Restriction map for
pF1KSDA0017
(Circular) (Six-base) MAPSORT of: pF1KSDA0017.seq Check: 7695 from: 1 to: 6788
pF1KSDA0017 6788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3575 3575
Size: 6788
AccI GT'mk_AC
Cuts at: 1515 3826 1515
Size: 2311 4477
Acc65I G'GTAC_C
Cuts at: 2201 3804 2201
Size: 1603 5185
AclI AA'CG_TT
Cuts at: 6628 6628
Size: 6788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1820 3728 4613 5045 5792 6060 1820
Size: 1908 885 432 747 268 2548
Fragments arranged by size:
2548 1908 885 747 432 268
AflIII A'CryG_T
Cuts at: 4140 5244 6221 4140
Size: 1104 977 4707
Fragments arranged by size:
4707 1104 977
AgeI A'CCGG_T
Cuts at: 5176 5176
Size: 6788
AhdI GACnn_n'nnGTC
Cuts at: 1511 1511
Size: 6788
AleI CACnn'nnGTG
Cuts at: 622 1573 622
Size: 951 5837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3529 3529
Size: 6788
AlwNI CAG_nnn'CTG
Cuts at: 841 4093 5660 841
Size: 3252 1567 1969
Fragments arranged by size:
3252 1969 1567
ApaLI G'TGCA_C
Cuts at: 5558 5558
Size: 6788
ApoI r'AATT_y
Cuts at: 1180 2344 2485 3792 6141 6397 1180
Size: 1164 141 1307 2349 256 1571
Fragments arranged by size:
2349 1571 1307 1164 256 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 6788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6788
AvaI C'yCGr_G
Cuts at: 2377 3748 3808 2377
Size: 1371 60 5357
Fragments arranged by size:
5357 1371 60
BamHI G'GATC_C
Cuts at: 495 1830 1983 3101 3330 3813 495
Size: 1335 153 1118 229 483 3470
Fragments arranged by size:
3470 1335 1118 483 229 153
BanI G'GyrC_C
Cuts at: 343 2201 3804 4218 4466 4501 343
Size: 1858 1603 414 248 35 2630
Fragments arranged by size:
2630 1858 1603 414 248 35
BanII G_rGCy'C
Cuts at: 2101 2688 3802 4832 2101
Size: 587 1114 1030 4057
Fragments arranged by size:
4057 1114 1030 587
BbeI G_GCGC'C
Cuts at: 4222 4470 4222
Size: 248 6540
BbsI GAAGACnn'nnnn_
Cuts at: 1741 2436 2834 3448 1741
Size: 695 398 614 5081
Fragments arranged by size:
5081 695 614 398
BbvCI CC'TCA_GC
Cuts at: 2149 3607 2149
Size: 1458 5330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1912 1946 1912
Size: 34 6754
BciVI GTATCCnnnnn_n'
Cuts at: 4686 5447 6788 4686
Size: 761 1341 4686
Fragments arranged by size:
4686 1341 761
BclI T'GATC_A
Cuts at: 3398 4308 3398
Size: 910 5878
BglII A'GATC_T
Cuts at: 233 4303 6340 233
Size: 4070 2037 681
Fragments arranged by size:
4070 2037 681
BlpI GC'TnA_GC
Cuts at: 3900 3900
Size: 6788
Bme1580I G_kGCm'C
Cuts at: 4413 4506 5562 4413
Size: 93 1056 5639
Fragments arranged by size:
5639 1056 93
BmgBI CAC'GTC
Cuts at: 3524 3524
Size: 6788
BmrI ACTGGGnnnn_n'
Cuts at: 2183 4000 4081 4416 6560 2183
Size: 1817 81 335 2144 2411
Fragments arranged by size:
2411 2144 1817 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2115 5155 2115
Size: 3040 3748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 1937 2956 3479 3515 863
Size: 1074 1019 523 36 4136
Fragments arranged by size:
4136 1074 1019 523 36
Bpu10I CC'TnA_GC
Cuts at: 2149 3607 2149
Size: 1458 5330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 3964 4843 5335 5633 1471
Size: 2493 879 492 298 2626
Fragments arranged by size:
2626 2493 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1934 3647 6492 1934
Size: 1713 2845 2230
Fragments arranged by size:
2845 2230 1713
BsaAI yAC'GTr
Cuts at: 3780 4141 3780
Size: 361 6427
BsaBI GATnn'nnATC
Cuts at: 6 3329 4302 6339 6
Size: 3323 973 2037 455
Fragments arranged by size:
3323 2037 973 455
BsaHI Gr'CG_yC
Cuts at: 4219 4467 5169 6665 4219
Size: 248 702 1496 4342
Fragments arranged by size:
4342 1496 702 248
BsaWI w'CCGG_w
Cuts at: 311 3758 3977 4498 5176 5450 5597 6008 311
Size: 3447 219 521 678 274 147 411 1091
Fragments arranged by size:
3447 1091 678 521 411 274 219 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 632 662 1580 1610 1799 1829 632
Size: 30 918 30 189 30 5591
Fragments arranged by size:
5591 918 189 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1820 1820
Size: 6788
BseYI C'CCAG_C
Cuts at: 737 2025 2030 2085 3259 4213 4971 5548 6044
Size: 1288 5 55 1174 954 758 577 496
Cuts at: 6044 737
Size: 1481
Fragments arranged by size:
1481 1288 1174 954 758 577 496 55
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 1787 2826 1696
Size: 91 1039 5658
Fragments arranged by size:
5658 1039 91
BsiEI CG_ry'CG
Cuts at: 104 4330 4376 5584 104
Size: 4226 46 1208 1308
Fragments arranged by size:
4226 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2688 3802 4580 4770 5562 2688
Size: 1114 778 190 792 3914
Fragments arranged by size:
3914 1114 792 778 190
BsmI GAATG_Cn'
Cuts at: 1569 1694 2246 2647 1569
Size: 125 552 401 5710
Fragments arranged by size:
5710 552 401 125
BsmBI CGTCTCn'nnnn_
Cuts at: 3415 3708 3415
Size: 293 6495
Bsp1286I G_dGCh'C
Cuts at: 2101 2688 3802 4413 4506 4580 4770 4832 5562
Size: 587 1114 611 93 74 190 62 730
Cuts at: 5562 2101
Size: 3327
Fragments arranged by size:
3327 1114 730 611 587 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3758 6008 3758
Size: 2250 4538
BspHI T'CATG_A
Cuts at: 3401 5964 3401
Size: 2563 4225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1668 2798 3575 3838 4052 4354 4735 1668
Size: 1130 777 263 214 302 381 3721
Fragments arranged by size:
3721 1130 777 381 302 263 214
BsrBI CCG'CTC
Cuts at: 50 2279 2619 5080 5134 6618 6783 50
Size: 2229 340 2461 54 1484 165 55
Fragments arranged by size:
2461 2229 1484 340 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2706 4700 2706
Size: 1994 4794
BsrFI r'CCGG_y
Cuts at: 4786 4967 5176 4786
Size: 181 209 6398
Fragments arranged by size:
6398 209 181
BsrGI T'GTAC_A
Cuts at: 134 134
Size: 6788
BssSI C'ACGA_G
Cuts at: 5059 5417 5059
Size: 358 6430
BstAPI GCAn_nnn'nTGC
Cuts at: 4165 4165
Size: 6788
BstBI TT'CG_AA
Cuts at: 110 5149 110
Size: 5039 1749
BstEII G'GTnAC_C
Cuts at: 910 1486 910
Size: 576 6212
BstXI CCAn_nnnn'nTGG
Cuts at: 1260 3112 3240 1260
Size: 1852 128 4808
Fragments arranged by size:
4808 1852 128
BstYI r'GATC_y
Cuts at: 1 233 495 1097 1830 1983 3101 3330 3813
Size: 232 262 602 733 153 1118 229 483
Cuts at: 3813 4280 4303 4638 4884 5896 5982 5994 6340
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6340 1
Size: 449
Fragments arranged by size:
1118 1012 733 602 483 467 449 346
335 262 246 232 229 153 86 23
12
BtgI C'CryG_G
Cuts at: 225 3360 3677 225
Size: 3135 317 3336
Fragments arranged by size:
3336 3135 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4738 4919 5082 4738
Size: 181 163 6444
Fragments arranged by size:
6444 181 163
BtsI GCAGTG_nn'
Cuts at: 375 1775 2464 2713 4031 375
Size: 1400 689 249 1318 3132
Fragments arranged by size:
3132 1400 1318 689 249
ClaI AT'CG_AT
Cuts at: 1015 1015
Size: 6788
DraI TTT'AAA
Cuts at: 776 3787 776
Size: 3011 3777
DrdI GACnn_nn'nnGTC
Cuts at: 1720 1963 3429 4494 5352 1720
Size: 243 1466 1065 858 3156
Fragments arranged by size:
3156 1466 1065 858 243
EaeI y'GGCC_r
Cuts at: 477 1370 1901 2264 2390 2627 2852 4373 4547
Size: 893 531 363 126 237 225 1521 174
Cuts at: 4547 4938 4965 6310 477
Size: 391 27 1345 955
Fragments arranged by size:
1521 1345 955 893 531 391 363 237
225 174 126 27
EagI C'GGCC_G
Cuts at: 4373 4373
Size: 6788
EarI CTCTTCn'nnn_
Cuts at: 2233 2957 4811 5021 2233
Size: 724 1854 210 4000
Fragments arranged by size:
4000 1854 724 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 963 4996 5306 5452 6598 963
Size: 4033 310 146 1146 1153
Fragments arranged by size:
4033 1153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2686 3800 2686
Size: 1114 5674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 1820 1937 2956 3479 3515 3728 4613 5045
Size: 957 117 1019 523 36 213 885 432
Cuts at: 5045 5792 6060 863
Size: 747 268 1591
Fragments arranged by size:
1591 1019 957 885 747 523 432 268
213 117 36
EcoNI CCTnn'n_nnAGG
Cuts at: 971 1407 971
Size: 436 6352
EcoO109I rG'GnC_Cy
Cuts at: 1414 2210 2859 2967 3377 3927 1414
Size: 796 649 108 410 550 4275
Fragments arranged by size:
4275 796 649 550 410 108
EcoRI G'AATT_C
Cuts at: 3792 3792
Size: 6788
EcoRV GAT'ATC
Cuts at: 924 1053 3756 924
Size: 129 2703 3956
Fragments arranged by size:
3956 2703 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4807 4807
Size: 6788
FspI TGC'GCA
Cuts at: 4569 4569
Size: 6788
HaeII r_GCGC'y
Cuts at: 4222 4470 5492 6471 4222
Size: 248 1022 979 4539
Fragments arranged by size:
4539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 857 889 1231 1263 1321 1350 1353
Size: 32 193 32 342 32 58 29 3
Cuts at: 1353 1382 1497 1529 1799 1831 1912 1944 4630
Size: 29 115 32 270 32 81 32 2686
Cuts at: 4630 4660 4662 4692 632
Size: 30 2 30 2728
Fragments arranged by size:
2728 2686 342 270 193 115 81 58
32 32 32 32 32 32 30 30
29 29 3 2
HincII GTy'rAC
Cuts at: 1516 3827 1516
Size: 2311 4477
HindIII A'AGCT_T
Cuts at: 2911 2911
Size: 6788
KasI G'GCGC_C
Cuts at: 4218 4466 4218
Size: 248 6540
KpnI G_GTAC'C
Cuts at: 2205 3808 2205
Size: 1603 5185
MfeI C'AATT_G
Cuts at: 1754 1754
Size: 6788
MluI A'CGCG_T
Cuts at: 6221 6221
Size: 6788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 630 736 1307 2840 5459 5643 618
Size: 12 106 571 1533 2619 184 1763
Fragments arranged by size:
2619 1763 1533 571 184 106 12
MscI TGG'CCA
Cuts at: 479 1372 1903 2266 2392 2854 4549 479
Size: 893 531 363 126 462 1695 2718
Fragments arranged by size:
2718 1695 893 531 462 363 126
MslI CAynn'nnrTG
Cuts at: 622 1144 1549 1573 3286 3511 4777 4904 622
Size: 522 405 24 1713 225 1266 127 2506
Fragments arranged by size:
2506 1713 1266 522 405 225 127 24
MspA1I CmG'CkG
Cuts at: 929 1845 1926 2769 3899 4213 4573 5586 5831
Size: 916 81 843 1130 314 360 1013 245
Cuts at: 5831 929
Size: 1886
Fragments arranged by size:
1886 1130 1013 916 843 360 314 245
81
NaeI GCC'GGC
Cuts at: 4969 4969
Size: 6788
NarI GG'CG_CC
Cuts at: 4219 4467 4219
Size: 248 6540
NcoI C'CATG_G
Cuts at: 3677 3677
Size: 6788
NdeI CA'TA_TG
Cuts at: 3283 3283
Size: 6788
NgoMIV G'CCGG_C
Cuts at: 4967 4967
Size: 6788
NspI r_CATG'y
Cuts at: 2016 3556 3841 4341 5248 2016
Size: 1540 285 500 907 3556
Fragments arranged by size:
3556 1540 907 500 285
PciI A'CATG_T
Cuts at: 5244 5244
Size: 6788
PflMI CCAn_nnn'nTGG
Cuts at: 3555 3555
Size: 6788
PfoI T'CCnGG_A
Cuts at: 840 3239 840
Size: 2399 4389
PmeI GTTT'AAAC
Cuts at: 3787 3787
Size: 6788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5927 5959 5927
Size: 32 6756
PpuMI rG'GwC_Cy
Cuts at: 1414 2210 2859 2967 1414
Size: 796 649 108 5235
Fragments arranged by size:
5235 796 649 108
PshAI GACnn'nnGTC
Cuts at: 377 377
Size: 6788
PspXI vC'TCGA_Gb
Cuts at: 3748 3748
Size: 6788
PstI C_TGCA'G
Cuts at: 573 2471 3835 4520 573
Size: 1898 1364 685 2841
Fragments arranged by size:
2841 1898 1364 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6788
PvuII CAG'CTG
Cuts at: 1845 2769 4213 4573 1845
Size: 924 1444 360 4060
Fragments arranged by size:
4060 1444 924 360
RsrII CG'GwC_CG
Cuts at: 4983 4983
Size: 6788
SacI G_AGCT'C
Cuts at: 2688 3802 2688
Size: 1114 5674
SalI G'TCGA_C
Cuts at: 1514 3825 1514
Size: 2311 4477
SanDI GG'GwC_CC
Cuts at: 1414 2210 1414
Size: 796 5992
SapI GCTCTTCn'nnn_
Cuts at: 2233 4811 5021 2233
Size: 2578 210 4000
Fragments arranged by size:
4000 2578 210
SbfI CC_TGCA'GG
Cuts at: 3835 3835
Size: 6788
ScaI AGT'ACT
Cuts at: 869 6305 869
Size: 5436 1352
SexAI A'CCwGG_T
Cuts at: 1482 1482
Size: 6788
SfcI C'TryA_G
Cuts at: 33 569 1595 2131 2467 3227 3335 3831 4516
Size: 536 1026 536 336 760 108 496 685
Cuts at: 4516 5509 5700 33
Size: 993 191 1121
Fragments arranged by size:
1121 1026 993 760 685 536 536 496
336 191 108
SfoI GGC'GCC
Cuts at: 4220 4468 4220
Size: 248 6540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6788
SmaI CCC'GGG
Cuts at: 3810 3810
Size: 6788
SmlI C'TyrA_G
Cuts at: 1450 3748 3943 4858 5350 5612 1450
Size: 2298 195 915 492 262 2626
Fragments arranged by size:
2626 2298 915 492 262 195
SnaBI TAC'GTA
Cuts at: 3780 3780
Size: 6788
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6788
SphI G_CATG'C
Cuts at: 2016 3841 4341 2016
Size: 1825 500 4463
Fragments arranged by size:
4463 1825 500
SspI AAT'ATT
Cuts at: 1111 2124 1111
Size: 1013 5775
StyI C'CwwG_G
Cuts at: 768 1670 2690 2855 3677 3922 4276 768
Size: 902 1020 165 822 245 354 3280
Fragments arranged by size:
3280 1020 902 822 354 245 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2050 2077 2493 2836 3734 4371 4504 4963 5172
Size: 27 416 343 898 637 133 459 209
Cuts at: 5172 2050
Size: 3666
Fragments arranged by size:
3666 898 637 459 416 343 209 133
27
TatI w'GTAC_w
Cuts at: 134 273 867 1853 2471 6303 134
Size: 139 594 986 618 3832 619
Fragments arranged by size:
3832 986 619 618 594 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1439 1471 6305 6337 1439
Size: 32 4834 32 1890
Fragments arranged by size:
4834 1890 32 32
Tth111I GACn'n_nGTC
Cuts at: 1641 2167 2761 4585 1641
Size: 526 594 1824 3844
Fragments arranged by size:
3844 1824 594 526
XbaI T'CTAG_A
Cuts at: 3819 3819
Size: 6788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 442 4283 442
Size: 3841 2947
XhoI C'TCGA_G
Cuts at: 3748 3748
Size: 6788
XmaI C'CCGG_G
Cuts at: 3808 3808
Size: 6788
XmnI GAAnn'nnTTC
Cuts at: 189 2227 189
Size: 2038 4750
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BfrBI BglI BmtI BsiWI BssHII BstZ17I Bsu36I CspCI
DraIII FseI FspAI HpaI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PspOMI PsrI SacII SfiI
SgrAI SrfI StuI SwaI ZraI