 4146 4317 1287
Size: 1167 1692 171 5657
Fragments arranged by size:
5657 1692 1167 171
BmrI ACTGGGnnnn_n'
Cuts at: 1027 2298 3859 5568 5899 5980 6315 8459 1027
Size: 1271 1561 1709 331 81 335 2144 1255
Fragments arranged by size:
2144 1709 1561 1271 1255 335 331 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2166 4209 7054 2166
Size: 2043 2845 3799
Fragments arranged by size:
3799 2845 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 610 961 1411 1600 1720 1806 1853 1867
Size: 388 351 450 189 120 86 47 14
Cuts at: 1867 1879 2140 2584 2621 3042 3622 4179 222
Size: 12 261 444 37 421 580 557 4730
Fragments arranged by size:
4730 580 557 450 444 421 388 351
261 189 120 86 47 37 14 12
Bpu10I CC'TnA_GC
Cuts at: 151 303 766 796 1226 1558 1774 1991 2173
Size: 152 463 30 430 332 216 217 182
Cuts at: 2173 2760 3082 3087 3176 4513 4711 5540 5566
Size: 587 322 5 89 1337 198 829 26
Cuts at: 5566 151
Size: 3272
Fragments arranged by size:
3272 1337 829 587 463 430 332 322
217 216 198 182 152 89 30 26
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1818 2641 5600 5863 6742 7234 7532 811
Size: 1007 823 2959 263 879 492 298 1966
Fragments arranged by size:
2959 1966 1007 879 823 492 298 263
BsaI GGTCTCn'nnnn_
Cuts at: 185 1738 8391 185
Size: 1553 6653 481
Fragments arranged by size:
6653 1553 481
BsaAI yAC'GTr
Cuts at: 4181 5111 5202 6040 4181
Size: 930 91 838 6828
Fragments arranged by size:
6828 930 838 91
BsaBI GATnn'nnATC
Cuts at: 6 5413 6201 8238 6
Size: 5407 788 2037 455
Fragments arranged by size:
5407 2037 788 455
BsaHI Gr'CG_yC
Cuts at: 4214 5255 6118 6366 7068 8564 4214
Size: 1041 863 248 702 1496 4337
Fragments arranged by size:
4337 1496 1041 863 702 248
BsaWI w'CCGG_w
Cuts at: 4091 5876 6397 7075 7349 7496 7907 4091
Size: 1785 521 678 274 147 411 4871
Fragments arranged by size:
4871 1785 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2053 2083 3411 3441 2053
Size: 30 1328 30 7299
Fragments arranged by size:
7299 1328 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1450 1783 2098 2659 4050 4838 1450
Size: 333 315 561 1391 788 5299
Fragments arranged by size:
5299 1391 788 561 333 315
BseYI C'CCAG_C
Cuts at: 498 971 1432 1590 2228 2964 3212 3228 3694
Size: 473 461 158 638 736 248 16 466
Cuts at: 3694 3832 4608 4871 5388 6112 6870 7447 7943
Size: 138 776 263 517 724 758 577 496
Cuts at: 7943 498
Size: 1242
Fragments arranged by size:
1242 776 758 736 724 638 577 517
496 473 466 461 263 248 158 138
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1169 2021 2539 3192 3232 3370 3519 4339
Size: 838 852 518 653 40 138 149 820
Cuts at: 4339 4351 5148 331
Size: 12 797 3870
Fragments arranged by size:
3870 852 838 820 797 653 518 149
138 40 12
BsiEI CG_ry'CG
Cuts at: 104 6229 6275 7483 104
Size: 6125 46 1208 1308
Fragments arranged by size:
6125 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 566 596 1444 1808 1856 2126 2288 3088 4180
Size: 30 848 364 48 270 162 800 1092
Cuts at: 4180 5039 5289 5572 5701 6479 6669 7461 566
Size: 859 250 283 129 778 190 792 1792
Fragments arranged by size:
1792 1092 859 848 800 792 778 364
283 270 250 190 162 129 48 30
BsmI GAATG_Cn'
Cuts at: 657 2251 657
Size: 1594 7093
BsmBI CGTCTCn'nnnn_
Cuts at: 1320 1970 2641 4307 5262 1320
Size: 650 671 1666 955 4745
Fragments arranged by size:
4745 1666 955 671 650
Bsp1286I G_dGCh'C
Cuts at: 566 596 873 1364 1444 1527 1605 1653 1808
Size: 30 277 491 80 83 78 48 155
Cuts at: 1808 1856 2126 2288 2402 3088 3960 4180 4583
Size: 48 270 162 114 686 872 220 403
Cuts at: 4583 4981 5039 5289 5350 5389 5572 5701 6312
Size: 398 58 250 61 39 183 129 611
Cuts at: 6312 6405 6479 6669 6731 7461 566
Size: 93 74 190 62 730 1792
Fragments arranged by size:
1792 872 730 686 611 491 403 398
277 270 250 220 190 183 162 155
129 114 93 83 80 78 74 62
61 58 48 48 39 30
BspEI T'CCGG_A
Cuts at: 4091 7907 4091
Size: 3816 4871
BspHI T'CATG_A
Cuts at: 7863 7863
Size: 8687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1064 1492 3850 4253 5737 5951 6253 6634 1064
Size: 428 2358 403 1484 214 302 381 3117
Fragments arranged by size:
3117 2358 1484 428 403 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2637 5150 6979 7033 8517 8682 50
Size: 2587 2513 1829 54 1484 165 55
Fragments arranged by size:
2587 2513 1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2346 3852 6599 2346
Size: 1506 2747 4434
Fragments arranged by size:
4434 2747 1506
BsrFI r'CCGG_y
Cuts at: 6685 6866 7075 6685
Size: 181 209 8297
Fragments arranged by size:
8297 209 181
BsrGI T'GTAC_A
Cuts at: 818 2069 818
Size: 1251 7436
BssSI C'ACGA_G
Cuts at: 3528 6958 7316 3528
Size: 3430 358 4899
Fragments arranged by size:
4899 3430 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1839 2008 4465 6064 1839
Size: 169 2457 1599 4462
Fragments arranged by size:
4462 2457 1599 169
BstBI TT'CG_AA
Cuts at: 7048 7048
Size: 8687
BstXI CCAn_nnnn'nTGG
Cuts at: 258 288 907 1077 4074 5019 258
Size: 30 619 170 2997 945 3926
Fragments arranged by size:
3926 2997 945 619 170 30
BstYI r'GATC_y
Cuts at: 1 731 854 1153 2939 3605 3936 5181 5712
Size: 730 123 299 1786 666 331 1245 531
Cuts at: 5712 6179 6202 6537 6783 7795 7881 7893 8239
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8239 1
Size: 449
Fragments arranged by size:
1786 1245 1012 730 666 531 467 449
346 335 331 299 246 123 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 427 1697 427
Size: 1270 7417
BtgI C'CryG_G
Cuts at: 3007 3431 3827 3950 5662 3007
Size: 424 396 123 1712 6032
Fragments arranged by size:
6032 1712 424 396 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 3579 6637 6818 6981 1981
Size: 1598 3058 181 163 3687
Fragments arranged by size:
3687 3058 1598 181 163
BtsI GCAGTG_nn'
Cuts at: 1219 1840 3456 4466 5930 1219
Size: 621 1616 1010 1464 3976
Fragments arranged by size:
3976 1616 1464 1010 621
ClaI AT'CG_AT
Cuts at: 2582 2582
Size: 8687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 726 761 726
Size: 35 8652
DraI TTT'AAA
Cuts at: 2195 3672 3999 5686 2195
Size: 1477 327 1687 5196
Fragments arranged by size:
5196 1687 1477 327
DraIII CAC_nnn'GTG
Cuts at: 2741 3363 4170 2741
Size: 622 807 7258
Fragments arranged by size:
7258 807 622
DrdI GACnn_nn'nnGTC
Cuts at: 4030 5437 6393 7251 4030
Size: 1407 956 858 5466
Fragments arranged by size:
5466 1407 956 858
EaeI y'GGCC_r
Cuts at: 165 1538 1625 3387 4064 4674 4785 5324 5665
Size: 1373 87 1762 677 610 111 539 341
Cuts at: 5665 6272 6446 6837 6864 8209 165
Size: 607 174 391 27 1345 643
Fragments arranged by size:
1762 1373 1345 677 643 610 607 539
391 341 174 111 87 27
EagI C'GGCC_G
Cuts at: 6272 6272
Size: 8687
EarI CTCTTCn'nnn_
Cuts at: 995 1201 2116 2157 2618 2844 4168 5494 6710
Size: 206 915 41 461 226 1324 1326 1216
Cuts at: 6710 6920 995
Size: 210 2762
Fragments arranged by size:
2762 1326 1324 1216 915 461 226 210
206 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4148 6895 7205 7351 8497 4148
Size: 2747 310 146 1146 4338
Fragments arranged by size:
4338 2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 564 594 1806 3086 5037 5570 5699 564
Size: 30 1212 1280 1951 533 129 3552
Fragments arranged by size:
3552 1951 1280 1212 533 129 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 489 610 677 762 961 1225 1372 1411
Size: 267 121 67 85 199 264 147 39
Cuts at: 1411 1495 1600 1720 1730 1806 1853 1867 1879
Size: 84 105 120 10 76 47 14 12
Cuts at: 1879 2140 2584 2621 3042 3622 3721 4037 4179
Size: 261 444 37 421 580 99 316 142
Cuts at: 4179 4851 4872 5118 6512 6944 7691 7959 222
Size: 672 21 246 1394 432 747 268 950
Fragments arranged by size:
1394 950 747 672 580 444 432 421
316 268 267 264 261 246 199 147
142 121 120 105 99 85 84 76
67 47 39 37 21 14 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2934 3586 5077 5380 2934
Size: 652 1491 303 6241
Fragments arranged by size:
6241 1491 652 303
EcoO109I rG'GnC_Cy
Cuts at: 643 1021 1587 1914 2796 3078 3375 3879 4344
Size: 378 566 327 882 282 297 504 465
Cuts at: 4344 4377 4715 4862 4923 5346 5385 5562 5595
Size: 33 338 147 61 423 39 177 33
Cuts at: 5595 5624 5826 643
Size: 29 202 3504
Fragments arranged by size:
3504 882 566 504 465 423 378 338
327 297 282 202 177 147 61 39
33 33 29
EcoRI G'AATT_C
Cuts at: 5691 5691
Size: 8687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 711 1390 1917 2224 2452 3322 6706 711
Size: 679 527 307 228 870 3384 2692
Fragments arranged by size:
3384 2692 870 679 527 307 228
FspI TGC'GCA
Cuts at: 6468 6468
Size: 8687
HaeII r_GCGC'y
Cuts at: 6121 6369 7391 8370 6121
Size: 248 1022 979 6438
Fragments arranged by size:
6438 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1923 2677 2709 2809 2841 3511 3543 3880
Size: 32 754 32 100 32 670 32 337
Cuts at: 3880 3912 6529 6559 6561 6591 1891
Size: 32 2617 30 2 30 3987
Fragments arranged by size:
3987 2617 754 670 337 100 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 5726 5726
Size: 8687
HindIII A'AGCT_T
Cuts at: 3675 4137 3675
Size: 462 8225
KasI G'GCGC_C
Cuts at: 6117 6365 6117
Size: 248 8439
KpnI G_GTAC'C
Cuts at: 408 5707 408
Size: 5299 3388
MluI A'CGCG_T
Cuts at: 8120 8120
Size: 8687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2520 3491 3714 4940 7358 7542 2342
Size: 178 971 223 1226 2418 184 3487
Fragments arranged by size:
3487 2418 1226 971 223 184 178
MscI TGG'CCA
Cuts at: 167 1627 3389 4676 4787 5326 6448 167
Size: 1460 1762 1287 111 539 1122 2406
Fragments arranged by size:
2406 1762 1460 1287 1122 539 111
MslI CAynn'nnrTG
Cuts at: 139 398 1058 1072 2131 2291 4072 4660 5020
Size: 259 660 14 1059 160 1781 588 360
Cuts at: 5020 5200 5529 6676 6803 139
Size: 180 329 1147 127 2023
Fragments arranged by size:
2023 1781 1147 1059 660 588 360 329
259 180 160 127 14
MspA1I CmG'CkG
Cuts at: 468 765 1708 2538 3216 3829 4871 4892 4961
Size: 297 943 830 678 613 1042 21 69
Cuts at: 4961 5798 6112 6472 7485 7730 468
Size: 837 314 360 1013 245 1425
Fragments arranged by size:
1425 1042 1013 943 837 830 678 613
360 314 297 245 69 21
NaeI GCC'GGC
Cuts at: 6868 6868
Size: 8687
NarI GG'CG_CC
Cuts at: 6118 6366 6118
Size: 248 8439
NcoI C'CATG_G
Cuts at: 3007 3950 5662 3007
Size: 943 1712 6032
Fragments arranged by size:
6032 1712 943
NgoMIV G'CCGG_C
Cuts at: 6866 6866
Size: 8687
NsiI A_TGCA'T
Cuts at: 2348 2871 2348
Size: 523 8164
NspI r_CATG'y
Cuts at: 1063 3558 3858 4293 5740 6240 7147 1063
Size: 2495 300 435 1447 500 907 2603
Fragments arranged by size:
2603 2495 1447 907 500 435 300
PasI CC'CwG_GG
Cuts at: 1644 3875 4331 5592 1644
Size: 2231 456 1261 4739
Fragments arranged by size:
4739 2231 1261 456
PciI A'CATG_T
Cuts at: 7143 7143
Size: 8687
PflMI CCAn_nnn'nTGG
Cuts at: 414 615 939 1497 1623 1835 2055 2930 5342
Size: 201 324 558 126 212 220 875 2412
Cuts at: 5342 414
Size: 3759
Fragments arranged by size:
3759 2412 875 558 324 220 212 201
126
PfoI T'CCnGG_A
Cuts at: 1463 1856 2117 2942 3932 1463
Size: 393 261 825 990 6218
Fragments arranged by size:
6218 990 825 393 261
PmeI GTTT'AAAC
Cuts at: 5686 5686
Size: 8687
PmlI CAC'GTG
Cuts at: 4181 5111 5202 4181
Size: 930 91 7666
Fragments arranged by size:
7666 930 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7826 7858 7826
Size: 32 8655
PpuMI rG'GwC_Cy
Cuts at: 643 1587 1914 2796 3375 3879 4344 4862 4923
Size: 944 327 882 579 504 465 518 61
Cuts at: 4923 643
Size: 4407
Fragments arranged by size:
4407 944 882 579 518 504 465 327
61
PspOMI G'GGCC_C
Cuts at: 1360 5346 5385 1360
Size: 3986 39 4662
Fragments arranged by size:
4662 3986 39
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3853 3885 3853
Size: 32 8655
PstI C_TGCA'G
Cuts at: 582 1049 1334 1403 1553 2282 3590 3773 4130
Size: 467 285 69 150 729 1308 183 357
Cuts at: 4130 4623 4706 5489 5649 5734 6419 582
Size: 493 83 783 160 85 685 2850
Fragments arranged by size:
2850 1308 783 729 685 493 467 357
285 183 160 150 85 83 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8687
PvuII CAG'CTG
Cuts at: 468 765 1708 2538 3216 4871 4892 6112 6472
Size: 297 943 830 678 1655 21 1220 360
Cuts at: 6472 468
Size: 2683
Fragments arranged by size:
2683 1655 1220 943 830 678 360 297
21
RsrII CG'GwC_CG
Cuts at: 6882 6882
Size: 8687
SacI G_AGCT'C
Cuts at: 566 596 1808 3088 5039 5572 5701 566
Size: 30 1212 1280 1951 533 129 3552
Fragments arranged by size:
3552 1951 1280 1212 533 129 30
SacII CC_GC'GG
Cuts at: 3830 3830
Size: 8687
SalI G'TCGA_C
Cuts at: 5724 5724
Size: 8687
SanDI GG'GwC_CC
Cuts at: 4344 4344
Size: 8687
SapI GCTCTTCn'nnn_
Cuts at: 1201 4168 6710 6920 1201
Size: 2967 2542 210 2968
Fragments arranged by size:
2968 2967 2542 210
SbfI CC_TGCA'GG
Cuts at: 1049 3590 5734 1049
Size: 2541 2144 4002
Fragments arranged by size:
4002 2541 2144
ScaI AGT'ACT
Cuts at: 8204 8204
Size: 8687
SexAI A'CCwGG_T
Cuts at: 3359 4085 3359
Size: 726 7961
SfcI C'TryA_G
Cuts at: 33 578 1045 1330 1399 1549 2278 2841 3586
Size: 545 467 285 69 150 729 563 745
Cuts at: 3586 3769 4126 4619 4695 4702 4820 5485 5577
Size: 183 357 493 76 7 118 665 92
Cuts at: 5577 5645 5730 6415 7408 7599 33
Size: 68 85 685 993 191 1121
Fragments arranged by size:
1121 993 745 729 685 665 563 545
493 467 357 285 191 183 150 118
92 85 76 69 68 7
SfoI GGC'GCC
Cuts at: 6119 6367 6119
Size: 248 8439
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8687
SmaI CCC'GGG
Cuts at: 2781 5709 2781
Size: 2928 5759
SmlI C'TyrA_G
Cuts at: 826 1797 2656 5615 5842 6757 7249 7511 826
Size: 971 859 2959 227 915 492 262 2002
Fragments arranged by size:
2959 2002 971 915 859 492 262 227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8687
SphI G_CATG'C
Cuts at: 1063 4293 5740 6240 1063
Size: 3230 1447 500 3510
Fragments arranged by size:
3510 3230 1447 500
SrfI GCCC'GGGC
Cuts at: 2781 2781
Size: 8687
StuI AGG'CCT
Cuts at: 439 2662 3667 4114 5083 439
Size: 2223 1005 447 969 4043
Fragments arranged by size:
4043 2223 1005 969 447
StyI C'CwwG_G
Cuts at: 634 1622 1748 2792 3007 3950 3965 5662 5821
Size: 988 126 1044 215 943 15 1697 159
Cuts at: 5821 6175 634
Size: 354 3146
Fragments arranged by size:
3146 1697 1044 988 943 354 215 159
126 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 799 1149 2062 3226 4066 4123 4622 4694
Size: 457 350 913 1164 840 57 499 72
Cuts at: 4694 5212 6270 6403 6862 7071 342
Size: 518 1058 133 459 209 1958
Fragments arranged by size:
1958 1164 1058 913 840 518 499 459
457 350 209 133 72 57
TatI w'GTAC_w
Cuts at: 818 1097 2069 8202 818
Size: 279 972 6133 1303
Fragments arranged by size:
6133 1303 972 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1145 1177 1517 1549 1579 1611 1677 1709 2788
Size: 32 340 32 30 32 66 32 1079
Cuts at: 2788 2820 3411 3443 3448 3480 8204 8236 1145
Size: 32 591 32 5 32 4724 32 1596
Fragments arranged by size:
4724 1596 1079 591 340 66 32 32
32 32 32 32 32 32 30 5
Tth111I GACn'n_nGTC
Cuts at: 2167 4393 4921 6484 2167
Size: 2226 528 1563 4370
Fragments arranged by size:
4370 2226 1563 528
XbaI T'CTAG_A
Cuts at: 5718 5718
Size: 8687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1660 1948 3872 5542 6182 1660
Size: 288 1924 1670 640 4165
Fragments arranged by size:
4165 1924 1670 640 288
XmaI C'CCGG_G
Cuts at: 2779 5707 2779
Size: 2928 5759
XmnI GAAnn'nnTTC
Cuts at: 623 3928 623
Size: 3305 5382
ZraI GAC'GTC
Cuts at: 4215 5256 4215
Size: 1041 7646
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BmtI BsiWI BssHII
BstEII BstZ17I EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PshAI PsiI PspXI SfiI
SgrAI SnaBI SspI SwaI XhoI