Restriction map for pF1KSDA0007
 (Circular) (Six-base) MAPSORT of: pF1KSDA0007.seq  Check: 505  from: 1  to: 5168



pF1KSDA0007  5168 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    725    2206     725 
   Size:       1481    3687
 
Acc65I G'GTAC_C

Cuts at:    304    1758    2184     304 
   Size:       1454     426    3288

  Fragments arranged by size: 

                3288    1454     426
 
AclI AA'CG_TT

Cuts at:   5008    5008 
   Size:       5168
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1343    1958    1997    2993    3425    4172    4440    1343 
   Size:        615      39     996     432     747     268    2071

  Fragments arranged by size: 

                2071     996     747     615     432     268      39
 
AflIII A'CryG_T

Cuts at:   2520    3624    4601    2520 
   Size:       1104     977    3087

  Fragments arranged by size: 

                3087    1104     977
 
AgeI A'CCGG_T

Cuts at:    854    3556     854 
   Size:       2702    2466
 
AhdI GACnn_n'nnGTC

Cuts at:   1158    1158 
   Size:       5168
 
AleI CACnn'nnGTG

Cuts at:    299     299 
   Size:       5168
 
AlwNI CAG_nnn'CTG

Cuts at:    385     542    2473    4040     385 
   Size:        157    1931    1567    1513

  Fragments arranged by size: 

                1931    1567    1513     157
 
ApaI G_GGCC'C

Cuts at:   1272    1272 
   Size:       5168
 
ApaLI G'TGCA_C

Cuts at:   3938    3938 
   Size:       5168
 
ApoI r'AATT_y

Cuts at:   1317    2172    4521    4777    1317 
   Size:        855    2349     256    1708

  Fragments arranged by size: 

                2349    1708     855     256
 
AseI AT'TA_AT

Cuts at:     20     484      20 
   Size:        464    4704
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5168
 
AvaI C'yCGr_G

Cuts at:   2188    2188 
   Size:       5168
 
BamHI G'GATC_C

Cuts at:   2193    2193 
   Size:       5168
 
BanI G'GyrC_C

Cuts at:    304     327    1338    1758    2184    2598    2846    2881     304 
   Size:         23    1011     420     426     414     248      35    2591

  Fragments arranged by size: 

                2591    1011     426     420     414     248      35      23
            
 
BanII G_rGCy'C

Cuts at:   1272    2182    3212    1272 
   Size:        910    1030    3228

  Fragments arranged by size: 

                3228    1030     910
 
BbeI G_GCGC'C

Cuts at:    331    2602    2850     331 
   Size:       2271     248    2649

  Fragments arranged by size: 

                2649    2271     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1496    1606    1882    1925    1496 
   Size:        110     276      43    4739

  Fragments arranged by size: 

                4739     276     110      43
 
BciVI GTATCCnnnnn_n'

Cuts at:   3066    3827    5168    3066 
   Size:        761    1341    3066

  Fragments arranged by size: 

                3066    1341     761
 
BclI T'GATC_A

Cuts at:   2688    2688 
   Size:       5168
 
BfrBI ATG'CAT

Cuts at:   1729    1729 
   Size:       5168
 
BglI GCCn_nnn'nGGC

Cuts at:    336     336 
   Size:       5168
 
BglII A'GATC_T

Cuts at:   2683    4720    2683 
   Size:       2037    3131
 
BlpI GC'TnA_GC

Cuts at:   2280    2280 
   Size:       5168
 
Bme1580I G_kGCm'C

Cuts at:   1272    2793    2886    3942    1272 
   Size:       1521      93    1056    2498

  Fragments arranged by size: 

                2498    1521    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2380    2461    2796    4940    2380 
   Size:         81     335    2144    2608

  Fragments arranged by size: 

                2608    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3535    3535 
   Size:       5168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    860    1206    1480     860 
   Size:        346     274    4548

  Fragments arranged by size: 

                4548     346     274
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2344    3223    3715    4013    2344 
   Size:        879     492     298    3499

  Fragments arranged by size: 

                3499     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    706    4872     706 
   Size:       4166    1002
 
BsaAI yAC'GTr

Cuts at:    282    2160    2521     282 
   Size:       1878     361    2929

  Fragments arranged by size: 

                2929    1878     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2682    4719       6 
   Size:       2676    2037     455

  Fragments arranged by size: 

                2676    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    328    2599    2847    3549    5045     328 
   Size:       2271     248     702    1496     451

  Fragments arranged by size: 

                2271    1496     702     451     248
 
BsaWI w'CCGG_w

Cuts at:    854    2357    2878    3556    3830    3977    4388     854 
   Size:       1503     521     678     274     147     411    1634

  Fragments arranged by size: 

                1634    1503     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1370    1957    2014    2017    1370 
   Size:        587      57       3    4521

  Fragments arranged by size: 

                4521     587      57       3
 
BseYI C'CCAG_C

Cuts at:    169    1265    1763    1767    2593    3351    3928    4424     169 
   Size:       1096     498       4     826     758     577     496     913

  Fragments arranged by size: 

                1096     913     826     758     577     498     496       4
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    253     294     923    1053     253 
   Size:         41     629     130    4368

  Fragments arranged by size: 

                4368     629     130      41
 
BsiEI CG_ry'CG

Cuts at:    104    2710    2756    3964     104 
   Size:       2606      46    1208    1308

  Fragments arranged by size: 

                2606    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2182    2960    3150    3942    2182 
   Size:        778     190     792    3408

  Fragments arranged by size: 

                3408     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    244     244 
   Size:       5168
 
Bsp1286I G_dGCh'C

Cuts at:   1272    2182    2793    2886    2960    3150    3212    3942    1272 
   Size:        910     611      93      74     190      62     730    2498

  Fragments arranged by size: 

                2498     910     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   4388    4388 
   Size:       5168
 
BspHI T'CATG_A

Cuts at:   4344    4344 
   Size:       5168
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    316     864    2218    2432    2734    3115     316 
   Size:        548    1354     214     302     381    2369

  Fragments arranged by size: 

                2369    1354     548     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1378    3460    3514    4998    5163      50 
   Size:       1328    2082      54    1484     165      55

  Fragments arranged by size: 

                2082    1484    1328     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1463    3080    1463 
   Size:       1617    3551
 
BsrFI r'CCGG_y

Cuts at:    263     854    3166    3347    3556     263 
   Size:        591    2312     181     209    1875

  Fragments arranged by size: 

                2312    1875     591     209     181
 
BssSI C'ACGA_G

Cuts at:   3439    3797    3439 
   Size:        358    4810
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2545    2545 
   Size:       5168
 
BstBI TT'CG_AA

Cuts at:    110    3529     110 
   Size:       3419    1749
 
BstYI r'GATC_y

Cuts at:      1    2130    2193    2660    2683    3018    3264    4276    4362 
   Size:       2129      63     467      23     335     246    1012      86

Cuts at:   4362    4374    4720       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2129    1012     467     449     346     335     246      86
                  63      23      12
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       5168
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3118    3299    3462    3118 
   Size:        181     163    4824

  Fragments arranged by size: 

                4824     181     163
 
BtsI GCAGTG_nn'

Cuts at:    639     911    2411     639 
   Size:        272    1500    3396

  Fragments arranged by size: 

                3396    1500     272
 
DraI TTT'AAA

Cuts at:   1217    2167    1217 
   Size:        950    4218
 
DrdI GACnn_nn'nnGTC

Cuts at:    508    2874    3732     508 
   Size:       2366     858    1944

  Fragments arranged by size: 

                2366    1944     858
 
EaeI y'GGCC_r

Cuts at:    254    2753    2927    3318    3345    4690     254 
   Size:       2499     174     391      27    1345     732

  Fragments arranged by size: 

                2499    1345     732     391     174      27
 
EagI C'GGCC_G

Cuts at:   2753    2753 
   Size:       5168
 
EarI CTCTTCn'nnn_

Cuts at:    965    1922    1934    2030    3191    3401     965 
   Size:        957      12      96    1161     210    2732

  Fragments arranged by size: 

                2732    1161     957     210      96      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3376    3686    3832    4978    3376 
   Size:        310     146    1146    3566

  Fragments arranged by size: 

                3566    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2180    2180 
   Size:       5168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    860    1206    1343    1480    1958    1997    2993    3425    4172 
   Size:        346     137     137     478      39     996     432     747

Cuts at:   4172    4440     860 
   Size:        268    1588

  Fragments arranged by size: 

                1588     996     747     478     432     346     268     137
                 137      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1094    1094 
   Size:       5168
 
EcoO109I rG'GnC_Cy

Cuts at:    174    1269    2307     174 
   Size:       1095    1038    3035

  Fragments arranged by size: 

                3035    1095    1038
 
EcoRI G'AATT_C

Cuts at:   1317    2172    1317 
   Size:        855    4313
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1033    3187    1033 
   Size:       2154    3014
 
FspI TGC'GCA

Cuts at:   2949    2949 
   Size:       5168
 
HaeII r_GCGC'y

Cuts at:    331    2602    2850    3872    4851     331 
   Size:       2271     248    1022     979     648

  Fragments arranged by size: 

                2271    1022     979     648     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1144    1176    1338    1370    3010    3040    3042    3072    1144 
   Size:         32     162      32    1640      30       2      30    3240

  Fragments arranged by size: 

                3240    1640     162      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    513    1109    1718    2207     513 
   Size:        596     609     489    3474

  Fragments arranged by size: 

                3474     609     596     489
 
HindIII A'AGCT_T

Cuts at:   1837    1837 
   Size:       5168
 
HpaI GTT'AAC

Cuts at:   1718    1718 
   Size:       5168
 
KasI G'GCGC_C

Cuts at:    327    2598    2846     327 
   Size:       2271     248    2649

  Fragments arranged by size: 

                2649    2271     248
 
KpnI G_GTAC'C

Cuts at:    308    1762    2188     308 
   Size:       1454     426    3288

  Fragments arranged by size: 

                3288    1454     426
 
MluI A'CGCG_T

Cuts at:   4601    4601 
   Size:       5168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3839    4023    3839 
   Size:        184    4984
 
MscI TGG'CCA

Cuts at:   2929    2929 
   Size:       5168
 
MslI CAynn'nnrTG

Cuts at:    299    1829    3157    3284     299 
   Size:       1530    1328     127    2183

  Fragments arranged by size: 

                2183    1530    1328     127
 
MspA1I CmG'CkG

Cuts at:    682    1767    2279    2593    2953    3966    4211     682 
   Size:       1085     512     314     360    1013     245    1639

  Fragments arranged by size: 

                1639    1085    1013     512     360     314     245
 
NaeI GCC'GGC

Cuts at:   3349    3349 
   Size:       5168
 
NarI GG'CG_CC

Cuts at:    328    2599    2847     328 
   Size:       2271     248    2649

  Fragments arranged by size: 

                2649    2271     248
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       5168
 
NdeI CA'TA_TG

Cuts at:   1235    1235 
   Size:       5168
 
NgoMIV G'CCGG_C

Cuts at:   3347    3347 
   Size:       5168
 
NsiI A_TGCA'T

Cuts at:   1731    1731 
   Size:       5168
 
NspI r_CATG'y

Cuts at:    599     748    2221    2721    3628     599 
   Size:        149    1473     500     907    2139

  Fragments arranged by size: 

                2139    1473     907     500     149
 
PasI CC'CwG_GG

Cuts at:   1527    1527 
   Size:       5168
 
PciI A'CATG_T

Cuts at:   3624    3624 
   Size:       5168
 
PflMI CCAn_nnn'nTGG

Cuts at:   1788    1788 
   Size:       5168
 
PmeI GTTT'AAAC

Cuts at:   2167    2167 
   Size:       5168
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4307    4339    4307 
   Size:         32    5136
 
PpuMI rG'GwC_Cy

Cuts at:    174     174 
   Size:       5168
 
PsiI TTA'TAA

Cuts at:   1594    1594 
   Size:       5168
 
PspOMI G'GGCC_C

Cuts at:   1268    1268 
   Size:       5168
 
PstI C_TGCA'G

Cuts at:    344    2215    2900     344 
   Size:       1871     685    2612

  Fragments arranged by size: 

                2612    1871     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5168
 
PvuII CAG'CTG

Cuts at:    682    1767    2593    2953     682 
   Size:       1085     826     360    2897

  Fragments arranged by size: 

                2897    1085     826     360
 
RsrII CG'GwC_CG

Cuts at:   3363    3363 
   Size:       5168
 
SacI G_AGCT'C

Cuts at:   2182    2182 
   Size:       5168
 
SalI G'TCGA_C

Cuts at:   2205    2205 
   Size:       5168
 
SanDI GG'GwC_CC

Cuts at:    174     174 
   Size:       5168
 
SapI GCTCTTCn'nnn_

Cuts at:    965    3191    3401     965 
   Size:       2226     210    2732

  Fragments arranged by size: 

                2732    2226     210
 
SbfI CC_TGCA'GG

Cuts at:   2215    2215 
   Size:       5168
 
ScaI AGT'ACT

Cuts at:   1568    4685    1568 
   Size:       3117    2051
 
SexAI A'CCwGG_T

Cuts at:   1754    1754 
   Size:       5168
 
SfcI C'TryA_G

Cuts at:     33     340     386     726    2211    2896    3889    4080      33 
   Size:        307      46     340    1485     685     993     191    1121

  Fragments arranged by size: 

                1485    1121     993     685     340     307     191      46
            
 
SfoI GGC'GCC

Cuts at:    329    2600    2848     329 
   Size:       2271     248    2649

  Fragments arranged by size: 

                2649    2271     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5168
 
SmaI CCC'GGG

Cuts at:   2190    2190 
   Size:       5168
 
SmlI C'TyrA_G

Cuts at:   2323    3238    3730    3992    2323 
   Size:        915     492     262    3499

  Fragments arranged by size: 

                3499     915     492     262
 
SnaBI TAC'GTA

Cuts at:   2160    2160 
   Size:       5168
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5168
 
SphI G_CATG'C

Cuts at:   2221    2721    2221 
   Size:        500    4668
 
StuI AGG'CCT

Cuts at:    205     205 
   Size:       5168
 
StyI C'CwwG_G

Cuts at:    125     347    2302    2656     125 
   Size:        222    1955     354    2637

  Fragments arranged by size: 

                2637    1955     354     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2751    2884    3343    3552    2751 
   Size:        133     459     209    4367

  Fragments arranged by size: 

                4367     459     209     133
 
TatI w'GTAC_w

Cuts at:    846    1566    4683     846 
   Size:        720    3117    1331

  Fragments arranged by size: 

                3117    1331     720
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4685    4717    4685 
   Size:         32    5136
 
Tth111I GACn'n_nGTC

Cuts at:   2965    2965 
   Size:       5168
 
XbaI T'CTAG_A

Cuts at:   2199    2199 
   Size:       5168
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    808    2663     808 
   Size:       1855    3313
 
XmaI C'CCGG_G

Cuts at:   2188    2188 
   Size:       5168
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI     PsiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AlfI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI
     BsmI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI     MfeI     NheI     NotI
     NruI     PacI     PfoI     PmlI    PshAI    PspXI     PsrI    SacII
     SfiI    SgrAI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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