Restriction map for
pF1KSDA0020
(Circular) (Six-base) MAPSORT of: pF1KSDA0020.seq Check: 9485 from: 1 to: 4661
pF1KSDA0020 4661 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 773 1699 773
Size: 926 3735
Acc65I G'GTAC_C
Cuts at: 1677 1677
Size: 4661
AclI AA'CG_TT
Cuts at: 4501 4501
Size: 4661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 1495 2486 2918 3665 3933 1399
Size: 96 991 432 747 268 2127
Fragments arranged by size:
2127 991 747 432 268 96
AflIII A'CryG_T
Cuts at: 2013 3117 4094 2013
Size: 1104 977 2580
Fragments arranged by size:
2580 1104 977
AgeI A'CCGG_T
Cuts at: 3049 3049
Size: 4661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1358 1390 1462 1494 1358
Size: 32 72 32 4525
Fragments arranged by size:
4525 72 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1966 3533 1966
Size: 1567 3094
ApaLI G'TGCA_C
Cuts at: 3431 3431
Size: 4661
ApoI r'AATT_y
Cuts at: 367 1625 1665 4014 4270 367
Size: 1258 40 2349 256 758
Fragments arranged by size:
2349 1258 758 256 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 4661
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
AvaI C'yCGr_G
Cuts at: 1681 1681
Size: 4661
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1118 1151 1118
Size: 33 4628
BamHI G'GATC_C
Cuts at: 1686 1686
Size: 4661
BanI G'GyrC_C
Cuts at: 796 1501 1677 2091 2339 2374 796
Size: 705 176 414 248 35 3083
Fragments arranged by size:
3083 705 414 248 176 35
BanII G_rGCy'C
Cuts at: 530 1151 1675 2705 530
Size: 621 524 1030 2486
Fragments arranged by size:
2486 1030 621 524
BbeI G_GCGC'C
Cuts at: 800 2095 2343 800
Size: 1295 248 3118
Fragments arranged by size:
3118 1295 248
BbsI GAAGACnn'nnnn_
Cuts at: 874 874
Size: 4661
BciVI GTATCCnnnnn_n'
Cuts at: 2559 3320 4661 2559
Size: 761 1341 2559
Fragments arranged by size:
2559 1341 761
BclI T'GATC_A
Cuts at: 2181 2181
Size: 4661
BglI GCCn_nnn'nGGC
Cuts at: 805 1300 805
Size: 495 4166
BglII A'GATC_T
Cuts at: 2176 4213 2176
Size: 2037 2624
BlpI GC'TnA_GC
Cuts at: 1773 1773
Size: 4661
Bme1580I G_kGCm'C
Cuts at: 2286 2379 3435 2286
Size: 93 1056 3512
Fragments arranged by size:
3512 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1873 1954 2289 4433 1873
Size: 81 335 2144 2101
Fragments arranged by size:
2144 2101 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 1283 1510 1151
Size: 132 227 4302
Fragments arranged by size:
4302 227 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 2716 3208 3506 1837
Size: 879 492 298 2992
Fragments arranged by size:
2992 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4365 4365
Size: 4661
BsaAI yAC'GTr
Cuts at: 429 1653 2014 429
Size: 1224 361 3076
Fragments arranged by size:
3076 1224 361
BsaBI GATnn'nnATC
Cuts at: 6 2175 4212 6
Size: 2169 2037 455
Fragments arranged by size:
2169 2037 455
BsaHI Gr'CG_yC
Cuts at: 797 2092 2340 3042 4538 797
Size: 1295 248 702 1496 920
Fragments arranged by size:
1496 1295 920 702 248
BsaWI w'CCGG_w
Cuts at: 1850 2371 3049 3323 3470 3881 1850
Size: 521 678 274 147 411 2630
Fragments arranged by size:
2630 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1133 1163 1413 1443 1133
Size: 30 250 30 4351
Fragments arranged by size:
4351 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1513 1513
Size: 4661
BseYI C'CCAG_C
Cuts at: 2086 2844 3421 3917 2086
Size: 758 577 496 2830
Fragments arranged by size:
2830 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 1550 1043
Size: 507 4154
BsiEI CG_ry'CG
Cuts at: 104 2203 2249 3457 104
Size: 2099 46 1208 1308
Fragments arranged by size:
2099 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 530 1151 1648 1675 2453 2643 3435 530
Size: 621 497 27 778 190 792 1756
Fragments arranged by size:
1756 792 778 621 497 190 27
BsmI GAATG_Cn'
Cuts at: 1290 1290
Size: 4661
BsmBI CGTCTCn'nnnn_
Cuts at: 1259 1259
Size: 4661
Bsp1286I G_dGCh'C
Cuts at: 530 1151 1648 1675 2286 2379 2453 2643 2705
Size: 621 497 27 611 93 74 190 62
Cuts at: 2705 3435 530
Size: 730 1756
Fragments arranged by size:
1756 730 621 611 497 190 93 74
62 27
BspEI T'CCGG_A
Cuts at: 3881 3881
Size: 4661
BspHI T'CATG_A
Cuts at: 3837 3837
Size: 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1193 1711 1925 2227 2608 1193
Size: 518 214 302 381 3246
Fragments arranged by size:
3246 518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2953 3007 4491 4656 50
Size: 2903 54 1484 165 55
Fragments arranged by size:
2903 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 988 1346 2573 988
Size: 358 1227 3076
Fragments arranged by size:
3076 1227 358
BsrFI r'CCGG_y
Cuts at: 2659 2840 3049 2659
Size: 181 209 4271
Fragments arranged by size:
4271 209 181
BssSI C'ACGA_G
Cuts at: 245 2932 3290 245
Size: 2687 358 1616
Fragments arranged by size:
2687 1616 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2038 2038
Size: 4661
BstBI TT'CG_AA
Cuts at: 110 3022 110
Size: 2912 1749
BstYI r'GATC_y
Cuts at: 1 1053 1252 1686 2153 2176 2511 2757 3769
Size: 1052 199 434 467 23 335 246 1012
Cuts at: 3769 3855 3867 4213 1
Size: 86 12 346 449
Fragments arranged by size:
1052 1012 467 449 434 346 335 246
199 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1275 2611 2792 2955 1275
Size: 1336 181 163 2981
Fragments arranged by size:
2981 1336 181 163
BtsI GCAGTG_nn'
Cuts at: 813 1904 813
Size: 1091 3570
DraI TTT'AAA
Cuts at: 420 1660 420
Size: 1240 3421
DrdI GACnn_nn'nnGTC
Cuts at: 2367 3225 2367
Size: 858 3803
EaeI y'GGCC_r
Cuts at: 806 894 2246 2420 2811 2838 4183 806
Size: 88 1352 174 391 27 1345 1284
Fragments arranged by size:
1352 1345 1284 391 174 88 27
EagI C'GGCC_G
Cuts at: 2246 2246
Size: 4661
EarI CTCTTCn'nnn_
Cuts at: 518 2684 2894 518
Size: 2166 210 2285
Fragments arranged by size:
2285 2166 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2869 3179 3325 4471 2869
Size: 310 146 1146 3059
Fragments arranged by size:
3059 1146 310 146
EcoICRI GAG'CTC
Cuts at: 528 1149 1673 528
Size: 621 524 3516
Fragments arranged by size:
3516 621 524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 1283 1399 1495 1510 2486 2918 3665 3933
Size: 132 116 96 15 976 432 747 268
Cuts at: 3933 1151
Size: 1879
Fragments arranged by size:
1879 976 747 432 268 132 116 96
15
EcoO109I rG'GnC_Cy
Cuts at: 1026 1800 1026
Size: 774 3887
EcoRI G'AATT_C
Cuts at: 1665 1665
Size: 4661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4661
FspI TGC'GCA
Cuts at: 2442 2442
Size: 4661
HaeII r_GCGC'y
Cuts at: 800 2095 2343 3365 4344 800
Size: 1295 248 1022 979 1117
Fragments arranged by size:
1295 1117 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 630 662 1133 1165 2503 2533 2535 2565 630
Size: 32 471 32 1338 30 2 30 2726
Fragments arranged by size:
2726 1338 471 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1700 1700
Size: 4661
HindIII A'AGCT_T
Cuts at: 949 1575 949
Size: 626 4035
KasI G'GCGC_C
Cuts at: 796 2091 2339 796
Size: 1295 248 3118
Fragments arranged by size:
3118 1295 248
KpnI G_GTAC'C
Cuts at: 1681 1681
Size: 4661
MluI A'CGCG_T
Cuts at: 4094 4094
Size: 4661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3332 3516 3332
Size: 184 4477
MscI TGG'CCA
Cuts at: 808 896 2422 808
Size: 88 1526 3047
Fragments arranged by size:
3047 1526 88
MslI CAynn'nnrTG
Cuts at: 1322 2650 2777 1322
Size: 1328 127 3206
Fragments arranged by size:
3206 1328 127
MspA1I CmG'CkG
Cuts at: 1772 2086 2446 3459 3704 1772
Size: 314 360 1013 245 2729
Fragments arranged by size:
2729 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2842 2842
Size: 4661
NarI GG'CG_CC
Cuts at: 797 2092 2340 797
Size: 1295 248 3118
Fragments arranged by size:
3118 1295 248
NgoMIV G'CCGG_C
Cuts at: 2840 2840
Size: 4661
NspI r_CATG'y
Cuts at: 451 516 1459 1714 2214 3121 451
Size: 65 943 255 500 907 1991
Fragments arranged by size:
1991 943 907 500 255 65
PciI A'CATG_T
Cuts at: 3117 3117
Size: 4661
PflMI CCAn_nnn'nTGG
Cuts at: 1206 1206
Size: 4661
PmeI GTTT'AAAC
Cuts at: 1660 1660
Size: 4661
PmlI CAC'GTG
Cuts at: 429 429
Size: 4661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4629
PpuMI rG'GwC_Cy
Cuts at: 1026 1026
Size: 4661
PstI C_TGCA'G
Cuts at: 1365 1708 2393 1365
Size: 343 685 3633
Fragments arranged by size:
3633 685 343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4661
PvuII CAG'CTG
Cuts at: 2086 2446 2086
Size: 360 4301
RsrII CG'GwC_CG
Cuts at: 2856 2856
Size: 4661
SacI G_AGCT'C
Cuts at: 530 1151 1675 530
Size: 621 524 3516
Fragments arranged by size:
3516 621 524
SalI G'TCGA_C
Cuts at: 1698 1698
Size: 4661
SapI GCTCTTCn'nnn_
Cuts at: 518 2684 2894 518
Size: 2166 210 2285
Fragments arranged by size:
2285 2166 210
SbfI CC_TGCA'GG
Cuts at: 1365 1708 1365
Size: 343 4318
ScaI AGT'ACT
Cuts at: 4178 4178
Size: 4661
SfcI C'TryA_G
Cuts at: 33 1361 1704 2389 3382 3573 33
Size: 1328 343 685 993 191 1121
Fragments arranged by size:
1328 1121 993 685 343 191
SfoI GGC'GCC
Cuts at: 798 2093 2341 798
Size: 1295 248 3118
Fragments arranged by size:
3118 1295 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
SmaI CCC'GGG
Cuts at: 1683 1683
Size: 4661
SmlI C'TyrA_G
Cuts at: 1816 2731 3223 3485 1816
Size: 915 492 262 2992
Fragments arranged by size:
2992 915 492 262
SnaBI TAC'GTA
Cuts at: 1653 1653
Size: 4661
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4661
SphI G_CATG'C
Cuts at: 451 1714 2214 451
Size: 1263 500 2898
Fragments arranged by size:
2898 1263 500
SspI AAT'ATT
Cuts at: 346 357 346
Size: 11 4650
StyI C'CwwG_G
Cuts at: 836 1795 2149 836
Size: 959 354 3348
Fragments arranged by size:
3348 959 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2244 2377 2836 3045 2244
Size: 133 459 209 3860
Fragments arranged by size:
3860 459 209 133
TatI w'GTAC_w
Cuts at: 1034 4176 1034
Size: 3142 1519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 4629
Tth111I GACn'n_nGTC
Cuts at: 2458 2458
Size: 4661
XbaI T'CTAG_A
Cuts at: 1692 1692
Size: 4661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1296 2156 1296
Size: 860 3801
XmaI C'CCGG_G
Cuts at: 1681 1681
Size: 4661
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
XmnI ZraI