(Circular) (Six-base) MAPSORT of: pF1KSDA0007.seq Check: 505 from: 1 to: 5168 pF1KSDA0007 5168 bp With 182 enzymes: SgfI * June 20, 2008 10:32 .. AccI GT'mk_AC Cuts at: 725 2206 725 Size: 1481 3687 Acc65I G'GTAC_C Cuts at: 304 1758 2184 304 Size: 1454 426 3288 Fragments arranged by size: 3288 1454 426 AclI AA'CG_TT Cuts at: 5008 5008 Size: 5168 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1343 1958 1997 2993 3425 4172 4440 1343 Size: 615 39 996 432 747 268 2071 Fragments arranged by size: 2071 996 747 615 432 268 39 AflIII A'CryG_T Cuts at: 2520 3624 4601 2520 Size: 1104 977 3087 Fragments arranged by size: 3087 1104 977 AgeI A'CCGG_T Cuts at: 854 3556 854 Size: 2702 2466 AhdI GACnn_n'nnGTC Cuts at: 1158 1158 Size: 5168 AleI CACnn'nnGTG Cuts at: 299 299 Size: 5168 AlwNI CAG_nnn'CTG Cuts at: 385 542 2473 4040 385 Size: 157 1931 1567 1513 Fragments arranged by size: 1931 1567 1513 157 ApaI G_GGCC'C Cuts at: 1272 1272 Size: 5168 ApaLI G'TGCA_C Cuts at: 3938 3938 Size: 5168 ApoI r'AATT_y Cuts at: 1317 2172 4521 4777 1317 Size: 855 2349 256 1708 Fragments arranged by size: 2349 1708 855 256 AseI AT'TA_AT Cuts at: 20 484 20 Size: 464 4704 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5168 AvaI C'yCGr_G Cuts at: 2188 2188 Size: 5168 BamHI G'GATC_C Cuts at: 2193 2193 Size: 5168 BanI G'GyrC_C Cuts at: 304 327 1338 1758 2184 2598 2846 2881 304 Size: 23 1011 420 426 414 248 35 2591 Fragments arranged by size: 2591 1011 426 420 414 248 35 23 BanII G_rGCy'C Cuts at: 1272 2182 3212 1272 Size: 910 1030 3228 Fragments arranged by size: 3228 1030 910 BbeI G_GCGC'C Cuts at: 331 2602 2850 331 Size: 2271 248 2649 Fragments arranged by size: 2649 2271 248 BbsI GAAGACnn'nnnn_ Cuts at: 1496 1606 1882 1925 1496 Size: 110 276 43 4739 Fragments arranged by size: 4739 276 110 43 BciVI GTATCCnnnnn_n' Cuts at: 3066 3827 5168 3066 Size: 761 1341 3066 Fragments arranged by size: 3066 1341 761 BclI T'GATC_A Cuts at: 2688 2688 Size: 5168 BfrBI ATG'CAT Cuts at: 1729 1729 Size: 5168 BglI GCCn_nnn'nGGC Cuts at: 336 336 Size: 5168 BglII A'GATC_T Cuts at: 2683 4720 2683 Size: 2037 3131 BlpI GC'TnA_GC Cuts at: 2280 2280 Size: 5168 Bme1580I G_kGCm'C Cuts at: 1272 2793 2886 3942 1272 Size: 1521 93 1056 2498 Fragments arranged by size: 2498 1521 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2380 2461 2796 4940 2380 Size: 81 335 2144 2608 Fragments arranged by size: 2608 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3535 3535 Size: 5168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 860 1206 1480 860 Size: 346 274 4548 Fragments arranged by size: 4548 346 274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2344 3223 3715 4013 2344 Size: 879 492 298 3499 Fragments arranged by size: 3499 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 706 4872 706 Size: 4166 1002 BsaAI yAC'GTr Cuts at: 282 2160 2521 282 Size: 1878 361 2929 Fragments arranged by size: 2929 1878 361 BsaBI GATnn'nnATC Cuts at: 6 2682 4719 6 Size: 2676 2037 455 Fragments arranged by size: 2676 2037 455 BsaHI Gr'CG_yC Cuts at: 328 2599 2847 3549 5045 328 Size: 2271 248 702 1496 451 Fragments arranged by size: 2271 1496 702 451 248 BsaWI w'CCGG_w Cuts at: 854 2357 2878 3556 3830 3977 4388 854 Size: 1503 521 678 274 147 411 1634 Fragments arranged by size: 1634 1503 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1370 1957 2014 2017 1370 Size: 587 57 3 4521 Fragments arranged by size: 4521 587 57 3 BseYI C'CCAG_C Cuts at: 169 1265 1763 1767 2593 3351 3928 4424 169 Size: 1096 498 4 826 758 577 496 913 Fragments arranged by size: 1096 913 826 758 577 498 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 253 294 923 1053 253 Size: 41 629 130 4368 Fragments arranged by size: 4368 629 130 41 BsiEI CG_ry'CG Cuts at: 104 2710 2756 3964 104 Size: 2606 46 1208 1308 Fragments arranged by size: 2606 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2182 2960 3150 3942 2182 Size: 778 190 792 3408 Fragments arranged by size: 3408 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 244 244 Size: 5168 Bsp1286I G_dGCh'C Cuts at: 1272 2182 2793 2886 2960 3150 3212 3942 1272 Size: 910 611 93 74 190 62 730 2498 Fragments arranged by size: 2498 910 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 4388 4388 Size: 5168 BspHI T'CATG_A Cuts at: 4344 4344 Size: 5168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 316 864 2218 2432 2734 3115 316 Size: 548 1354 214 302 381 2369 Fragments arranged by size: 2369 1354 548 381 302 214 BsrBI CCG'CTC Cuts at: 50 1378 3460 3514 4998 5163 50 Size: 1328 2082 54 1484 165 55 Fragments arranged by size: 2082 1484 1328 165 55 54 BsrDI GCAATG_nn' Cuts at: 1463 3080 1463 Size: 1617 3551 BsrFI r'CCGG_y Cuts at: 263 854 3166 3347 3556 263 Size: 591 2312 181 209 1875 Fragments arranged by size: 2312 1875 591 209 181 BssSI C'ACGA_G Cuts at: 3439 3797 3439 Size: 358 4810 BstAPI GCAn_nnn'nTGC Cuts at: 2545 2545 Size: 5168 BstBI TT'CG_AA Cuts at: 110 3529 110 Size: 3419 1749 BstYI r'GATC_y Cuts at: 1 2130 2193 2660 2683 3018 3264 4276 4362 Size: 2129 63 467 23 335 246 1012 86 Cuts at: 4362 4374 4720 1 Size: 12 346 449 Fragments arranged by size: 2129 1012 467 449 346 335 246 86 63 23 12 BtgI C'CryG_G Cuts at: 125 125 Size: 5168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3118 3299 3462 3118 Size: 181 163 4824 Fragments arranged by size: 4824 181 163 BtsI GCAGTG_nn' Cuts at: 639 911 2411 639 Size: 272 1500 3396 Fragments arranged by size: 3396 1500 272 DraI TTT'AAA Cuts at: 1217 2167 1217 Size: 950 4218 DrdI GACnn_nn'nnGTC Cuts at: 508 2874 3732 508 Size: 2366 858 1944 Fragments arranged by size: 2366 1944 858 EaeI y'GGCC_r Cuts at: 254 2753 2927 3318 3345 4690 254 Size: 2499 174 391 27 1345 732 Fragments arranged by size: 2499 1345 732 391 174 27 EagI C'GGCC_G Cuts at: 2753 2753 Size: 5168 EarI CTCTTCn'nnn_ Cuts at: 965 1922 1934 2030 3191 3401 965 Size: 957 12 96 1161 210 2732 Fragments arranged by size: 2732 1161 957 210 96 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3376 3686 3832 4978 3376 Size: 310 146 1146 3566 Fragments arranged by size: 3566 1146 310 146 EcoICRI GAG'CTC Cuts at: 2180 2180 Size: 5168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 860 1206 1343 1480 1958 1997 2993 3425 4172 Size: 346 137 137 478 39 996 432 747 Cuts at: 4172 4440 860 Size: 268 1588 Fragments arranged by size: 1588 996 747 478 432 346 268 137 137 39 EcoNI CCTnn'n_nnAGG Cuts at: 1094 1094 Size: 5168 EcoO109I rG'GnC_Cy Cuts at: 174 1269 2307 174 Size: 1095 1038 3035 Fragments arranged by size: 3035 1095 1038 EcoRI G'AATT_C Cuts at: 1317 2172 1317 Size: 855 4313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1033 3187 1033 Size: 2154 3014 FspI TGC'GCA Cuts at: 2949 2949 Size: 5168 HaeII r_GCGC'y Cuts at: 331 2602 2850 3872 4851 331 Size: 2271 248 1022 979 648 Fragments arranged by size: 2271 1022 979 648 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1144 1176 1338 1370 3010 3040 3042 3072 1144 Size: 32 162 32 1640 30 2 30 3240 Fragments arranged by size: 3240 1640 162 32 32 30 30 2 HincII GTy'rAC Cuts at: 513 1109 1718 2207 513 Size: 596 609 489 3474 Fragments arranged by size: 3474 609 596 489 HindIII A'AGCT_T Cuts at: 1837 1837 Size: 5168 HpaI GTT'AAC Cuts at: 1718 1718 Size: 5168 KasI G'GCGC_C Cuts at: 327 2598 2846 327 Size: 2271 248 2649 Fragments arranged by size: 2649 2271 248 KpnI G_GTAC'C Cuts at: 308 1762 2188 308 Size: 1454 426 3288 Fragments arranged by size: 3288 1454 426 MluI A'CGCG_T Cuts at: 4601 4601 Size: 5168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3839 4023 3839 Size: 184 4984 MscI TGG'CCA Cuts at: 2929 2929 Size: 5168 MslI CAynn'nnrTG Cuts at: 299 1829 3157 3284 299 Size: 1530 1328 127 2183 Fragments arranged by size: 2183 1530 1328 127 MspA1I CmG'CkG Cuts at: 682 1767 2279 2593 2953 3966 4211 682 Size: 1085 512 314 360 1013 245 1639 Fragments arranged by size: 1639 1085 1013 512 360 314 245 NaeI GCC'GGC Cuts at: 3349 3349 Size: 5168 NarI GG'CG_CC Cuts at: 328 2599 2847 328 Size: 2271 248 2649 Fragments arranged by size: 2649 2271 248 NcoI C'CATG_G Cuts at: 125 125 Size: 5168 NdeI CA'TA_TG Cuts at: 1235 1235 Size: 5168 NgoMIV G'CCGG_C Cuts at: 3347 3347 Size: 5168 NsiI A_TGCA'T Cuts at: 1731 1731 Size: 5168 NspI r_CATG'y Cuts at: 599 748 2221 2721 3628 599 Size: 149 1473 500 907 2139 Fragments arranged by size: 2139 1473 907 500 149 PasI CC'CwG_GG Cuts at: 1527 1527 Size: 5168 PciI A'CATG_T Cuts at: 3624 3624 Size: 5168 PflMI CCAn_nnn'nTGG Cuts at: 1788 1788 Size: 5168 PmeI GTTT'AAAC Cuts at: 2167 2167 Size: 5168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 4307 Size: 32 5136 PpuMI rG'GwC_Cy Cuts at: 174 174 Size: 5168 PsiI TTA'TAA Cuts at: 1594 1594 Size: 5168 PspOMI G'GGCC_C Cuts at: 1268 1268 Size: 5168 PstI C_TGCA'G Cuts at: 344 2215 2900 344 Size: 1871 685 2612 Fragments arranged by size: 2612 1871 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5168 PvuII CAG'CTG Cuts at: 682 1767 2593 2953 682 Size: 1085 826 360 2897 Fragments arranged by size: 2897 1085 826 360 RsrII CG'GwC_CG Cuts at: 3363 3363 Size: 5168 SacI G_AGCT'C Cuts at: 2182 2182 Size: 5168 SalI G'TCGA_C Cuts at: 2205 2205 Size: 5168 SanDI GG'GwC_CC Cuts at: 174 174 Size: 5168 SapI GCTCTTCn'nnn_ Cuts at: 965 3191 3401 965 Size: 2226 210 2732 Fragments arranged by size: 2732 2226 210 SbfI CC_TGCA'GG Cuts at: 2215 2215 Size: 5168 ScaI AGT'ACT Cuts at: 1568 4685 1568 Size: 3117 2051 SexAI A'CCwGG_T Cuts at: 1754 1754 Size: 5168 SfcI C'TryA_G Cuts at: 33 340 386 726 2211 2896 3889 4080 33 Size: 307 46 340 1485 685 993 191 1121 Fragments arranged by size: 1485 1121 993 685 340 307 191 46 SfoI GGC'GCC Cuts at: 329 2600 2848 329 Size: 2271 248 2649 Fragments arranged by size: 2649 2271 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5168 SmaI CCC'GGG Cuts at: 2190 2190 Size: 5168 SmlI C'TyrA_G Cuts at: 2323 3238 3730 3992 2323 Size: 915 492 262 3499 Fragments arranged by size: 3499 915 492 262 SnaBI TAC'GTA Cuts at: 2160 2160 Size: 5168 SpeI A'CTAG_T Cuts at: 67 67 Size: 5168 SphI G_CATG'C Cuts at: 2221 2721 2221 Size: 500 4668 StuI AGG'CCT Cuts at: 205 205 Size: 5168 StyI C'CwwG_G Cuts at: 125 347 2302 2656 125 Size: 222 1955 354 2637 Fragments arranged by size: 2637 1955 354 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2751 2884 3343 3552 2751 Size: 133 459 209 4367 Fragments arranged by size: 4367 459 209 133 TatI w'GTAC_w Cuts at: 846 1566 4683 846 Size: 720 3117 1331 Fragments arranged by size: 3117 1331 720 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4685 4717 4685 Size: 32 5136 Tth111I GACn'n_nGTC Cuts at: 2965 2965 Size: 5168 XbaI T'CTAG_A Cuts at: 2199 2199 Size: 5168 XcmI CCAnnnn_n'nnnnTGG Cuts at: 808 2663 808 Size: 1855 3313 XmaI C'CCGG_G Cuts at: 2188 2188 Size: 5168 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AlfI AloI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI MfeI NheI NotI NruI PacI PfoI PmlI PshAI PspXI PsrI SacII SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI