Restriction map for
pF1KSDA0016
(Circular) (Six-base) MAPSORT of: pF1KSDA0016.seq Check: 6681 from: 1 to: 3572
pF1KSDA0016 3572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 395 610 395
Size: 215 3357
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 566 1397 1829 2576 2844 335
Size: 231 831 432 747 268 1063
Fragments arranged by size:
1063 831 747 432 268 231
AflIII A'CryG_T
Cuts at: 924 2028 3005 924
Size: 1104 977 1491
Fragments arranged by size:
1491 1104 977
AgeI A'CCGG_T
Cuts at: 1960 1960
Size: 3572
AlwNI CAG_nnn'CTG
Cuts at: 444 877 2444 444
Size: 433 1567 1572
Fragments arranged by size:
1572 1567 433
ApaI G_GGCC'C
Cuts at: 171 171
Size: 3572
ApaLI G'TGCA_C
Cuts at: 2342 2342
Size: 3572
ApoI r'AATT_y
Cuts at: 576 2925 3181 576
Size: 2349 256 967
Fragments arranged by size:
2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 592 592
Size: 3572
BamHI G'GATC_C
Cuts at: 597 597
Size: 3572
BanI G'GyrC_C
Cuts at: 588 1002 1250 1285 588
Size: 414 248 35 2875
Fragments arranged by size:
2875 414 248 35
BanII G_rGCy'C
Cuts at: 171 586 1616 171
Size: 415 1030 2127
Fragments arranged by size:
2127 1030 415
BbeI G_GCGC'C
Cuts at: 1006 1254 1006
Size: 248 3324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 177 211 177
Size: 34 3538
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2231 3572 1470
Size: 761 1341 1470
Fragments arranged by size:
1470 1341 761
BclI T'GATC_A
Cuts at: 1092 1092
Size: 3572
BglII A'GATC_T
Cuts at: 1087 3124 1087
Size: 2037 1535
BlpI GC'TnA_GC
Cuts at: 684 684
Size: 3572
Bme1580I G_kGCm'C
Cuts at: 171 1197 1290 2346 171
Size: 1026 93 1056 1397
Fragments arranged by size:
1397 1056 1026 93
BmrI ACTGGGnnnn_n'
Cuts at: 784 865 1200 3344 784
Size: 81 335 2144 1012
Fragments arranged by size:
2144 1012 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 748 1627 2119 2417 356
Size: 392 879 492 298 1511
Fragments arranged by size:
1511 879 492 392 298
BsaI GGTCTCn'nnnn_
Cuts at: 3276 3276
Size: 3572
BsaAI yAC'GTr
Cuts at: 564 925 564
Size: 361 3211
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 1003 1251 1953 3449 1003
Size: 248 702 1496 1126
Fragments arranged by size:
1496 1126 702 248
BsaWI w'CCGG_w
Cuts at: 761 1282 1960 2234 2381 2792 761
Size: 521 678 274 147 411 1541
Fragments arranged by size:
1541 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 280 997 1755 2332 2828 280
Size: 717 758 577 496 1024
Fragments arranged by size:
1024 758 717 577 496
BsiEI CG_ry'CG
Cuts at: 104 201 1114 1160 2368 104
Size: 97 913 46 1208 1308
Fragments arranged by size:
1308 1208 913 97 46
BsiHKAI G_wGCw'C
Cuts at: 534 586 1364 1554 2346 534
Size: 52 778 190 792 1760
Fragments arranged by size:
1760 792 778 190 52
Bsp1286I G_dGCh'C
Cuts at: 171 534 586 1197 1290 1364 1554 1616 2346
Size: 363 52 611 93 74 190 62 730
Cuts at: 2346 171
Size: 1397
Fragments arranged by size:
1397 730 611 363 190 93 74 62
52
BspEI T'CCGG_A
Cuts at: 2792 2792
Size: 3572
BspHI T'CATG_A
Cuts at: 2748 2748
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 622 836 1138 1519 438
Size: 184 214 302 381 2491
Fragments arranged by size:
2491 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 1864 1918 3402 3567 50
Size: 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1484 1484
Size: 3572
BsrFI r'CCGG_y
Cuts at: 154 1570 1751 1960 154
Size: 1416 181 209 1766
Fragments arranged by size:
1766 1416 209 181
BssSI C'ACGA_G
Cuts at: 1843 2201 1843
Size: 358 3214
BstAPI GCAn_nnn'nTGC
Cuts at: 444 949 444
Size: 505 3067
BstBI TT'CG_AA
Cuts at: 110 1933 110
Size: 1823 1749
BstYI r'GATC_y
Cuts at: 1 597 1064 1087 1422 1668 2680 2766 2778
Size: 596 467 23 335 246 1012 86 12
Cuts at: 2778 3124 1
Size: 346 449
Fragments arranged by size:
1012 596 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 3572
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 132 1522 1703 1866 132
Size: 1390 181 163 1838
Fragments arranged by size:
1838 1390 181 163
BtsI GCAGTG_nn'
Cuts at: 815 815
Size: 3572
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 1278 2136 1278
Size: 858 2714
EaeI y'GGCC_r
Cuts at: 1157 1331 1722 1749 3094 1157
Size: 174 391 27 1345 1635
Fragments arranged by size:
1635 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1157 1157
Size: 3572
EarI CTCTTCn'nnn_
Cuts at: 353 470 1595 1805 353
Size: 117 1125 210 2120
Fragments arranged by size:
2120 1125 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2090 2236 3382 1780
Size: 310 146 1146 1970
Fragments arranged by size:
1970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 566 1397 1829 2576 2844 335
Size: 231 831 432 747 268 1063
Fragments arranged by size:
1063 831 747 432 268 231
EcoO109I rG'GnC_Cy
Cuts at: 167 168 711 167
Size: 1 543 3028
Fragments arranged by size:
3028 543 1
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1591 1591
Size: 3572
FspI TGC'GCA
Cuts at: 1353 1353
Size: 3572
HaeII r_GCGC'y
Cuts at: 146 1006 1254 2276 3255 146
Size: 860 248 1022 979 463
Fragments arranged by size:
1022 979 860 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1414 1444 1446 1476 1414
Size: 30 2 30 3510
Fragments arranged by size:
3510 30 30 2
HincII GTy'rAC
Cuts at: 611 611
Size: 3572
HindIII A'AGCT_T
Cuts at: 262 262
Size: 3572
KasI G'GCGC_C
Cuts at: 1002 1250 1002
Size: 248 3324
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MfeI C'AATT_G
Cuts at: 413 413
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 115 2243 2427 115
Size: 2128 184 1260
Fragments arranged by size:
2128 1260 184
MscI TGG'CCA
Cuts at: 1333 1333
Size: 3572
MslI CAynn'nnrTG
Cuts at: 1561 1688 1561
Size: 127 3445
MspA1I CmG'CkG
Cuts at: 683 997 1357 2370 2615 683
Size: 314 360 1013 245 1640
Fragments arranged by size:
1640 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 1003 1251 1003
Size: 248 3324
NcoI C'CATG_G
Cuts at: 125 125
Size: 3572
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 625 1125 2032 625
Size: 500 907 2165
Fragments arranged by size:
2165 907 500
PciI A'CATG_T
Cuts at: 2028 2028
Size: 3572
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 3540
PspOMI G'GGCC_C
Cuts at: 167 167
Size: 3572
PstI C_TGCA'G
Cuts at: 449 619 1304 449
Size: 170 685 2717
Fragments arranged by size:
2717 685 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3572
PvuII CAG'CTG
Cuts at: 997 1357 997
Size: 360 3212
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 609 609
Size: 3572
SapI GCTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 445 615 1300 2293 2484 33
Size: 412 170 685 993 191 1121
Fragments arranged by size:
1121 993 685 412 191 170
SfoI GGC'GCC
Cuts at: 1004 1252 1004
Size: 248 3324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SgrAI Cr'CCGG_yG
Cuts at: 154 154
Size: 3572
SmaI CCC'GGG
Cuts at: 594 594
Size: 3572
SmlI C'TyrA_G
Cuts at: 371 727 1642 2134 2396 371
Size: 356 915 492 262 1547
Fragments arranged by size:
1547 915 492 356 262
SnaBI TAC'GTA
Cuts at: 564 564
Size: 3572
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 625 1125 625
Size: 500 3072
StyI C'CwwG_G
Cuts at: 125 269 706 1060 125
Size: 144 437 354 2637
Fragments arranged by size:
2637 437 354 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1288 1747 1956 1155
Size: 133 459 209 2771
Fragments arranged by size:
2771 459 209 133
TatI w'GTAC_w
Cuts at: 3087 3087
Size: 3572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 3089 3121 464
Size: 32 2593 32 915
Fragments arranged by size:
2593 915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1369 1369
Size: 3572
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1067 1067
Size: 3572
XmaI C'CCGG_G
Cuts at: 592 592
Size: 3572
XmnI GAAnn'nnTTC
Cuts at: 320 331 320
Size: 11 3561
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SrfI SspI StuI SwaI XhoI ZraI