Restriction map for
pF1KF0190
(Circular) (Six-base) MAPSORT of: pF1KF0190.seq Check: 5646 from: 1 to: 4673
pF1KF0190 4673 bp
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 763 763
Size: 4673
AccI GT'mk_AC
Cuts at: 303 1711 303
Size: 1408 3265
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 388 595 1247 1498 2498 2930 3677 3945
Size: 187 207 652 251 1000 432 747 268
Cuts at: 3945 201
Size: 929
Fragments arranged by size:
1000 929 747 652 432 268 251 207
187
AfeI AGC'GCT
Cuts at: 124 124
Size: 4673
AflIII A'CryG_T
Cuts at: 402 713 2025 3129 4106 402
Size: 311 1312 1104 977 969
Fragments arranged by size:
1312 1104 977 969 311
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 381 1274 1521 1978 3545 381
Size: 893 247 457 1567 1509
Fragments arranged by size:
1567 1509 893 457 247
ApaI G_GGCC'C
Cuts at: 119 133 906 1038 119
Size: 14 773 132 3754
Fragments arranged by size:
3754 773 132 14
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1393 1677 4026 4282 1393
Size: 284 2349 256 1784
Fragments arranged by size:
2349 1784 284 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 118 1025 1407 1693 118
Size: 907 382 286 3098
Fragments arranged by size:
3098 907 382 286
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 808 841 808
Size: 33 4640
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1367 1462 1689 2103 2351 2386 1367
Size: 95 227 414 248 35 3654
Fragments arranged by size:
3654 414 248 227 95 35
BanII G_rGCy'C
Cuts at: 119 133 292 906 1038 1224 1427 1687 2717
Size: 14 159 614 132 186 203 260 1030
Cuts at: 2717 119
Size: 2075
Fragments arranged by size:
2075 1030 614 260 203 186 159 132
14
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 862 862
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglI GCCn_nnn'nGGC
Cuts at: 787 787
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 119 133 906 1038 1184 1370 1465 2298 2391
Size: 14 773 132 146 186 95 833 93
Cuts at: 2391 3447 119
Size: 1056 1345
Fragments arranged by size:
1345 1056 833 773 186 146 132 95
93 14
BmrI ACTGGGnnnn_n'
Cuts at: 795 1498 1885 1966 2301 4445 795
Size: 703 387 81 335 2144 1023
Fragments arranged by size:
2144 1023 703 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 649 787 1160 146
Size: 503 138 373 3659
Fragments arranged by size:
3659 503 373 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3220 3518 1849
Size: 879 492 298 3004
Fragments arranged by size:
3004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 539 2026 539
Size: 1487 3186
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 1021 1118 2104 2352 3054 4550 1021
Size: 97 986 248 702 1496 1144
Fragments arranged by size:
1496 1144 986 702 248 97
BsaWI w'CCGG_w
Cuts at: 1473 1862 2383 3061 3335 3482 3893 1473
Size: 389 521 678 274 147 411 2253
Fragments arranged by size:
2253 678 521 411 389 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 159 189 402 432 1429 1459 159
Size: 30 213 30 997 30 3373
Fragments arranged by size:
3373 997 213 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 1102 1105 1114 1214 1236 1284 1434 1564
Size: 573 3 9 100 22 48 150 130
Cuts at: 1564 1573 529
Size: 9 3629
Fragments arranged by size:
3629 573 150 130 100 48 22 9
9 3
BseYI C'CCAG_C
Cuts at: 125 421 949 1038 1274 1309 1532 1601 2098
Size: 296 528 89 236 35 223 69 497
Cuts at: 2098 2856 3433 3929 125
Size: 758 577 496 869
Fragments arranged by size:
869 758 577 528 497 496 296 236
223 89 69 35
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 292 503 1169 1427 1618 1687 2465 2655 3447
Size: 211 666 258 191 69 778 190 792
Cuts at: 3447 292
Size: 1518
Fragments arranged by size:
1518 792 778 666 258 211 191 190
69
BsmI GAATG_Cn'
Cuts at: 589 589
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 119 133 292 503 906 1038 1169 1184 1224
Size: 14 159 211 403 132 131 15 40
Cuts at: 1224 1370 1427 1465 1618 1687 2298 2391 2465
Size: 146 57 38 153 69 611 93 74
Cuts at: 2465 2655 2717 3447 119
Size: 190 62 730 1345
Fragments arranged by size:
1345 730 611 403 211 190 159 153
146 132 131 93 74 69 62 57
40 38 15 14
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 1723 1937 2239 2620 763
Size: 960 214 302 381 2816
Fragments arranged by size:
2816 960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 160 2965 3019 4503 4668 50
Size: 110 2805 54 1484 165 55
Fragments arranged by size:
2805 1484 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 480 2671 2852 3061 480
Size: 2191 181 209 2092
Fragments arranged by size:
2191 2092 209 181
BsrGI T'GTAC_A
Cuts at: 383 383
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 1287 1287
Size: 4673
BstYI r'GATC_y
Cuts at: 1 518 570 1698 2165 2188 2523 2769 3781
Size: 517 52 1128 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1128 1012 517 467 449 346 335 246
86 52 23 12
Bsu36I CC'TnA_GG
Cuts at: 1547 1547
Size: 4673
BtgI C'CryG_G
Cuts at: 107 639 898 107
Size: 532 259 3882
Fragments arranged by size:
3882 532 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 791 1604 2623 2804 2967 791
Size: 813 1019 181 163 2497
Fragments arranged by size:
2497 1019 813 181 163
BtsI GCAGTG_nn'
Cuts at: 687 1916 687
Size: 1229 3444
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DraIII CAC_nnn'GTG
Cuts at: 933 933
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 642 1661 2258 2432 2823 2850 4195 642
Size: 1019 597 174 391 27 1345 1120
Fragments arranged by size:
1345 1120 1019 597 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 317 1079 1415 1517 1550 2696 2906 317
Size: 762 336 102 33 1146 210 2084
Fragments arranged by size:
2084 1146 762 336 210 102 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 290 1425 1685 290
Size: 1135 260 3278
Fragments arranged by size:
3278 1135 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 201 388 595 649 787 1160 1247 1498
Size: 55 187 207 54 138 373 87 251
Cuts at: 1498 2498 2930 3677 3945 146
Size: 1000 432 747 268 874
Fragments arranged by size:
1000 874 747 432 373 268 251 207
187 138 87 55 54
EcoNI CCTnn'n_nnAGG
Cuts at: 151 1075 1302 151
Size: 924 227 3522
Fragments arranged by size:
3522 924 227
EcoO109I rG'GnC_Cy
Cuts at: 115 129 130 170 378 771 903 1034 1035
Size: 14 1 40 208 393 132 131 1
Cuts at: 1035 1068 1313 1498 1812 115
Size: 33 245 185 314 2976
Fragments arranged by size:
2976 393 314 245 208 185 132 131
40 33 14 1 1
EcoRI G'AATT_C
Cuts at: 1393 1677 1393
Size: 284 4389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 126 2107 2355 3377 4356 126
Size: 1981 248 1022 979 443
Fragments arranged by size:
1981 1022 979 443 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 402 434 1426 1458 2515 2545 2547
Size: 32 211 32 992 32 1057 30 2
Cuts at: 2547 2577 159
Size: 30 2255
Fragments arranged by size:
2255 1057 992 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 402 4106 402
Size: 3704 969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3344 3528 3344
Size: 184 4489
MscI TGG'CCA
Cuts at: 644 1663 2434 644
Size: 1019 771 2883
Fragments arranged by size:
2883 1019 771
MslI CAynn'nnrTG
Cuts at: 2662 2789 2662
Size: 127 4546
MspA1I CmG'CkG
Cuts at: 546 673 885 1601 1784 2098 2458 3471 3716
Size: 127 212 716 183 314 360 1013 245
Cuts at: 3716 546
Size: 1503
Fragments arranged by size:
1503 1013 716 360 314 245 212 183
127
NaeI GCC'GGC
Cuts at: 482 2854 482
Size: 2372 2301
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 639 107
Size: 532 4141
NgoMIV G'CCGG_C
Cuts at: 480 2852 480
Size: 2372 2301
NspI r_CATG'y
Cuts at: 561 1726 2226 3133 561
Size: 1165 500 907 2101
Fragments arranged by size:
2101 1165 907 500
PasI CC'CwG_GG
Cuts at: 241 437 1217 1492 241
Size: 196 780 275 3422
Fragments arranged by size:
3422 780 275 196
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 247 247
Size: 4673
PfoI T'CCnGG_A
Cuts at: 1415 1415
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 378 771 1068 1313 1498 378
Size: 393 297 245 185 3553
Fragments arranged by size:
3553 393 297 245 185
PshAI GACnn'nnGTC
Cuts at: 960 960
Size: 4673
PspOMI G'GGCC_C
Cuts at: 115 129 902 1034 115
Size: 14 773 132 3754
Fragments arranged by size:
3754 773 132 14
PspXI vC'TCGA_Gb
Cuts at: 1025 1025
Size: 4673
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 376 408 376
Size: 32 4641
PstI C_TGCA'G
Cuts at: 155 1720 2405 155
Size: 1565 685 2423
Fragments arranged by size:
2423 1565 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 546 673 1601 2098 2458 546
Size: 127 928 497 360 2761
Fragments arranged by size:
2761 928 497 360 127
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 292 1427 1687 292
Size: 1135 260 3278
Fragments arranged by size:
3278 1135 260
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SanDI GG'GwC_CC
Cuts at: 1498 1498
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 1415 2696 2906 1415
Size: 1281 210 3182
Fragments arranged by size:
3182 1281 210
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 151 1716 2401 3394 3585 33
Size: 118 1565 685 993 191 1121
Fragments arranged by size:
1565 1121 993 685 191 118
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1025 1828 2743 3235 3497 1025
Size: 803 915 492 262 2201
Fragments arranged by size:
2201 915 803 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 561 1726 2226 561
Size: 1165 500 3008
Fragments arranged by size:
3008 1165 500
StyI C'CwwG_G
Cuts at: 107 338 639 1347 1400 1807 2161 107
Size: 231 301 708 53 407 354 2619
Fragments arranged by size:
2619 708 407 354 301 231 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 429 520 1060 1633 1642 2256 2389 2848 3057
Size: 91 540 573 9 614 133 459 209
Cuts at: 3057 429
Size: 2045
Fragments arranged by size:
2045 614 573 540 459 209 133 91
9
TatI w'GTAC_w
Cuts at: 383 4188 383
Size: 3805 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1136 1168 4190 4222 1136
Size: 32 3022 32 1587
Fragments arranged by size:
3022 1587 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 782 1224 1354 2168 782
Size: 442 130 814 3287
Fragments arranged by size:
3287 814 442 130
XhoI C'TCGA_G
Cuts at: 1025 1025
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI