Restriction map for
pF1KF0175
(Circular) (Six-base) MAPSORT of: pF1KF0175.seq Check: 489 from: 1 to: 9470
pF1KF0175 9470 bp
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 459 459
Size: 9470
AatII G_ACGT'C
Cuts at: 2882 2882
Size: 9470
AccI GT'mk_AC
Cuts at: 1064 5216 5585 6508 1064
Size: 4152 369 923 4026
Fragments arranged by size:
4152 4026 923 369
Acc65I G'GTAC_C
Cuts at: 6486 6486
Size: 9470
AclI AA'CG_TT
Cuts at: 9310 9310
Size: 9470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1372 2269 2455 4750 5473 6444 7295 7727
Size: 74 897 186 2295 723 971 851 432
Cuts at: 7727 8080 8742 1298
Size: 353 662 2026
Fragments arranged by size:
2295 2026 971 897 851 723 662 432
353 186 74
AfeI AGC'GCT
Cuts at: 4145 4145
Size: 9470
AflIII A'CryG_T
Cuts at: 3632 6822 8622 8903 3632
Size: 3190 1800 281 4199
Fragments arranged by size:
4199 3190 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4584 4584
Size: 9470
AleI CACnn'nnGTG
Cuts at: 3082 3082
Size: 9470
AlwNI CAG_nnn'CTG
Cuts at: 1180 2331 2379 3814 5502 6775 8213 1180
Size: 1151 48 1435 1688 1273 1438 2437
Fragments arranged by size:
2437 1688 1438 1435 1273 1151 48
ApaI G_GGCC'C
Cuts at: 1721 3507 5458 1721
Size: 1786 1951 5733
Fragments arranged by size:
5733 1951 1786
ApaLI G'TGCA_C
Cuts at: 8308 8308
Size: 9470
ApoI r'AATT_y
Cuts at: 4663 4855 4874 6474 8823 9079 4663
Size: 192 19 1600 2349 256 5054
Fragments arranged by size:
5054 2349 1600 256 192 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 9470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9470
AvaI C'yCGr_G
Cuts at: 741 796 1096 3403 3644 6490 741
Size: 55 300 2307 241 2846 3721
Fragments arranged by size:
3721 2846 2307 300 241 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5353 5386 5353
Size: 33 9437
BamHI G'GATC_C
Cuts at: 962 4052 5435 6495 962
Size: 3090 1383 1060 3937
Fragments arranged by size:
3937 3090 1383 1060
BanI G'GyrC_C
Cuts at: 890 1120 2093 2586 2620 2688 2695 3443 5361
Size: 230 973 493 34 68 7 748 1918
Cuts at: 5361 6281 6486 6900 7148 7183 890
Size: 920 205 414 248 35 3177
Fragments arranged by size:
3177 1918 973 920 748 493 414 248
230 205 68 35 34 7
BanII G_rGCy'C
Cuts at: 298 533 750 1046 1139 1341 1721 1787 2309
Size: 235 217 296 93 202 380 66 522
Cuts at: 2309 2456 2519 2741 3507 3989 4349 4556 5099
Size: 147 63 222 766 482 360 207 543
Cuts at: 5099 5253 5458 5995 6251 6484 7514 298
Size: 154 205 537 256 233 1030 2254
Fragments arranged by size:
2254 1030 766 543 537 522 482 380
360 296 256 235 233 222 217 207
205 202 154 147 93 66 63
BbeI G_GCGC'C
Cuts at: 894 2699 3447 6285 6904 7152 894
Size: 1805 748 2838 619 248 3212
Fragments arranged by size:
3212 2838 1805 748 619 248
BbsI GAAGACnn'nnnn_
Cuts at: 683 1286 1350 1516 2974 3475 3727 4529 4898
Size: 603 64 166 1458 501 252 802 369
Cuts at: 4898 683
Size: 5255
Fragments arranged by size:
5255 1458 802 603 501 369 252 166
64
BbvCI CC'TCA_GC
Cuts at: 2774 2774
Size: 9470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 9436
BciVI GTATCCnnnnn_n'
Cuts at: 7368 8424 9470 7368
Size: 1056 1046 7368
Fragments arranged by size:
7368 1056 1046
BclI T'GATC_A
Cuts at: 6990 6990
Size: 9470
BglI GCCn_nnn'nGGC
Cuts at: 2251 3131 4394 2251
Size: 880 1263 7327
Fragments arranged by size:
7327 1263 880
BglII A'GATC_T
Cuts at: 1415 1499 3788 5036 5951 6985 9022 1415
Size: 84 2289 1248 915 1034 2037 1863
Fragments arranged by size:
2289 2037 1863 1248 1034 915 84
BlpI GC'TnA_GC
Cuts at: 2291 3815 5864 6582 2291
Size: 1524 2049 718 5179
Fragments arranged by size:
5179 2049 1524 718
Bme1580I G_kGCm'C
Cuts at: 503 1123 1721 2505 2591 2848 3127 3507 5458
Size: 620 598 784 86 257 279 380 1951
Cuts at: 5458 7095 7188 8312 503
Size: 1637 93 1124 1661
Fragments arranged by size:
1951 1661 1637 1124 784 620 598 380
279 257 93 86
BmgBI CAC'GTC
Cuts at: 778 1116 2274 4527 5790 778
Size: 338 1158 2253 1263 4458
Fragments arranged by size:
4458 2253 1263 1158 338
BmrI ACTGGGnnnn_n'
Cuts at: 140 1608 2163 2850 3139 3249 5452 6682 6763
Size: 1468 555 687 289 110 2203 1230 81
Cuts at: 6763 7098 9242 140
Size: 335 2144 368
Fragments arranged by size:
2203 2144 1468 1230 687 555 368 335
289 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 400 2370 3393 7837 400
Size: 1970 1023 4444 2033
Fragments arranged by size:
4444 2033 1970 1023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 547 722 1279 1688 1861 2248 2488 2963
Size: 35 175 557 409 173 387 240 475
Cuts at: 2963 3484 4132 4456 4729 4771 5113 5161 5242
Size: 521 648 324 273 42 342 48 81
Cuts at: 5242 5850 5922 512
Size: 608 72 4060
Fragments arranged by size:
4060 648 608 557 521 475 409 387
342 324 273 240 175 173 81 72
48 42 35
Bpu10I CC'TnA_GC
Cuts at: 2404 2774 3001 5707 6263 2404
Size: 370 227 2706 556 5611
Fragments arranged by size:
5611 2706 556 370 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 3517 3579 6289 6646 7525 8239 8537 256
Size: 3261 62 2710 357 879 714 298 1189
Fragments arranged by size:
3261 2710 1189 879 714 357 298 62
BsaI GGTCTCn'nnnn_
Cuts at: 1837 9174 1837
Size: 7337 2133
BsaAI yAC'GTr
Cuts at: 5517 6823 5517
Size: 1306 8164
BsaBI GATnn'nnATC
Cuts at: 6 1588 3787 6984 9021 6
Size: 1582 2199 3197 2037 455
Fragments arranged by size:
3197 2199 2037 1582 455
BsaHI Gr'CG_yC
Cuts at: 891 1988 2696 2879 2990 3444 6282 6901 7149
Size: 1097 708 183 111 454 2838 619 248
Cuts at: 7149 7851 9347 891
Size: 702 1496 1014
Fragments arranged by size:
2838 1496 1097 1014 708 702 619 454
248 183 111
BsaWI w'CCGG_w
Cuts at: 1106 2961 4283 5372 6353 6659 7180 7858 8269
Size: 1855 1322 1089 981 306 521 678 411
Cuts at: 8269 8416 8690 1106
Size: 147 274 1886
Fragments arranged by size:
1886 1855 1322 1089 981 678 521 411
306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4204 4234 4599 4629 5167 5197 5956 5986 4204
Size: 30 365 30 538 30 759 30 7688
Fragments arranged by size:
7688 759 538 365 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 512 683 1084 1349 1685 2527 4209 4357 4607
Size: 171 401 265 336 842 1682 148 250
Cuts at: 4607 4840 5170 5317 5479 6232 512
Size: 233 330 147 162 753 3750
Fragments arranged by size:
3750 1682 842 753 401 336 330 265
250 233 171 162 148 147
BseYI C'CCAG_C
Cuts at: 1195 1233 3194 3284 3365 3826 4225 4896 5321
Size: 38 1961 90 81 461 399 671 425
Cuts at: 5321 5931 6895 7653 8318 8726 1195
Size: 610 964 758 665 408 1939
Fragments arranged by size:
1961 1939 964 758 671 665 610 461
425 408 399 90 81 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1512 2099 2731 3691 3887 4057 4279 4293 5070
Size: 587 632 960 196 170 222 14 777
Cuts at: 5070 5112 1512
Size: 42 5870
Fragments arranged by size:
5870 960 777 632 587 222 196 170
42 14
BsiEI CG_ry'CG
Cuts at: 104 2927 5213 7012 7058 8288 104
Size: 2823 2286 1799 46 1230 1286
Fragments arranged by size:
2823 2286 1799 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1787 1862 2263 2309 2456 3989 4349 4532 5516
Size: 75 401 46 147 1533 360 183 984
Cuts at: 5516 6052 6484 7262 7452 8312 1787
Size: 536 432 778 190 860 2945
Fragments arranged by size:
2945 1533 984 860 778 536 432 401
360 190 183 147 75 46
BsmBI CGTCTCn'nnnn_
Cuts at: 322 3555 3702 5200 322
Size: 3233 147 1498 4592
Fragments arranged by size:
4592 3233 1498 147
Bsp1286I G_dGCh'C
Cuts at: 298 503 533 750 1046 1123 1139 1341 1721
Size: 205 30 217 296 77 16 202 380
Cuts at: 1721 1787 1862 2263 2309 2456 2505 2519 2591
Size: 66 75 401 46 147 49 14 72
Cuts at: 2591 2741 2848 3127 3507 3989 4349 4532 4556
Size: 150 107 279 380 482 360 183 24
Cuts at: 4556 5099 5253 5458 5516 5995 6052 6251 6484
Size: 543 154 205 58 479 57 199 233
Cuts at: 6484 7095 7188 7262 7452 7514 8312 298
Size: 611 93 74 190 62 798 1456
Fragments arranged by size:
1456 798 611 543 482 479 401 380
380 360 296 279 233 217 205 205
202 199 190 183 154 150 147 107
93 77 75 74 72 66 62 58
57 49 46 30 24 16 14
BspEI T'CCGG_A
Cuts at: 1106 2961 4283 1106
Size: 1855 1322 6293
Fragments arranged by size:
6293 1855 1322
BspHI T'CATG_A
Cuts at: 4632 7902 4632
Size: 3270 6200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 459 1313 2050 2223 4088 5134 5864 6520 6734
Size: 854 737 173 1865 1046 730 656 214
Cuts at: 6734 7036 7417 459
Size: 302 381 2512
Fragments arranged by size:
2512 1865 1046 854 737 730 656 381
302 214 173
BsrBI CCG'CTC
Cuts at: 50 3225 4430 6222 7762 7816 9300 9465 50
Size: 3175 1205 1792 1540 54 1484 165 55
Fragments arranged by size:
3175 1792 1540 1484 1205 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5879 7382 5879
Size: 1503 7967
BsrFI r'CCGG_y
Cuts at: 161 648 1823 2210 2926 2992 3801 4005 4400
Size: 487 1175 387 716 66 809 204 395
Cuts at: 4400 7468 7649 161
Size: 3068 181 1982
Fragments arranged by size:
3068 1982 1175 809 716 487 395 387
204 181 66
BssSI C'ACGA_G
Cuts at: 624 4231 5936 7741 8449 624
Size: 3607 1705 1805 708 1645
Fragments arranged by size:
3607 1805 1705 1645 708
BstAPI GCAn_nnn'nTGC
Cuts at: 6847 6847
Size: 9470
BstBI TT'CG_AA
Cuts at: 7831 7831
Size: 9470
BstEII G'GTnAC_C
Cuts at: 930 2995 930
Size: 2065 7405
BstXI CCAn_nnnn'nTGG
Cuts at: 1854 2197 2367 3134 3834 4024 4233 6447 1854
Size: 343 170 767 700 190 209 2214 4877
Fragments arranged by size:
4877 2214 767 700 343 209 190 170
BstYI r'GATC_y
Cuts at: 1 962 1415 1499 3056 3306 3788 4052 4836
Size: 961 453 84 1557 250 482 264 784
Cuts at: 4836 5036 5435 5951 6495 6962 6985 7320 7566
Size: 200 399 516 544 467 23 335 246
Cuts at: 7566 7872 7884 7970 9022 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1557 1052 961 784 544 516 482 467
453 449 399 335 306 264 250 246
200 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 1921 1921
Size: 9470
BtgI C'CryG_G
Cuts at: 617 656 2264 3919 5638 617
Size: 39 1608 1655 1719 4449
Fragments arranged by size:
4449 1719 1655 1608 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2295 3673 7420 7601 7764 2295
Size: 1378 3747 181 163 4001
Fragments arranged by size:
4001 3747 1378 181 163
BtsI GCAGTG_nn'
Cuts at: 1543 2263 6713 1543
Size: 720 4450 4300
Fragments arranged by size:
4450 4300 720
ClaI AT'CG_AT
Cuts at: 4379 4649 4379
Size: 270 9200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2613 2625 2648 2660 2613
Size: 12 23 12 9423
Fragments arranged by size:
9423 23 12 12
DraI TTT'AAA
Cuts at: 6469 6469
Size: 9470
DraIII CAC_nnn'GTG
Cuts at: 4486 4486
Size: 9470
DrdI GACnn_nn'nnGTC
Cuts at: 4260 7176 8520 4260
Size: 2916 1344 5210
Fragments arranged by size:
5210 2916 1344
EaeI y'GGCC_r
Cuts at: 646 1514 1821 2442 2924 3803 4007 4208 4395
Size: 868 307 621 482 879 204 201 187
Cuts at: 4395 4934 6231 6404 7055 7229 7620 7647 8992
Size: 539 1297 173 651 174 391 27 1345
Cuts at: 8992 646
Size: 1124
Fragments arranged by size:
1345 1297 1124 879 868 651 621 539
482 391 307 204 201 187 174 173
27
EagI C'GGCC_G
Cuts at: 2924 7055 2924
Size: 4131 5339
EarI CTCTTCn'nnn_
Cuts at: 202 991 2611 3008 4189 4321 4634 4873 5341
Size: 789 1620 397 1181 132 313 239 468
Cuts at: 5341 5411 6140 6376 6388 7493 7703 202
Size: 70 729 236 12 1105 210 1969
Fragments arranged by size:
1969 1620 1181 1105 789 729 468 397
313 239 236 210 132 70 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 393 727 773 2491 2905 3361 3367 3418 4480
Size: 334 46 1718 414 456 6 51 1062
Cuts at: 4480 5302 7678 8420 8566 9280 393
Size: 822 2376 742 146 714 583
Fragments arranged by size:
2376 1718 1062 822 742 714 583 456
414 334 146 51 46 6
EcoICRI GAG'CTC
Cuts at: 1785 2307 2454 3987 4347 6482 1785
Size: 522 147 1533 360 2135 4773
Fragments arranged by size:
4773 2135 1533 522 360 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 547 722 1279 1298 1372 1688 1861 2248
Size: 35 175 557 19 74 316 173 387
Cuts at: 2248 2269 2455 2488 2963 3484 4132 4456 4729
Size: 21 186 33 475 521 648 324 273
Cuts at: 4729 4750 4771 5113 5161 5242 5473 5850 5922
Size: 21 21 342 48 81 231 377 72
Cuts at: 5922 6444 7295 7727 8080 8742 512
Size: 522 851 432 353 662 1240
Fragments arranged by size:
1240 851 662 648 557 522 521 475
432 387 377 353 342 324 316 273
231 186 175 173 81 74 72 48
35 33 21 21 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 289 1152 2230 2509 4711 5185 5901 289
Size: 863 1078 279 2202 474 716 3858
Fragments arranged by size:
3858 2202 1078 863 716 474 279
EcoO109I rG'GnC_Cy
Cuts at: 267 366 381 452 1237 1718 1881 3243 3475
Size: 99 15 71 785 481 163 1362 232
Cuts at: 3475 3503 3504 3648 4179 4773 4863 4945 5004
Size: 28 1 144 531 594 90 82 59
Cuts at: 5004 5148 5499 5601 6273 6609 267
Size: 144 351 102 672 336 3128
Fragments arranged by size:
3128 1362 785 672 594 531 481 351
336 232 163 144 144 102 99 90
82 71 59 28 15 1
EcoRI G'AATT_C
Cuts at: 6474 6474
Size: 9470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 206 1461 2928 4884 5913 5991 6069 7489 206
Size: 1255 1467 1956 1029 78 78 1420 2187
Fragments arranged by size:
2187 1956 1467 1420 1255 1029 78 78
FseI GG_CCGG'CC
Cuts at: 652 652
Size: 9470
FspI TGC'GCA
Cuts at: 2392 3298 7251 2392
Size: 906 3953 4611
Fragments arranged by size:
4611 3953 906
HaeII r_GCGC'y
Cuts at: 894 1255 2699 3285 3447 4147 6285 6904 7152
Size: 361 1444 586 162 700 2138 619 248
Cuts at: 7152 8382 9153 894
Size: 1230 771 1211
Fragments arranged by size:
2138 1444 1230 1211 771 700 619 586
361 248 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 580 612 1074 1106 1174 1206 2263
Size: 32 268 32 462 32 68 32 1057
Cuts at: 2263 2295 3142 3174 3529 3561 3655 3687 4444
Size: 32 847 32 355 32 94 32 757
Cuts at: 4444 4459 4476 4491 4570 4602 4762 4794 4821
Size: 15 17 15 79 32 160 32 27
Cuts at: 4821 4853 5115 5147 5167 5199 5678 5710 5956
Size: 32 262 32 20 32 479 32 246
Cuts at: 5956 5988 7312 7342 7344 7374 280
Size: 32 1324 30 2 30 2376
Fragments arranged by size:
2376 1324 1057 847 757 479 462 355
268 262 246 160 94 79 68 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
27 20 17 15 15 2
HincII GTy'rAC
Cuts at: 3898 5217 5586 6509 3898
Size: 1319 369 923 6859
Fragments arranged by size:
6859 1319 923 369
HindIII A'AGCT_T
Cuts at: 6265 6265
Size: 9470
KasI G'GCGC_C
Cuts at: 890 2695 3443 6281 6900 7148 890
Size: 1805 748 2838 619 248 3212
Fragments arranged by size:
3212 2838 1805 748 619 248
KpnI G_GTAC'C
Cuts at: 6490 6490
Size: 9470
MluI A'CGCG_T
Cuts at: 8903 8903
Size: 9470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5731 8229 8413 5731
Size: 2498 184 6788
Fragments arranged by size:
6788 2498 184
MscI TGG'CCA
Cuts at: 4210 6233 6406 7231 4210
Size: 2023 173 825 6449
Fragments arranged by size:
6449 2023 825 173
MslI CAynn'nnrTG
Cuts at: 2548 3082 3835 4117 4324 4906 5740 7459 7586
Size: 534 753 282 207 582 834 1719 127
Cuts at: 7586 2548
Size: 4432
Fragments arranged by size:
4432 1719 834 753 582 534 282 207
127
MspA1I CmG'CkG
Cuts at: 486 897 937 2052 2193 2295 2388 2583 3440
Size: 411 40 1115 141 102 93 195 857
Cuts at: 3440 3484 4206 4749 4932 5863 6063 6581 6895
Size: 44 722 543 183 931 200 518 314
Cuts at: 6895 7255 8039 8284 486
Size: 360 784 245 1672
Fragments arranged by size:
1672 1115 931 857 784 722 543 518
411 360 314 245 200 195 183 141
102 93 44 40
NaeI GCC'GGC
Cuts at: 650 1825 2928 3803 4007 4402 7651 650
Size: 1175 1103 875 204 395 3249 2469
Fragments arranged by size:
3249 2469 1175 1103 875 395 204
NarI GG'CG_CC
Cuts at: 891 2696 3444 6282 6901 7149 891
Size: 1805 748 2838 619 248 3212
Fragments arranged by size:
3212 2838 1805 748 619 248
NcoI C'CATG_G
Cuts at: 617 617
Size: 9470
NgoMIV G'CCGG_C
Cuts at: 648 1823 2926 3801 4005 4400 7649 648
Size: 1175 1103 875 204 395 3249 2469
Fragments arranged by size:
3249 2469 1175 1103 875 395 204
NspI r_CATG'y
Cuts at: 3636 5485 6021 6523 7023 8626 3636
Size: 1849 536 502 500 1603 4480
Fragments arranged by size:
4480 1849 1603 536 502 500
PasI CC'CwG_GG
Cuts at: 2114 3507 4337 4941 5604 5805 2114
Size: 1393 830 604 663 201 5779
Fragments arranged by size:
5779 1393 830 663 604 201
PciI A'CATG_T
Cuts at: 3632 8622 3632
Size: 4990 4480
PflMI CCAn_nnn'nTGG
Cuts at: 2331 2985 4486 4932 2331
Size: 654 1501 446 6869
Fragments arranged by size:
6869 1501 654 446
PfoI T'CCnGG_A
Cuts at: 722 3042 4055 5507 5696 722
Size: 2320 1013 1452 189 4496
Fragments arranged by size:
4496 2320 1452 1013 189
PmeI GTTT'AAAC
Cuts at: 6469 6469
Size: 9470
PmlI CAC'GTG
Cuts at: 5517 5517
Size: 9470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2775 4627 4659 7916 7948 2743
Size: 32 1852 32 3257 32 4265
Fragments arranged by size:
4265 3257 1852 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 267 366 381 1881 3243 3648 4945 5004 5148
Size: 99 15 1500 1362 405 1297 59 144
Cuts at: 5148 6273 267
Size: 1125 3464
Fragments arranged by size:
3464 1500 1362 1297 1125 405 144 99
59 15
PshAI GACnn'nnGTC
Cuts at: 2993 5212 2993
Size: 2219 7251
PspOMI G'GGCC_C
Cuts at: 1717 3503 5454 1717
Size: 1786 1951 5733
Fragments arranged by size:
5733 1951 1786
PspXI vC'TCGA_Gb
Cuts at: 3403 3403
Size: 9470
PstI C_TGCA'G
Cuts at: 470 942 1976 2323 2463 2558 3483 4997 5549
Size: 472 1034 347 140 95 925 1514 552
Cuts at: 5549 5576 6002 6517 7202 470
Size: 27 426 515 685 2738
Fragments arranged by size:
2738 1514 1034 925 685 552 515 472
426 347 140 95 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9470
PvuII CAG'CTG
Cuts at: 897 2193 2295 2388 3484 4206 4749 4932 5863
Size: 1296 102 93 1096 722 543 183 931
Cuts at: 5863 6063 6895 7255 897
Size: 200 832 360 3112
Fragments arranged by size:
3112 1296 1096 931 832 722 543 360
200 183 102 93
RsrII CG'GwC_CG
Cuts at: 7665 7665
Size: 9470
SacI G_AGCT'C
Cuts at: 1787 2309 2456 3989 4349 6484 1787
Size: 522 147 1533 360 2135 4773
Fragments arranged by size:
4773 2135 1533 522 360 147
SalI G'TCGA_C
Cuts at: 5215 5584 6507 5215
Size: 369 923 8178
Fragments arranged by size:
8178 923 369
SanDI GG'GwC_CC
Cuts at: 3243 4945 3243
Size: 1702 7768
SapI GCTCTTCn'nnn_
Cuts at: 7493 7703 7493
Size: 210 9260
SbfI CC_TGCA'GG
Cuts at: 470 6517 470
Size: 6047 3423
ScaI AGT'ACT
Cuts at: 1936 8987 1936
Size: 7051 2419
SfcI C'TryA_G
Cuts at: 33 466 938 1972 2319 2459 2554 2653 3479
Size: 433 472 1034 347 140 95 99 826
Cuts at: 3479 4993 5535 5545 5572 5998 6513 7198 8166
Size: 1514 542 10 27 426 515 685 968
Cuts at: 8166 8357 33
Size: 191 1146
Fragments arranged by size:
1514 1146 1034 968 826 685 542 515
472 433 426 347 191 140 99 95
27 10
SfoI GGC'GCC
Cuts at: 892 2697 3445 6283 6902 7150 892
Size: 1805 748 2838 619 248 3212
Fragments arranged by size:
3212 2838 1805 748 619 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9470
SmaI CCC'GGG
Cuts at: 743 798 3646 6492 743
Size: 55 2848 2846 3721
Fragments arranged by size:
3721 2848 2846 55
SmlI C'TyrA_G
Cuts at: 271 3403 3496 3558 6268 6625 7540 8254 8516
Size: 3132 93 62 2710 357 915 714 262
Cuts at: 8516 271
Size: 1225
Fragments arranged by size:
3132 2710 1225 915 714 357 262 93
62
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9470
SphI G_CATG'C
Cuts at: 6021 6523 7023 6021
Size: 502 500 8468
Fragments arranged by size:
8468 502 500
SspI AAT'ATT
Cuts at: 5407 5407
Size: 9470
StuI AGG'CCT
Cuts at: 4765 4765
Size: 9470
StyI C'CwwG_G
Cuts at: 362 617 760 2903 3107 5000 5186 6053 6188
Size: 255 143 2143 204 1893 186 867 135
Cuts at: 6188 6277 6604 6958 362
Size: 89 327 354 2874
Fragments arranged by size:
2874 2143 1893 867 354 327 255 204
186 143 135 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2323 2377 2410 3276 5304 7053 7186 7645 7854
Size: 54 33 866 2028 1749 133 459 209
Cuts at: 7854 2323
Size: 3939
Fragments arranged by size:
3939 2028 1749 866 459 209 133 54
33
TatI w'GTAC_w
Cuts at: 1934 2069 3260 8985 1934
Size: 135 1191 5725 2419
Fragments arranged by size:
5725 2419 1191 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4599 4631 5367 5399 8987 9019 4599
Size: 32 736 32 3588 32 5050
Fragments arranged by size:
5050 3588 736 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 175 594 2539 7267 175
Size: 419 1945 4728 2378
Fragments arranged by size:
4728 2378 1945 419
XbaI T'CTAG_A
Cuts at: 829 6501 829
Size: 5672 3798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 697 2271 3834 3859 3926 4021 4116 4167 4431
Size: 1574 1563 25 67 95 95 51 264
Cuts at: 4431 6965 697
Size: 2534 3202
Fragments arranged by size:
3202 2534 1574 1563 264 95 95 67
51 25
XhoI C'TCGA_G
Cuts at: 3403 3403
Size: 9470
XmaI C'CCGG_G
Cuts at: 741 796 3644 6490 741
Size: 55 2848 2846 3721
Fragments arranged by size:
3721 2848 2846 55
XmnI GAAnn'nnTTC
Cuts at: 346 1357 2029 2758 6457 346
Size: 1011 672 729 3699 3359
Fragments arranged by size:
3699 3359 1011 729 672
ZraI GAC'GTC
Cuts at: 2880 2880
Size: 9470
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AlfI AloI AscI AvrII BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstZ17I EcoRV FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI