Restriction map for
pF1KF0316
(Circular) (Six-base) MAPSORT of: pF1KF0316.seq Check: 7619 from: 1 to: 5033
pF1KF0316 5033 bp
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AccI GT'mk_AC
Cuts at: 2071 2071
Size: 5033
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 4873 4873
Size: 5033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2858 3290 3643 4305 1831
Size: 1027 432 353 662 2559
Fragments arranged by size:
2559 1027 662 432 353
AflII C'TTAA_G
Cuts at: 1381 1831 1381
Size: 450 4583
AflIII A'CryG_T
Cuts at: 2385 4185 4466 2385
Size: 1800 281 2952
Fragments arranged by size:
2952 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 636 636
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 160 567 1129 1176 1206 1662 2338 3776 160
Size: 407 562 47 30 456 676 1438 1417
Fragments arranged by size:
1438 1417 676 562 456 407 47 30
ApaLI G'TGCA_C
Cuts at: 3871 3871
Size: 5033
ApoI r'AATT_y
Cuts at: 222 791 798 1285 1424 1840 2037 4386 4642
Size: 569 7 487 139 416 197 2349 256
Cuts at: 4642 222
Size: 613
Fragments arranged by size:
2349 613 569 487 416 256 197 139
7
AseI AT'TA_AT
Cuts at: 20 1877 20
Size: 1857 3176
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 2053 2053
Size: 5033
BamHI G'GATC_C
Cuts at: 2058 2058
Size: 5033
BanI G'GyrC_C
Cuts at: 2049 2463 2711 2746 2049
Size: 414 248 35 4336
Fragments arranged by size:
4336 414 248 35
BanII G_rGCy'C
Cuts at: 358 2047 3077 358
Size: 1689 1030 2314
Fragments arranged by size:
2314 1689 1030
BbeI G_GCGC'C
Cuts at: 2467 2715 2467
Size: 248 4785
BbsI GAAGACnn'nnnn_
Cuts at: 1242 1242
Size: 5033
BbvCI CC'TCA_GC
Cuts at: 641 641
Size: 5033
BciVI GTATCCnnnnn_n'
Cuts at: 2931 3987 5033 2931
Size: 1056 1046 2931
Fragments arranged by size:
2931 1056 1046
BclI T'GATC_A
Cuts at: 2553 2553
Size: 5033
BglII A'GATC_T
Cuts at: 2548 4585 2548
Size: 2037 2996
BlpI GC'TnA_GC
Cuts at: 1376 2145 1376
Size: 769 4264
Bme1580I G_kGCm'C
Cuts at: 140 404 1502 2658 2751 3875 140
Size: 264 1098 1156 93 1124 1298
Fragments arranged by size:
1298 1156 1124 1098 264 93
BmgBI CAC'GTC
Cuts at: 1215 1215
Size: 5033
BmrI ACTGGGnnnn_n'
Cuts at: 2245 2326 2661 4805 2245
Size: 81 335 2144 2473
Fragments arranged by size:
2473 2144 335 81
BmtI G_CTAG'C
Cuts at: 598 1952 598
Size: 1354 3679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 633 727 772 1105 1183 1460 1516 1622
Size: 44 94 45 333 78 277 56 106
Cuts at: 1622 589
Size: 4000
Fragments arranged by size:
4000 333 277 106 94 78 56 45
44
Bpu10I CC'TnA_GC
Cuts at: 641 641
Size: 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1691 1726 2209 3088 3802 4100 1135
Size: 556 35 483 879 714 298 2068
Fragments arranged by size:
2068 879 714 556 483 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 4737 4737
Size: 5033
BsaAI yAC'GTr
Cuts at: 2023 2386 2023
Size: 363 4670
BsaBI GATnn'nnATC
Cuts at: 6 2547 4584 6
Size: 2541 2037 455
Fragments arranged by size:
2541 2037 455
BsaHI Gr'CG_yC
Cuts at: 2464 2712 3414 4910 2464
Size: 248 702 1496 2587
Fragments arranged by size:
2587 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2222 2743 3421 3832 3979 4253 2222
Size: 521 678 411 147 274 3002
Fragments arranged by size:
3002 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 1166 1196 1319 1349 700
Size: 30 436 30 123 30 4384
Fragments arranged by size:
4384 436 123 30 30 30
BseYI C'CCAG_C
Cuts at: 1026 1109 1122 1169 2458 3216 3881 4289 1026
Size: 83 13 47 1289 758 665 408 1770
Fragments arranged by size:
1770 1289 758 665 408 83 47 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 562 1153 385
Size: 177 591 4265
Fragments arranged by size:
4265 591 177
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3851 104
Size: 2471 46 1230 1286
Fragments arranged by size:
2471 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 550 1262 1400 2047 2825 3015 3875 550
Size: 712 138 647 778 190 860 1708
Fragments arranged by size:
1708 860 778 712 647 190 138
BsmI GAATG_Cn'
Cuts at: 762 1095 762
Size: 333 4700
Bsp1286I G_dGCh'C
Cuts at: 140 358 404 550 1262 1400 1502 2047 2658
Size: 218 46 146 712 138 102 545 611
Cuts at: 2658 2751 2825 3015 3077 3875 140
Size: 93 74 190 62 798 1298
Fragments arranged by size:
1298 798 712 611 545 218 190 146
138 102 93 74 62 46
BspHI T'CATG_A
Cuts at: 170 314 3465 170
Size: 144 3151 1738
Fragments arranged by size:
3151 1738 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2083 2297 2599 2980 2083
Size: 214 302 381 4136
Fragments arranged by size:
4136 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1626 3325 3379 4863 5028 50
Size: 1576 1699 54 1484 165 55
Fragments arranged by size:
1699 1576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 1241 2945 345
Size: 896 1704 2433
Fragments arranged by size:
2433 1704 896
BsrFI r'CCGG_y
Cuts at: 863 3031 3212 863
Size: 2168 181 2684
Fragments arranged by size:
2684 2168 181
BssSI C'ACGA_G
Cuts at: 489 3304 4012 489
Size: 2815 708 1510
Fragments arranged by size:
2815 1510 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2410 2410
Size: 5033
BstBI TT'CG_AA
Cuts at: 3394 3394
Size: 5033
BstXI CCAn_nnnn'nTGG
Cuts at: 174 174
Size: 5033
BstYI r'GATC_y
Cuts at: 1 494 1934 2058 2525 2548 2883 3129 3435
Size: 493 1440 124 467 23 335 246 306
Cuts at: 3435 3447 3533 4585 1
Size: 12 86 1052 449
Fragments arranged by size:
1440 1052 493 467 449 335 306 246
124 86 23 12
BtgI C'CryG_G
Cuts at: 107 263 359 107
Size: 156 96 4781
Fragments arranged by size:
4781 156 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 473 2983 3164 3327 473
Size: 2510 181 163 2179
Fragments arranged by size:
2510 2179 181 163
BtsI GCAGTG_nn'
Cuts at: 140 1100 1595 2276 140
Size: 960 495 681 2897
Fragments arranged by size:
2897 960 681 495
DraI TTT'AAA
Cuts at: 890 2032 890
Size: 1142 3891
DraIII CAC_nnn'GTG
Cuts at: 585 585
Size: 5033
DrdI GACnn_nn'nnGTC
Cuts at: 2739 4083 2739
Size: 1344 3689
EaeI y'GGCC_r
Cuts at: 275 2618 2792 3183 3210 4555 275
Size: 2343 174 391 27 1345 753
Fragments arranged by size:
2343 1345 753 391 174 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 128 200 1343 3056 3266 128
Size: 72 1143 1713 210 1895
Fragments arranged by size:
1895 1713 1143 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3241 3983 4129 4843 3241
Size: 742 146 714 3431
Fragments arranged by size:
3431 742 714 146
EcoICRI GAG'CTC
Cuts at: 2045 2045
Size: 5033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 633 727 772 1105 1183 1460 1516 1622
Size: 44 94 45 333 78 277 56 106
Cuts at: 1622 1831 2858 3290 3643 4305 589
Size: 209 1027 432 353 662 1317
Fragments arranged by size:
1317 1027 662 432 353 333 277 209
106 94 78 56 45 44
EcoNI CCTnn'n_nnAGG
Cuts at: 997 1230 997
Size: 233 4800
EcoO109I rG'GnC_Cy
Cuts at: 450 564 1527 2172 450
Size: 114 963 645 3311
Fragments arranged by size:
3311 963 645 114
EcoRI G'AATT_C
Cuts at: 222 1285 2037 222
Size: 1063 752 3218
Fragments arranged by size:
3218 1063 752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1347 1846 3052 1347
Size: 499 1206 3328
Fragments arranged by size:
3328 1206 499
FspI TGC'GCA
Cuts at: 2814 2814
Size: 5033
HaeII r_GCGC'y
Cuts at: 2467 2715 3945 4716 2467
Size: 248 1230 771 2784
Fragments arranged by size:
2784 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 806 838 989 1021 1436 1468 1689 1721 1953
Size: 32 151 32 415 32 221 32 232
Cuts at: 1953 1985 1995 2027 2875 2905 2907 2937 806
Size: 32 10 32 848 30 2 30 2902
Fragments arranged by size:
2902 848 415 232 221 151 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 2072 2072
Size: 5033
HindIII A'AGCT_T
Cuts at: 1297 1716 1297
Size: 419 4614
KasI G'GCGC_C
Cuts at: 2463 2711 2463
Size: 248 4785
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3792 3976 3792
Size: 184 4849
MscI TGG'CCA
Cuts at: 277 2794 277
Size: 2517 2516
MslI CAynn'nnrTG
Cuts at: 172 388 3022 3149 172
Size: 216 2634 127 2056
Fragments arranged by size:
2634 2056 216 127
MspA1I CmG'CkG
Cuts at: 213 576 1126 1621 1645 2144 2458 2818 3602
Size: 363 550 495 24 499 314 360 784
Cuts at: 3602 3847 213
Size: 245 1399
Fragments arranged by size:
1399 784 550 499 495 363 360 314
245 24
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5033
NarI GG'CG_CC
Cuts at: 2464 2712 2464
Size: 248 4785
NcoI C'CATG_G
Cuts at: 107 107
Size: 5033
NdeI CA'TA_TG
Cuts at: 896 896
Size: 5033
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5033
NheI G'CTAG_C
Cuts at: 594 1948 594
Size: 1354 3679
NspI r_CATG'y
Cuts at: 810 1222 2086 2586 4189 810
Size: 412 864 500 1603 1654
Fragments arranged by size:
1654 1603 864 500 412
PciI A'CATG_T
Cuts at: 4185 4185
Size: 5033
PflMI CCAn_nnn'nTGG
Cuts at: 429 567 1164 429
Size: 138 597 4298
Fragments arranged by size:
4298 597 138
PfoI T'CCnGG_A
Cuts at: 1199 1437 1721 1199
Size: 238 284 4511
Fragments arranged by size:
4511 284 238
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 5001
PpuMI rG'GwC_Cy
Cuts at: 1527 1527
Size: 5033
PstI C_TGCA'G
Cuts at: 135 1343 1547 1580 2080 2765 135
Size: 1208 204 33 500 685 2403
Fragments arranged by size:
2403 1208 685 500 204 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 213 576 1645 2458 2818 213
Size: 363 1069 813 360 2428
Fragments arranged by size:
2428 1069 813 363 360
RsrII CG'GwC_CG
Cuts at: 3228 3228
Size: 5033
SacI G_AGCT'C
Cuts at: 2047 2047
Size: 5033
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 200 3056 3266 200
Size: 2856 210 1967
Fragments arranged by size:
2856 1967 210
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 4550 4550
Size: 5033
SexAI A'CCwGG_T
Cuts at: 144 144
Size: 5033
SfcI C'TryA_G
Cuts at: 33 131 1152 1339 1543 1576 2076 2761 3729
Size: 98 1021 187 204 33 500 685 968
Cuts at: 3729 3920 33
Size: 191 1146
Fragments arranged by size:
1146 1021 968 685 500 204 191 187
98 33
SfoI GGC'GCC
Cuts at: 2465 2713 2465
Size: 248 4785
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 2055 2055
Size: 5033
SmlI C'TyrA_G
Cuts at: 1114 1381 1706 1741 1831 2188 3103 3817 4079
Size: 267 325 35 90 357 915 714 262
Cuts at: 4079 1114
Size: 2068
Fragments arranged by size:
2068 915 714 357 325 267 262 90
35
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 1222 2086 2586 1222
Size: 864 500 3669
Fragments arranged by size:
3669 864 500
SspI AAT'ATT
Cuts at: 886 1366 886
Size: 480 4553
StyI C'CwwG_G
Cuts at: 107 2167 2521 107
Size: 2060 354 2619
Fragments arranged by size:
2619 2060 354
SwaI ATTT'AAAT
Cuts at: 890 890
Size: 5033
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 988 2616 2749 3208 3417 100
Size: 888 1628 133 459 209 1716
Fragments arranged by size:
1716 1628 888 459 209 133
TatI w'GTAC_w
Cuts at: 4548 4548
Size: 5033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 381 413 1319 1351 4550 4582 381
Size: 32 906 32 3199 32 832
Fragments arranged by size:
3199 906 832 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2830 2830
Size: 5033
XbaI T'CTAG_A
Cuts at: 2064 2064
Size: 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 680 2528 189
Size: 491 1848 2694
Fragments arranged by size:
2694 1848 491
XmaI C'CCGG_G
Cuts at: 2053 2053
Size: 5033
XmnI GAAnn'nnTTC
Cuts at: 1772 1841 1772
Size: 69 4964
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BseRI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
XhoI ZraI