Restriction map for
pF1KSDA0022
(Circular) (Six-base) MAPSORT of: pF1KSDA0022.seq Check: 7146 from: 1 to: 3835
pF1KSDA0022 3835 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3835
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3835
AclI AA'CG_TT
Cuts at: 3675 3675
Size: 3835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2839 3107 1658
Size: 432 749 268 2386
Fragments arranged by size:
2386 749 432 268
AflIII A'CryG_T
Cuts at: 1185 2291 3268 1185
Size: 1106 977 1752
Fragments arranged by size:
1752 1106 977
AgeI A'CCGG_T
Cuts at: 2223 2223
Size: 3835
AlwNI CAG_nnn'CTG
Cuts at: 1138 2707 1138
Size: 1569 2266
ApaLI G'TGCA_C
Cuts at: 2605 2605
Size: 3835
ApoI r'AATT_y
Cuts at: 837 3188 3444 837
Size: 2351 256 1228
Fragments arranged by size:
2351 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3835
AvaI C'yCGr_G
Cuts at: 853 853
Size: 3835
BamHI G'GATC_C
Cuts at: 858 858
Size: 3835
BanI G'GyrC_C
Cuts at: 849 1263 1511 1546 849
Size: 414 248 35 3138
Fragments arranged by size:
3138 414 248 35
BanII G_rGCy'C
Cuts at: 847 1877 847
Size: 1030 2805
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3587
BbsI GAAGACnn'nnnn_
Cuts at: 776 776
Size: 3835
BciVI GTATCCnnnnn_n'
Cuts at: 348 1731 2494 3835 348
Size: 1383 763 1341 348
Fragments arranged by size:
1383 1341 763 348
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3835
BglII A'GATC_T
Cuts at: 1348 3387 1348
Size: 2039 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3835
Bme1580I G_kGCm'C
Cuts at: 1458 1551 2609 1458
Size: 93 1058 2684
Fragments arranged by size:
2684 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3607 1045
Size: 81 335 2146 1273
Fragments arranged by size:
2146 1273 335 81
BmtI G_CTAG'C
Cuts at: 659 659
Size: 3835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2382 2680 1009
Size: 879 494 298 2164
Fragments arranged by size:
2164 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3539 3539
Size: 3835
BsaAI yAC'GTr
Cuts at: 825 1186 825
Size: 361 3474
BsaBI GATnn'nnATC
Cuts at: 6 1347 3386 6
Size: 1341 2039 455
Fragments arranged by size:
2039 1341 455
BsaHI Gr'CG_yC
Cuts at: 1264 1512 2216 3712 1264
Size: 248 704 1496 1387
Fragments arranged by size:
1496 1387 704 248
BsaWI w'CCGG_w
Cuts at: 1022 1543 2223 2497 2644 3055 1022
Size: 521 680 274 147 411 1802
Fragments arranged by size:
1802 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 165 1258 2016 2595 3091 165
Size: 1093 758 579 496 909
Fragments arranged by size:
1093 909 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 502
Size: 3835
BsiEI CG_ry'CG
Cuts at: 104 1375 1421 2631 104
Size: 1271 46 1210 1308
Fragments arranged by size:
1308 1271 1210 46
BsiHKAI G_wGCw'C
Cuts at: 847 1625 1815 2609 847
Size: 778 190 794 2073
Fragments arranged by size:
2073 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 847 1458 1551 1625 1815 1877 2609 847
Size: 611 93 74 190 62 732 2073
Fragments arranged by size:
2073 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3055 3055
Size: 3835
BspHI T'CATG_A
Cuts at: 3011 3011
Size: 3835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 883 1097 1399 1780 883
Size: 214 302 381 2938
Fragments arranged by size:
2938 381 302 214
BsrBI CCG'CTC
Cuts at: 50 307 2125 2179 3665 3830 50
Size: 257 1818 54 1486 165 55
Fragments arranged by size:
1818 1486 257 165 55 54
BsrDI GCAATG_nn'
Cuts at: 376 1745 376
Size: 1369 2466
BsrFI r'CCGG_y
Cuts at: 1831 2012 2223 1831
Size: 181 211 3443
Fragments arranged by size:
3443 211 181
BsrGI T'GTAC_A
Cuts at: 701 701
Size: 3835
BssSI C'ACGA_G
Cuts at: 2104 2464 2104
Size: 360 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3835
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3835
BstYI r'GATC_y
Cuts at: 1 858 1325 1348 1683 1929 2943 3029 3041
Size: 857 467 23 335 246 1014 86 12
Cuts at: 3041 3387 1
Size: 346 449
Fragments arranged by size:
1014 857 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 300 300
Size: 3835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3491
Fragments arranged by size:
3491 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3835
DraI TTT'AAA
Cuts at: 832 832
Size: 3835
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2399 1539
Size: 860 2975
EaeI y'GGCC_r
Cuts at: 1418 1592 1983 2010 3357 1418
Size: 174 391 27 1347 1896
Fragments arranged by size:
1896 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3835
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2353 2499 3645 2041
Size: 312 146 1146 2231
Fragments arranged by size:
2231 1146 312 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2839 3107 1658
Size: 432 749 268 2386
Fragments arranged by size:
2386 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 972 972
Size: 3835
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3835
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3835
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3835
HaeII r_GCGC'y
Cuts at: 1267 1515 2539 3518 1267
Size: 248 1024 979 1584
Fragments arranged by size:
1584 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1675 1705 1707 1737 1675
Size: 30 2 30 3773
Fragments arranged by size:
3773 30 30 2
HincII GTy'rAC
Cuts at: 501 872 501
Size: 371 3464
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3587
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3835
MluI A'CGCG_T
Cuts at: 3268 3268
Size: 3835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 768 2506 2690 768
Size: 1738 184 1913
Fragments arranged by size:
1913 1738 184
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3835
MslI CAynn'nnrTG
Cuts at: 1822 1949 1822
Size: 127 3708
MspA1I CmG'CkG
Cuts at: 178 944 1258 1618 2633 2878 178
Size: 766 314 360 1015 245 1135
Fragments arranged by size:
1135 1015 766 360 314 245
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3835
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3587
NcoI C'CATG_G
Cuts at: 300 300
Size: 3835
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3835
NheI G'CTAG_C
Cuts at: 655 655
Size: 3835
NruI TCG'CGA
Cuts at: 2196 2196
Size: 3835
NspI r_CATG'y
Cuts at: 405 886 1386 2295 405
Size: 481 500 909 1945
Fragments arranged by size:
1945 909 500 481
PciI A'CATG_T
Cuts at: 2291 2291
Size: 3835
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 3006 2974
Size: 32 3803
PsiI TTA'TAA
Cuts at: 764 764
Size: 3835
PstI C_TGCA'G
Cuts at: 880 1565 880
Size: 685 3150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3835
PvuII CAG'CTG
Cuts at: 1258 1618 1258
Size: 360 3475
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3835
SacI G_AGCT'C
Cuts at: 847 847
Size: 3835
SalI G'TCGA_C
Cuts at: 870 870
Size: 3835
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3625
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3835
ScaI AGT'ACT
Cuts at: 3352 3352
Size: 3835
SfcI C'TryA_G
Cuts at: 33 876 1561 2556 2747 33
Size: 843 685 995 191 1121
Fragments arranged by size:
1121 995 843 685 191
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3587
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3835
SmaI CCC'GGG
Cuts at: 855 855
Size: 3835
SmlI C'TyrA_G
Cuts at: 988 1903 2397 2659 988
Size: 915 494 262 2164
Fragments arranged by size:
2164 915 494 262
SnaBI TAC'GTA
Cuts at: 825 825
Size: 3835
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3835
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3335
SspI AAT'ATT
Cuts at: 613 613
Size: 3835
StyI C'CwwG_G
Cuts at: 300 967 1321 300
Size: 667 354 2814
Fragments arranged by size:
2814 667 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1549 2008 2219 1416
Size: 133 459 211 3032
Fragments arranged by size:
3032 459 211 133
TatI w'GTAC_w
Cuts at: 292 701 3350 292
Size: 409 2649 777
Fragments arranged by size:
2649 777 409
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3352 3384 3352
Size: 32 3803
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3835
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3835
XmaI C'CCGG_G
Cuts at: 853 853
Size: 3835
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI ZraI