Restriction map for
pF1KSDA0019
(Circular) (Six-base) MAPSORT of: pF1KSDA0019.seq Check: 6047 from: 1 to: 5621
pF1KSDA0019 5621 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 1604 1604
Size: 5621
AccI GT'mk_AC
Cuts at: 2659 2659
Size: 5621
Acc65I G'GTAC_C
Cuts at: 2637 2637
Size: 5621
AclI AA'CG_TT
Cuts at: 5461 5461
Size: 5621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1240 3446 3878 4625 4893 1135
Size: 105 2206 432 747 268 1863
Fragments arranged by size:
2206 1863 747 432 268 105
AflII C'TTAA_G
Cuts at: 871 871
Size: 5621
AflIII A'CryG_T
Cuts at: 2973 4077 5054 2973
Size: 1104 977 3540
Fragments arranged by size:
3540 1104 977
AgeI A'CCGG_T
Cuts at: 4009 4009
Size: 5621
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 992 1024 992
Size: 32 5589
AlwNI CAG_nnn'CTG
Cuts at: 1201 1690 2467 2926 4493 1201
Size: 489 777 459 1567 2329
Fragments arranged by size:
2329 1567 777 489 459
ApaI G_GGCC'C
Cuts at: 459 750 2546 459
Size: 291 1796 3534
Fragments arranged by size:
3534 1796 291
ApaLI G'TGCA_C
Cuts at: 4391 4391
Size: 5621
ApoI r'AATT_y
Cuts at: 129 413 856 1087 2625 4974 5230 129
Size: 284 443 231 1538 2349 256 520
Fragments arranged by size:
2349 1538 520 443 284 256 231
AseI AT'TA_AT
Cuts at: 20 20
Size: 5621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
AvaI C'yCGr_G
Cuts at: 1399 1881 1994 2384 2641 1399
Size: 482 113 390 257 4379
Fragments arranged by size:
4379 482 390 257 113
BamHI G'GATC_C
Cuts at: 2646 2646
Size: 5621
BanI G'GyrC_C
Cuts at: 1344 2637 3051 3299 3334 1344
Size: 1293 414 248 35 3631
Fragments arranged by size:
3631 1293 414 248 35
BanII G_rGCy'C
Cuts at: 293 459 750 1315 1762 1855 2546 2635 3665
Size: 166 291 565 447 93 691 89 1030
Cuts at: 3665 293
Size: 2249
Fragments arranged by size:
2249 1030 691 565 447 291 166 93
89
BbeI G_GCGC'C
Cuts at: 1348 3055 3303 1348
Size: 1707 248 3666
Fragments arranged by size:
3666 1707 248
BbvCI CC'TCA_GC
Cuts at: 1742 1742
Size: 5621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 718 752 718
Size: 34 5587
BciVI GTATCCnnnnn_n'
Cuts at: 1932 3519 4280 5621 1932
Size: 1587 761 1341 1932
Fragments arranged by size:
1932 1587 1341 761
BclI T'GATC_A
Cuts at: 2291 3141 2291
Size: 850 4771
BfrBI ATG'CAT
Cuts at: 786 786
Size: 5621
BglI GCCn_nnn'nGGC
Cuts at: 745 1236 745
Size: 491 5130
BglII A'GATC_T
Cuts at: 3136 5173 3136
Size: 2037 3584
BlpI GC'TnA_GC
Cuts at: 1895 2733 1895
Size: 838 4783
Bme1580I G_kGCm'C
Cuts at: 459 750 1580 2546 3246 3339 4395 459
Size: 291 830 966 700 93 1056 1685
Fragments arranged by size:
1685 1056 966 830 700 291 93
BmrI ACTGGGnnnn_n'
Cuts at: 2833 2914 3249 5393 2833
Size: 81 335 2144 3061
Fragments arranged by size:
3061 2144 335 81
BmtI G_CTAG'C
Cuts at: 2005 2396 2005
Size: 391 5230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3988 3988
Size: 5621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 2537 418
Size: 2119 3502
Bpu10I CC'TnA_GC
Cuts at: 1742 1742
Size: 5621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 840 875 1299 2797 3676 4168 4466 487
Size: 353 35 424 1498 879 492 298 1642
Fragments arranged by size:
1642 1498 879 492 424 353 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 1093 2124 5325 1093
Size: 1031 3201 1389
Fragments arranged by size:
3201 1389 1031
BsaAI yAC'GTr
Cuts at: 2613 2974 2613
Size: 361 5260
BsaBI GATnn'nnATC
Cuts at: 6 1217 3135 5172 6
Size: 1211 1918 2037 455
Fragments arranged by size:
2037 1918 1211 455
BsaHI Gr'CG_yC
Cuts at: 1268 1345 1396 1601 3052 3300 4002 5498 1268
Size: 77 51 205 1451 248 702 1496 1391
Fragments arranged by size:
1496 1451 1391 702 248 205 77 51
BsaWI w'CCGG_w
Cuts at: 2166 2810 3331 4009 4283 4430 4841 2166
Size: 644 521 678 274 147 411 2946
Fragments arranged by size:
2946 678 644 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 992 1022 1351 1381 992
Size: 30 329 30 5232
Fragments arranged by size:
5232 329 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 301 703 1308 1450 1453 1837 298
Size: 3 402 605 142 3 384 4082
Fragments arranged by size:
4082 605 402 384 142 3 3
BseYI C'CCAG_C
Cuts at: 1876 1976 2245 3046 3804 4381 4877 1876
Size: 100 269 801 758 577 496 2620
Fragments arranged by size:
2620 801 758 577 496 269 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 230
Size: 5621
BsiEI CG_ry'CG
Cuts at: 104 1309 2504 3163 3209 4417 104
Size: 1205 1195 659 46 1208 1308
Fragments arranged by size:
1308 1208 1205 1195 659 46
BsiHKAI G_wGCw'C
Cuts at: 293 528 1367 1410 2635 3413 3603 4395 293
Size: 235 839 43 1225 778 190 792 1519
Fragments arranged by size:
1519 1225 839 792 778 235 190 43
BsmBI CGTCTCn'nnnn_
Cuts at: 1608 2356 1608
Size: 748 4873
Bsp1286I G_dGCh'C
Cuts at: 293 459 528 750 1315 1367 1410 1580 1762
Size: 166 69 222 565 52 43 170 182
Cuts at: 1762 1855 2546 2635 3246 3339 3413 3603 3665
Size: 93 691 89 611 93 74 190 62
Cuts at: 3665 4395 293
Size: 730 1519
Fragments arranged by size:
1519 730 691 611 565 222 190 182
170 166 93 93 89 74 69 62
52 43
BspEI T'CCGG_A
Cuts at: 2166 4841 2166
Size: 2675 2946
BspHI T'CATG_A
Cuts at: 837 4797 837
Size: 3960 1661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2671 2885 3187 3568 2671
Size: 214 302 381 4724
Fragments arranged by size:
4724 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1340 3913 3967 5451 5616 50
Size: 1290 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 1290 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3533 3533
Size: 5621
BsrFI r'CCGG_y
Cuts at: 1304 2499 3619 3800 4009 1304
Size: 1195 1120 181 209 2916
Fragments arranged by size:
2916 1195 1120 209 181
BssSI C'ACGA_G
Cuts at: 1360 3892 4250 1360
Size: 2532 358 2731
Fragments arranged by size:
2731 2532 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2512 2998 2512
Size: 486 5135
BstBI TT'CG_AA
Cuts at: 110 3982 110
Size: 3872 1749
BstEII G'GTnAC_C
Cuts at: 2270 2270
Size: 5621
BstXI CCAn_nnnn'nTGG
Cuts at: 1984 1984
Size: 5621
BstYI r'GATC_y
Cuts at: 1 470 2646 3113 3136 3471 3717 4729 4815
Size: 469 2176 467 23 335 246 1012 86
Cuts at: 4815 4827 5173 1
Size: 12 346 449
Fragments arranged by size:
2176 1012 469 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1070 1634 1817 2040 2397 2448 1070
Size: 564 183 223 357 51 4243
Fragments arranged by size:
4243 564 357 223 183 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1754 3571 3752 3915 1754
Size: 1817 181 163 3460
Fragments arranged by size:
3460 1817 181 163
BtsI GCAGTG_nn'
Cuts at: 1763 2864 1763
Size: 1101 4520
DraI TTT'AAA
Cuts at: 1941 2620 1941
Size: 679 4942
DrdI GACnn_nn'nnGTC
Cuts at: 565 3327 4185 565
Size: 2762 858 2001
Fragments arranged by size:
2762 2001 858
EaeI y'GGCC_r
Cuts at: 1306 1319 1717 2501 3206 3380 3771 3798 5143
Size: 13 398 784 705 174 391 27 1345
Cuts at: 5143 1306
Size: 1784
Fragments arranged by size:
1784 1345 784 705 398 391 174 27
13
EagI C'GGCC_G
Cuts at: 1306 2501 3206 1306
Size: 1195 705 3721
Fragments arranged by size:
3721 1195 705
EarI CTCTTCn'nnn_
Cuts at: 766 3644 3854 766
Size: 2878 210 2533
Fragments arranged by size:
2878 2533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3829 4139 4285 5431 3829
Size: 310 146 1146 4019
Fragments arranged by size:
4019 1146 310 146
EcoICRI GAG'CTC
Cuts at: 291 2633 291
Size: 2342 3279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1135 1240 2537 3446 3878 4625 4893 418
Size: 717 105 1297 909 432 747 268 1146
Fragments arranged by size:
1297 1146 909 747 717 432 268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 1108 1108
Size: 5621
EcoO109I rG'GnC_Cy
Cuts at: 456 498 747 769 1687 1731 2542 2543 2760
Size: 42 249 22 918 44 811 1 217
Cuts at: 2760 456
Size: 3317
Fragments arranged by size:
3317 918 811 249 217 44 42 22
1
EcoRI G'AATT_C
Cuts at: 129 2625 129
Size: 2496 3125
EcoRV GAT'ATC
Cuts at: 984 1969 2530 984
Size: 985 561 4075
Fragments arranged by size:
4075 985 561
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 897 1248 3640 897
Size: 351 2392 2878
Fragments arranged by size:
2878 2392 351
FseI GG_CCGG'CC
Cuts at: 1308 1308
Size: 5621
FspI TGC'GCA
Cuts at: 3402 3402
Size: 5621
HaeII r_GCGC'y
Cuts at: 1348 1814 1831 3055 3303 4325 5304 1348
Size: 466 17 1224 248 1022 979 1665
Fragments arranged by size:
1665 1224 1022 979 466 248 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 434 466 3463 3493 3495 3525 434
Size: 32 2997 30 2 30 2530
Fragments arranged by size:
2997 2530 32 30 30 2
HincII GTy'rAC
Cuts at: 570 2235 2660 570
Size: 1665 425 3531
Fragments arranged by size:
3531 1665 425
HindIII A'AGCT_T
Cuts at: 868 1450 2492 868
Size: 582 1042 3997
Fragments arranged by size:
3997 1042 582
KasI G'GCGC_C
Cuts at: 1344 3051 3299 1344
Size: 1707 248 3666
Fragments arranged by size:
3666 1707 248
KpnI G_GTAC'C
Cuts at: 2641 2641
Size: 5621
MluI A'CGCG_T
Cuts at: 5054 5054
Size: 5621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1949 4292 4476 1949
Size: 2343 184 3094
Fragments arranged by size:
3094 2343 184
MscI TGG'CCA
Cuts at: 1321 3382 1321
Size: 2061 3560
MslI CAynn'nnrTG
Cuts at: 721 3610 3737 721
Size: 2889 127 2605
Fragments arranged by size:
2889 2605 127
MspA1I CmG'CkG
Cuts at: 1317 1325 1528 1808 2732 3046 3406 4419 4664
Size: 8 203 280 924 314 360 1013 245
Cuts at: 4664 1317
Size: 2274
Fragments arranged by size:
2274 1013 924 360 314 280 245 203
8
NaeI GCC'GGC
Cuts at: 1306 3802 1306
Size: 2496 3125
NarI GG'CG_CC
Cuts at: 1345 3052 3300 1345
Size: 1707 248 3666
Fragments arranged by size:
3666 1707 248
NcoI C'CATG_G
Cuts at: 1070 1634 2448 1070
Size: 564 814 4243
Fragments arranged by size:
4243 814 564
NgoMIV G'CCGG_C
Cuts at: 1304 3800 1304
Size: 2496 3125
NheI G'CTAG_C
Cuts at: 2001 2392 2001
Size: 391 5230
NsiI A_TGCA'T
Cuts at: 788 788
Size: 5621
NspI r_CATG'y
Cuts at: 781 1864 2674 3174 4081 781
Size: 1083 810 500 907 2321
Fragments arranged by size:
2321 1083 907 810 500
PasI CC'CwG_GG
Cuts at: 2104 2104
Size: 5621
PciI A'CATG_T
Cuts at: 4077 4077
Size: 5621
PflMI CCAn_nnn'nTGG
Cuts at: 1201 2281 1201
Size: 1080 4541
PfoI T'CCnGG_A
Cuts at: 2125 2125
Size: 5621
PmeI GTTT'AAAC
Cuts at: 2620 2620
Size: 5621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 992 1024 4760 4792 992
Size: 32 3736 32 1821
Fragments arranged by size:
3736 1821 32 32
PpuMI rG'GwC_Cy
Cuts at: 498 1687 498
Size: 1189 4432
PsiI TTA'TAA
Cuts at: 662 662
Size: 5621
PspOMI G'GGCC_C
Cuts at: 455 746 2542 455
Size: 291 1796 3534
Fragments arranged by size:
3534 1796 291
PstI C_TGCA'G
Cuts at: 2463 2668 3353 2463
Size: 205 685 4731
Fragments arranged by size:
4731 685 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5621
PvuII CAG'CTG
Cuts at: 1528 3046 3406 1528
Size: 1518 360 3743
Fragments arranged by size:
3743 1518 360
RsrII CG'GwC_CG
Cuts at: 3816 3816
Size: 5621
SacI G_AGCT'C
Cuts at: 293 2635 293
Size: 2342 3279
SalI G'TCGA_C
Cuts at: 2658 2658
Size: 5621
SapI GCTCTTCn'nnn_
Cuts at: 3644 3854 3644
Size: 210 5411
SbfI CC_TGCA'GG
Cuts at: 2668 2668
Size: 5621
ScaI AGT'ACT
Cuts at: 5138 5138
Size: 5621
SexAI A'CCwGG_T
Cuts at: 1200 1200
Size: 5621
SfcI C'TryA_G
Cuts at: 33 1610 2019 2459 2468 2565 2664 3349 4342
Size: 1577 409 440 9 97 99 685 993
Cuts at: 4342 4533 33
Size: 191 1121
Fragments arranged by size:
1577 1121 993 685 440 409 191 99
97 9
SfoI GGC'GCC
Cuts at: 1346 3053 3301 1346
Size: 1707 248 3666
Fragments arranged by size:
3666 1707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
SmaI CCC'GGG
Cuts at: 2643 2643
Size: 5621
SmlI C'TyrA_G
Cuts at: 466 819 871 890 1278 2776 3691 4183 4445
Size: 353 52 19 388 1498 915 492 262
Cuts at: 4445 466
Size: 1642
Fragments arranged by size:
1642 1498 915 492 388 353 262 52
19
SnaBI TAC'GTA
Cuts at: 2613 2613
Size: 5621
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5621
SphI G_CATG'C
Cuts at: 1864 2674 3174 1864
Size: 810 500 4311
Fragments arranged by size:
4311 810 500
StyI C'CwwG_G
Cuts at: 223 801 1070 1232 1634 2448 2755 3109 223
Size: 578 269 162 402 814 307 354 2735
Fragments arranged by size:
2735 814 578 402 354 307 269 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3204 3337 3796 4005 3204
Size: 133 459 209 4820
Fragments arranged by size:
4820 459 209 133
TatI w'GTAC_w
Cuts at: 1944 2199 5136 1944
Size: 255 2937 2429
Fragments arranged by size:
2937 2429 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1351 1383 5138 5170 1351
Size: 32 3755 32 1802
Fragments arranged by size:
3755 1802 32 32
Tth111I GACn'n_nGTC
Cuts at: 3418 3418
Size: 5621
XbaI T'CTAG_A
Cuts at: 2652 2652
Size: 5621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1632 3116 1632
Size: 1484 4137
XmaI C'CCGG_G
Cuts at: 2641 2641
Size: 5621
XmnI GAAnn'nnTTC
Cuts at: 401 1007 1091 401
Size: 606 84 4931
Fragments arranged by size:
4931 606 84
ZraI GAC'GTC
Cuts at: 1602 1602
Size: 5621
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AscI AvrII BaeI
BbsI BmgBI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII FspAI HpaI MfeI NdeI NotI
NruI PacI PmlI PshAI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI StuI SwaI XhoI