Restriction map for
pF1KF0386
(Circular) (Six-base) MAPSORT of: pF1KF0386.seq Check: 8423 from: 1 to: 5135
pF1KF0386 5135 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 354 1077 354
Size: 723 4412
AccI GT'mk_AC
Cuts at: 904 2173 904
Size: 1269 3866
Acc65I G'GTAC_C
Cuts at: 2151 2151
Size: 5135
AclI AA'CG_TT
Cuts at: 4975 4975
Size: 5135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1251 1393 1531 2960 3392 3745 4407 268
Size: 983 142 138 1429 432 353 662 996
Fragments arranged by size:
1429 996 983 662 432 353 142 138
AflIII A'CryG_T
Cuts at: 2487 4287 4568 2487
Size: 1800 281 3054
Fragments arranged by size:
3054 1800 281
AleI CACnn'nnGTG
Cuts at: 1300 1300
Size: 5135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1813 1845 1813
Size: 32 5103
AlwNI CAG_nnn'CTG
Cuts at: 162 597 741 753 1362 2440 3878 162
Size: 435 144 12 609 1078 1438 1419
Fragments arranged by size:
1438 1419 1078 609 435 144 12
ApaI G_GGCC'C
Cuts at: 2097 2097
Size: 5135
ApaLI G'TGCA_C
Cuts at: 1743 2020 3973 1743
Size: 277 1953 2905
Fragments arranged by size:
2905 1953 277
ApoI r'AATT_y
Cuts at: 922 2139 4488 4744 922
Size: 1217 2349 256 1313
Fragments arranged by size:
2349 1313 1217 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5135
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
AvaI C'yCGr_G
Cuts at: 260 2155 260
Size: 1895 3240
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1594 1627 1594
Size: 33 5102
BamHI G'GATC_C
Cuts at: 2160 2160
Size: 5135
BanI G'GyrC_C
Cuts at: 1566 1590 2151 2565 2813 2848 1566
Size: 24 561 414 248 35 3853
Fragments arranged by size:
3853 561 414 248 35 24
BanII G_rGCy'C
Cuts at: 936 1240 1327 1400 1934 2097 2149 3179 936
Size: 304 87 73 534 163 52 1030 2892
Fragments arranged by size:
2892 1030 534 304 163 87 73 52
BbeI G_GCGC'C
Cuts at: 2569 2817 2569
Size: 248 4887
BbsI GAAGACnn'nnnn_
Cuts at: 1442 1519 1656 1442
Size: 77 137 4921
Fragments arranged by size:
4921 137 77
BbvCI CC'TCA_GC
Cuts at: 1346 1346
Size: 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 927 961 927
Size: 34 5101
BciVI GTATCCnnnnn_n'
Cuts at: 3033 4089 5135 3033
Size: 1056 1046 3033
Fragments arranged by size:
3033 1056 1046
BclI T'GATC_A
Cuts at: 1412 2655 1412
Size: 1243 3892
BfrBI ATG'CAT
Cuts at: 765 765
Size: 5135
BglI GCCn_nnn'nGGC
Cuts at: 1331 1642 1331
Size: 311 4824
BglII A'GATC_T
Cuts at: 2650 4687 2650
Size: 2037 3098
BlpI GC'TnA_GC
Cuts at: 461 2247 461
Size: 1786 3349
Bme1580I G_kGCm'C
Cuts at: 348 1571 1747 1943 2024 2097 2760 2853 3977
Size: 1223 176 196 81 73 663 93 1124
Cuts at: 3977 348
Size: 1506
Fragments arranged by size:
1506 1223 1124 663 196 176 93 81
73
BmrI ACTGGGnnnn_n'
Cuts at: 952 1190 1234 1729 1784 2347 2428 2763 4907
Size: 238 44 495 55 563 81 335 2144
Cuts at: 4907 952
Size: 1180
Fragments arranged by size:
2144 1180 563 495 335 238 81 55
44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3502 3502
Size: 5135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 502 794 1320 1561 1783 1948 1996 2022
Size: 159 292 526 241 222 165 48 26
Cuts at: 2022 343
Size: 3456
Fragments arranged by size:
3456 526 292 241 222 165 159 48
26
Bpu10I CC'TnA_GC
Cuts at: 1004 1346 1004
Size: 342 4793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1534 1783 2311 3190 3904 4202 314
Size: 1220 249 528 879 714 298 1247
Fragments arranged by size:
1247 1220 879 714 528 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 121 4839 121
Size: 4718 417
BsaAI yAC'GTr
Cuts at: 2488 2488
Size: 5135
BsaBI GATnn'nnATC
Cuts at: 6 2649 4686 6
Size: 2643 2037 455
Fragments arranged by size:
2643 2037 455
BsaHI Gr'CG_yC
Cuts at: 1997 2566 2814 3516 5012 1997
Size: 569 248 702 1496 2120
Fragments arranged by size:
2120 1496 702 569 248
BsaWI w'CCGG_w
Cuts at: 2324 2845 3523 3934 4081 4355 2324
Size: 521 678 411 147 274 3104
Fragments arranged by size:
3104 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1022 1052 1342 1372 1022
Size: 30 290 30 4785
Fragments arranged by size:
4785 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1363 1363
Size: 5135
BseYI C'CCAG_C
Cuts at: 347 683 746 800 857 1121 1147 1210 1309
Size: 336 63 54 57 264 26 63 99
Cuts at: 1309 1326 2560 3318 3983 4391 347
Size: 17 1234 758 665 408 1091
Fragments arranged by size:
1234 1091 758 665 408 336 264 99
63 63 57 54 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 967 982 994 1111 520
Size: 447 15 12 117 4544
Fragments arranged by size:
4544 447 117 15 12
BsiEI CG_ry'CG
Cuts at: 104 2677 2723 3953 104
Size: 2573 46 1230 1286
Fragments arranged by size:
2573 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 145 936 1747 1799 1934 2024 2149 2927 3117
Size: 791 811 52 135 90 125 778 190
Cuts at: 3117 3977 145
Size: 860 1303
Fragments arranged by size:
1303 860 811 791 778 190 135 125
90 52
Bsp1286I G_dGCh'C
Cuts at: 145 348 936 1240 1327 1400 1571 1747 1799
Size: 203 588 304 87 73 171 176 52
Cuts at: 1799 1934 1943 2024 2097 2149 2760 2853 2927
Size: 135 9 81 73 52 611 93 74
Cuts at: 2927 3117 3179 3977 145
Size: 190 62 798 1303
Fragments arranged by size:
1303 798 611 588 304 203 190 176
171 135 93 87 81 74 73 73
62 52 52 9
BspHI T'CATG_A
Cuts at: 1319 3567 1319
Size: 2248 2887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 354 1077 2185 2399 2701 3082 354
Size: 723 1108 214 302 381 2407
Fragments arranged by size:
2407 1108 723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1626 1655 1776 3427 3481 4965 5130 50
Size: 1576 29 121 1651 54 1484 165 55
Fragments arranged by size:
1651 1576 1484 165 121 55 54 29
BsrDI GCAATG_nn'
Cuts at: 3047 3047
Size: 5135
BsrFI r'CCGG_y
Cuts at: 1979 3133 3314 1979
Size: 1154 181 3800
Fragments arranged by size:
3800 1154 181
BssSI C'ACGA_G
Cuts at: 280 3406 4114 280
Size: 3126 708 1301
Fragments arranged by size:
3126 1301 708
BstAPI GCAn_nnn'nTGC
Cuts at: 675 762 1107 2512 675
Size: 87 345 1405 3298
Fragments arranged by size:
3298 1405 345 87
BstBI TT'CG_AA
Cuts at: 3496 3496
Size: 5135
BstEII G'GTnAC_C
Cuts at: 2070 2070
Size: 5135
BstXI CCAn_nnnn'nTGG
Cuts at: 1833 1833
Size: 5135
BstYI r'GATC_y
Cuts at: 1 2160 2627 2650 2985 3231 3537 3549 3635
Size: 2159 467 23 335 246 306 12 86
Cuts at: 3635 4687 1
Size: 1052 449
Fragments arranged by size:
2159 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 905 905
Size: 5135
BtgI C'CryG_G
Cuts at: 107 107
Size: 5135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3085 3266 3429 3085
Size: 181 163 4791
Fragments arranged by size:
4791 181 163
BtsI GCAGTG_nn'
Cuts at: 1854 1990 2378 1854
Size: 136 388 4611
Fragments arranged by size:
4611 388 136
DraI TTT'AAA
Cuts at: 1292 2134 1292
Size: 842 4293
DrdI GACnn_nn'nnGTC
Cuts at: 1101 2841 4185 1101
Size: 1740 1344 2051
Fragments arranged by size:
2051 1740 1344
EaeI y'GGCC_r
Cuts at: 284 1140 1643 1811 2720 2894 3285 3312 4657
Size: 856 503 168 909 174 391 27 1345
Cuts at: 4657 284
Size: 762
Fragments arranged by size:
1345 909 856 762 503 391 174 168
27
EagI C'GGCC_G
Cuts at: 2720 2720
Size: 5135
EarI CTCTTCn'nnn_
Cuts at: 1459 3158 3368 1459
Size: 1699 210 3226
Fragments arranged by size:
3226 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 153 716 3343 4085 4231 4945 153
Size: 563 2627 742 146 714 343
Fragments arranged by size:
2627 742 714 563 343 146
EcoICRI GAG'CTC
Cuts at: 934 1932 2147 934
Size: 998 215 3922
Fragments arranged by size:
3922 998 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 343 502 794 1251 1320 1393 1531 1561
Size: 75 159 292 457 69 73 138 30
Cuts at: 1561 1783 1948 1996 2022 2960 3392 3745 4407
Size: 222 165 48 26 938 432 353 662
Cuts at: 4407 268
Size: 996
Fragments arranged by size:
996 938 662 457 432 353 292 222
165 159 138 75 73 69 48 30
26
EcoO109I rG'GnC_Cy
Cuts at: 1449 1905 2274 1449
Size: 456 369 4310
Fragments arranged by size:
4310 456 369
EcoRI G'AATT_C
Cuts at: 2139 2139
Size: 5135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 5135
FspI TGC'GCA
Cuts at: 2916 2916
Size: 5135
HaeII r_GCGC'y
Cuts at: 373 424 2569 2817 4047 4818 373
Size: 51 2145 248 1230 771 690
Fragments arranged by size:
2145 1230 771 690 248 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1008 1022 1040 1054 1098 1130 2050 2082 2977
Size: 14 18 14 44 32 920 32 895
Cuts at: 2977 3007 3009 3039 1008
Size: 30 2 30 3104
Fragments arranged by size:
3104 920 895 44 32 32 30 30
18 14 14 2
HincII GTy'rAC
Cuts at: 2174 2174
Size: 5135
HindIII A'AGCT_T
Cuts at: 332 332
Size: 5135
KasI G'GCGC_C
Cuts at: 2565 2813 2565
Size: 248 4887
KpnI G_GTAC'C
Cuts at: 2155 2155
Size: 5135
MluI A'CGCG_T
Cuts at: 4568 4568
Size: 5135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 648 1190 3894 4078 648
Size: 542 2704 184 1705
Fragments arranged by size:
2704 1705 542 184
MscI TGG'CCA
Cuts at: 286 1645 1813 2896 286
Size: 1359 168 1083 2525
Fragments arranged by size:
2525 1359 1083 168
MslI CAynn'nnrTG
Cuts at: 1300 1564 3124 3251 1300
Size: 264 1560 127 3184
Fragments arranged by size:
3184 1560 264 127
MspA1I CmG'CkG
Cuts at: 351 597 615 753 1330 1380 1945 1968 2027
Size: 246 18 138 577 50 565 23 59
Cuts at: 2027 2246 2560 2920 3704 3949 351
Size: 219 314 360 784 245 1537
Fragments arranged by size:
1537 784 577 565 360 314 246 245
219 138 59 50 23 18
NaeI GCC'GGC
Cuts at: 3316 3316
Size: 5135
NarI GG'CG_CC
Cuts at: 2566 2814 2566
Size: 248 4887
NcoI C'CATG_G
Cuts at: 107 107
Size: 5135
NgoMIV G'CCGG_C
Cuts at: 3314 3314
Size: 5135
NsiI A_TGCA'T
Cuts at: 767 767
Size: 5135
NspI r_CATG'y
Cuts at: 2188 2688 4291 2188
Size: 500 1603 3032
Fragments arranged by size:
3032 1603 500
PciI A'CATG_T
Cuts at: 4287 4287
Size: 5135
PflMI CCAn_nnn'nTGG
Cuts at: 366 864 1020 366
Size: 498 156 4481
Fragments arranged by size:
4481 498 156
PfoI T'CCnGG_A
Cuts at: 1026 1628 2054 1026
Size: 602 426 4107
Fragments arranged by size:
4107 602 426
PmeI GTTT'AAAC
Cuts at: 2134 2134
Size: 5135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1297 1329 3581 3613 1297
Size: 32 2252 32 2819
Fragments arranged by size:
2819 2252 32 32
PpuMI rG'GwC_Cy
Cuts at: 1449 1905 1449
Size: 456 4679
PspOMI G'GGCC_C
Cuts at: 2093 2093
Size: 5135
PstI C_TGCA'G
Cuts at: 356 377 602 620 758 1861 2051 2182 2867
Size: 21 225 18 138 1103 190 131 685
Cuts at: 2867 356
Size: 2624
Fragments arranged by size:
2624 1103 685 225 190 138 131 21
18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5135
PvuII CAG'CTG
Cuts at: 351 597 615 753 1330 1380 2560 2920 351
Size: 246 18 138 577 50 1180 360 2566
Fragments arranged by size:
2566 1180 577 360 246 138 50 18
RsrII CG'GwC_CG
Cuts at: 3330 3330
Size: 5135
SacI G_AGCT'C
Cuts at: 936 1934 2149 936
Size: 998 215 3922
Fragments arranged by size:
3922 998 215
SalI G'TCGA_C
Cuts at: 2172 2172
Size: 5135
SapI GCTCTTCn'nnn_
Cuts at: 1459 3158 3368 1459
Size: 1699 210 3226
Fragments arranged by size:
3226 1699 210
SbfI CC_TGCA'GG
Cuts at: 2182 2182
Size: 5135
ScaI AGT'ACT
Cuts at: 4652 4652
Size: 5135
SfcI C'TryA_G
Cuts at: 33 352 373 424 598 616 754 1857 2047
Size: 319 21 51 174 18 138 1103 190
Cuts at: 2047 2178 2863 3831 4022 33
Size: 131 685 968 191 1146
Fragments arranged by size:
1146 1103 968 685 319 191 190 174
138 131 51 21 18
SfoI GGC'GCC
Cuts at: 2567 2815 2567
Size: 248 4887
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
SmaI CCC'GGG
Cuts at: 262 2157 262
Size: 1895 3240
SmlI C'TyrA_G
Cuts at: 329 1549 1762 2290 3205 3919 4181 329
Size: 1220 213 528 915 714 262 1283
Fragments arranged by size:
1283 1220 915 714 528 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5135
SphI G_CATG'C
Cuts at: 2188 2688 2188
Size: 500 4635
StuI AGG'CCT
Cuts at: 2113 2113
Size: 5135
StyI C'CwwG_G
Cuts at: 107 2269 2623 107
Size: 2162 354 2619
Fragments arranged by size:
2619 2162 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 1737 2058 2718 2851 3310 3519 145
Size: 1592 321 660 133 459 209 1761
Fragments arranged by size:
1761 1592 660 459 321 209 133
TatI w'GTAC_w
Cuts at: 993 1388 4650 993
Size: 395 3262 1478
Fragments arranged by size:
3262 1478 395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 199 215 231 1677 1709 4652 4684 183
Size: 16 16 16 1446 32 2943 32 634
Fragments arranged by size:
2943 1446 634 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 2932 2932
Size: 5135
XbaI T'CTAG_A
Cuts at: 2166 2166
Size: 5135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 225 2630 225
Size: 2405 2730
XmaI C'CCGG_G
Cuts at: 260 2155 260
Size: 1895 3240
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI AvrII
BmgBI BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI