Restriction map for
pF1KF0405
(Circular) (Six-base) MAPSORT of: pF1KF0405.seq Check: 5171 from: 1 to: 4319
pF1KF0405 4319 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 685 685
Size: 4319
AccI GT'mk_AC
Cuts at: 476 1357 476
Size: 881 3438
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 204 4159 204
Size: 3955 364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 432 668 748 2144 2576 2929 3591 395
Size: 37 236 80 1396 432 353 662 1123
Fragments arranged by size:
1396 1123 662 432 353 236 80 37
AflIII A'CryG_T
Cuts at: 1671 3471 3752 1671
Size: 1800 281 2238
Fragments arranged by size:
2238 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 726 1624 3062 726
Size: 898 1438 1983
Fragments arranged by size:
1983 1438 898
ApaLI G'TGCA_C
Cuts at: 484 3157 484
Size: 2673 1646
ApoI r'AATT_y
Cuts at: 828 1323 3672 3928 828
Size: 495 2349 256 1219
Fragments arranged by size:
2349 1219 495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 1339 1339
Size: 4319
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 1335 1749 1997 2032 1335
Size: 414 248 35 3622
Fragments arranged by size:
3622 414 248 35
BanII G_rGCy'C
Cuts at: 118 1333 2363 118
Size: 1215 1030 2074
Fragments arranged by size:
2074 1215 1030
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbsI GAAGACnn'nnnn_
Cuts at: 638 1109 638
Size: 471 3848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 444 478 444
Size: 34 4285
BciVI GTATCCnnnnn_n'
Cuts at: 2217 3273 4319 2217
Size: 1056 1046 2217
Fragments arranged by size:
2217 1056 1046
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BfrBI ATG'CAT
Cuts at: 298 1057 298
Size: 759 3560
BglI GCCn_nnn'nGGC
Cuts at: 907 907
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 488 1944 2037 3161 488
Size: 1456 93 1124 1646
Fragments arranged by size:
1646 1456 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1531 1612 1947 4091 1531
Size: 81 335 2144 1759
Fragments arranged by size:
2144 1759 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 236 2686 236
Size: 2450 1869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 592 585
Size: 7 4312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2374 3088 3386 1495
Size: 879 714 298 2428
Fragments arranged by size:
2428 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4023 4023
Size: 4319
BsaAI yAC'GTr
Cuts at: 837 906 1672 837
Size: 69 766 3484
Fragments arranged by size:
3484 766 69
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 1750 1998 2700 4196 1750
Size: 248 702 1496 1873
Fragments arranged by size:
1873 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1508 2029 2707 3118 3265 3539 1508
Size: 521 678 411 147 274 2288
Fragments arranged by size:
2288 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 852 882 952 982 852
Size: 30 70 30 4189
Fragments arranged by size:
4189 70 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 854 857 860 854
Size: 3 3 4313
Fragments arranged by size:
4313 3 3
BseYI C'CCAG_C
Cuts at: 385 1300 1744 2502 3167 3575 385
Size: 915 444 758 665 408 1129
Fragments arranged by size:
1129 915 758 665 444 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4319
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3137 104
Size: 1757 46 1230 1286
Fragments arranged by size:
1757 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 488 1333 2111 2301 3161 488
Size: 845 778 190 860 1646
Fragments arranged by size:
1646 860 845 778 190
BsmI GAATG_Cn'
Cuts at: 1246 1246
Size: 4319
BsmBI CGTCTCn'nnnn_
Cuts at: 149 149
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 118 488 1333 1944 2037 2111 2301 2363 3161
Size: 370 845 611 93 74 190 62 798
Cuts at: 3161 118
Size: 1276
Fragments arranged by size:
1276 845 798 611 370 190 93 74
62
BspHI T'CATG_A
Cuts at: 873 2751 873
Size: 1878 2441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 685 1369 1583 1885 2266 334
Size: 351 684 214 302 381 2387
Fragments arranged by size:
2387 684 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 2317 2498 2317
Size: 181 4138
BsrGI T'GTAC_A
Cuts at: 1134 1134
Size: 4319
BssSI C'ACGA_G
Cuts at: 2590 3298 2590
Size: 708 3611
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 1696
Size: 4319
BstBI TT'CG_AA
Cuts at: 345 2680 345
Size: 2335 1984
BstXI CCAn_nnnn'nTGG
Cuts at: 784 784
Size: 4319
BstYI r'GATC_y
Cuts at: 1 596 1344 1811 1834 2169 2415 2721 2733
Size: 595 748 467 23 335 246 306 12
Cuts at: 2733 2819 3871 1
Size: 86 1052 449
Fragments arranged by size:
1052 748 595 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1122 1122
Size: 4319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1243 2269 2450 2613 1243
Size: 1026 181 163 2949
Fragments arranged by size:
2949 1026 181 163
BtsI GCAGTG_nn'
Cuts at: 530 1562 530
Size: 1032 3287
DraI TTT'AAA
Cuts at: 516 738 1318 516
Size: 222 580 3517
Fragments arranged by size:
3517 580 222
DrdI GACnn_nn'nnGTC
Cuts at: 2025 3369 2025
Size: 1344 2975
EaeI y'GGCC_r
Cuts at: 1125 1904 2078 2469 2496 3841 1125
Size: 779 174 391 27 1345 1603
Fragments arranged by size:
1603 1345 779 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 374 451 2342 2552 374
Size: 77 1891 210 2141
Fragments arranged by size:
2141 1891 210 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 3269 3415 4129 2527
Size: 742 146 714 2717
Fragments arranged by size:
2717 742 714 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 432 585 592 668 748 2144 2576 2929
Size: 37 153 7 76 80 1396 432 353
Cuts at: 2929 3591 395
Size: 662 1123
Fragments arranged by size:
1396 1123 662 432 353 153 80 76
37 7
EcoO109I rG'GnC_Cy
Cuts at: 1458 1458
Size: 4319
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 4319
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 1753 2001 3231 4002 1753
Size: 248 1230 771 2070
Fragments arranged by size:
2070 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 952 984 2161 2191 2193 2223 952
Size: 32 1177 30 2 30 3048
Fragments arranged by size:
3048 1177 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 765 3078 3262 765
Size: 2313 184 1822
Fragments arranged by size:
2313 1822 184
MscI TGG'CCA
Cuts at: 2080 2080
Size: 4319
MslI CAynn'nnrTG
Cuts at: 2308 2435 2308
Size: 127 4192
MspA1I CmG'CkG
Cuts at: 542 1304 1430 1744 2104 2888 3133 542
Size: 762 126 314 360 784 245 1728
Fragments arranged by size:
1728 784 762 360 314 245 126
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NruI TCG'CGA
Cuts at: 1262 1262
Size: 4319
NsiI A_TGCA'T
Cuts at: 300 1059 300
Size: 759 3560
NspI r_CATG'y
Cuts at: 1372 1872 3475 1372
Size: 500 1603 2216
Fragments arranged by size:
2216 1603 500
PciI A'CATG_T
Cuts at: 3471 3471
Size: 4319
PfoI T'CCnGG_A
Cuts at: 1060 1060
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 373 405 2765 2797 373
Size: 32 2360 32 1895
Fragments arranged by size:
2360 1895 32 32
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 542 1304 1744 2104 542
Size: 762 440 360 2757
Fragments arranged by size:
2757 762 440 360
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 451 2342 2552 451
Size: 1891 210 2218
Fragments arranged by size:
2218 1891 210
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 987 1362 2047 3015 3206 33
Size: 954 375 685 968 191 1146
Fragments arranged by size:
1146 968 954 685 375 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 1474 2389 3103 3365 1474
Size: 915 714 262 2428
Fragments arranged by size:
2428 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StyI C'CwwG_G
Cuts at: 977 1453 1807 977
Size: 476 354 3489
Fragments arranged by size:
3489 476 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1902 2035 2494 2703 999
Size: 903 133 459 209 2615
Fragments arranged by size:
2615 903 459 209 133
TatI w'GTAC_w
Cuts at: 335 1134 3834 335
Size: 799 2700 820
Fragments arranged by size:
2700 820 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 814 846 1017 1049 3836 3868 814
Size: 32 171 32 2787 32 1265
Fragments arranged by size:
2787 1265 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1058 1135 1814 1058
Size: 77 679 3563
Fragments arranged by size:
3563 679 77
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
XmnI GAAnn'nnTTC
Cuts at: 716 716
Size: 4319
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I
BsiWI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI