Restriction map for
pF1KF0229
(Circular) (Six-base) MAPSORT of: pF1KF0229.seq Check: 3084 from: 1 to: 4937
pF1KF0229 4937 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 788 858 788
Size: 70 4867
AccI GT'mk_AC
Cuts at: 1772 1975 1772
Size: 203 4734
Acc65I G'GTAC_C
Cuts at: 1953 1953
Size: 4937
AclI AA'CG_TT
Cuts at: 4777 4777
Size: 4937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1056 1294 2762 3194 3547 4209 1056
Size: 238 1468 432 353 662 1784
Fragments arranged by size:
1784 1468 662 432 353 238
AflIII A'CryG_T
Cuts at: 820 2289 4089 4370 820
Size: 1469 1800 281 1387
Fragments arranged by size:
1800 1469 1387 281
AlwNI CAG_nnn'CTG
Cuts at: 1030 2242 3680 1030
Size: 1212 1438 2287
Fragments arranged by size:
2287 1438 1212
ApaI G_GGCC'C
Cuts at: 124 140 124
Size: 16 4921
ApaLI G'TGCA_C
Cuts at: 3775 3775
Size: 4937
ApoI r'AATT_y
Cuts at: 420 1111 1244 1549 1644 1941 4290 4546 420
Size: 691 133 305 95 297 2349 256 811
Fragments arranged by size:
2349 811 691 305 297 256 133 95
AscI GG'CGCG_CC
Cuts at: 218 218
Size: 4937
AseI AT'TA_AT
Cuts at: 20 20
Size: 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
AvaI C'yCGr_G
Cuts at: 1957 1957
Size: 4937
BamHI G'GATC_C
Cuts at: 543 1742 1962 543
Size: 1199 220 3518
Fragments arranged by size:
3518 1199 220
BanI G'GyrC_C
Cuts at: 192 1002 1953 2367 2615 2650 192
Size: 810 951 414 248 35 2479
Fragments arranged by size:
2479 951 810 414 248 35
BanII G_rGCy'C
Cuts at: 124 140 989 1951 2981 124
Size: 16 849 962 1030 2080
Fragments arranged by size:
2080 1030 962 849 16
BbeI G_GCGC'C
Cuts at: 196 1006 2371 2619 196
Size: 810 1365 248 2514
Fragments arranged by size:
2514 1365 810 248
BbsI GAAGACnn'nnnn_
Cuts at: 461 461
Size: 4937
BbvCI CC'TCA_GC
Cuts at: 356 1580 356
Size: 1224 3713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1795 1829 1795
Size: 34 4903
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3891 4937 2835
Size: 1056 1046 2835
Fragments arranged by size:
2835 1056 1046
BclI T'GATC_A
Cuts at: 632 2457 632
Size: 1825 3112
BglI GCCn_nnn'nGGC
Cuts at: 128 205 128
Size: 77 4860
BglII A'GATC_T
Cuts at: 2452 4489 2452
Size: 2037 2900
BlpI GC'TnA_GC
Cuts at: 2049 2049
Size: 4937
Bme1580I G_kGCm'C
Cuts at: 124 140 2562 2655 3779 124
Size: 16 2422 93 1124 1282
Fragments arranged by size:
2422 1282 1124 93 16
BmrI ACTGGGnnnn_n'
Cuts at: 438 751 931 2149 2230 2565 4709 438
Size: 313 180 1218 81 335 2144 666
Fragments arranged by size:
2144 1218 666 335 313 180 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1538 3304 1538
Size: 1766 3171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1177 1325 343
Size: 834 148 3955
Fragments arranged by size:
3955 834 148
Bpu10I CC'TnA_GC
Cuts at: 356 1250 1580 356
Size: 894 330 3713
Fragments arranged by size:
3713 894 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2113 2992 3706 4004 1585
Size: 528 879 714 298 2518
Fragments arranged by size:
2518 879 714 528 298
BsaI GGTCTCn'nnnn_
Cuts at: 1217 4641 1217
Size: 3424 1513
BsaAI yAC'GTr
Cuts at: 2290 2290
Size: 4937
BsaBI GATnn'nnATC
Cuts at: 6 740 2451 4488 6
Size: 734 1711 2037 455
Fragments arranged by size:
2037 1711 734 455
BsaHI Gr'CG_yC
Cuts at: 193 1003 2368 2616 3318 4814 193
Size: 810 1365 248 702 1496 316
Fragments arranged by size:
1496 1365 810 702 316 248
BsaWI w'CCGG_w
Cuts at: 2126 2647 3325 3736 3883 4157 2126
Size: 521 678 411 147 274 2906
Fragments arranged by size:
2906 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 1125 1155 265
Size: 30 830 30 4047
Fragments arranged by size:
4047 830 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 492 492
Size: 4937
BseYI C'CCAG_C
Cuts at: 269 327 2362 3120 3785 4193 269
Size: 58 2035 758 665 408 1013
Fragments arranged by size:
2035 1013 758 665 408 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 874 277
Size: 597 4340
BsiEI CG_ry'CG
Cuts at: 104 2479 2525 3755 104
Size: 2375 46 1230 1286
Fragments arranged by size:
2375 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 674 1823 1951 2729 2919 3779 674
Size: 1149 128 778 190 860 1832
Fragments arranged by size:
1832 1149 860 778 190 128
BsmI GAATG_Cn'
Cuts at: 360 360
Size: 4937
Bsp1286I G_dGCh'C
Cuts at: 124 140 674 989 1823 1951 2562 2655 2729
Size: 16 534 315 834 128 611 93 74
Cuts at: 2729 2919 2981 3779 124
Size: 190 62 798 1282
Fragments arranged by size:
1282 834 798 611 534 315 190 128
93 74 62 16
BspHI T'CATG_A
Cuts at: 825 906 1101 3369 825
Size: 81 195 2268 2393
Fragments arranged by size:
2393 2268 195 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 788 858 1245 1987 2201 2503 2884 788
Size: 70 387 742 214 302 381 2841
Fragments arranged by size:
2841 742 387 381 302 214 70
BsrBI CCG'CTC
Cuts at: 50 3229 3283 4767 4932 50
Size: 3179 54 1484 165 55
Fragments arranged by size:
3179 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1885 2849 1885
Size: 964 3973
BsrFI r'CCGG_y
Cuts at: 195 1622 2935 3116 195
Size: 1427 1313 181 2016
Fragments arranged by size:
2016 1427 1313 181
BsrGI T'GTAC_A
Cuts at: 1374 1374
Size: 4937
BssHII G'CGCG_C
Cuts at: 218 218
Size: 4937
BssSI C'ACGA_G
Cuts at: 3208 3916 3208
Size: 708 4229
BstAPI GCAn_nnn'nTGC
Cuts at: 2314 2314
Size: 4937
BstBI TT'CG_AA
Cuts at: 1215 3298 1215
Size: 2083 2854
BstYI r'GATC_y
Cuts at: 1 543 1742 1962 2429 2452 2787 3033 3339
Size: 542 1199 220 467 23 335 246 306
Cuts at: 3339 3351 3437 4489 1
Size: 12 86 1052 449
Fragments arranged by size:
1199 1052 542 467 449 335 306 246
220 86 23 12
BtgI C'CryG_G
Cuts at: 107 249 338 1507 107
Size: 142 89 1169 3537
Fragments arranged by size:
3537 1169 142 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2887 3068 3231 2887
Size: 181 163 4593
Fragments arranged by size:
4593 181 163
BtsI GCAGTG_nn'
Cuts at: 294 2180 294
Size: 1886 3051
ClaI AT'CG_AT
Cuts at: 744 744
Size: 4937
DraI TTT'AAA
Cuts at: 1334 1936 1334
Size: 602 4335
DraIII CAC_nnn'GTG
Cuts at: 300 669 300
Size: 369 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2643 3987 2643
Size: 1344 3593
EaeI y'GGCC_r
Cuts at: 246 252 1201 2522 2696 3087 3114 4459 246
Size: 6 949 1321 174 391 27 1345 724
Fragments arranged by size:
1345 1321 949 724 391 174 27 6
EagI C'GGCC_G
Cuts at: 2522 2522
Size: 4937
EarI CTCTTCn'nnn_
Cuts at: 230 1340 1507 1718 2960 3170 230
Size: 1110 167 211 1242 210 1997
Fragments arranged by size:
1997 1242 1110 211 210 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 273 1133 3145 3887 4033 4747 273
Size: 860 2012 742 146 714 463
Fragments arranged by size:
2012 860 742 714 463 146
EcoICRI GAG'CTC
Cuts at: 1949 1949
Size: 4937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1056 1177 1294 1325 2762 3194 3547 4209
Size: 713 121 117 31 1437 432 353 662
Cuts at: 4209 343
Size: 1071
Fragments arranged by size:
1437 1071 713 662 432 353 121 117
31
EcoO109I rG'GnC_Cy
Cuts at: 120 121 136 258 1576 2076 120
Size: 1 15 122 1318 500 2981
Fragments arranged by size:
2981 1318 500 122 15 1
EcoRI G'AATT_C
Cuts at: 1111 1941 1111
Size: 830 4107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4937
FspI TGC'GCA
Cuts at: 2718 2718
Size: 4937
HaeII r_GCGC'y
Cuts at: 196 1006 2371 2619 3849 4620 196
Size: 810 1365 248 1230 771 513
Fragments arranged by size:
1365 1230 810 771 513 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 2779 2809 2811 2841 265
Size: 32 2482 30 2 30 2361
Fragments arranged by size:
2482 2361 32 30 30 2
HincII GTy'rAC
Cuts at: 860 1047 1976 860
Size: 187 929 3821
Fragments arranged by size:
3821 929 187
HindIII A'AGCT_T
Cuts at: 1476 1476
Size: 4937
HpaI GTT'AAC
Cuts at: 860 1047 860
Size: 187 4750
KasI G'GCGC_C
Cuts at: 192 1002 2367 2615 192
Size: 810 1365 248 2514
Fragments arranged by size:
2514 1365 810 248
KpnI G_GTAC'C
Cuts at: 1957 1957
Size: 4937
MluI A'CGCG_T
Cuts at: 4370 4370
Size: 4937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 600 1107 3696 3880 137
Size: 463 507 2589 184 1194
Fragments arranged by size:
2589 1194 507 463 184
MscI TGG'CCA
Cuts at: 1203 2698 1203
Size: 1495 3442
MslI CAynn'nnrTG
Cuts at: 793 1106 1868 2926 3053 793
Size: 313 762 1058 127 2677
Fragments arranged by size:
2677 1058 762 313 127
MspA1I CmG'CkG
Cuts at: 2048 2362 2722 3506 3751 2048
Size: 314 360 784 245 3234
Fragments arranged by size:
3234 784 360 314 245
NaeI GCC'GGC
Cuts at: 197 3118 197
Size: 2921 2016
NarI GG'CG_CC
Cuts at: 193 1003 2368 2616 193
Size: 810 1365 248 2514
Fragments arranged by size:
2514 1365 810 248
NcoI C'CATG_G
Cuts at: 107 1507 107
Size: 1400 3537
NgoMIV G'CCGG_C
Cuts at: 195 3116 195
Size: 2921 2016
NspI r_CATG'y
Cuts at: 824 1990 2490 4093 824
Size: 1166 500 1603 1668
Fragments arranged by size:
1668 1603 1166 500
PciI A'CATG_T
Cuts at: 820 4089 820
Size: 3269 1668
PflMI CCAn_nnn'nTGG
Cuts at: 339 1692 339
Size: 1353 3584
PfoI T'CCnGG_A
Cuts at: 500 500
Size: 4937
PmeI GTTT'AAAC
Cuts at: 1936 1936
Size: 4937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4905
PpuMI rG'GwC_Cy
Cuts at: 1576 1576
Size: 4937
PspOMI G'GGCC_C
Cuts at: 120 136 120
Size: 16 4921
PstI C_TGCA'G
Cuts at: 1984 2669 1984
Size: 685 4252
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4937
PvuII CAG'CTG
Cuts at: 2362 2722 2362
Size: 360 4577
RsrII CG'GwC_CG
Cuts at: 3132 3132
Size: 4937
SacI G_AGCT'C
Cuts at: 1951 1951
Size: 4937
SalI G'TCGA_C
Cuts at: 1974 1974
Size: 4937
SapI GCTCTTCn'nnn_
Cuts at: 2960 3170 2960
Size: 210 4727
SbfI CC_TGCA'GG
Cuts at: 1984 1984
Size: 4937
ScaI AGT'ACT
Cuts at: 1069 4454 1069
Size: 3385 1552
SfcI C'TryA_G
Cuts at: 33 1235 1980 2665 3633 3824 33
Size: 1202 745 685 968 191 1146
Fragments arranged by size:
1202 1146 968 745 685 191
SfoI GGC'GCC
Cuts at: 194 1004 2369 2617 194
Size: 810 1365 248 2514
Fragments arranged by size:
2514 1365 810 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
SmaI CCC'GGG
Cuts at: 1959 1959
Size: 4937
SmlI C'TyrA_G
Cuts at: 1564 2092 3007 3721 3983 1564
Size: 528 915 714 262 2518
Fragments arranged by size:
2518 915 714 528 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4937
SphI G_CATG'C
Cuts at: 1990 2490 1990
Size: 500 4437
SspI AAT'ATT
Cuts at: 393 393
Size: 4937
StyI C'CwwG_G
Cuts at: 107 623 1507 2071 2425 107
Size: 516 884 564 354 2619
Fragments arranged by size:
2619 884 564 516 354
SwaI ATTT'AAAT
Cuts at: 1334 1334
Size: 4937
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2520 2653 3112 3321 2520
Size: 133 459 209 4136
Fragments arranged by size:
4136 459 209 133
TatI w'GTAC_w
Cuts at: 1067 1374 4452 1067
Size: 307 3078 1552
Fragments arranged by size:
3078 1552 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4454 4486 4454
Size: 32 4905
Tth111I GACn'n_nGTC
Cuts at: 1777 2734 1777
Size: 957 3980
XbaI T'CTAG_A
Cuts at: 1591 1707 1968 1591
Size: 116 261 4560
Fragments arranged by size:
4560 261 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2432 2432
Size: 4937
XmaI C'CCGG_G
Cuts at: 1957 1957
Size: 4937
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BstEII BstXI BstZ17I Bsu36I CspCI EcoNI EcoRV FseI
FspAI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI XhoI XmnI
ZraI