Restriction map for
pF1KF0417
(Circular) (Six-base) MAPSORT of: pF1KF0417.seq Check: 8206 from: 1 to: 4730
pF1KF0417 4730 bp
With 182 enzymes: SgfI *
September 25, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 176 1623 176
Size: 1447 3283
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 2555 2987 3340 4002 639
Size: 1916 432 353 662 1367
Fragments arranged by size:
1916 1367 662 432 353
AfeI AGC'GCT
Cuts at: 427 427
Size: 4730
AflIII A'CryG_T
Cuts at: 681 683 2082 3882 4163 681
Size: 2 1399 1800 281 1248
Fragments arranged by size:
1800 1399 1248 281 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4730
AlwNI CAG_nnn'CTG
Cuts at: 765 2035 3473 765
Size: 1270 1438 2022
Fragments arranged by size:
2022 1438 1270
ApaI G_GGCC'C
Cuts at: 995 1031 1266 1287 1476 995
Size: 36 235 21 189 4249
Fragments arranged by size:
4249 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 565 3568 565
Size: 3003 1727
ApoI r'AATT_y
Cuts at: 1734 4083 4339 1734
Size: 2349 256 2125
Fragments arranged by size:
2349 2125 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 988 1046 1120 1218 1259 1265 1317 1416 1750
Size: 58 74 98 41 6 52 99 334
Cuts at: 1750 988
Size: 3968
Fragments arranged by size:
3968 334 99 98 74 58 52 41
6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 670 703 670
Size: 33 4697
BamHI G'GATC_C
Cuts at: 174 863 1165 1755 174
Size: 689 302 590 3149
Fragments arranged by size:
3149 689 590 302
BanI G'GyrC_C
Cuts at: 238 981 1056 1453 1746 2160 2408 2443 238
Size: 743 75 397 293 414 248 35 2525
Fragments arranged by size:
2525 743 414 397 293 248 75 35
BanII G_rGCy'C
Cuts at: 479 995 1031 1084 1266 1287 1476 1744 2774
Size: 516 36 53 182 21 189 268 1030
Cuts at: 2774 479
Size: 2435
Fragments arranged by size:
2435 1030 516 268 189 182 53 36
21
BbeI G_GCGC'C
Cuts at: 1060 2164 2412 1060
Size: 1104 248 3378
Fragments arranged by size:
3378 1104 248
BbsI GAAGACnn'nnnn_
Cuts at: 411 411
Size: 4730
BciVI GTATCCnnnnn_n'
Cuts at: 1682 2628 3684 4730 1682
Size: 946 1056 1046 1682
Fragments arranged by size:
1682 1056 1046 946
BclI T'GATC_A
Cuts at: 2250 2250
Size: 4730
BfrBI ATG'CAT
Cuts at: 1148 1148
Size: 4730
BglI GCCn_nnn'nGGC
Cuts at: 112 776 917 990 112
Size: 664 141 73 3852
Fragments arranged by size:
3852 664 141 73
BglII A'GATC_T
Cuts at: 368 2245 4282 368
Size: 1877 2037 816
Fragments arranged by size:
2037 1877 816
BlpI GC'TnA_GC
Cuts at: 1842 1842
Size: 4730
Bme1580I G_kGCm'C
Cuts at: 569 986 995 1031 1266 1287 1476 2355 2448
Size: 417 9 36 235 21 189 879 93
Cuts at: 2448 3572 569
Size: 1124 1727
Fragments arranged by size:
1727 1124 879 417 235 189 93 36
21 9
BmrI ACTGGGnnnn_n'
Cuts at: 1288 1717 1942 2023 2358 4502 1288
Size: 429 225 81 335 2144 1516
Fragments arranged by size:
2144 1516 429 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 3097 339
Size: 2758 1972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 1377 1383 1534 357
Size: 1020 6 151 3553
Fragments arranged by size:
3553 1020 151 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1906 2785 3499 3797 219
Size: 1687 879 714 298 1152
Fragments arranged by size:
1687 1152 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 541 4434 541
Size: 3893 837
BsaAI yAC'GTr
Cuts at: 684 2083 684
Size: 1399 3331
BsaBI GATnn'nnATC
Cuts at: 6 373 2244 4281 6
Size: 367 1871 2037 455
Fragments arranged by size:
2037 1871 455 367
BsaHI Gr'CG_yC
Cuts at: 1057 2161 2409 3111 4607 1057
Size: 1104 248 702 1496 1180
Fragments arranged by size:
1496 1180 1104 702 248
BsaWI w'CCGG_w
Cuts at: 177 1005 1919 2440 3118 3529 3676 3950 177
Size: 828 914 521 678 411 147 274 957
Fragments arranged by size:
957 914 828 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 940 970 1350 1380 172
Size: 30 738 30 380 30 3522
Fragments arranged by size:
3522 738 380 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 1531 559
Size: 972 3758
BseYI C'CCAG_C
Cuts at: 144 826 1014 1129 1213 1600 2155 2913 3578
Size: 682 188 115 84 387 555 758 665
Cuts at: 3578 3986 144
Size: 408 888
Fragments arranged by size:
888 758 682 665 555 408 387 188
115 84
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3548 104
Size: 2168 46 1230 1286
Fragments arranged by size:
2168 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 320 569 1655 1661 1744 2522 2712 3572 320
Size: 249 1086 6 83 778 190 860 1478
Fragments arranged by size:
1478 1086 860 778 249 190 83 6
Bsp1286I G_dGCh'C
Cuts at: 320 479 569 986 995 1031 1084 1266 1287
Size: 159 90 417 9 36 53 182 21
Cuts at: 1287 1476 1655 1661 1744 2355 2448 2522 2712
Size: 189 179 6 83 611 93 74 190
Cuts at: 2712 2774 3572 320
Size: 62 798 1478
Fragments arranged by size:
1478 798 611 417 190 189 182 179
159 93 90 83 74 62 53 36
21 9 6
BspEI T'CCGG_A
Cuts at: 177 1005 177
Size: 828 3902
BspHI T'CATG_A
Cuts at: 3162 3162
Size: 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 1008 1623 1780 1994 2296 2677 176
Size: 832 615 157 214 302 381 2229
Fragments arranged by size:
2229 832 615 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 228 323 1206 3022 3076 4560 4725 50
Size: 178 95 883 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 883 178 165 95 55 54
BsrDI GCAATG_nn'
Cuts at: 2642 2642
Size: 4730
BsrFI r'CCGG_y
Cuts at: 127 1174 1549 2728 2909 127
Size: 1047 375 1179 181 1948
Fragments arranged by size:
1948 1179 1047 375 181
BssSI C'ACGA_G
Cuts at: 1646 1654 1660 3001 3709 1646
Size: 8 6 1341 708 2667
Fragments arranged by size:
2667 1341 708 8 6
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2107
Size: 4730
BstBI TT'CG_AA
Cuts at: 3091 3091
Size: 4730
BstEII G'GTnAC_C
Cuts at: 1610 1610
Size: 4730
BstXI CCAn_nnnn'nTGG
Cuts at: 291 966 291
Size: 675 4055
BstYI r'GATC_y
Cuts at: 1 174 368 863 1165 1755 2222 2245 2580
Size: 173 194 495 302 590 467 23 335
Cuts at: 2580 2826 3132 3144 3230 4282 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 590 495 467 449 335 306 302
246 194 173 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 158 1448 158
Size: 1290 3440
BtgI C'CryG_G
Cuts at: 218 1675 1689 218
Size: 1457 14 3259
Fragments arranged by size:
3259 1457 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2680 2861 3024 2680
Size: 181 163 4386
Fragments arranged by size:
4386 181 163
BtsI GCAGTG_nn'
Cuts at: 1973 1973
Size: 4730
DraI TTT'AAA
Cuts at: 1729 1729
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3780 2436
Size: 1344 3386
EaeI y'GGCC_r
Cuts at: 636 878 1136 1207 2315 2489 2880 2907 4252
Size: 242 258 71 1108 174 391 27 1345
Cuts at: 4252 636
Size: 1114
Fragments arranged by size:
1345 1114 1108 391 258 242 174 71
27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 155 698 787 1072 2753 2963 155
Size: 543 89 285 1681 210 1922
Fragments arranged by size:
1922 1681 543 285 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 1536 2938 3680 3826 4540 143
Size: 1393 1402 742 146 714 333
Fragments arranged by size:
1402 1393 742 714 333 146
EcoICRI GAG'CTC
Cuts at: 1742 1742
Size: 4730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 639 1377 1383 1534 2555 2987 3340 4002
Size: 282 738 6 151 1021 432 353 662
Cuts at: 4002 357
Size: 1085
Fragments arranged by size:
1085 1021 738 662 432 353 282 151
6
EcoO109I rG'GnC_Cy
Cuts at: 162 288 341 1027 1191 1222 1283 1284 1335
Size: 126 53 686 164 31 61 1 51
Cuts at: 1335 1345 1352 1433 1472 1473 1869 162
Size: 10 7 81 39 1 396 3023
Fragments arranged by size:
3023 686 396 164 126 81 61 53
51 39 31 10 7 1 1
EcoRI G'AATT_C
Cuts at: 1734 1734
Size: 4730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1578 2749 1578
Size: 1171 3559
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 206 429 887 1060 2164 2412 3642 4413 206
Size: 223 458 173 1104 248 1230 771 523
Fragments arranged by size:
1230 1104 771 523 458 248 223 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 1350 1382 2572 2602 2604 2634 940
Size: 32 378 32 1190 30 2 30 3036
Fragments arranged by size:
3036 1190 378 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
KasI G'GCGC_C
Cuts at: 1056 2160 2408 1056
Size: 1104 248 3378
Fragments arranged by size:
3378 1104 248
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 392 3489 3673 392
Size: 3097 184 1449
Fragments arranged by size:
3097 1449 184
MscI TGG'CCA
Cuts at: 638 2491 638
Size: 1853 2877
MslI CAynn'nnrTG
Cuts at: 718 1147 2719 2846 718
Size: 429 1572 127 2602
Fragments arranged by size:
2602 1572 429 127
MspA1I CmG'CkG
Cuts at: 335 746 861 1037 1063 1442 1841 2155 2515
Size: 411 115 176 26 379 399 314 360
Cuts at: 2515 3299 3544 335
Size: 784 245 1521
Fragments arranged by size:
1521 784 411 399 379 360 314 245
176 115 26
NaeI GCC'GGC
Cuts at: 129 1176 2911 129
Size: 1047 1735 1948
Fragments arranged by size:
1948 1735 1047
NarI GG'CG_CC
Cuts at: 1057 2161 2409 1057
Size: 1104 248 3378
Fragments arranged by size:
3378 1104 248
NgoMIV G'CCGG_C
Cuts at: 127 1174 2909 127
Size: 1047 1735 1948
Fragments arranged by size:
1948 1735 1047
NsiI A_TGCA'T
Cuts at: 1150 1150
Size: 4730
NspI r_CATG'y
Cuts at: 573 727 1148 1783 2283 3886 573
Size: 154 421 635 500 1603 1417
Fragments arranged by size:
1603 1417 635 500 421 154
PasI CC'CwG_GG
Cuts at: 166 166
Size: 4730
PciI A'CATG_T
Cuts at: 3882 3882
Size: 4730
PfoI T'CCnGG_A
Cuts at: 386 923 1109 386
Size: 537 186 4007
Fragments arranged by size:
4007 537 186
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PmlI CAC'GTG
Cuts at: 684 684
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4698
PpuMI rG'GwC_Cy
Cuts at: 162 1345 1433 162
Size: 1183 88 3459
Fragments arranged by size:
3459 1183 88
PspOMI G'GGCC_C
Cuts at: 991 1027 1262 1283 1472 991
Size: 36 235 21 189 4249
Fragments arranged by size:
4249 235 189 36 21
PstI C_TGCA'G
Cuts at: 493 1199 1462 1481 1777 2462 493
Size: 706 263 19 296 685 2761
Fragments arranged by size:
2761 706 685 296 263 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 1063 2155 2515 1063
Size: 1092 360 3278
Fragments arranged by size:
3278 1092 360
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1744 1744
Size: 4730
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 155 1072 2753 2963 155
Size: 917 1681 210 1922
Fragments arranged by size:
1922 1681 917 210
SbfI CC_TGCA'GG
Cuts at: 1462 1481 1777 1462
Size: 19 296 4415
Fragments arranged by size:
4415 296 19
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SfcI C'TryA_G
Cuts at: 33 489 675 1195 1458 1477 1773 2458 3426
Size: 456 186 520 263 19 296 685 968
Cuts at: 3426 3617 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 520 456 296 263 191
186 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 776 776
Size: 4730
SfoI GGC'GCC
Cuts at: 1058 2162 2410 1058
Size: 1104 248 3378
Fragments arranged by size:
3378 1104 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SgrAI Cr'CCGG_yG
Cuts at: 1174 1174
Size: 4730
SmaI CCC'GGG
Cuts at: 990 1122 1220 1261 1267 1319 1418 1752 990
Size: 132 98 41 6 52 99 334 3968
Fragments arranged by size:
3968 334 132 99 98 52 41 6
SmlI C'TyrA_G
Cuts at: 198 1885 2800 3514 3776 198
Size: 1687 915 714 262 1152
Fragments arranged by size:
1687 1152 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 1783 2283 1783
Size: 500 4230
SrfI GCCC'GGGC
Cuts at: 1267 1418 1267
Size: 151 4579
StuI AGG'CCT
Cuts at: 709 779 709
Size: 70 4660
StyI C'CwwG_G
Cuts at: 284 959 1340 1705 1864 2218 284
Size: 675 381 365 159 354 2796
Fragments arranged by size:
2796 675 381 365 354 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 1227 2313 2446 2905 3114 226
Size: 1001 1086 133 459 209 1842
Fragments arranged by size:
1842 1086 1001 459 209 133
TatI w'GTAC_w
Cuts at: 4245 4245
Size: 4730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 4247 4279 172
Size: 32 4043 32 623
Fragments arranged by size:
4043 623 32 32
Tth111I GACn'n_nGTC
Cuts at: 1638 2527 1638
Size: 889 3841
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 616 2225 616
Size: 1609 3121
XmaI C'CCGG_G
Cuts at: 988 1120 1218 1259 1265 1317 1416 1750 988
Size: 132 98 41 6 52 99 334 3968
Fragments arranged by size:
3968 334 132 99 98 52 41 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AscI AvrII
BbvCI BcgI BmgBI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PflMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SnaBI SspI SwaI XhoI
XmnI ZraI