Restriction map for
pF1KF0267
(Circular) (Six-base) MAPSORT of: pF1KF0267.seq Check: 9060 from: 1 to: 6416
pF1KF0267 6416 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 191 872 2997 191
Size: 681 2125 3610
Fragments arranged by size:
3610 2125 681
AccI GT'mk_AC
Cuts at: 2332 3454 2332
Size: 1122 5294
Acc65I G'GTAC_C
Cuts at: 2525 3432 2525
Size: 907 5509
AclI AA'CG_TT
Cuts at: 1418 6256 1418
Size: 4838 1578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2609 2714 3290 4241 4673 5026 5688 1922
Size: 687 105 576 951 432 353 662 2650
Fragments arranged by size:
2650 951 687 662 576 432 353 105
AfeI AGC'GCT
Cuts at: 484 484
Size: 6416
AflIII A'CryG_T
Cuts at: 735 3768 5568 5849 735
Size: 3033 1800 281 1302
Fragments arranged by size:
3033 1800 1302 281
AhdI GACnn_n'nnGTC
Cuts at: 1611 1611
Size: 6416
AleI CACnn'nnGTG
Cuts at: 736 736
Size: 6416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3015 3015
Size: 6416
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 506 538 506
Size: 32 6384
AlwNI CAG_nnn'CTG
Cuts at: 1669 1871 2040 2587 3721 5159 1669
Size: 202 169 547 1134 1438 2926
Fragments arranged by size:
2926 1438 1134 547 202 169
ApaI G_GGCC'C
Cuts at: 1148 2427 1148
Size: 1279 5137
ApaLI G'TGCA_C
Cuts at: 5254 5254
Size: 6416
ApoI r'AATT_y
Cuts at: 232 313 3420 5769 6025 232
Size: 81 3107 2349 256 623
Fragments arranged by size:
3107 2349 623 256 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 6416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
AvaI C'yCGr_G
Cuts at: 812 829 3003 3053 3119 3436 812
Size: 17 2174 50 66 317 3792
Fragments arranged by size:
3792 2174 317 66 50 17
BamHI G'GATC_C
Cuts at: 414 3289 3441 414
Size: 2875 152 3389
Fragments arranged by size:
3389 2875 152
BanI G'GyrC_C
Cuts at: 204 2525 2925 2985 3432 3846 4094 4129 204
Size: 2321 400 60 447 414 248 35 2491
Fragments arranged by size:
2491 2321 447 414 400 248 60 35
BanII G_rGCy'C
Cuts at: 630 1148 1775 2427 3263 3430 4460 630
Size: 518 627 652 836 167 1030 2586
Fragments arranged by size:
2586 1030 836 652 627 518 167
BbeI G_GCGC'C
Cuts at: 3850 4098 3850
Size: 248 6168
BbsI GAAGACnn'nnnn_
Cuts at: 2567 2567
Size: 6416
BbvCI CC'TCA_GC
Cuts at: 452 640 1037 2444 2480 2642 2786 452
Size: 188 397 1407 36 162 144 4082
Fragments arranged by size:
4082 1407 397 188 162 144 36
BciVI GTATCCnnnnn_n'
Cuts at: 4314 5370 6416 4314
Size: 1056 1046 4314
Fragments arranged by size:
4314 1056 1046
BclI T'GATC_A
Cuts at: 3936 3936
Size: 6416
BfrBI ATG'CAT
Cuts at: 600 3370 600
Size: 2770 3646
BglI GCCn_nnn'nGGC
Cuts at: 1134 1297 2811 2861 3015 1134
Size: 163 1514 50 154 4535
Fragments arranged by size:
4535 1514 163 154 50
BglII A'GATC_T
Cuts at: 3931 5968 3931
Size: 2037 4379
BlpI GC'TnA_GC
Cuts at: 2096 3528 2096
Size: 1432 4984
Bme1580I G_kGCm'C
Cuts at: 209 588 1148 1400 1456 2427 2930 4041 4134
Size: 379 560 252 56 971 503 1111 93
Cuts at: 4134 5258 209
Size: 1124 1367
Fragments arranged by size:
1367 1124 1111 971 560 503 379 252
93 56
BmrI ACTGGGnnnn_n'
Cuts at: 1252 2813 2951 3628 3709 4044 6188 1252
Size: 1561 138 677 81 335 2144 1480
Fragments arranged by size:
2144 1561 1480 677 335 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1821 4783 1821
Size: 2962 3454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1621 1786 2026 2123 2681 2737 3053 3277
Size: 1059 165 240 97 558 56 316 224
Cuts at: 3277 562
Size: 3701
Fragments arranged by size:
3701 1059 558 316 240 224 165 97
56
Bpu10I CC'TnA_GC
Cuts at: 452 479 640 1037 2444 2480 2642 2786 3247
Size: 27 161 397 1407 36 162 144 461
Cuts at: 3247 452
Size: 3621
Fragments arranged by size:
3621 1407 461 397 162 161 144 36
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 1539 1638 3128 3592 4471 5185 5483 1468
Size: 71 99 1490 464 879 714 298 2401
Fragments arranged by size:
2401 1490 879 714 464 298 99 71
BsaI GGTCTCn'nnnn_
Cuts at: 288 2140 2642 6120 288
Size: 1852 502 3478 584
Fragments arranged by size:
3478 1852 584 502
BsaAI yAC'GTr
Cuts at: 738 3769 738
Size: 3031 3385
BsaBI GATnn'nnATC
Cuts at: 6 3930 5967 6
Size: 3924 2037 455
Fragments arranged by size:
3924 2037 455
BsaHI Gr'CG_yC
Cuts at: 3847 4095 4797 6293 3847
Size: 248 702 1496 3970
Fragments arranged by size:
3970 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3605 4126 4804 5215 5362 5636 3605
Size: 521 678 411 147 274 4385
Fragments arranged by size:
4385 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 775 805 1621 1651 2509 2539 361
Size: 30 384 30 816 30 858 30 4238
Fragments arranged by size:
4238 858 816 384 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 276 369 1783 1813 1924 2336 2461 2609
Size: 20 93 1414 30 111 412 125 148
Cuts at: 2609 2809 3227 256
Size: 200 418 3445
Fragments arranged by size:
3445 1414 418 412 200 148 125 111
93 30 20
BseYI C'CCAG_C
Cuts at: 1111 1147 1279 1300 1864 2069 2191 3841 4599
Size: 36 132 21 564 205 122 1650 758
Cuts at: 4599 5264 5672 1111
Size: 665 408 1855
Fragments arranged by size:
1855 1650 758 665 564 408 205 132
122 36 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 1337 1645 3296 248
Size: 1089 308 1651 3368
Fragments arranged by size:
3368 1651 1089 308
BsiEI CG_ry'CG
Cuts at: 104 3958 4004 5234 104
Size: 3854 46 1230 1286
Fragments arranged by size:
3854 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 182 941 1775 3263 3430 4208 4398 5258 182
Size: 759 834 1488 167 778 190 860 1340
Fragments arranged by size:
1488 1340 860 834 778 759 190 167
BsmBI CGTCTCn'nnnn_
Cuts at: 381 381
Size: 6416
Bsp1286I G_dGCh'C
Cuts at: 182 209 588 630 941 1148 1400 1456 1775
Size: 27 379 42 311 207 252 56 319
Cuts at: 1775 2427 2930 3263 3430 4041 4134 4208 4398
Size: 652 503 333 167 611 93 74 190
Cuts at: 4398 4460 5258 182
Size: 62 798 1340
Fragments arranged by size:
1340 798 652 611 503 379 333 319
311 252 207 190 167 93 74 62
56 42 27
BspHI T'CATG_A
Cuts at: 551 4848 551
Size: 4297 2119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 347 644 872 2997 3350 3466 3680 3982
Size: 156 297 228 2125 353 116 214 302
Cuts at: 3982 4363 191
Size: 381 2244
Fragments arranged by size:
2244 2125 381 353 302 297 228 214
156 116
BsrBI CCG'CTC
Cuts at: 50 153 327 2912 4708 4762 6246 6411 50
Size: 103 174 2585 1796 54 1484 165 55
Fragments arranged by size:
2585 1796 1484 174 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 1168 2969 4328 1168
Size: 1801 1359 3256
Fragments arranged by size:
3256 1801 1359
BsrFI r'CCGG_y
Cuts at: 431 1469 3014 4414 4595 431
Size: 1038 1545 1400 181 2252
Fragments arranged by size:
2252 1545 1400 1038 181
BsrGI T'GTAC_A
Cuts at: 2463 2463
Size: 6416
BssSI C'ACGA_G
Cuts at: 1274 4687 5395 1274
Size: 3413 708 2295
Fragments arranged by size:
3413 2295 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3793 3793
Size: 6416
BstBI TT'CG_AA
Cuts at: 4777 4777
Size: 6416
BstXI CCAn_nnnn'nTGG
Cuts at: 717 751 1089 2554 2659 717
Size: 34 338 1465 105 4474
Fragments arranged by size:
4474 1465 338 105 34
BstYI r'GATC_y
Cuts at: 1 263 414 494 3289 3441 3908 3931 4266
Size: 262 151 80 2795 152 467 23 335
Cuts at: 4266 4512 4818 4830 4916 5968 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2795 1052 467 449 335 306 262 246
152 151 86 80 23 12
BstZ17I GTA'TAC
Cuts at: 2333 2333
Size: 6416
Bsu36I CC'TnA_GG
Cuts at: 663 1229 1790 2807 663
Size: 566 561 1017 4272
Fragments arranged by size:
4272 1017 566 561
BtgI C'CryG_G
Cuts at: 1027 1440 1877 1027
Size: 413 437 5566
Fragments arranged by size:
5566 437 413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 902 4366 4547 4710 902
Size: 3464 181 163 2608
Fragments arranged by size:
3464 2608 181 163
BtsI GCAGTG_nn'
Cuts at: 369 850 1069 3659 369
Size: 481 219 2590 3126
Fragments arranged by size:
3126 2590 481 219
DraI TTT'AAA
Cuts at: 3415 3415
Size: 6416
DraIII CAC_nnn'GTG
Cuts at: 1102 1246 1102
Size: 144 6272
DrdI GACnn_nn'nnGTC
Cuts at: 4122 5466 4122
Size: 1344 5072
EaeI y'GGCC_r
Cuts at: 614 1002 1075 1159 1427 1471 3012 4001 4175
Size: 388 73 84 268 44 1541 989 174
Cuts at: 4175 4566 4593 5938 614
Size: 391 27 1345 1092
Fragments arranged by size:
1541 1345 1092 989 391 388 268 174
84 73 44 27
EagI C'GGCC_G
Cuts at: 4001 4001
Size: 6416
EarI CTCTTCn'nnn_
Cuts at: 2051 2120 2234 2252 2360 2672 2757 2759 4439
Size: 69 114 18 108 312 85 2 1680
Cuts at: 4439 4649 2051
Size: 210 3818
Fragments arranged by size:
3818 1680 312 210 114 108 85 69
18 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4624 5366 5512 6226 4624
Size: 742 146 714 4814
Fragments arranged by size:
4814 742 714 146
EcoICRI GAG'CTC
Cuts at: 1773 3261 3428 1773
Size: 1488 167 4761
Fragments arranged by size:
4761 1488 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1621 1786 1922 2026 2123 2609 2681 2714
Size: 1059 165 136 104 97 486 72 33
Cuts at: 2714 2737 3053 3277 3290 4241 4673 5026 5688
Size: 23 316 224 13 951 432 353 662
Cuts at: 5688 562
Size: 1290
Fragments arranged by size:
1290 1059 951 662 486 432 353 316
224 165 136 104 97 72 33 23
13
EcoNI CCTnn'n_nnAGG
Cuts at: 388 1428 3072 3395 388
Size: 1040 1644 323 3409
Fragments arranged by size:
3409 1644 1040 323
EcoO109I rG'GnC_Cy
Cuts at: 536 809 834 1144 1969 2409 2423 3050 3555
Size: 273 25 310 825 440 14 627 505
Cuts at: 3555 536
Size: 3397
Fragments arranged by size:
3397 825 627 505 440 310 273 25
14
EcoRI G'AATT_C
Cuts at: 3420 3420
Size: 6416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4435 4435
Size: 6416
FspI TGC'GCA
Cuts at: 4197 4197
Size: 6416
HaeII r_GCGC'y
Cuts at: 486 3850 4098 5328 6099 486
Size: 3364 248 1230 771 803
Fragments arranged by size:
3364 1230 803 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1039 1071 1597 1621 1629 1653 1816 1848 2674
Size: 32 526 24 8 24 163 32 826
Cuts at: 2674 2706 3126 3158 3250 3282 4258 4288 4290
Size: 32 420 32 92 32 976 30 2
Cuts at: 4290 4320 1039
Size: 30 3135
Fragments arranged by size:
3135 976 826 526 420 163 92 32
32 32 32 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 1362 2724 3455 1362
Size: 1362 731 4323
Fragments arranged by size:
4323 1362 731
HindIII A'AGCT_T
Cuts at: 301 301
Size: 6416
KasI G'GCGC_C
Cuts at: 3846 4094 3846
Size: 248 6168
KpnI G_GTAC'C
Cuts at: 2529 3436 2529
Size: 907 5509
MfeI C'AATT_G
Cuts at: 469 469
Size: 6416
MluI A'CGCG_T
Cuts at: 5849 5849
Size: 6416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 1082 1517 2925 5175 5359 314
Size: 768 435 1408 2250 184 1371
Fragments arranged by size:
2250 1408 1371 768 435 184
MscI TGG'CCA
Cuts at: 616 1004 1429 4177 616
Size: 388 425 2748 2855
Fragments arranged by size:
2855 2748 425 388
MslI CAynn'nnrTG
Cuts at: 694 715 736 4405 4532 694
Size: 21 21 3669 127 2578
Fragments arranged by size:
3669 2578 127 21 21
MspA1I CmG'CkG
Cuts at: 682 1111 1868 1888 3527 3841 4201 4985 5230
Size: 429 757 20 1639 314 360 784 245
Cuts at: 5230 682
Size: 1868
Fragments arranged by size:
1868 1639 784 757 429 360 314 245
20
NaeI GCC'GGC
Cuts at: 3016 4597 3016
Size: 1581 4835
NarI GG'CG_CC
Cuts at: 3847 4095 3847
Size: 248 6168
NdeI CA'TA_TG
Cuts at: 3366 3366
Size: 6416
NgoMIV G'CCGG_C
Cuts at: 3014 4595 3014
Size: 1581 4835
NsiI A_TGCA'T
Cuts at: 602 3372 602
Size: 2770 3646
NspI r_CATG'y
Cuts at: 600 604 2069 2874 3469 3969 5572 600
Size: 4 1465 805 595 500 1603 1444
Fragments arranged by size:
1603 1465 1444 805 595 500 4
PasI CC'CwG_GG
Cuts at: 1735 1750 3391 1735
Size: 15 1641 4760
Fragments arranged by size:
4760 1641 15
PciI A'CATG_T
Cuts at: 5568 5568
Size: 6416
PflMI CCAn_nnn'nTGG
Cuts at: 623 1279 623
Size: 656 5760
PfoI T'CCnGG_A
Cuts at: 3030 3292 3030
Size: 262 6154
PmeI GTTT'AAAC
Cuts at: 3415 3415
Size: 6416
PmlI CAC'GTG
Cuts at: 738 738
Size: 6416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1396 1428 4862 4894 1396
Size: 32 3434 32 2918
Fragments arranged by size:
3434 2918 32 32
PpuMI rG'GwC_Cy
Cuts at: 536 809 834 1969 2409 3050 536
Size: 273 25 1135 440 641 3902
Fragments arranged by size:
3902 1135 641 440 273 25
PshAI GACnn'nnGTC
Cuts at: 341 1955 2212 2831 341
Size: 1614 257 619 3926
Fragments arranged by size:
3926 1614 619 257
PspOMI G'GGCC_C
Cuts at: 1144 2423 1144
Size: 1279 5137
PstI C_TGCA'G
Cuts at: 188 881 1110 3463 4148 188
Size: 693 229 2353 685 2456
Fragments arranged by size:
2456 2353 693 685 229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6416
PvuII CAG'CTG
Cuts at: 1111 1868 3841 4201 1111
Size: 757 1973 360 3326
Fragments arranged by size:
3326 1973 757 360
RsrII CG'GwC_CG
Cuts at: 4611 4611
Size: 6416
SacI G_AGCT'C
Cuts at: 1775 3263 3430 1775
Size: 1488 167 4761
Fragments arranged by size:
4761 1488 167
SalI G'TCGA_C
Cuts at: 3453 3453
Size: 6416
SanDI GG'GwC_CC
Cuts at: 809 3050 809
Size: 2241 4175
SapI GCTCTTCn'nnn_
Cuts at: 2051 4439 4649 2051
Size: 2388 210 3818
Fragments arranged by size:
3818 2388 210
SbfI CC_TGCA'GG
Cuts at: 3463 3463
Size: 6416
ScaI AGT'ACT
Cuts at: 5933 5933
Size: 6416
SfcI C'TryA_G
Cuts at: 33 184 458 531 877 1106 1199 1462 3459
Size: 151 274 73 346 229 93 263 1997
Cuts at: 3459 4144 5112 5303 33
Size: 685 968 191 1146
Fragments arranged by size:
1997 1146 968 685 346 274 263 229
191 151 93 73
SfoI GGC'GCC
Cuts at: 3848 4096 3848
Size: 248 6168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
SmaI CCC'GGG
Cuts at: 814 3121 3438 814
Size: 2307 317 3792
Fragments arranged by size:
3792 2307 317
SmlI C'TyrA_G
Cuts at: 1447 1554 1653 3143 3571 4486 5200 5462 1447
Size: 107 99 1490 428 915 714 262 2401
Fragments arranged by size:
2401 1490 915 714 428 262 107 99
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6416
SphI G_CATG'C
Cuts at: 604 2069 2874 3469 3969 604
Size: 1465 805 595 500 3051
Fragments arranged by size:
3051 1465 805 595 500
StuI AGG'CCT
Cuts at: 394 661 1899 3009 394
Size: 267 1238 1110 3801
Fragments arranged by size:
3801 1238 1110 267
StyI C'CwwG_G
Cuts at: 1424 1509 2150 2252 2547 3550 3904 1424
Size: 85 641 102 295 1003 354 3936
Fragments arranged by size:
3936 1003 641 354 295 102 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 152 779 1234 1665 2848 3999 4132 4591 4800
Size: 627 455 431 1183 1151 133 459 209
Cuts at: 4800 152
Size: 1768
Fragments arranged by size:
1768 1183 1151 627 459 455 431 209
133
TatI w'GTAC_w
Cuts at: 2463 3169 5931 2463
Size: 706 2762 2948
Fragments arranged by size:
2948 2762 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 361 393 775 807 5933 5965 361
Size: 32 382 32 5126 32 812
Fragments arranged by size:
5126 812 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4213 4213
Size: 6416
XbaI T'CTAG_A
Cuts at: 3447 3447
Size: 6416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 3911 2398
Size: 1513 4903
XmaI C'CCGG_G
Cuts at: 812 3119 3436 812
Size: 2307 317 3792
Fragments arranged by size:
3792 2307 317
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AscI AvrII BaeI BcgI BmgBI
BmtI BsiWI BsmI BspEI BssHII BstEII ClaI CspCI
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
PacI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI