Restriction map for
pF1KF0293
(Circular) (Six-base) MAPSORT of: pF1KF0293.seq Check: 9237 from: 1 to: 6167
pF1KF0293 6167 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 403 2102 2476 2477 403
Size: 1699 374 1 4093
Fragments arranged by size:
4093 1699 374 1
AatII G_ACGT'C
Cuts at: 1679 1679
Size: 6167
AccI GT'mk_AC
Cuts at: 788 3205 788
Size: 2417 3750
Acc65I G'GTAC_C
Cuts at: 302 3183 302
Size: 2881 3286
AclI AA'CG_TT
Cuts at: 6007 6007
Size: 6167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 882 926 1411 2510 3992 4424 5171 5439 882
Size: 44 485 1099 1482 432 747 268 1610
Fragments arranged by size:
1610 1482 1099 747 485 432 268 44
AfeI AGC'GCT
Cuts at: 2124 2124
Size: 6167
AflIII A'CryG_T
Cuts at: 3519 4623 5600 3519
Size: 1104 977 4086
Fragments arranged by size:
4086 1104 977
AgeI A'CCGG_T
Cuts at: 380 4555 380
Size: 4175 1992
AhdI GACnn_n'nnGTC
Cuts at: 2625 2625
Size: 6167
AleI CACnn'nnGTG
Cuts at: 535 695 1465 1810 535
Size: 160 770 345 4892
Fragments arranged by size:
4892 770 345 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2499 2499
Size: 6167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1867 1899 1867
Size: 32 6135
AlwNI CAG_nnn'CTG
Cuts at: 270 1194 1566 3472 5039 270
Size: 924 372 1906 1567 1398
Fragments arranged by size:
1906 1567 1398 924 372
ApaI G_GGCC'C
Cuts at: 2499 2571 2616 2772 2499
Size: 72 45 156 5894
Fragments arranged by size:
5894 156 72 45
ApaLI G'TGCA_C
Cuts at: 829 4937 829
Size: 4108 2059
ApoI r'AATT_y
Cuts at: 3171 5520 5776 3171
Size: 2349 256 3562
Fragments arranged by size:
3562 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6167
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6167
AvaI C'yCGr_G
Cuts at: 674 1061 1104 1160 1238 2615 3187 674
Size: 387 43 56 78 1377 572 3654
Fragments arranged by size:
3654 1377 572 387 78 56 43
BamHI G'GATC_C
Cuts at: 1248 3192 1248
Size: 1944 4223
BanI G'GyrC_C
Cuts at: 302 1534 2275 3071 3183 3597 3845 3880 302
Size: 1232 741 796 112 414 248 35 2589
Fragments arranged by size:
2589 1232 796 741 414 248 112 35
BanII G_rGCy'C
Cuts at: 320 1895 2104 2229 2358 2499 2571 2616 2772
Size: 1575 209 125 129 141 72 45 156
Cuts at: 2772 3092 3113 3181 4211 320
Size: 320 21 68 1030 2276
Fragments arranged by size:
2276 1575 1030 320 209 156 141 129
125 72 68 45 21
BbeI G_GCGC'C
Cuts at: 3075 3601 3849 3075
Size: 526 248 5393
Fragments arranged by size:
5393 526 248
BbsI GAAGACnn'nnnn_
Cuts at: 436 436
Size: 6167
BbvCI CC'TCA_GC
Cuts at: 494 1145 2305 494
Size: 651 1160 4356
Fragments arranged by size:
4356 1160 651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2563 2597 2563
Size: 34 6133
BciVI GTATCCnnnnn_n'
Cuts at: 4065 4826 6167 4065
Size: 761 1341 4065
Fragments arranged by size:
4065 1341 761
BclI T'GATC_A
Cuts at: 3687 3687
Size: 6167
BglI GCCn_nnn'nGGC
Cuts at: 367 593 1021 1791 2554 2566 367
Size: 226 428 770 763 12 3968
Fragments arranged by size:
3968 770 763 428 226 12
BglII A'GATC_T
Cuts at: 3682 5719 3682
Size: 2037 4130
BlpI GC'TnA_GC
Cuts at: 1043 2366 2669 2863 3279 1043
Size: 1323 303 194 416 3931
Fragments arranged by size:
3931 1323 416 303 194
Bme1580I G_kGCm'C
Cuts at: 833 2278 2499 2571 2616 2772 2843 3792 3885
Size: 1445 221 72 45 156 71 949 93
Cuts at: 3885 4941 833
Size: 1056 2059
Fragments arranged by size:
2059 1445 1056 949 221 156 93 72
71 45
BmgBI CAC'GTC
Cuts at: 516 2157 516
Size: 1641 4526
BmrI ACTGGGnnnn_n'
Cuts at: 2601 3379 3460 3795 5939 2601
Size: 778 81 335 2144 2829
Fragments arranged by size:
2829 2144 778 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 832 1738 2248 4534 832
Size: 906 510 2286 2465
Fragments arranged by size:
2465 2286 906 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 567 685 2011 2152 2233 3043 562
Size: 5 118 1326 141 81 810 3686
Fragments arranged by size:
3686 1326 810 141 118 81 5
Bpu10I CC'TnA_GC
Cuts at: 199 494 1145 2053 2305 199
Size: 295 651 908 252 4061
Fragments arranged by size:
4061 908 651 295 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 667 2866 3343 4222 4714 5012 508
Size: 159 2199 477 879 492 298 1663
Fragments arranged by size:
2199 1663 879 492 477 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 2876 5871 2876
Size: 2995 3172
BsaAI yAC'GTr
Cuts at: 693 1682 3520 693
Size: 989 1838 3340
Fragments arranged by size:
3340 1838 989
BsaBI GATnn'nnATC
Cuts at: 6 3681 5718 6
Size: 3675 2037 455
Fragments arranged by size:
3675 2037 455
BsaHI Gr'CG_yC
Cuts at: 1676 3072 3598 3846 4548 6044 1676
Size: 1396 526 248 702 1496 1799
Fragments arranged by size:
1799 1496 1396 702 526 248
BsaWI w'CCGG_w
Cuts at: 380 1652 2336 2759 3356 3877 4555 4829 4976
Size: 1272 684 423 597 521 678 274 147
Cuts at: 4976 5387 380
Size: 411 1160
Fragments arranged by size:
1272 1160 684 678 597 521 423 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1867 1897 1867
Size: 30 6137
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 994 1033 2894 994
Size: 39 1861 4267
Fragments arranged by size:
4267 1861 39
BseYI C'CCAG_C
Cuts at: 1936 2097 3154 3592 4350 4927 5423 1936
Size: 161 1057 438 758 577 496 2680
Fragments arranged by size:
2680 1057 758 577 496 438 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 2884 2920 748
Size: 2136 36 3995
Fragments arranged by size:
3995 2136 36
BsiEI CG_ry'CG
Cuts at: 104 638 2950 3709 3755 4963 104
Size: 534 2312 759 46 1208 1308
Fragments arranged by size:
2312 1308 1208 759 534 46
BsiHKAI G_wGCw'C
Cuts at: 320 833 1076 2717 3181 3959 4149 4941 320
Size: 513 243 1641 464 778 190 792 1546
Fragments arranged by size:
1641 1546 792 778 513 464 243 190
BsmBI CGTCTCn'nnnn_
Cuts at: 967 3039 967
Size: 2072 4095
Bsp1286I G_dGCh'C
Cuts at: 320 833 1076 1895 2104 2229 2278 2358 2499
Size: 513 243 819 209 125 49 80 141
Cuts at: 2499 2571 2616 2717 2772 2843 3092 3113 3181
Size: 72 45 101 55 71 249 21 68
Cuts at: 3181 3792 3885 3959 4149 4211 4941 320
Size: 611 93 74 190 62 730 1546
Fragments arranged by size:
1546 819 730 611 513 249 243 209
190 141 125 101 93 80 74 72
71 68 62 55 49 45 21
BspEI T'CCGG_A
Cuts at: 1652 2336 2759 5387 1652
Size: 684 423 2628 2432
Fragments arranged by size:
2628 2432 684 423
BspHI T'CATG_A
Cuts at: 182 5343 182
Size: 5161 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 403 2102 2476 2477 3217 3431 3733 4114
Size: 203 1699 374 1 740 214 302 381
Cuts at: 4114 200
Size: 2253
Fragments arranged by size:
2253 1699 740 381 374 302 214 203
1
BsrBI CCG'CTC
Cuts at: 50 327 1079 1482 2189 2942 4459 4513 5997
Size: 277 752 403 707 753 1517 54 1484
Cuts at: 5997 6162 50
Size: 165 55
Fragments arranged by size:
1517 1484 753 752 707 403 277 165
55 54
BsrDI GCAATG_nn'
Cuts at: 377 561 4079 377
Size: 184 3518 2465
Fragments arranged by size:
3518 2465 184
BsrFI r'CCGG_y
Cuts at: 380 2813 4165 4346 4555 380
Size: 2433 1352 181 209 1992
Fragments arranged by size:
2433 1992 1352 209 181
BsrGI T'GTAC_A
Cuts at: 1748 1748
Size: 6167
BssSI C'ACGA_G
Cuts at: 1069 2923 4438 4796 1069
Size: 1854 1515 358 2440
Fragments arranged by size:
2440 1854 1515 358
BstAPI GCAn_nnn'nTGC
Cuts at: 453 762 2145 3544 453
Size: 309 1383 1399 3076
Fragments arranged by size:
3076 1399 1383 309
BstBI TT'CG_AA
Cuts at: 4528 4528
Size: 6167
BstEII G'GTnAC_C
Cuts at: 1812 1812
Size: 6167
BstXI CCAn_nnnn'nTGG
Cuts at: 1467 1467
Size: 6167
BstYI r'GATC_y
Cuts at: 1 1248 1470 2756 3192 3659 3682 4017 4263
Size: 1247 222 1286 436 467 23 335 246
Cuts at: 4263 5275 5361 5373 5719 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1286 1247 1012 467 449 436 346 335
246 222 86 23 12
BstZ17I GTA'TAC
Cuts at: 789 789
Size: 6167
Bsu36I CC'TnA_GG
Cuts at: 287 287
Size: 6167
BtgI C'CryG_G
Cuts at: 107 170 1491 1631 1688 1856 2561 2907 3083
Size: 63 1321 140 57 168 705 346 176
Cuts at: 3083 107
Size: 3191
Fragments arranged by size:
3191 1321 705 346 176 168 140 63
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 279 447 910 3043 4117 4298 4461 279
Size: 168 463 2133 1074 181 163 1985
Fragments arranged by size:
2133 1985 1074 463 181 168 163
BtsI GCAGTG_nn'
Cuts at: 744 875 1016 1136 1187 2997 3410 744
Size: 131 141 120 51 1810 413 3501
Fragments arranged by size:
3501 1810 413 141 131 120 51
DraI TTT'AAA
Cuts at: 3166 3166
Size: 6167
DraIII CAC_nnn'GTG
Cuts at: 2349 2349
Size: 6167
DrdI GACnn_nn'nnGTC
Cuts at: 3873 4731 3873
Size: 858 5309
EaeI y'GGCC_r
Cuts at: 328 350 1013 1052 1502 1641 1783 2166 2428
Size: 22 663 39 450 139 142 383 262
Cuts at: 2428 2555 2910 2947 2990 3752 3926 4317 4344
Size: 127 355 37 43 762 174 391 27
Cuts at: 4344 5689 328
Size: 1345 806
Fragments arranged by size:
1345 806 762 663 450 391 383 355
262 174 142 139 127 43 39 37
27 22
EagI C'GGCC_G
Cuts at: 2947 3752 2947
Size: 805 5362
EarI CTCTTCn'nnn_
Cuts at: 902 1400 1729 1849 2485 4190 4400 902
Size: 498 329 120 636 1705 210 2669
Fragments arranged by size:
2669 1705 636 498 329 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4375 4685 4831 5977 4375
Size: 310 146 1146 4565
Fragments arranged by size:
4565 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 3179 318
Size: 2861 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 567 685 882 926 1411 2011 2152 2233
Size: 5 118 197 44 485 600 141 81
Cuts at: 2233 2510 3043 3992 4424 5171 5439 562
Size: 277 533 949 432 747 268 1290
Fragments arranged by size:
1290 949 747 600 533 485 432 277
268 197 141 118 81 44 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1393 1726 2283 1393
Size: 333 557 5277
Fragments arranged by size:
5277 557 333
EcoO109I rG'GnC_Cy
Cuts at: 124 630 678 1002 1065 2567 2568 2769 3306
Size: 506 48 324 63 1502 1 201 537
Cuts at: 3306 124
Size: 2985
Fragments arranged by size:
2985 1502 537 506 324 201 63 48
1
EcoRI G'AATT_C
Cuts at: 3171 3171
Size: 6167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1911 4186 1911
Size: 2275 3892
FspI TGC'GCA
Cuts at: 3948 3948
Size: 6167
HaeII r_GCGC'y
Cuts at: 1824 2126 2295 3075 3601 3849 4871 5850 1824
Size: 302 169 780 526 248 1022 979 2141
Fragments arranged by size:
2141 1022 979 780 526 302 248 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 901 933 2036 2068 2611 2643 4009
Size: 32 237 32 1103 32 543 32 1366
Cuts at: 4009 4039 4041 4071 632
Size: 30 2 30 2728
Fragments arranged by size:
2728 1366 1103 543 237 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1320 3206 1320
Size: 1886 4281
KasI G'GCGC_C
Cuts at: 3071 3597 3845 3071
Size: 526 248 5393
Fragments arranged by size:
5393 526 248
KpnI G_GTAC'C
Cuts at: 306 3187 306
Size: 2881 3286
MfeI C'AATT_G
Cuts at: 724 724
Size: 6167
MluI A'CGCG_T
Cuts at: 5600 5600
Size: 6167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3062 4838 5022 2129
Size: 933 1776 184 3274
Fragments arranged by size:
3274 1776 933 184
MscI TGG'CCA
Cuts at: 352 2168 3928 352
Size: 1816 1760 2591
Fragments arranged by size:
2591 1816 1760
MslI CAynn'nnrTG
Cuts at: 341 535 695 826 1282 1465 1810 2245 2323
Size: 194 160 131 456 183 345 435 78
Cuts at: 2323 3082 4156 4283 341
Size: 759 1074 127 2225
Fragments arranged by size:
2225 1074 759 456 435 345 194 183
160 131 127 78
MspA1I CmG'CkG
Cuts at: 753 1144 1191 1907 2376 2433 2505 2876 2889
Size: 391 47 716 469 57 72 371 13
Cuts at: 2889 2909 3122 3278 3592 3952 4965 5210 753
Size: 20 213 156 314 360 1013 245 1710
Fragments arranged by size:
1710 1013 716 469 391 371 360 314
245 213 156 72 57 47 20 13
NaeI GCC'GGC
Cuts at: 4348 4348
Size: 6167
NarI GG'CG_CC
Cuts at: 3072 3598 3846 3072
Size: 526 248 5393
Fragments arranged by size:
5393 526 248
NcoI C'CATG_G
Cuts at: 107 170 1631 1856 2561 107
Size: 63 1461 225 705 3713
Fragments arranged by size:
3713 1461 705 225 63
NgoMIV G'CCGG_C
Cuts at: 4346 4346
Size: 6167
NspI r_CATG'y
Cuts at: 2535 3220 3720 4627 2535
Size: 685 500 907 4075
Fragments arranged by size:
4075 907 685 500
PciI A'CATG_T
Cuts at: 4623 4623
Size: 6167
PflMI CCAn_nnn'nTGG
Cuts at: 2097 2892 2097
Size: 795 5372
PfoI T'CCnGG_A
Cuts at: 1727 2129 2459 1727
Size: 402 330 5435
Fragments arranged by size:
5435 402 330
PmeI GTTT'AAAC
Cuts at: 3166 3166
Size: 6167
PmlI CAC'GTG
Cuts at: 693 693
Size: 6167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1899 5306 5338 1867
Size: 32 3407 32 2696
Fragments arranged by size:
3407 2696 32 32
PpuMI rG'GwC_Cy
Cuts at: 630 678 1065 630
Size: 48 387 5732
Fragments arranged by size:
5732 387 48
PshAI GACnn'nnGTC
Cuts at: 2203 2203
Size: 6167
PspOMI G'GGCC_C
Cuts at: 2495 2567 2612 2768 2495
Size: 72 45 156 5894
Fragments arranged by size:
5894 156 72 45
PstI C_TGCA'G
Cuts at: 197 995 1143 1367 1525 1562 2150 2375 2453
Size: 798 148 224 158 37 588 225 78
Cuts at: 2453 2488 3214 3899 197
Size: 35 726 685 2465
Fragments arranged by size:
2465 798 726 685 588 225 224 158
148 78 37 35
PvuI CG_AT'CG
Cuts at: 104 638 104
Size: 534 5633
PvuII CAG'CTG
Cuts at: 753 1144 2505 2889 3592 3952 753
Size: 391 1361 384 703 360 2968
Fragments arranged by size:
2968 1361 703 391 384 360
RsrII CG'GwC_CG
Cuts at: 4362 4362
Size: 6167
SacI G_AGCT'C
Cuts at: 320 3181 320
Size: 2861 3306
SacII CC_GC'GG
Cuts at: 2910 2910
Size: 6167
SalI G'TCGA_C
Cuts at: 3204 3204
Size: 6167
SanDI GG'GwC_CC
Cuts at: 630 630
Size: 6167
SapI GCTCTTCn'nnn_
Cuts at: 4190 4400 4190
Size: 210 5957
SbfI CC_TGCA'GG
Cuts at: 3214 3214
Size: 6167
ScaI AGT'ACT
Cuts at: 5684 5684
Size: 6167
SexAI A'CCwGG_T
Cuts at: 1967 1967
Size: 6167
SfcI C'TryA_G
Cuts at: 33 193 991 1139 1363 1521 1558 2146 2371
Size: 160 798 148 224 158 37 588 225
Cuts at: 2371 2449 2484 3210 3895 4888 5079 33
Size: 78 35 726 685 993 191 1121
Fragments arranged by size:
1121 993 798 726 685 588 225 224
191 160 158 148 78 37 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 2554 2554
Size: 6167
SfoI GGC'GCC
Cuts at: 3073 3599 3847 3073
Size: 526 248 5393
Fragments arranged by size:
5393 526 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6167
SmaI CCC'GGG
Cuts at: 1063 1162 1240 3189 1063
Size: 99 78 1949 4041
Fragments arranged by size:
4041 1949 99 78
SmlI C'TyrA_G
Cuts at: 523 682 2845 3322 4237 4729 4991 523
Size: 159 2163 477 915 492 262 1699
Fragments arranged by size:
2163 1699 915 492 477 262 159
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6167
SphI G_CATG'C
Cuts at: 3220 3720 3220
Size: 500 5667
StuI AGG'CCT
Cuts at: 547 1519 547
Size: 972 5195
StyI C'CwwG_G
Cuts at: 107 170 839 1175 1631 1856 1886 2279 2549
Size: 63 669 336 456 225 30 393 270
Cuts at: 2549 2561 3301 3655 107
Size: 12 740 354 2619
Fragments arranged by size:
2619 740 669 456 393 354 336 270
225 63 30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 685 889 1194 1562 3750 3883 4342 4551 685
Size: 204 305 368 2188 133 459 209 2301
Fragments arranged by size:
2301 2188 459 368 305 209 204 133
TatI w'GTAC_w
Cuts at: 458 599 1748 5682 458
Size: 141 1149 3934 943
Fragments arranged by size:
3934 1149 943 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1209 1241 2628 2660 5684 5716 1209
Size: 32 1387 32 3024 32 1660
Fragments arranged by size:
3024 1660 1387 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1273 3964 1273
Size: 2691 3476
XbaI T'CTAG_A
Cuts at: 3198 3198
Size: 6167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 948 1470 1863 2094 3662 537
Size: 411 522 393 231 1568 3042
Fragments arranged by size:
3042 1568 522 411 393 231
XmaI C'CCGG_G
Cuts at: 1061 1160 1238 3187 1061
Size: 99 78 1949 4041
Fragments arranged by size:
4041 1949 99 78
XmnI GAAnn'nnTTC
Cuts at: 2746 2746
Size: 6167
ZraI GAC'GTC
Cuts at: 1677 1677
Size: 6167
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BsiWI BsmI
BssHII ClaI CspCI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspXI PsrI SgrAI SnaBI SrfI SspI SwaI XhoI