Restriction map for
pF1KF0343
(Circular) (Six-base) MAPSORT of: pF1KF0343.seq Check: 3630 from: 1 to: 11030
pF1KF0343 11030 bp
With 182 enzymes: SgfI *
September 25, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3840 3840
Size: 11030
AatII G_ACGT'C
Cuts at: 2519 2834 7295 2519
Size: 315 4461 6254
Fragments arranged by size:
6254 4461 315
AccI GT'mk_AC
Cuts at: 170 2429 4607 4918 7688 7823 8068 170
Size: 2259 2178 311 2770 135 245 3132
Fragments arranged by size:
3132 2770 2259 2178 311 245 135
Acc65I G'GTAC_C
Cuts at: 4695 8046 4695
Size: 3351 7679
AclI AA'CG_TT
Cuts at: 6548 10870 6548
Size: 4322 6708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 529 654 934 1672 2367 6100 6521 6857
Size: 231 125 280 738 695 3733 421 336
Cuts at: 6857 8855 9287 9640 10302 298
Size: 1998 432 353 662 1026
Fragments arranged by size:
3733 1998 1026 738 695 662 432 421
353 336 280 231 125
AflIII A'CryG_T
Cuts at: 425 3469 8382 10182 10463 425
Size: 3044 4913 1800 281 992
Fragments arranged by size:
4913 3044 1800 992 281
AleI CACnn'nnGTG
Cuts at: 4783 7966 4783
Size: 3183 7847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4141 7864 4141
Size: 3723 7307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2821 2853 5894 5926 2821
Size: 32 3041 32 7925
Fragments arranged by size:
7925 3041 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1339 5056 5352 6866 8335 9773 1339
Size: 3717 296 1514 1469 1438 2596
Fragments arranged by size:
3717 2596 1514 1469 1438 296
ApaI G_GGCC'C
Cuts at: 699 1672 1858 2746 3139 3227 3427 4291 4475
Size: 973 186 888 393 88 200 864 184
Cuts at: 4475 4577 4661 5400 5797 6343 6779 6820 6916
Size: 102 84 739 397 546 436 41 96
Cuts at: 6916 7731 699
Size: 815 3998
Fragments arranged by size:
3998 973 888 864 815 739 546 436
397 393 200 186 184 102 96 88
84 41
ApaLI G'TGCA_C
Cuts at: 1474 5040 5139 5148 7186 9868 1474
Size: 3566 99 9 2038 2682 2636
Fragments arranged by size:
3566 2682 2636 2038 99 9
ApoI r'AATT_y
Cuts at: 6856 8034 10383 10639 6856
Size: 1178 2349 256 7247
Fragments arranged by size:
7247 2349 1178 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11030
AvaI C'yCGr_G
Cuts at: 125 439 726 838 1150 1415 1545 2627 3141
Size: 314 287 112 312 265 130 1082 514
Cuts at: 3141 3442 3499 3610 3663 3784 3953 4175 4765
Size: 301 57 111 53 121 169 222 590
Cuts at: 4765 5933 6054 6872 7265 7731 8050 125
Size: 1168 121 818 393 466 319 3105
Fragments arranged by size:
3105 1168 1082 818 590 514 466 393
319 314 312 301 287 265 222 169
130 121 121 112 111 57 53
AvrII C'CTAG_G
Cuts at: 6780 6780
Size: 11030
BamHI G'GATC_C
Cuts at: 632 2967 4863 8055 632
Size: 2335 1896 3192 3607
Fragments arranged by size:
3607 3192 2335 1896
BanI G'GyrC_C
Cuts at: 703 1819 3153 3388 4146 4210 4366 4621 4695
Size: 1116 1334 235 758 64 156 255 74
Cuts at: 4695 5560 7050 7306 7462 7554 8019 8046 8460
Size: 865 1490 256 156 92 465 27 414
Cuts at: 8460 8708 8743 703
Size: 248 35 2990
Fragments arranged by size:
2990 1490 1334 1116 865 758 465 414
256 255 248 235 156 156 92 74
64 35 27
BanII G_rGCy'C
Cuts at: 699 927 986 1213 1286 1672 1858 2459 2474
Size: 228 59 227 73 386 186 601 15
Cuts at: 2474 2711 2746 2986 3139 3227 3358 3427 3500
Size: 237 35 240 153 88 131 69 73
Cuts at: 3500 3960 4291 4475 4486 4577 4661 4708 4772
Size: 460 331 184 11 91 84 47 64
Cuts at: 4772 5400 5797 6071 6323 6343 6534 6757 6779
Size: 628 397 274 252 20 191 223 22
Cuts at: 6779 6798 6820 6916 7206 7731 8044 9074 699
Size: 19 22 96 290 525 313 1030 2655
Fragments arranged by size:
2655 1030 628 601 525 460 397 386
331 313 290 274 252 240 237 228
227 223 191 186 184 153 131 96
91 88 84 73 73 69 64 59
47 35 22 22 20 19 15 11
BbeI G_GCGC'C
Cuts at: 707 3392 8464 8712 707
Size: 2685 5072 248 3025
Fragments arranged by size:
5072 3025 2685 248
BbsI GAAGACnn'nnnn_
Cuts at: 2069 2164 3909 4891 2069
Size: 95 1745 982 8208
Fragments arranged by size:
8208 1745 982 95
BbvCI CC'TCA_GC
Cuts at: 1403 2074 3019 6568 1403
Size: 671 945 3549 5865
Fragments arranged by size:
5865 3549 945 671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 279 313 474 508 1785 1819 2748 2782 279
Size: 34 161 34 1277 34 929 34 8527
Fragments arranged by size:
8527 1277 929 161 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8928 9984 11030 8928
Size: 1056 1046 8928
Fragments arranged by size:
8928 1056 1046
BclI T'GATC_A
Cuts at: 830 5096 6197 8550 830
Size: 4266 1101 2353 3310
Fragments arranged by size:
4266 3310 2353 1101
BglI GCCn_nnn'nGGC
Cuts at: 1558 3997 6629 7168 7526 1558
Size: 2439 2632 539 358 5062
Fragments arranged by size:
5062 2632 2439 539 358
BglII A'GATC_T
Cuts at: 1322 3695 6929 8545 10582 1322
Size: 2373 3234 1616 2037 1770
Fragments arranged by size:
3234 2373 2037 1770 1616
BlpI GC'TnA_GC
Cuts at: 358 6322 7783 8142 358
Size: 5964 1461 359 3246
Fragments arranged by size:
5964 3246 1461 359
Bme1580I G_kGCm'C
Cuts at: 699 726 1393 1478 1499 1672 1822 1858 1930
Size: 27 667 85 21 173 150 36 72
Cuts at: 1930 2318 2541 2746 3139 3158 3227 3260 3427
Size: 388 223 205 393 19 69 33 167
Cuts at: 3427 3491 3830 4130 4151 4291 4475 4577 4646
Size: 64 339 300 21 140 184 102 69
Cuts at: 4646 4661 5021 5044 5143 5152 5279 5400 5797
Size: 15 360 23 99 9 127 121 397
Cuts at: 5797 6122 6343 6709 6779 6820 6916 7190 7467
Size: 325 221 366 70 41 96 274 277
Cuts at: 7467 7522 7716 7731 8024 8655 8748 9872 699
Size: 55 194 15 293 631 93 1124 1857
Fragments arranged by size:
1857 1124 667 631 397 393 388 366
360 339 325 300 293 277 274 223
221 205 194 184 173 167 150 140
127 121 102 99 96 93 85 72
70 69 69 64 55 41 36 33
27 23 21 21 19 15 15 9
BmgBI CAC'GTC
Cuts at: 1479 1923 3984 4104 4602 4941 5138 5181 5910
Size: 444 2061 120 498 339 197 43 729
Cuts at: 5910 6386 1479
Size: 476 6123
Fragments arranged by size:
6123 2061 729 498 476 444 339 197
120 43
BmrI ACTGGGnnnn_n'
Cuts at: 753 888 1280 8242 8323 8658 10802 753
Size: 135 392 6962 81 335 2144 981
Fragments arranged by size:
6962 2144 981 392 335 135 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3717 4308 9397 3717
Size: 591 5089 5350
Fragments arranged by size:
5350 5089 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 1429 1561 1885 2564 3637 6394 6847 6863
Size: 129 132 324 679 1073 2757 453 16
Cuts at: 6863 6908 7228 1300
Size: 45 320 5102
Fragments arranged by size:
5102 2757 1073 679 453 324 320 132
129 45 16
Bpu10I CC'TnA_GC
Cuts at: 319 1403 2074 3019 3603 4480 6568 319
Size: 1084 671 945 584 877 2088 4781
Fragments arranged by size:
4781 2088 1084 945 877 671 584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 2443 7927 8206 9085 9799 10097 439
Size: 2004 5484 279 879 714 298 1372
Fragments arranged by size:
5484 2004 1372 879 714 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 1021 4018 4237 5348 6243 10734 1021
Size: 2997 219 1111 895 4491 1317
Fragments arranged by size:
4491 2997 1317 1111 895 219
BsaAI yAC'GTr
Cuts at: 1065 1227 1772 2046 2140 2313 3486 3567 4916
Size: 162 545 274 94 173 1173 81 1349
Cuts at: 4916 4932 5274 6102 6171 6456 6744 7797 7908
Size: 16 342 828 69 285 288 1053 111
Cuts at: 7908 8383 1065
Size: 475 3712
Fragments arranged by size:
3712 1349 1173 1053 828 545 475 342
288 285 274 173 162 111 94 81
69 16
BsaBI GATnn'nnATC
Cuts at: 6 5611 8544 10581 6
Size: 5605 2933 2037 455
Fragments arranged by size:
5605 2933 2037 455
BsaHI Gr'CG_yC
Cuts at: 167 182 704 758 1247 1463 1874 2360 2516
Size: 15 522 54 489 216 411 486 156
Cuts at: 2516 2720 2831 2945 3389 3950 4829 5030 6525
Size: 204 111 114 444 561 879 201 1495
Cuts at: 6525 7076 7292 7751 8461 8709 9411 10907 167
Size: 551 216 459 710 248 702 1496 290
Fragments arranged by size:
1496 1495 879 710 702 561 551 522
489 486 459 444 411 290 248 216
216 204 201 156 114 111 54 15
BsaWI w'CCGG_w
Cuts at: 3874 3970 8219 8740 9418 9829 9976 10250 3874
Size: 96 4249 521 678 411 147 274 4654
Fragments arranged by size:
4654 4249 678 521 411 274 147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 2290 2320 3038 3068 7474 7504 7558
Size: 30 1719 30 718 30 4406 30 54
Cuts at: 7558 7588 541
Size: 30 3983
Fragments arranged by size:
4406 3983 1719 718 54 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 595 604 856 1105 1696 4096 5101 6079
Size: 3 9 252 249 591 2400 1005 978
Cuts at: 6079 7225 7903 592
Size: 1146 678 3719
Fragments arranged by size:
3719 2400 1146 1005 978 678 591 252
249 9 3
BseYI C'CCAG_C
Cuts at: 813 1932 3631 3838 4129 4645 5228 5308 5584
Size: 1119 1699 207 291 516 583 80 276
Cuts at: 5584 6343 6811 6916 7422 7718 8455 9213 9878
Size: 759 468 105 506 296 737 758 665
Cuts at: 9878 10286 813
Size: 408 1557
Fragments arranged by size:
1699 1557 1119 759 758 737 665 583
516 506 468 408 296 291 276 207
105 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 3167 4075 5347 5584 5802 6408 529
Size: 2638 908 1272 237 218 606 5151
Fragments arranged by size:
5151 2638 1272 908 606 237 218
BsiEI CG_ry'CG
Cuts at: 104 693 1079 3722 4343 7175 8572 8618 9848
Size: 589 386 2643 621 2832 1397 46 1230
Cuts at: 9848 104
Size: 1286
Fragments arranged by size:
2832 2643 1397 1286 1230 621 589 386
46
BsiHKAI G_wGCw'C
Cuts at: 275 359 872 1184 1478 3604 4468 5044 5143
Size: 84 513 312 294 2126 864 576 99
Cuts at: 5143 5152 5273 6140 7190 7991 8044 8822 9012
Size: 9 121 867 1050 801 53 778 190
Cuts at: 9012 9872 275
Size: 860 1433
Fragments arranged by size:
2126 1433 1050 867 864 860 801 778
576 513 312 294 190 121 99 84
53 9
BsmI GAATG_Cn'
Cuts at: 5882 5882
Size: 11030
BsmBI CGTCTCn'nnnn_
Cuts at: 5083 6007 6121 7648 5083
Size: 924 114 1527 8465
Fragments arranged by size:
8465 1527 924 114
Bsp1286I G_dGCh'C
Cuts at: 275 359 699 726 872 927 986 1184 1213
Size: 84 340 27 146 55 59 198 29
Cuts at: 1213 1286 1393 1478 1499 1672 1822 1858 1930
Size: 73 107 85 21 173 150 36 72
Cuts at: 1930 2318 2459 2474 2541 2711 2746 2986 3139
Size: 388 141 15 67 170 35 240 153
Cuts at: 3139 3158 3227 3260 3358 3427 3491 3500 3604
Size: 19 69 33 98 69 64 9 104
Cuts at: 3604 3830 3960 4130 4151 4291 4468 4475 4486
Size: 226 130 170 21 140 177 7 11
Cuts at: 4486 4577 4646 4661 4708 4772 5021 5044 5143
Size: 91 69 15 47 64 249 23 99
Cuts at: 5143 5152 5273 5279 5400 5797 6071 6122 6140
Size: 9 121 6 121 397 274 51 18
Cuts at: 6140 6323 6343 6534 6709 6757 6779 6798 6820
Size: 183 20 191 175 48 22 19 22
Cuts at: 6820 6916 7190 7206 7467 7522 7716 7731 7991
Size: 96 274 16 261 55 194 15 260
Cuts at: 7991 8024 8044 8655 8748 8822 9012 9074 9872
Size: 33 20 611 93 74 190 62 798
Cuts at: 9872 275
Size: 1433
Fragments arranged by size:
1433 798 611 397 388 340 274 274
261 260 249 240 226 198 194 191
190 183 177 175 173 170 170 153
150 146 141 140 130 121 121 107
104 99 98 96 93 91 85 84
74 73 72 69 69 69 67 64
64 62 59 55 55 51 48 47
36 35 33 33 29 27 23 22
22 21 21 20 20 19 19 18
16 15 15 15 11 9 9 7
6
BspHI T'CATG_A
Cuts at: 878 2715 4734 9462 878
Size: 1837 2019 4728 2446
Fragments arranged by size:
4728 2446 2019 1837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 314 3840 3867 4548 5864 7384 7946 8080 8294
Size: 3526 27 681 1316 1520 562 134 214
Cuts at: 8294 8596 8977 314
Size: 302 381 2367
Fragments arranged by size:
3526 2367 1520 1316 681 562 381 302
214 134 27
BsrBI CCG'CTC
Cuts at: 50 689 3621 9322 9376 10860 11025 50
Size: 639 2932 5701 54 1484 165 55
Fragments arranged by size:
5701 2932 1484 639 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2295 2598 4552 6899 7455 8942 2295
Size: 303 1954 2347 556 1487 4383
Fragments arranged by size:
4383 2347 1954 1487 556 303
BsrFI r'CCGG_y
Cuts at: 192 1489 2325 3391 7916 9028 9209 192
Size: 1297 836 1066 4525 1112 181 2013
Fragments arranged by size:
4525 2013 1297 1112 1066 836 181
BsrGI T'GTAC_A
Cuts at: 1622 3728 4055 1622
Size: 2106 327 8597
Fragments arranged by size:
8597 2106 327
BssSI C'ACGA_G
Cuts at: 2467 5602 7443 7666 9301 10009 2467
Size: 3135 1841 223 1635 708 3488
Fragments arranged by size:
3488 3135 1841 1635 708 223
BstAPI GCAn_nnn'nTGC
Cuts at: 5147 6144 8407 5147
Size: 997 2263 7770
Fragments arranged by size:
7770 2263 997
BstBI TT'CG_AA
Cuts at: 1805 9391 1805
Size: 7586 3444
BstEII G'GTnAC_C
Cuts at: 1656 3240 6393 6615 7473 1656
Size: 1584 3153 222 858 5213
Fragments arranged by size:
5213 3153 1584 858 222
BstXI CCAn_nnnn'nTGG
Cuts at: 3132 3544 3748 3889 4584 6192 6351 7318 7761
Size: 412 204 141 695 1608 159 967 443
Cuts at: 7761 7776 3132
Size: 15 6386
Fragments arranged by size:
6386 1608 967 695 443 412 204 159
141 15
BstYI r'GATC_y
Cuts at: 1 236 632 1322 2967 3695 4863 4985 6018
Size: 235 396 690 1645 728 1168 122 1033
Cuts at: 6018 6929 7334 7892 8055 8522 8545 8880 9126
Size: 911 405 558 163 467 23 335 246
Cuts at: 9126 9432 9444 9530 10582 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1645 1168 1052 1033 911 728 690 558
467 449 405 396 335 306 246 235
163 122 86 23 12
BstZ17I GTA'TAC
Cuts at: 2430 4919 2430
Size: 2489 8541
Bsu36I CC'TnA_GG
Cuts at: 4008 5446 5517 7197 7523 4008
Size: 1438 71 1680 326 7515
Fragments arranged by size:
7515 1680 1438 326 71
BtgI C'CryG_G
Cuts at: 226 1376 1454 1739 2342 3741 4041 4097 4188
Size: 1150 78 285 603 1399 300 56 91
Cuts at: 4188 4802 5712 6770 7550 226
Size: 614 910 1058 780 3706
Fragments arranged by size:
3706 1399 1150 1058 910 780 614 603
300 285 91 78 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 282 1185 1281 1940 4061 6317 8980 9161 9324
Size: 903 96 659 2121 2256 2663 181 163
Cuts at: 9324 282
Size: 1988
Fragments arranged by size:
2663 2256 2121 1988 903 659 181 163
96
BtsI GCAGTG_nn'
Cuts at: 1647 2870 3354 5687 5927 8273 1647
Size: 1223 484 2333 240 2346 4404
Fragments arranged by size:
4404 2346 2333 1223 484 240
ClaI AT'CG_AT
Cuts at: 4817 7175 4817
Size: 2358 8672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1299 1334 2193 2228 4956 4991 1299
Size: 35 859 35 2728 35 7338
Fragments arranged by size:
7338 2728 859 35 35 35
DraI TTT'AAA
Cuts at: 8029 8029
Size: 11030
DraIII CAC_nnn'GTG
Cuts at: 1243 1473 1826 4098 5147 6144 1243
Size: 230 353 2272 1049 997 6129
Fragments arranged by size:
6129 2272 1049 997 353 230
DrdI GACnn_nn'nnGTC
Cuts at: 888 4449 7000 8736 10080 888
Size: 3561 2551 1736 1344 1838
Fragments arranged by size:
3561 2551 1838 1736 1344
EaeI y'GGCC_r
Cuts at: 194 690 1041 1076 1191 1530 1566 2014 2168
Size: 496 351 35 115 339 36 448 154
Cuts at: 2168 2505 3719 3816 3998 4395 5382 5679 5783
Size: 337 1214 97 182 397 987 297 104
Cuts at: 5783 6035 6096 6162 6173 6542 6896 7766 8615
Size: 252 61 66 11 369 354 870 849
Cuts at: 8615 8789 9180 9207 10552 194
Size: 174 391 27 1345 672
Fragments arranged by size:
1345 1214 987 870 849 672 496 448
397 391 369 354 351 339 337 297
252 182 174 154 115 104 97 66
61 36 35 27 11
EagI C'GGCC_G
Cuts at: 690 1076 3719 8615 690
Size: 386 2643 4896 3105
Fragments arranged by size:
4896 3105 2643 386
EarI CTCTTCn'nnn_
Cuts at: 3529 4685 7801 9053 9263 3529
Size: 1156 3116 1252 210 5296
Fragments arranged by size:
5296 3116 1252 1156 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 229 394 817 2059 9238 9980 10126 10840 229
Size: 165 423 1242 7179 742 146 714 419
Fragments arranged by size:
7179 1242 742 714 423 419 165 146
EcoICRI GAG'CTC
Cuts at: 8042 8042
Size: 11030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 529 654 934 1300 1429 1561 1672 1885
Size: 231 125 280 366 129 132 111 213
Cuts at: 1885 2367 2564 3637 6100 6394 6521 6847 6857
Size: 482 197 1073 2463 294 127 326 10
Cuts at: 6857 6863 6908 7228 8855 9287 9640 10302 298
Size: 6 45 320 1627 432 353 662 1026
Fragments arranged by size:
2463 1627 1073 1026 662 482 432 366
353 326 320 294 280 231 213 197
132 129 127 125 111 45 10 6
EcoNI CCTnn'n_nnAGG
Cuts at: 214 7942 214
Size: 7728 3302
EcoO109I rG'GnC_Cy
Cuts at: 207 696 1071 1270 1371 1572 1668 1669 1701
Size: 489 375 199 101 201 96 1 32
Cuts at: 1701 1854 1855 2220 2554 2742 2743 3136 3223
Size: 153 1 365 334 188 1 393 87
Cuts at: 3223 3224 3423 3424 3607 3788 4287 4288 4425
Size: 1 199 1 183 181 499 1 137
Cuts at: 4425 4573 4657 4658 4839 5397 5408 5450 5772
Size: 148 84 1 181 558 11 42 322
Cuts at: 5772 5793 6249 6339 6340 6369 6775 6776 6816
Size: 21 456 90 1 29 406 1 40
Cuts at: 6816 6817 6912 6913 7365 7636 7727 7728 7735
Size: 1 95 1 452 271 91 1 7
Cuts at: 7735 7773 7877 8169 207
Size: 38 104 292 3068
Fragments arranged by size:
3068 558 499 489 456 452 406 393
375 365 334 322 292 271 201 199
199 188 183 181 181 153 148 137
104 101 96 95 91 90 87 84
42 40 38 32 29 21 11 7
1 1 1 1 1 1 1 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 6856 8034 6856
Size: 1178 9852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1230 7830 9049 1230
Size: 6600 1219 3211
Fragments arranged by size:
6600 3211 1219
FspI TGC'GCA
Cuts at: 8811 8811
Size: 11030
HaeII r_GCGC'y
Cuts at: 439 707 1637 3392 8464 8712 9942 10713 439
Size: 268 930 1755 5072 248 1230 771 756
Fragments arranged by size:
5072 1755 1230 930 771 756 268 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 412 444 733 765 856 888 1149 1181 4848
Size: 32 289 32 91 32 261 32 3667
Cuts at: 4848 4880 5305 5337 5782 5814 7474 7506 7660
Size: 32 425 32 445 32 1660 32 154
Cuts at: 7660 7692 8872 8902 8904 8934 412
Size: 32 1180 30 2 30 2508
Fragments arranged by size:
3667 2508 1660 1180 445 425 289 261
154 91 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 171 2175 5673 5748 7125 8069 171
Size: 2004 3498 75 1377 944 3132
Fragments arranged by size:
3498 3132 2004 1377 944 75
KasI G'GCGC_C
Cuts at: 703 3388 8460 8708 703
Size: 2685 5072 248 3025
Fragments arranged by size:
5072 3025 2685 248
KpnI G_GTAC'C
Cuts at: 4699 8050 4699
Size: 3351 7679
MluI A'CGCG_T
Cuts at: 10463 10463
Size: 11030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 6188 9789 9973 329
Size: 5859 3601 184 1386
Fragments arranged by size:
5859 3601 1386 184
MscI TGG'CCA
Cuts at: 1043 1193 1532 2507 4000 4397 5681 5785 6098
Size: 150 339 975 1493 397 1284 104 313
Cuts at: 6098 6164 6175 6544 6898 7768 8791 1043
Size: 66 11 369 354 870 1023 3282
Fragments arranged by size:
3282 1493 1284 1023 975 870 397 369
354 339 313 150 104 66 11
MslI CAynn'nnrTG
Cuts at: 559 565 2341 2650 3746 4783 5251 5959 6190
Size: 6 1776 309 1096 1037 468 708 231
Cuts at: 6190 7966 9019 9146 559
Size: 1776 1053 127 2443
Fragments arranged by size:
2443 1776 1776 1096 1053 1037 708 468
309 231 127 6
MspA1I CmG'CkG
Cuts at: 393 654 719 1407 1436 1609 2303 3151 3631
Size: 261 65 688 29 173 694 848 480
Cuts at: 3631 4334 5352 5816 6697 7426 8141 8455 8815
Size: 703 1018 464 881 729 715 314 360
Cuts at: 8815 9599 9844 393
Size: 784 245 1579
Fragments arranged by size:
1579 1018 881 848 784 729 715 703
694 688 480 464 360 314 261 245
173 65 29
NaeI GCC'GGC
Cuts at: 194 1491 3393 7918 9211 194
Size: 1297 1902 4525 1293 2013
Fragments arranged by size:
4525 2013 1902 1297 1293
NarI GG'CG_CC
Cuts at: 704 3389 8461 8709 704
Size: 2685 5072 248 3025
Fragments arranged by size:
5072 3025 2685 248
NcoI C'CATG_G
Cuts at: 1376 1454 1739 2342 5712 7550 1376
Size: 78 285 603 3370 1838 4856
Fragments arranged by size:
4856 3370 1838 603 285 78
NgoMIV G'CCGG_C
Cuts at: 192 1489 3391 7916 9209 192
Size: 1297 1902 4525 1293 2013
Fragments arranged by size:
4525 2013 1902 1297 1293
NspI r_CATG'y
Cuts at: 3473 4056 5631 5843 6151 7851 8083 8583 10186
Size: 583 1575 212 308 1700 232 500 1603
Cuts at: 10186 3473
Size: 4317
Fragments arranged by size:
4317 1700 1603 1575 583 500 308 232
212
PasI CC'CwG_GG
Cuts at: 699 1858 5376 699
Size: 1159 3518 6353
Fragments arranged by size:
6353 3518 1159
PciI A'CATG_T
Cuts at: 3469 10182 3469
Size: 6713 4317
PflMI CCAn_nnn'nTGG
Cuts at: 1772 6171 1772
Size: 4399 6631
PfoI T'CCnGG_A
Cuts at: 446 1367 2240 3734 6596 6980 446
Size: 921 873 1494 2862 384 4496
Fragments arranged by size:
4496 2862 1494 921 873 384
PmeI GTTT'AAAC
Cuts at: 8029 8029
Size: 11030
PmlI CAC'GTG
Cuts at: 1772 2046 2140 3567 5274 6102 6171 6456 6744
Size: 274 94 1427 1707 828 69 285 288
Cuts at: 6744 7908 1772
Size: 1164 4894
Fragments arranged by size:
4894 1707 1427 1164 828 288 285 274
94 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9476 9508 9476
Size: 32 10998
PpuMI rG'GwC_Cy
Cuts at: 207 1071 1270 1371 1701 4425 6369 7365 7735
Size: 864 199 101 330 2724 1944 996 370
Cuts at: 7735 7877 207
Size: 142 3360
Fragments arranged by size:
3360 2724 1944 996 864 370 330 199
142 101
PshAI GACnn'nnGTC
Cuts at: 4118 4118
Size: 11030
PspOMI G'GGCC_C
Cuts at: 695 1668 1854 2742 3135 3223 3423 4287 4471
Size: 973 186 888 393 88 200 864 184
Cuts at: 4471 4573 4657 5396 5793 6339 6775 6816 6912
Size: 102 84 739 397 546 436 41 96
Cuts at: 6912 7727 695
Size: 815 3998
Fragments arranged by size:
3998 973 888 864 815 739 546 436
397 393 200 186 184 102 96 88
84 41
PspXI vC'TCGA_Gb
Cuts at: 439 3442 3663 439
Size: 3003 221 7806
Fragments arranged by size:
7806 3003 221
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6658 6690 6658
Size: 32 10998
PstI C_TGCA'G
Cuts at: 311 1441 3341 3851 6282 7831 8077 8762 311
Size: 1130 1900 510 2431 1549 246 685 2579
Fragments arranged by size:
2579 2431 1900 1549 1130 685 510 246
PvuI CG_AT'CG
Cuts at: 104 7175 104
Size: 7071 3959
PvuII CAG'CTG
Cuts at: 654 719 1407 1609 2303 4334 5352 5816 7426
Size: 65 688 202 694 2031 1018 464 1610
Cuts at: 7426 8455 8815 654
Size: 1029 360 2869
Fragments arranged by size:
2869 2031 1610 1029 1018 694 688 464
360 202 65
RsrII CG'GwC_CG
Cuts at: 9225 9225
Size: 11030
SacI G_AGCT'C
Cuts at: 8044 8044
Size: 11030
SalI G'TCGA_C
Cuts at: 169 8067 169
Size: 7898 3132
SanDI GG'GwC_CC
Cuts at: 7365 7365
Size: 11030
SapI GCTCTTCn'nnn_
Cuts at: 7801 9053 9263 7801
Size: 1252 210 9568
Fragments arranged by size:
9568 1252 210
SbfI CC_TGCA'GG
Cuts at: 8077 8077
Size: 11030
ScaI AGT'ACT
Cuts at: 4676 10547 4676
Size: 5871 5159
SexAI A'CCwGG_T
Cuts at: 6140 6140
Size: 11030
SfcI C'TryA_G
Cuts at: 33 307 1437 3337 3847 4280 4371 4662 5877
Size: 274 1130 1900 510 433 91 291 1215
Cuts at: 5877 6278 7827 7923 8073 8758 9726 9917 33
Size: 401 1549 96 150 685 968 191 1146
Fragments arranged by size:
1900 1549 1215 1146 1130 968 685 510
433 401 291 274 191 150 96 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 3997 3997
Size: 11030
SfoI GGC'GCC
Cuts at: 705 3390 8462 8710 705
Size: 2685 5072 248 3025
Fragments arranged by size:
5072 3025 2685 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11030
SmaI CCC'GGG
Cuts at: 728 1417 2629 3501 3612 3786 3955 4177 4767
Size: 689 1212 872 111 174 169 222 590
Cuts at: 4767 6874 7733 8052 728
Size: 2107 859 319 3706
Fragments arranged by size:
3706 2107 1212 872 859 689 590 319
222 174 169 111
SmlI C'TyrA_G
Cuts at: 418 439 2458 3442 3663 7942 8185 9100 9814
Size: 21 2019 984 221 4279 243 915 714
Cuts at: 9814 10076 418
Size: 262 1372
Fragments arranged by size:
4279 2019 1372 984 915 714 262 243
221 21
SnaBI TAC'GTA
Cuts at: 7797 7797
Size: 11030
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11030
SphI G_CATG'C
Cuts at: 8083 8583 8083
Size: 500 10530
SrfI GCCC'GGGC
Cuts at: 3955 3955
Size: 11030
StuI AGG'CCT
Cuts at: 3176 4583 6893 7792 3176
Size: 1407 2310 899 6414
Fragments arranged by size:
6414 2310 1407 899
StyI C'CwwG_G
Cuts at: 902 1266 1376 1454 1583 1673 1739 2342 2852
Size: 364 110 78 129 90 66 603 510
Cuts at: 2852 3383 4454 5712 5753 6122 6780 7163 7181
Size: 531 1071 1258 41 369 658 383 18
Cuts at: 7181 7493 7550 7700 7769 7873 8164 8518 902
Size: 312 57 150 69 104 291 354 3414
Fragments arranged by size:
3414 1258 1071 658 603 531 510 383
369 364 354 312 291 150 129 110
104 90 78 69 66 57 41 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2135 2800 3550 4171 4329 4639 5407 5598 5725
Size: 665 750 621 158 310 768 191 127
Cuts at: 5725 7324 8613 8746 9205 9414 2135
Size: 1599 1289 133 459 209 3751
Fragments arranged by size:
3751 1599 1289 768 750 665 621 459
310 209 191 158 133 127
TatI w'GTAC_w
Cuts at: 1622 2618 2915 3728 4055 4067 4346 4674 5363
Size: 996 297 813 327 12 279 328 689
Cuts at: 5363 5894 6728 7958 10545 1622
Size: 531 834 1230 2587 2107
Fragments arranged by size:
2587 2107 1230 996 834 813 689 531
328 327 297 279 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 2216 2248 3038 3070 6370 6402 10547
Size: 32 1973 32 790 32 3300 32 4145
Cuts at: 10547 10579 211
Size: 32 662
Fragments arranged by size:
4145 3300 1973 790 662 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5461 8827 5461
Size: 3366 7664
XbaI T'CTAG_A
Cuts at: 8061 8061
Size: 11030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 993 4584 4800 6211 6457 6745 7500 7761 8525
Size: 3591 216 1411 246 288 755 261 764
Cuts at: 8525 993
Size: 3498
Fragments arranged by size:
3591 3498 1411 764 755 288 261 246
216
XhoI C'TCGA_G
Cuts at: 439 3442 3663 439
Size: 3003 221 7806
Fragments arranged by size:
7806 3003 221
XmaI C'CCGG_G
Cuts at: 726 1415 2627 3499 3610 3784 3953 4175 4765
Size: 689 1212 872 111 174 169 222 590
Cuts at: 4765 6872 7731 8050 726
Size: 2107 859 319 3706
Fragments arranged by size:
3706 2107 1212 872 859 689 590 319
222 174 169 111
XmnI GAAnn'nnTTC
Cuts at: 250 7028 250
Size: 6778 4252
ZraI GAC'GTC
Cuts at: 2517 2832 7293 2517
Size: 315 4461 6254
Fragments arranged by size:
6254 4461 315
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI BaeI BfrBI BmtI
BsiWI BspEI BssHII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
SacII SgrAI SspI SwaI