Restriction map for
pF1KSDA0007
(Circular) (Six-base) MAPSORT of: pF1KSDA0007.seq Check: 505 from: 1 to: 5168
pF1KSDA0007 5168 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 725 2206 725
Size: 1481 3687
Acc65I G'GTAC_C
Cuts at: 304 1758 2184 304
Size: 1454 426 3288
Fragments arranged by size:
3288 1454 426
AclI AA'CG_TT
Cuts at: 5008 5008
Size: 5168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1343 1958 1997 2993 3425 4172 4440 1343
Size: 615 39 996 432 747 268 2071
Fragments arranged by size:
2071 996 747 615 432 268 39
AflIII A'CryG_T
Cuts at: 2520 3624 4601 2520
Size: 1104 977 3087
Fragments arranged by size:
3087 1104 977
AgeI A'CCGG_T
Cuts at: 854 3556 854
Size: 2702 2466
AhdI GACnn_n'nnGTC
Cuts at: 1158 1158
Size: 5168
AleI CACnn'nnGTG
Cuts at: 299 299
Size: 5168
AlwNI CAG_nnn'CTG
Cuts at: 385 542 2473 4040 385
Size: 157 1931 1567 1513
Fragments arranged by size:
1931 1567 1513 157
ApaI G_GGCC'C
Cuts at: 1272 1272
Size: 5168
ApaLI G'TGCA_C
Cuts at: 3938 3938
Size: 5168
ApoI r'AATT_y
Cuts at: 1317 2172 4521 4777 1317
Size: 855 2349 256 1708
Fragments arranged by size:
2349 1708 855 256
AseI AT'TA_AT
Cuts at: 20 484 20
Size: 464 4704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5168
AvaI C'yCGr_G
Cuts at: 2188 2188
Size: 5168
BamHI G'GATC_C
Cuts at: 2193 2193
Size: 5168
BanI G'GyrC_C
Cuts at: 304 327 1338 1758 2184 2598 2846 2881 304
Size: 23 1011 420 426 414 248 35 2591
Fragments arranged by size:
2591 1011 426 420 414 248 35 23
BanII G_rGCy'C
Cuts at: 1272 2182 3212 1272
Size: 910 1030 3228
Fragments arranged by size:
3228 1030 910
BbeI G_GCGC'C
Cuts at: 331 2602 2850 331
Size: 2271 248 2649
Fragments arranged by size:
2649 2271 248
BbsI GAAGACnn'nnnn_
Cuts at: 1496 1606 1882 1925 1496
Size: 110 276 43 4739
Fragments arranged by size:
4739 276 110 43
BciVI GTATCCnnnnn_n'
Cuts at: 3066 3827 5168 3066
Size: 761 1341 3066
Fragments arranged by size:
3066 1341 761
BclI T'GATC_A
Cuts at: 2688 2688
Size: 5168
BfrBI ATG'CAT
Cuts at: 1729 1729
Size: 5168
BglI GCCn_nnn'nGGC
Cuts at: 336 336
Size: 5168
BglII A'GATC_T
Cuts at: 2683 4720 2683
Size: 2037 3131
BlpI GC'TnA_GC
Cuts at: 2280 2280
Size: 5168
Bme1580I G_kGCm'C
Cuts at: 1272 2793 2886 3942 1272
Size: 1521 93 1056 2498
Fragments arranged by size:
2498 1521 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2380 2461 2796 4940 2380
Size: 81 335 2144 2608
Fragments arranged by size:
2608 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3535 3535
Size: 5168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 860 1206 1480 860
Size: 346 274 4548
Fragments arranged by size:
4548 346 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 3223 3715 4013 2344
Size: 879 492 298 3499
Fragments arranged by size:
3499 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 706 4872 706
Size: 4166 1002
BsaAI yAC'GTr
Cuts at: 282 2160 2521 282
Size: 1878 361 2929
Fragments arranged by size:
2929 1878 361
BsaBI GATnn'nnATC
Cuts at: 6 2682 4719 6
Size: 2676 2037 455
Fragments arranged by size:
2676 2037 455
BsaHI Gr'CG_yC
Cuts at: 328 2599 2847 3549 5045 328
Size: 2271 248 702 1496 451
Fragments arranged by size:
2271 1496 702 451 248
BsaWI w'CCGG_w
Cuts at: 854 2357 2878 3556 3830 3977 4388 854
Size: 1503 521 678 274 147 411 1634
Fragments arranged by size:
1634 1503 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1370 1957 2014 2017 1370
Size: 587 57 3 4521
Fragments arranged by size:
4521 587 57 3
BseYI C'CCAG_C
Cuts at: 169 1265 1763 1767 2593 3351 3928 4424 169
Size: 1096 498 4 826 758 577 496 913
Fragments arranged by size:
1096 913 826 758 577 498 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 294 923 1053 253
Size: 41 629 130 4368
Fragments arranged by size:
4368 629 130 41
BsiEI CG_ry'CG
Cuts at: 104 2710 2756 3964 104
Size: 2606 46 1208 1308
Fragments arranged by size:
2606 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2182 2960 3150 3942 2182
Size: 778 190 792 3408
Fragments arranged by size:
3408 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 244 244
Size: 5168
Bsp1286I G_dGCh'C
Cuts at: 1272 2182 2793 2886 2960 3150 3212 3942 1272
Size: 910 611 93 74 190 62 730 2498
Fragments arranged by size:
2498 910 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4388 4388
Size: 5168
BspHI T'CATG_A
Cuts at: 4344 4344
Size: 5168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 316 864 2218 2432 2734 3115 316
Size: 548 1354 214 302 381 2369
Fragments arranged by size:
2369 1354 548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1378 3460 3514 4998 5163 50
Size: 1328 2082 54 1484 165 55
Fragments arranged by size:
2082 1484 1328 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 3080 1463
Size: 1617 3551
BsrFI r'CCGG_y
Cuts at: 263 854 3166 3347 3556 263
Size: 591 2312 181 209 1875
Fragments arranged by size:
2312 1875 591 209 181
BssSI C'ACGA_G
Cuts at: 3439 3797 3439
Size: 358 4810
BstAPI GCAn_nnn'nTGC
Cuts at: 2545 2545
Size: 5168
BstBI TT'CG_AA
Cuts at: 110 3529 110
Size: 3419 1749
BstYI r'GATC_y
Cuts at: 1 2130 2193 2660 2683 3018 3264 4276 4362
Size: 2129 63 467 23 335 246 1012 86
Cuts at: 4362 4374 4720 1
Size: 12 346 449
Fragments arranged by size:
2129 1012 467 449 346 335 246 86
63 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3118 3299 3462 3118
Size: 181 163 4824
Fragments arranged by size:
4824 181 163
BtsI GCAGTG_nn'
Cuts at: 639 911 2411 639
Size: 272 1500 3396
Fragments arranged by size:
3396 1500 272
DraI TTT'AAA
Cuts at: 1217 2167 1217
Size: 950 4218
DrdI GACnn_nn'nnGTC
Cuts at: 508 2874 3732 508
Size: 2366 858 1944
Fragments arranged by size:
2366 1944 858
EaeI y'GGCC_r
Cuts at: 254 2753 2927 3318 3345 4690 254
Size: 2499 174 391 27 1345 732
Fragments arranged by size:
2499 1345 732 391 174 27
EagI C'GGCC_G
Cuts at: 2753 2753
Size: 5168
EarI CTCTTCn'nnn_
Cuts at: 965 1922 1934 2030 3191 3401 965
Size: 957 12 96 1161 210 2732
Fragments arranged by size:
2732 1161 957 210 96 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3376 3686 3832 4978 3376
Size: 310 146 1146 3566
Fragments arranged by size:
3566 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2180 2180
Size: 5168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 860 1206 1343 1480 1958 1997 2993 3425 4172
Size: 346 137 137 478 39 996 432 747
Cuts at: 4172 4440 860
Size: 268 1588
Fragments arranged by size:
1588 996 747 478 432 346 268 137
137 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1094 1094
Size: 5168
EcoO109I rG'GnC_Cy
Cuts at: 174 1269 2307 174
Size: 1095 1038 3035
Fragments arranged by size:
3035 1095 1038
EcoRI G'AATT_C
Cuts at: 1317 2172 1317
Size: 855 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1033 3187 1033
Size: 2154 3014
FspI TGC'GCA
Cuts at: 2949 2949
Size: 5168
HaeII r_GCGC'y
Cuts at: 331 2602 2850 3872 4851 331
Size: 2271 248 1022 979 648
Fragments arranged by size:
2271 1022 979 648 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 1338 1370 3010 3040 3042 3072 1144
Size: 32 162 32 1640 30 2 30 3240
Fragments arranged by size:
3240 1640 162 32 32 30 30 2
HincII GTy'rAC
Cuts at: 513 1109 1718 2207 513
Size: 596 609 489 3474
Fragments arranged by size:
3474 609 596 489
HindIII A'AGCT_T
Cuts at: 1837 1837
Size: 5168
HpaI GTT'AAC
Cuts at: 1718 1718
Size: 5168
KasI G'GCGC_C
Cuts at: 327 2598 2846 327
Size: 2271 248 2649
Fragments arranged by size:
2649 2271 248
KpnI G_GTAC'C
Cuts at: 308 1762 2188 308
Size: 1454 426 3288
Fragments arranged by size:
3288 1454 426
MluI A'CGCG_T
Cuts at: 4601 4601
Size: 5168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3839 4023 3839
Size: 184 4984
MscI TGG'CCA
Cuts at: 2929 2929
Size: 5168
MslI CAynn'nnrTG
Cuts at: 299 1829 3157 3284 299
Size: 1530 1328 127 2183
Fragments arranged by size:
2183 1530 1328 127
MspA1I CmG'CkG
Cuts at: 682 1767 2279 2593 2953 3966 4211 682
Size: 1085 512 314 360 1013 245 1639
Fragments arranged by size:
1639 1085 1013 512 360 314 245
NaeI GCC'GGC
Cuts at: 3349 3349
Size: 5168
NarI GG'CG_CC
Cuts at: 328 2599 2847 328
Size: 2271 248 2649
Fragments arranged by size:
2649 2271 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5168
NdeI CA'TA_TG
Cuts at: 1235 1235
Size: 5168
NgoMIV G'CCGG_C
Cuts at: 3347 3347
Size: 5168
NsiI A_TGCA'T
Cuts at: 1731 1731
Size: 5168
NspI r_CATG'y
Cuts at: 599 748 2221 2721 3628 599
Size: 149 1473 500 907 2139
Fragments arranged by size:
2139 1473 907 500 149
PasI CC'CwG_GG
Cuts at: 1527 1527
Size: 5168
PciI A'CATG_T
Cuts at: 3624 3624
Size: 5168
PflMI CCAn_nnn'nTGG
Cuts at: 1788 1788
Size: 5168
PmeI GTTT'AAAC
Cuts at: 2167 2167
Size: 5168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 4307
Size: 32 5136
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 5168
PsiI TTA'TAA
Cuts at: 1594 1594
Size: 5168
PspOMI G'GGCC_C
Cuts at: 1268 1268
Size: 5168
PstI C_TGCA'G
Cuts at: 344 2215 2900 344
Size: 1871 685 2612
Fragments arranged by size:
2612 1871 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5168
PvuII CAG'CTG
Cuts at: 682 1767 2593 2953 682
Size: 1085 826 360 2897
Fragments arranged by size:
2897 1085 826 360
RsrII CG'GwC_CG
Cuts at: 3363 3363
Size: 5168
SacI G_AGCT'C
Cuts at: 2182 2182
Size: 5168
SalI G'TCGA_C
Cuts at: 2205 2205
Size: 5168
SanDI GG'GwC_CC
Cuts at: 174 174
Size: 5168
SapI GCTCTTCn'nnn_
Cuts at: 965 3191 3401 965
Size: 2226 210 2732
Fragments arranged by size:
2732 2226 210
SbfI CC_TGCA'GG
Cuts at: 2215 2215
Size: 5168
ScaI AGT'ACT
Cuts at: 1568 4685 1568
Size: 3117 2051
SexAI A'CCwGG_T
Cuts at: 1754 1754
Size: 5168
SfcI C'TryA_G
Cuts at: 33 340 386 726 2211 2896 3889 4080 33
Size: 307 46 340 1485 685 993 191 1121
Fragments arranged by size:
1485 1121 993 685 340 307 191 46
SfoI GGC'GCC
Cuts at: 329 2600 2848 329
Size: 2271 248 2649
Fragments arranged by size:
2649 2271 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5168
SmaI CCC'GGG
Cuts at: 2190 2190
Size: 5168
SmlI C'TyrA_G
Cuts at: 2323 3238 3730 3992 2323
Size: 915 492 262 3499
Fragments arranged by size:
3499 915 492 262
SnaBI TAC'GTA
Cuts at: 2160 2160
Size: 5168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5168
SphI G_CATG'C
Cuts at: 2221 2721 2221
Size: 500 4668
StuI AGG'CCT
Cuts at: 205 205
Size: 5168
StyI C'CwwG_G
Cuts at: 125 347 2302 2656 125
Size: 222 1955 354 2637
Fragments arranged by size:
2637 1955 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2751 2884 3343 3552 2751
Size: 133 459 209 4367
Fragments arranged by size:
4367 459 209 133
TatI w'GTAC_w
Cuts at: 846 1566 4683 846
Size: 720 3117 1331
Fragments arranged by size:
3117 1331 720
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4685 4717 4685
Size: 32 5136
Tth111I GACn'n_nGTC
Cuts at: 2965 2965
Size: 5168
XbaI T'CTAG_A
Cuts at: 2199 2199
Size: 5168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 808 2663 808
Size: 1855 3313
XmaI C'CCGG_G
Cuts at: 2188 2188
Size: 5168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI MfeI NheI NotI
NruI PacI PfoI PmlI PshAI PspXI PsrI SacII
SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI