Restriction map for
pF1KF0414
(Circular) (Six-base) MAPSORT of: pF1KF0414.seq Check: 22 from: 1 to: 6287
pF1KF0414 6287 bp
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 441 1587 3180 441
Size: 1146 1593 3548
Fragments arranged by size:
3548 1593 1146
AatII G_ACGT'C
Cuts at: 845 845
Size: 6287
AccI GT'mk_AC
Cuts at: 3325 3325
Size: 6287
Acc65I G'GTAC_C
Cuts at: 3303 3303
Size: 6287
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1337 1438 1593 2196 4112 4544 4897 5559
Size: 1147 101 155 603 1916 432 353 662
Cuts at: 5559 190
Size: 918
Fragments arranged by size:
1916 1147 918 662 603 432 353 155
101
AfeI AGC'GCT
Cuts at: 442 442
Size: 6287
AflIII A'CryG_T
Cuts at: 719 2238 2240 3639 5439 5720 719
Size: 1519 2 1399 1800 281 1286
Fragments arranged by size:
1800 1519 1399 1286 281 2
AhdI GACnn_n'nnGTC
Cuts at: 1329 1329
Size: 6287
AleI CACnn'nnGTG
Cuts at: 917 917
Size: 6287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2239 2239
Size: 6287
AlwNI CAG_nnn'CTG
Cuts at: 168 240 642 951 1635 2322 3592 5030 168
Size: 72 402 309 684 687 1270 1438 1425
Fragments arranged by size:
1438 1425 1270 687 684 402 309 72
ApaI G_GGCC'C
Cuts at: 782 1524 2552 2588 2823 2844 3033 782
Size: 742 1028 36 235 21 189 4036
Fragments arranged by size:
4036 1028 742 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 619 799 2122 5125 619
Size: 180 1323 3003 1781
Fragments arranged by size:
3003 1781 1323 180
ApoI r'AATT_y
Cuts at: 3291 5640 5896 3291
Size: 2349 256 3682
Fragments arranged by size:
3682 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 481 1464 1517 1906 2545 2603 2677 2775 2816
Size: 983 53 389 639 58 74 98 41
Cuts at: 2816 2822 2874 2973 3307 481
Size: 6 52 99 334 3461
Fragments arranged by size:
3461 983 639 389 334 99 98 74
58 53 52 41 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2227 2260 2227
Size: 33 6254
BamHI G'GATC_C
Cuts at: 2420 2722 3312 2420
Size: 302 590 5395
Fragments arranged by size:
5395 590 302
BanI G'GyrC_C
Cuts at: 2538 2613 3010 3303 3717 3965 4000 2538
Size: 75 397 293 414 248 35 4825
Fragments arranged by size:
4825 414 397 293 248 75 35
BanII G_rGCy'C
Cuts at: 583 782 794 1524 2036 2552 2588 2641 2823
Size: 199 12 730 512 516 36 53 182
Cuts at: 2823 2844 3033 3301 4331 583
Size: 21 189 268 1030 2539
Fragments arranged by size:
2539 1030 730 516 512 268 199 189
182 53 36 21 12
BbeI G_GCGC'C
Cuts at: 2617 3721 3969 2617
Size: 1104 248 4935
Fragments arranged by size:
4935 1104 248
BbsI GAAGACnn'nnnn_
Cuts at: 1507 1507
Size: 6287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1029 1063 1029
Size: 34 6253
BciVI GTATCCnnnnn_n'
Cuts at: 3239 4185 5241 6287 3239
Size: 946 1056 1046 3239
Fragments arranged by size:
3239 1056 1046 946
BclI T'GATC_A
Cuts at: 3807 3807
Size: 6287
BfrBI ATG'CAT
Cuts at: 1165 2705 1165
Size: 1540 4747
BglI GCCn_nnn'nGGC
Cuts at: 2333 2474 2547 2333
Size: 141 73 6073
Fragments arranged by size:
6073 141 73
BglII A'GATC_T
Cuts at: 3802 5839 3802
Size: 2037 4250
BlpI GC'TnA_GC
Cuts at: 643 3399 643
Size: 2756 3531
Bme1580I G_kGCm'C
Cuts at: 623 782 803 1524 2126 2543 2552 2588 2823
Size: 159 21 721 602 417 9 36 235
Cuts at: 2823 2844 3033 3912 4005 5129 623
Size: 21 189 879 93 1124 1781
Fragments arranged by size:
1781 1124 879 721 602 417 235 189
159 93 36 21 21 9
BmgBI CAC'GTC
Cuts at: 1326 1326
Size: 6287
BmrI ACTGGGnnnn_n'
Cuts at: 699 883 939 2845 3274 3499 3580 3915 6059
Size: 184 56 1906 429 225 81 335 2144
Cuts at: 6059 699
Size: 927
Fragments arranged by size:
2144 1906 927 429 335 225 184 81
56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4654 4654
Size: 6287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1374 1701 2934 2940 3091 1374
Size: 327 1233 6 151 4570
Fragments arranged by size:
4570 1233 327 151 6
Bpu10I CC'TnA_GC
Cuts at: 214 605 214
Size: 391 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1834 1900 3463 4342 5056 5354 1018
Size: 816 66 1563 879 714 298 1951
Fragments arranged by size:
1951 1563 879 816 714 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 1609 2098 5991 1609
Size: 489 3893 1905
Fragments arranged by size:
3893 1905 489
BsaAI yAC'GTr
Cuts at: 1845 2241 3640 1845
Size: 396 1399 4492
Fragments arranged by size:
4492 1399 396
BsaBI GATnn'nnATC
Cuts at: 6 3801 5838 6
Size: 3795 2037 455
Fragments arranged by size:
3795 2037 455
BsaHI Gr'CG_yC
Cuts at: 842 1585 2614 3718 3966 4668 6164 842
Size: 743 1029 1104 248 702 1496 965
Fragments arranged by size:
1496 1104 1029 965 743 702 248
BsaWI w'CCGG_w
Cuts at: 1934 2562 3476 3997 4675 5086 5233 5507 1934
Size: 628 914 521 678 411 147 274 2714
Fragments arranged by size:
2714 914 678 628 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1249 1279 1570 1600 1684 1714 2497 2527 2907
Size: 30 291 30 84 30 783 30 380
Cuts at: 2907 2937 1249
Size: 30 4599
Fragments arranged by size:
4599 783 380 291 84 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1141 1453 1689 2116 3088 1141
Size: 312 236 427 972 4340
Fragments arranged by size:
4340 972 427 312 236
BseYI C'CCAG_C
Cuts at: 324 546 770 1068 1980 2383 2571 2686 2770
Size: 222 224 298 912 403 188 115 84
Cuts at: 2770 3157 3712 4470 5135 5543 324
Size: 387 555 758 665 408 1068
Fragments arranged by size:
1068 912 758 665 555 408 403 387
298 224 222 188 115 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 226
Size: 6287
BsiEI CG_ry'CG
Cuts at: 104 3829 3875 5105 104
Size: 3725 46 1230 1286
Fragments arranged by size:
3725 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 611 623 803 1934 2126 3212 3218 3301 4079
Size: 12 180 1131 192 1086 6 83 778
Cuts at: 4079 4269 5129 611
Size: 190 860 1769
Fragments arranged by size:
1769 1131 1086 860 778 192 190 180
83 12 6
BsmBI CGTCTCn'nnnn_
Cuts at: 1275 1275
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 583 611 623 782 794 803 1524 1934 2036
Size: 28 12 159 12 9 721 410 102
Cuts at: 2036 2126 2543 2552 2588 2641 2823 2844 3033
Size: 90 417 9 36 53 182 21 189
Cuts at: 3033 3212 3218 3301 3912 4005 4079 4269 4331
Size: 179 6 83 611 93 74 190 62
Cuts at: 4331 5129 583
Size: 798 1741
Fragments arranged by size:
1741 798 721 611 417 410 190 189
182 179 159 102 93 90 83 74
62 53 36 28 21 12 12 9
9 6
BspEI T'CCGG_A
Cuts at: 1934 2562 1934
Size: 628 5659
BspHI T'CATG_A
Cuts at: 4719 4719
Size: 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 269 392 441 1587 2307 2565 3180 3337 3551
Size: 123 49 1146 720 258 615 157 214
Cuts at: 3551 3853 4234 269
Size: 302 381 2322
Fragments arranged by size:
2322 1146 720 615 381 302 258 214
157 123 49
BsrBI CCG'CTC
Cuts at: 50 2763 4579 4633 6117 6282 50
Size: 2713 1816 54 1484 165 55
Fragments arranged by size:
2713 1816 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1368 4199 1368
Size: 2831 3456
BsrFI r'CCGG_y
Cuts at: 112 1647 1750 2731 3106 4285 4466 112
Size: 1535 103 981 375 1179 181 1933
Fragments arranged by size:
1933 1535 1179 981 375 181 103
BssSI C'ACGA_G
Cuts at: 682 1048 3203 3211 3217 4558 5266 682
Size: 366 2155 8 6 1341 708 1703
Fragments arranged by size:
2155 1703 1341 708 366 8 6
BstAPI GCAn_nnn'nTGC
Cuts at: 240 1929 3664 240
Size: 1689 1735 2863
Fragments arranged by size:
2863 1735 1689
BstBI TT'CG_AA
Cuts at: 4648 4648
Size: 6287
BstEII G'GTnAC_C
Cuts at: 688 3167 688
Size: 2479 3808
BstXI CCAn_nnnn'nTGG
Cuts at: 1554 2523 1554
Size: 969 5318
BstYI r'GATC_y
Cuts at: 1 827 1043 2420 2722 3312 3779 3802 4137
Size: 826 216 1377 302 590 467 23 335
Cuts at: 4137 4383 4689 4701 4787 5839 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1377 1052 826 590 467 449 335 306
302 246 216 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3005 3005
Size: 6287
BtgI C'CryG_G
Cuts at: 107 804 1553 1989 3232 3246 107
Size: 697 749 436 1243 14 3148
Fragments arranged by size:
3148 1243 749 697 436 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 912 924 1002 1193 3530 912
Size: 12 78 191 2337 3669
Fragments arranged by size:
3669 2337 191 78 12
DraI TTT'AAA
Cuts at: 3286 3286
Size: 6287
DraIII CAC_nnn'GTG
Cuts at: 618 618
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 3993 5337 3993
Size: 1344 4943
EaeI y'GGCC_r
Cuts at: 110 1365 1427 1556 1947 2193 2435 2693 2764
Size: 1255 62 129 391 246 242 258 71
Cuts at: 2764 3872 4046 4437 4464 5809 110
Size: 1108 174 391 27 1345 588
Fragments arranged by size:
1345 1255 1108 588 391 391 258 246
242 174 129 71 62 27
EagI C'GGCC_G
Cuts at: 3872 3872
Size: 6287
EarI CTCTTCn'nnn_
Cuts at: 329 838 1313 2255 2344 2629 4310 4520 329
Size: 509 475 942 89 285 1681 210 2096
Fragments arranged by size:
2096 1681 942 509 475 285 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1806 3093 4495 5237 5383 6097 1806
Size: 1287 1402 742 146 714 1996
Fragments arranged by size:
1996 1402 1287 742 714 146
EcoICRI GAG'CTC
Cuts at: 3299 3299
Size: 6287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1337 1374 1438 1593 1701 2196 2934 2940
Size: 1147 37 64 155 108 495 738 6
Cuts at: 2940 3091 4112 4544 4897 5559 190
Size: 151 1021 432 353 662 918
Fragments arranged by size:
1147 1021 918 738 662 495 432 353
155 151 108 64 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1051 1051
Size: 6287
EcoO109I rG'GnC_Cy
Cuts at: 228 375 732 1251 1521 1896 2584 2748 2779
Size: 147 357 519 270 375 688 164 31
Cuts at: 2779 2840 2841 2892 2902 2909 2990 3029 3030
Size: 61 1 51 10 7 81 39 1
Cuts at: 3030 3426 228
Size: 396 3089
Fragments arranged by size:
3089 688 519 396 375 357 270 164
147 81 61 51 39 31 10 7
1 1
EcoRI G'AATT_C
Cuts at: 3291 3291
Size: 6287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 849 3135 4306 849
Size: 2286 1171 2830
Fragments arranged by size:
2830 2286 1171
FspI TGC'GCA
Cuts at: 4068 4068
Size: 6287
HaeII r_GCGC'y
Cuts at: 444 1826 2444 2617 3721 3969 5199 5970 444
Size: 1382 618 173 1104 248 1230 771 761
Fragments arranged by size:
1382 1230 1104 771 761 618 248 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 1315 1347 1570 1602 2497 2529 2907
Size: 32 313 32 223 32 895 32 378
Cuts at: 2907 2939 4129 4159 4161 4191 970
Size: 32 1190 30 2 30 3066
Fragments arranged by size:
3066 1190 895 378 313 223 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3326 3326
Size: 6287
HindIII A'AGCT_T
Cuts at: 1383 1383
Size: 6287
KasI G'GCGC_C
Cuts at: 2613 3717 3965 2613
Size: 1104 248 4935
Fragments arranged by size:
4935 1104 248
KpnI G_GTAC'C
Cuts at: 3307 3307
Size: 6287
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5046 5230 5046
Size: 184 6103
MscI TGG'CCA
Cuts at: 1558 1949 2195 4048 1558
Size: 391 246 1853 3797
Fragments arranged by size:
3797 1853 391 246
MslI CAynn'nnrTG
Cuts at: 422 614 917 1573 2275 2704 4276 4403 422
Size: 192 303 656 702 429 1572 127 2306
Fragments arranged by size:
2306 1572 702 656 429 303 192 127
MspA1I CmG'CkG
Cuts at: 237 642 752 1592 1803 1926 2303 2418 2594
Size: 405 110 840 211 123 377 115 176
Cuts at: 2594 2620 2999 3398 3712 4072 4856 5101 237
Size: 26 379 399 314 360 784 245 1423
Fragments arranged by size:
1423 840 784 405 399 379 377 360
314 245 211 176 123 115 110 26
NaeI GCC'GGC
Cuts at: 114 1649 1752 2733 4468 114
Size: 1535 103 981 1735 1933
Fragments arranged by size:
1933 1735 1535 981 103
NarI GG'CG_CC
Cuts at: 2614 3718 3966 2614
Size: 1104 248 4935
Fragments arranged by size:
4935 1104 248
NcoI C'CATG_G
Cuts at: 107 804 107
Size: 697 5590
NgoMIV G'CCGG_C
Cuts at: 112 1647 1750 2731 4466 112
Size: 1535 103 981 1735 1933
Fragments arranged by size:
1933 1735 1535 981 103
NsiI A_TGCA'T
Cuts at: 1167 2707 1167
Size: 1540 4747
NspI r_CATG'y
Cuts at: 723 1866 2130 2284 2705 3340 3840 5443 723
Size: 1143 264 154 421 635 500 1603 1567
Fragments arranged by size:
1603 1567 1143 635 500 421 264 154
PciI A'CATG_T
Cuts at: 719 5439 719
Size: 4720 1567
PfoI T'CCnGG_A
Cuts at: 2480 2666 2480
Size: 186 6101
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PmlI CAC'GTG
Cuts at: 2241 2241
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 4733
Size: 32 6255
PpuMI rG'GwC_Cy
Cuts at: 228 375 732 1251 1896 2902 2990 228
Size: 147 357 519 645 1006 88 3525
Fragments arranged by size:
3525 1006 645 519 357 147 88
PspOMI G'GGCC_C
Cuts at: 778 1520 2548 2584 2819 2840 3029 778
Size: 742 1028 36 235 21 189 4036
Fragments arranged by size:
4036 1028 742 235 189 36 21
PstI C_TGCA'G
Cuts at: 236 440 452 740 919 1640 1802 2050 2756
Size: 204 12 288 179 721 162 248 706
Cuts at: 2756 3019 3038 3334 4019 236
Size: 263 19 296 685 2504
Fragments arranged by size:
2504 721 706 685 296 288 263 248
204 179 162 19 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 237 1592 1803 1926 2620 3712 4072 237
Size: 1355 211 123 694 1092 360 2452
Fragments arranged by size:
2452 1355 1092 694 360 211 123
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 3301 3301
Size: 6287
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 2629 4310 4520 2629
Size: 1681 210 4396
Fragments arranged by size:
4396 1681 210
SbfI CC_TGCA'GG
Cuts at: 452 740 3019 3038 3334 452
Size: 288 2279 19 296 3405
Fragments arranged by size:
3405 2279 296 288 19
ScaI AGT'ACT
Cuts at: 5804 5804
Size: 6287
SexAI A'CCwGG_T
Cuts at: 1532 1532
Size: 6287
SfcI C'TryA_G
Cuts at: 33 232 399 436 448 736 915 1636 1798
Size: 199 167 37 12 288 179 721 162
Cuts at: 1798 2046 2232 2752 3015 3034 3330 4015 4983
Size: 248 186 520 263 19 296 685 968
Cuts at: 4983 5174 33
Size: 191 1146
Fragments arranged by size:
1146 968 721 685 520 296 288 263
248 199 191 186 179 167 162 37
19 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2333 2333
Size: 6287
SfoI GGC'GCC
Cuts at: 2615 3719 3967 2615
Size: 1104 248 4935
Fragments arranged by size:
4935 1104 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SgrAI Cr'CCGG_yG
Cuts at: 2731 2731
Size: 6287
SmaI CCC'GGG
Cuts at: 1466 1519 1908 2547 2679 2777 2818 2824 2876
Size: 53 389 639 132 98 41 6 52
Cuts at: 2876 2975 3309 1466
Size: 99 334 4444
Fragments arranged by size:
4444 639 389 334 132 99 98 53
52 41 6
SmlI C'TyrA_G
Cuts at: 481 1033 1849 1915 3442 4357 5071 5333 481
Size: 552 816 66 1527 915 714 262 1435
Fragments arranged by size:
1527 1435 915 816 714 552 262 66
SnaBI TAC'GTA
Cuts at: 1845 1845
Size: 6287
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
SrfI GCCC'GGGC
Cuts at: 1908 2824 2975 1908
Size: 916 151 5220
Fragments arranged by size:
5220 916 151
StuI AGG'CCT
Cuts at: 2266 2336 2266
Size: 70 6217
StyI C'CwwG_G
Cuts at: 107 315 804 963 1619 2516 2897 3262 3421
Size: 208 489 159 656 897 381 365 159
Cuts at: 3421 3775 107
Size: 354 2619
Fragments arranged by size:
2619 897 656 489 381 365 354 208
159 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 1576 1847 2784 3870 4003 4462 4671 676
Size: 900 271 937 1086 133 459 209 2292
Fragments arranged by size:
2292 1086 937 900 459 271 209 133
TatI w'GTAC_w
Cuts at: 1541 5802 1541
Size: 4261 2026
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1929 1961 5804 5836 1929
Size: 32 3843 32 2380
Fragments arranged by size:
3843 2380 32 32
Tth111I GACn'n_nGTC
Cuts at: 3195 4084 3195
Size: 889 5398
XbaI T'CTAG_A
Cuts at: 3318 3318
Size: 6287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 3782 2173
Size: 1609 4678
XhoI C'TCGA_G
Cuts at: 481 481
Size: 6287
XmaI C'CCGG_G
Cuts at: 1464 1517 1906 2545 2677 2775 2816 2822 2874
Size: 53 389 639 132 98 41 6 52
Cuts at: 2874 2973 3307 1464
Size: 99 334 4444
Fragments arranged by size:
4444 639 389 334 132 99 98 53
52 41 6
ZraI GAC'GTC
Cuts at: 843 843
Size: 6287
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BbvCI BmtI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PshAI PsiI PspXI PsrI SacII SanDI
SspI SwaI XmnI