Restriction map for
pF1KSDF0136
(Circular) (Six-base) MAPSORT of: pF1KM.seq Check: 3141 from: 1 to: 3452
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 245 245
Size: 3452
AccI GT'mk_AC
Cuts at: 488 488
Size: 3452
Acc65I G'GTAC_C
Cuts at: 466 466
Size: 3452
AclI AA'CG_TT
Cuts at: 3292 3292
Size: 3452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 339 1275 1707 2456 2724 136
Size: 203 936 432 749 268 864
Fragments arranged by size:
936 864 749 432 268 203
AflIII A'CryG_T
Cuts at: 125 802 1908 2885 125
Size: 677 1106 977 692
Fragments arranged by size:
1106 977 692 677
AgeI A'CCGG_T
Cuts at: 1840 1840
Size: 3452
AlwNI CAG_nnn'CTG
Cuts at: 755 2324 755
Size: 1569 1883
ApaLI G'TGCA_C
Cuts at: 2222 2222
Size: 3452
ApoI r'AATT_y
Cuts at: 360 454 2805 3061 360
Size: 94 2351 256 751
Fragments arranged by size:
2351 751 256 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
AvaI C'yCGr_G
Cuts at: 470 470
Size: 3452
BamHI G'GATC_C
Cuts at: 475 475
Size: 3452
BanI G'GyrC_C
Cuts at: 466 880 1128 1163 466
Size: 414 248 35 2755
Fragments arranged by size:
2755 414 248 35
BanII G_rGCy'C
Cuts at: 464 1494 464
Size: 1030 2422
BbeI G_GCGC'C
Cuts at: 884 1132 884
Size: 248 3204
BciVI GTATCCnnnnn_n'
Cuts at: 1348 2111 3452 1348
Size: 763 1341 1348
Fragments arranged by size:
1348 1341 763
BclI T'GATC_A
Cuts at: 970 970
Size: 3452
BglII A'GATC_T
Cuts at: 965 3004 965
Size: 2039 1413
BlpI GC'TnA_GC
Cuts at: 562 562
Size: 3452
Bme1580I G_kGCm'C
Cuts at: 1075 1168 2226 1075
Size: 93 1058 2301
Fragments arranged by size:
2301 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 662 743 1078 3224 662
Size: 81 335 2146 890
Fragments arranged by size:
2146 890 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1817 1817
Size: 3452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 283
Size: 3452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 626 1505 1999 2297 366
Size: 260 879 494 298 1521
Fragments arranged by size:
1521 879 494 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 3156 3156
Size: 3452
BsaAI yAC'GTr
Cuts at: 803 803
Size: 3452
BsaBI GATnn'nnATC
Cuts at: 6 964 3003 6
Size: 958 2039 455
Fragments arranged by size:
2039 958 455
BsaHI Gr'CG_yC
Cuts at: 242 881 1129 1833 3329 242
Size: 639 248 704 1496 365
Fragments arranged by size:
1496 704 639 365 248
BsaWI w'CCGG_w
Cuts at: 368 639 1160 1840 2114 2261 2672 368
Size: 271 521 680 274 147 411 1148
Fragments arranged by size:
1148 680 521 411 274 271 147
BseYI C'CCAG_C
Cuts at: 212 875 1633 2212 2708 212
Size: 663 758 579 496 956
Fragments arranged by size:
956 758 663 579 496
BsiEI CG_ry'CG
Cuts at: 104 992 1038 2248 104
Size: 888 46 1210 1308
Fragments arranged by size:
1308 1210 888 46
BsiHKAI G_wGCw'C
Cuts at: 464 1242 1432 2226 464
Size: 778 190 794 1690
Fragments arranged by size:
1690 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 464 1075 1168 1242 1432 1494 2226 464
Size: 611 93 74 190 62 732 1690
Fragments arranged by size:
1690 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2672 2672
Size: 3452
BspHI T'CATG_A
Cuts at: 2628 2628
Size: 3452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 714 1016 1397 500
Size: 214 302 381 2555
Fragments arranged by size:
2555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1742 1796 3282 3447 50
Size: 1692 54 1486 165 55
Fragments arranged by size:
1692 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1362 1362
Size: 3452
BsrFI r'CCGG_y
Cuts at: 1448 1629 1840 1448
Size: 181 211 3060
Fragments arranged by size:
3060 211 181
BssSI C'ACGA_G
Cuts at: 1721 2081 1721
Size: 360 3092
BstAPI GCAn_nnn'nTGC
Cuts at: 827 827
Size: 3452
BstYI r'GATC_y
Cuts at: 1 475 942 965 1300 1546 2560 2646 2658
Size: 474 467 23 335 246 1014 86 12
Cuts at: 2658 3004 1
Size: 346 449
Fragments arranged by size:
1014 474 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1400 1581 1744 1400
Size: 181 163 3108
Fragments arranged by size:
3108 181 163
BtsI GCAGTG_nn'
Cuts at: 693 693
Size: 3452
DraI TTT'AAA
Cuts at: 449 449
Size: 3452
DrdI GACnn_nn'nnGTC
Cuts at: 1156 2016 1156
Size: 860 2592
EaeI y'GGCC_r
Cuts at: 1035 1209 1600 1627 2974 1035
Size: 174 391 27 1347 1513
Fragments arranged by size:
1513 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1035 1035
Size: 3452
EarI CTCTTCn'nnn_
Cuts at: 1473 1683 1473
Size: 210 3242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 281 1658 1970 2116 3262 281
Size: 1377 312 146 1146 471
Fragments arranged by size:
1377 1146 471 312 146
EcoICRI GAG'CTC
Cuts at: 462 462
Size: 3452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 283 339 1275 1707 2456 2724 136
Size: 147 56 936 432 749 268 864
Fragments arranged by size:
936 864 749 432 268 147 56
EcoO109I rG'GnC_Cy
Cuts at: 589 589
Size: 3452
EcoRI G'AATT_C
Cuts at: 454 454
Size: 3452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 250 1469 250
Size: 1219 2233
FspI TGC'GCA
Cuts at: 1231 1231
Size: 3452
HaeII r_GCGC'y
Cuts at: 884 1132 2156 3135 884
Size: 248 1024 979 1201
Fragments arranged by size:
1201 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 1292 1322 1324 1354 262
Size: 32 998 30 2 30 2360
Fragments arranged by size:
2360 998 32 30 30 2
HincII GTy'rAC
Cuts at: 489 489
Size: 3452
KasI G'GCGC_C
Cuts at: 880 1128 880
Size: 248 3204
KpnI G_GTAC'C
Cuts at: 470 470
Size: 3452
MluI A'CGCG_T
Cuts at: 2885 2885
Size: 3452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2307 2123
Size: 184 3268
MscI TGG'CCA
Cuts at: 1211 1211
Size: 3452
MslI CAynn'nnrTG
Cuts at: 1439 1566 1439
Size: 127 3325
MspA1I CmG'CkG
Cuts at: 561 875 1235 2250 2495 561
Size: 314 360 1015 245 1518
Fragments arranged by size:
1518 1015 360 314 245
NaeI GCC'GGC
Cuts at: 1631 1631
Size: 3452
NarI GG'CG_CC
Cuts at: 881 1129 881
Size: 248 3204
NcoI C'CATG_G
Cuts at: 107 107
Size: 3452
NgoMIV G'CCGG_C
Cuts at: 1629 1629
Size: 3452
NruI TCG'CGA
Cuts at: 1813 1813
Size: 3452
NspI r_CATG'y
Cuts at: 503 1003 1912 503
Size: 500 909 2043
Fragments arranged by size:
2043 909 500
PciI A'CATG_T
Cuts at: 1908 1908
Size: 3452
PmeI GTTT'AAAC
Cuts at: 449 449
Size: 3452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 3420
PstI C_TGCA'G
Cuts at: 497 1182 497
Size: 685 2767
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3452
PvuII CAG'CTG
Cuts at: 875 1235 875
Size: 360 3092
RsrII CG'GwC_CG
Cuts at: 1645 1645
Size: 3452
SacI G_AGCT'C
Cuts at: 464 464
Size: 3452
SalI G'TCGA_C
Cuts at: 487 487
Size: 3452
SapI GCTCTTCn'nnn_
Cuts at: 1473 1683 1473
Size: 210 3242
SbfI CC_TGCA'GG
Cuts at: 497 497
Size: 3452
ScaI AGT'ACT
Cuts at: 2969 2969
Size: 3452
SfcI C'TryA_G
Cuts at: 33 493 1178 2173 2364 33
Size: 460 685 995 191 1121
Fragments arranged by size:
1121 995 685 460 191
SfoI GGC'GCC
Cuts at: 882 1130 882
Size: 248 3204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
SmaI CCC'GGG
Cuts at: 472 472
Size: 3452
SmlI C'TyrA_G
Cuts at: 381 605 1520 2014 2276 381
Size: 224 915 494 262 1557
Fragments arranged by size:
1557 915 494 262 224
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3452
SphI G_CATG'C
Cuts at: 503 1003 503
Size: 500 2952
StyI C'CwwG_G
Cuts at: 107 584 938 107
Size: 477 354 2621
Fragments arranged by size:
2621 477 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 1033 1166 1625 1836 204
Size: 829 133 459 211 1820
Fragments arranged by size:
1820 829 459 211 133
TatI w'GTAC_w
Cuts at: 2967 2967
Size: 3452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3420
Tth111I GACn'n_nGTC
Cuts at: 1247 1247
Size: 3452
XbaI T'CTAG_A
Cuts at: 481 481
Size: 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 945
Size: 3452
XmaI C'CCGG_G
Cuts at: 470 470
Size: 3452
ZraI GAC'GTC
Cuts at: 243 243
Size: 3452
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI