Restriction map for
pF1KSDA0023
(Circular) (Six-base) MAPSORT of: pF1KSDA0023.seq Check: 1125 from: 1 to: 9416
pF1KSDA0023 9416 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1521 2645 4821 1521
Size: 1124 2176 6116
Fragments arranged by size:
6116 2176 1124
AatII G_ACGT'C
Cuts at: 3097 4159 3097
Size: 1062 8354
AccI GT'mk_AC
Cuts at: 1226 6454 1226
Size: 5228 4188
Acc65I G'GTAC_C
Cuts at: 874 4969 5203 5524 6432 874
Size: 4095 234 321 908 3858
Fragments arranged by size:
4095 3858 908 321 234
AclI AA'CG_TT
Cuts at: 3436 9256 3436
Size: 5820 3596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2128 2509 2722 3230 3617 5280 5564 5703 6187
Size: 381 213 508 387 1663 284 139 484
Cuts at: 6187 7241 7673 8420 8688 2128
Size: 1054 432 747 268 2856
Fragments arranged by size:
2856 1663 1054 747 508 484 432 387
381 284 268 213 139
AfeI AGC'GCT
Cuts at: 188 188
Size: 9416
AflII C'TTAA_G
Cuts at: 3577 3577
Size: 9416
AflIII A'CryG_T
Cuts at: 6768 7872 8849 6768
Size: 1104 977 7335
Fragments arranged by size:
7335 1104 977
AgeI A'CCGG_T
Cuts at: 7804 7804
Size: 9416
AleI CACnn'nnGTG
Cuts at: 1807 5077 1807
Size: 3270 6146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1825 3383 6196 1825
Size: 1558 2813 5045
Fragments arranged by size:
5045 2813 1558
AlwNI CAG_nnn'CTG
Cuts at: 1084 1464 1489 1669 2194 2610 3072 3538 3724
Size: 380 25 180 525 416 462 466 186
Cuts at: 3724 4858 4879 5059 5155 6721 8288 1084
Size: 1134 21 180 96 1566 1567 2212
Fragments arranged by size:
2212 1567 1566 1134 525 466 462 416
380 186 180 180 96 25 21
ApaLI G'TGCA_C
Cuts at: 3326 8186 3326
Size: 4860 4556
ApoI r'AATT_y
Cuts at: 400 2531 2557 3570 6420 8769 9025 400
Size: 2131 26 1013 2850 2349 256 791
Fragments arranged by size:
2850 2349 2131 1013 791 256 26
AseI AT'TA_AT
Cuts at: 20 1133 1337 20
Size: 1113 204 8099
Fragments arranged by size:
8099 1113 204
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9416
AvaI C'yCGr_G
Cuts at: 154 240 6436 154
Size: 86 6196 3134
Fragments arranged by size:
6196 3134 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 866 899 866
Size: 33 9383
BamHI G'GATC_C
Cuts at: 2502 4321 5794 6013 6441 2502
Size: 1819 1473 219 428 5477
Fragments arranged by size:
5477 1819 1473 428 219
BanI G'GyrC_C
Cuts at: 874 2537 2633 3405 3951 4969 5203 5380 5524
Size: 1663 96 772 546 1018 234 177 144
Cuts at: 5524 6033 6432 6846 7094 7129 874
Size: 509 399 414 248 35 3161
Fragments arranged by size:
3161 1663 1018 772 546 509 414 399
248 234 177 144 96 35
BanII G_rGCy'C
Cuts at: 730 1004 1560 1622 4220 6079 6430 7460 730
Size: 274 556 62 2598 1859 351 1030 2686
Fragments arranged by size:
2686 2598 1859 1030 556 351 274 62
BbeI G_GCGC'C
Cuts at: 2541 6850 7098 2541
Size: 4309 248 4859
Fragments arranged by size:
4859 4309 248
BbsI GAAGACnn'nnnn_
Cuts at: 270 876 2728 2962 3035 3752 5420 270
Size: 606 1852 234 73 717 1668 4266
Fragments arranged by size:
4266 1852 1668 717 606 234 73
BbvCI CC'TCA_GC
Cuts at: 5529 5529
Size: 9416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 124 158 6078 6112 124
Size: 34 5920 34 3428
Fragments arranged by size:
5920 3428 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1117 7314 8075 9416 1117
Size: 6197 761 1341 1117
Fragments arranged by size:
6197 1341 1117 761
BclI T'GATC_A
Cuts at: 1125 6936 1125
Size: 5811 3605
BglI GCCn_nnn'nGGC
Cuts at: 3361 3403 3588 6074 3361
Size: 42 185 2486 6703
Fragments arranged by size:
6703 2486 185 42
BglII A'GATC_T
Cuts at: 6931 8968 6931
Size: 2037 7379
BlpI GC'TnA_GC
Cuts at: 2519 5000 5025 6528 2519
Size: 2481 25 1503 5407
Fragments arranged by size:
5407 2481 1503 25
Bme1580I G_kGCm'C
Cuts at: 2072 2290 3330 4169 4415 6109 7041 7134 8190
Size: 218 1040 839 246 1694 932 93 1056
Cuts at: 8190 2072
Size: 3298
Fragments arranged by size:
3298 1694 1056 1040 932 839 246 218
93
BmgBI CAC'GTC
Cuts at: 4925 4925
Size: 9416
BmrI ACTGGGnnnn_n'
Cuts at: 1622 2059 3060 3164 3514 3526 5735 6628 6709
Size: 437 1001 104 350 12 2209 893 81
Cuts at: 6709 7044 9188 1622
Size: 335 2144 1850
Fragments arranged by size:
2209 2144 1850 1001 893 437 350 335
104 81 12
BmtI G_CTAG'C
Cuts at: 3266 3266
Size: 9416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 6173 7783 2534
Size: 3639 1610 4167
Fragments arranged by size:
4167 3639 1610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 914 1174 1268 2090 2966 3094 4064 4899
Size: 170 260 94 822 876 128 970 835
Cuts at: 4899 5388 5930 5966 744
Size: 489 542 36 4194
Fragments arranged by size:
4194 970 876 835 822 542 489 260
170 128 94 36
Bpu10I CC'TnA_GC
Cuts at: 2048 5178 5529 2048
Size: 3130 351 5935
Fragments arranged by size:
5935 3130 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 1433 2431 3263 3437 4851 5373 6592 7471
Size: 989 998 832 174 1414 522 1219 879
Cuts at: 7471 7963 8261 444
Size: 492 298 1599
Fragments arranged by size:
1599 1414 1219 998 989 879 832 522
492 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 2353 3566 4596 9120 2353
Size: 1213 1030 4524 2649
Fragments arranged by size:
4524 2649 1213 1030
BsaAI yAC'GTr
Cuts at: 6408 6769 6408
Size: 361 9055
BsaBI GATnn'nnATC
Cuts at: 6 2189 2606 6930 8967 6
Size: 2183 417 4324 2037 455
Fragments arranged by size:
4324 2183 2037 455 417
BsaHI Gr'CG_yC
Cuts at: 2538 3094 4156 6847 7095 7797 9293 2538
Size: 556 1062 2691 248 702 1496 2661
Fragments arranged by size:
2691 2661 1496 1062 702 556 248
BsaWI w'CCGG_w
Cuts at: 3604 6605 7126 7804 8078 8225 8636 3604
Size: 3001 521 678 274 147 411 4384
Fragments arranged by size:
4384 3001 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 563 593 1208 1238 1260 1290 4612 4642 5498
Size: 30 615 30 22 30 3322 30 856
Cuts at: 5498 5528 5906 5936 6077 6107 563
Size: 30 378 30 141 30 3872
Fragments arranged by size:
3872 3322 856 615 378 141 30 30
30 30 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1434 2230 2311 4633 4640 5927 6218 1434
Size: 796 81 2322 7 1287 291 4632
Fragments arranged by size:
4632 2322 1287 796 291 81 7
BseYI C'CCAG_C
Cuts at: 4011 4142 4634 4694 4767 5193 6054 6841 7599
Size: 131 492 60 73 426 861 787 758
Cuts at: 7599 8176 8672 4011
Size: 577 496 4755
Fragments arranged by size:
4755 861 787 758 577 496 492 426
131 73 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 3487 3761 4337 4892 1002
Size: 2485 274 576 555 5526
Fragments arranged by size:
5526 2485 576 555 274
BsiEI CG_ry'CG
Cuts at: 104 6958 7004 8212 104
Size: 6854 46 1208 1308
Fragments arranged by size:
6854 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3330 6430 7208 7398 8190 3330
Size: 3100 778 190 792 4556
Fragments arranged by size:
4556 3100 792 778 190
BsmI GAATG_Cn'
Cuts at: 2807 3823 6040 6355 2807
Size: 1016 2217 315 5868
Fragments arranged by size:
5868 2217 1016 315
BsmBI CGTCTCn'nnnn_
Cuts at: 125 932 1739 125
Size: 807 807 7802
Fragments arranged by size:
7802 807 807
Bsp1286I G_dGCh'C
Cuts at: 730 1004 1560 1622 2072 2290 3330 4169 4220
Size: 274 556 62 450 218 1040 839 51
Cuts at: 4220 4415 6079 6109 6430 7041 7134 7208 7398
Size: 195 1664 30 321 611 93 74 190
Cuts at: 7398 7460 8190 730
Size: 62 730 1956
Fragments arranged by size:
1956 1664 1040 839 730 611 556 450
321 274 218 195 190 93 74 62
62 51 30
BspEI T'CCGG_A
Cuts at: 8636 8636
Size: 9416
BspHI T'CATG_A
Cuts at: 8592 8592
Size: 9416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1521 2645 3933 4821 6466 6680 6982 7363 1521
Size: 1124 1288 888 1645 214 302 381 3574
Fragments arranged by size:
3574 1645 1288 1124 888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 159 6223 7708 7762 9246 9411 50
Size: 109 6064 1485 54 1484 165 55
Fragments arranged by size:
6064 1485 1484 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1844 2850 7328 1523
Size: 321 1006 4478 3611
Fragments arranged by size:
4478 3611 1006 321
BsrFI r'CCGG_y
Cuts at: 7414 7595 7804 7414
Size: 181 209 9026
Fragments arranged by size:
9026 209 181
BssSI C'ACGA_G
Cuts at: 7687 8045 7687
Size: 358 9058
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1489 3196 4753 6793 1084
Size: 405 1707 1557 2040 3707
Fragments arranged by size:
3707 2040 1707 1557 405
BstBI TT'CG_AA
Cuts at: 110 7777 110
Size: 7667 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 5938 5938
Size: 9416
BstYI r'GATC_y
Cuts at: 1 2190 2502 2971 4321 5794 6013 6250 6441
Size: 2189 312 469 1350 1473 219 237 191
Cuts at: 6441 6908 6931 7266 7512 8524 8610 8622 8968
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8968 1
Size: 449
Fragments arranged by size:
2189 1473 1350 1012 469 467 449 346
335 312 246 237 219 191 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 218 3885 4170 218
Size: 3667 285 5464
Fragments arranged by size:
5464 3667 285
BtgI C'CryG_G
Cuts at: 125 614 698 1211 3143 4775 125
Size: 489 84 513 1932 1632 4766
Fragments arranged by size:
4766 1932 1632 513 489 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7366 7547 7710 7366
Size: 181 163 9072
Fragments arranged by size:
9072 181 163
BtsI GCAGTG_nn'
Cuts at: 755 2975 3122 3770 4718 5057 5280 6107 6659
Size: 2220 147 648 948 339 223 827 552
Cuts at: 6659 755
Size: 3512
Fragments arranged by size:
3512 2220 948 827 648 552 339 223
147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1223 1258 4911 4946 5946 5981 1223
Size: 35 3653 35 1000 35 4658
Fragments arranged by size:
4658 3653 1000 35 35 35
DraI TTT'AAA
Cuts at: 6415 6415
Size: 9416
DraIII CAC_nnn'GTG
Cuts at: 2727 3232 3522 2727
Size: 505 290 8621
Fragments arranged by size:
8621 505 290
DrdI GACnn_nn'nnGTC
Cuts at: 2367 4588 7122 7980 2367
Size: 2221 2534 858 3803
Fragments arranged by size:
3803 2534 2221 858
EaeI y'GGCC_r
Cuts at: 2945 3395 4080 4883 5457 5649 5777 6143 6258
Size: 450 685 803 574 192 128 366 115
Cuts at: 6258 7001 7175 7566 7593 8938 2945
Size: 743 174 391 27 1345 3423
Fragments arranged by size:
3423 1345 803 743 685 574 450 391
366 192 174 128 115 27
EagI C'GGCC_G
Cuts at: 7001 7001
Size: 9416
EarI CTCTTCn'nnn_
Cuts at: 695 1277 1935 2240 2704 4538 5670 5697 7439
Size: 582 658 305 464 1834 1132 27 1742
Cuts at: 7439 7649 695
Size: 210 2462
Fragments arranged by size:
2462 1834 1742 1132 658 582 464 305
210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 7624 7934 8080 9226 2737
Size: 4887 310 146 1146 2927
Fragments arranged by size:
4887 2927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 6428 6428
Size: 9416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 914 1174 1268 2090 2128 2509 2722 2966
Size: 170 260 94 822 38 381 213 244
Cuts at: 2966 3094 3230 3617 4064 4899 5280 5388 5564
Size: 128 136 387 447 835 381 108 176
Cuts at: 5564 5703 5930 5966 6187 7241 7673 8420 8688
Size: 139 227 36 221 1054 432 747 268
Cuts at: 8688 744
Size: 1472
Fragments arranged by size:
1472 1054 835 822 747 447 432 387
381 381 268 260 244 227 221 213
176 170 139 136 128 108 94 38
36
EcoNI CCTnn'n_nnAGG
Cuts at: 3884 3884
Size: 9416
EcoO109I rG'GnC_Cy
Cuts at: 914 1666 3126 4782 4876 6137 6396 6555 914
Size: 752 1460 1656 94 1261 259 159 3775
Fragments arranged by size:
3775 1656 1460 1261 752 259 159 94
EcoRI G'AATT_C
Cuts at: 6420 6420
Size: 9416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1866 1900 2539 3866 7435 1866
Size: 34 639 1327 3569 3847
Fragments arranged by size:
3847 3569 1327 639 34
FspI TGC'GCA
Cuts at: 7197 7197
Size: 9416
HaeII r_GCGC'y
Cuts at: 190 2541 6850 7098 8120 9099 190
Size: 2351 4309 248 1022 979 507
Fragments arranged by size:
4309 2351 1022 979 507 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1017 1049 1260 1292 2088 2120 2703 2735 3384
Size: 32 211 32 796 32 583 32 649
Cuts at: 3384 3416 3811 3843 4612 4644 5498 5530 6235
Size: 32 395 32 769 32 854 32 705
Cuts at: 6235 6267 7258 7288 7290 7320 1017
Size: 32 991 30 2 30 3113
Fragments arranged by size:
3113 991 854 796 769 705 649 583
395 211 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1881 3527 6455 1881
Size: 1646 2928 4842
Fragments arranged by size:
4842 2928 1646
HindIII A'AGCT_T
Cuts at: 553 3629 5576 553
Size: 3076 1947 4393
Fragments arranged by size:
4393 3076 1947
KasI G'GCGC_C
Cuts at: 2537 6846 7094 2537
Size: 4309 248 4859
Fragments arranged by size:
4859 4309 248
KpnI G_GTAC'C
Cuts at: 878 4973 5207 5528 6436 878
Size: 4095 234 321 908 3858
Fragments arranged by size:
4095 3858 908 321 234
MluI A'CGCG_T
Cuts at: 8849 8849
Size: 9416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 1667 2222 4480 6184 8087 8271 284
Size: 1383 555 2258 1704 1903 184 1429
Fragments arranged by size:
2258 1903 1704 1429 1383 555 184
MscI TGG'CCA
Cuts at: 2947 3397 4082 5459 5651 6145 6260 7177 2947
Size: 450 685 1377 192 494 115 917 5186
Fragments arranged by size:
5186 1377 917 685 494 450 192 115
MslI CAynn'nnrTG
Cuts at: 1807 2549 5077 7405 7532 1807
Size: 742 2528 2328 127 3691
Fragments arranged by size:
3691 2528 2328 742 127
MspA1I CmG'CkG
Cuts at: 744 1016 1480 1753 1948 2110 3367 3412 3658
Size: 272 464 273 195 162 1257 45 246
Cuts at: 3658 4441 4528 4858 5155 5533 6115 6527 6841
Size: 783 87 330 297 378 582 412 314
Cuts at: 6841 7201 8214 8459 744
Size: 360 1013 245 1701
Fragments arranged by size:
1701 1257 1013 783 582 464 412 378
360 330 314 297 273 272 246 245
195 162 87 45
NaeI GCC'GGC
Cuts at: 7597 7597
Size: 9416
NarI GG'CG_CC
Cuts at: 2538 6847 7095 2538
Size: 4309 248 4859
Fragments arranged by size:
4859 4309 248
NcoI C'CATG_G
Cuts at: 125 614 1211 125
Size: 489 597 8330
Fragments arranged by size:
8330 597 489
NgoMIV G'CCGG_C
Cuts at: 7595 7595
Size: 9416
NheI G'CTAG_C
Cuts at: 3262 3262
Size: 9416
NruI TCG'CGA
Cuts at: 6341 6341
Size: 9416
NspI r_CATG'y
Cuts at: 6469 6969 7876 6469
Size: 500 907 8009
Fragments arranged by size:
8009 907 500
PasI CC'CwG_GG
Cuts at: 5215 6019 5215
Size: 804 8612
PciI A'CATG_T
Cuts at: 7872 7872
Size: 9416
PflMI CCAn_nnn'nTGG
Cuts at: 417 1669 3072 5200 5989 417
Size: 1252 1403 2128 789 3844
Fragments arranged by size:
3844 2128 1403 1252 789
PmeI GTTT'AAAC
Cuts at: 6415 6415
Size: 9416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8555 8587 8555
Size: 32 9384
PpuMI rG'GwC_Cy
Cuts at: 914 4782 6137 6396 914
Size: 3868 1355 259 3934
Fragments arranged by size:
3934 3868 1355 259
PshAI GACnn'nnGTC
Cuts at: 4846 4846
Size: 9416
PsiI TTA'TAA
Cuts at: 2489 2489
Size: 9416
PspXI vC'TCGA_Gb
Cuts at: 240 240
Size: 9416
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5391 5423 5589 5621 5391
Size: 32 166 32 9186
Fragments arranged by size:
9186 166 32 32
PstI C_TGCA'G
Cuts at: 909 1479 1485 1731 4704 4725 4758 4953 6114
Size: 570 6 246 2973 21 33 195 1161
Cuts at: 6114 6463 7148 909
Size: 349 685 3177
Fragments arranged by size:
3177 2973 1161 685 570 349 246 195
33 21 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9416
PvuII CAG'CTG
Cuts at: 744 1016 1480 1753 1948 3367 3412 3658 4441
Size: 272 464 273 195 1419 45 246 783
Cuts at: 4441 4858 5155 5533 6115 6841 7201 744
Size: 417 297 378 582 726 360 2959
Fragments arranged by size:
2959 1419 783 726 582 464 417 378
360 297 273 272 246 195 45
RsrII CG'GwC_CG
Cuts at: 7611 7611
Size: 9416
SacI G_AGCT'C
Cuts at: 6430 6430
Size: 9416
SalI G'TCGA_C
Cuts at: 6453 6453
Size: 9416
SanDI GG'GwC_CC
Cuts at: 914 4782 6137 6396 914
Size: 3868 1355 259 3934
Fragments arranged by size:
3934 3868 1355 259
SapI GCTCTTCn'nnn_
Cuts at: 7439 7649 7439
Size: 210 9206
SbfI CC_TGCA'GG
Cuts at: 6463 6463
Size: 9416
ScaI AGT'ACT
Cuts at: 1425 4734 5631 8933 1425
Size: 3309 897 3302 1908
Fragments arranged by size:
3309 3302 1908 897
SexAI A'CCwGG_T
Cuts at: 2723 3209 3228 2723
Size: 486 19 8911
Fragments arranged by size:
8911 486 19
SfcI C'TryA_G
Cuts at: 33 905 1475 1481 1727 3500 4436 4700 4721
Size: 872 570 6 246 1773 936 264 21
Cuts at: 4721 4754 4949 6110 6459 7144 8137 8328 33
Size: 33 195 1161 349 685 993 191 1121
Fragments arranged by size:
1773 1161 1121 993 936 872 685 570
349 264 246 195 191 33 21 6
SfoI GGC'GCC
Cuts at: 2539 6848 7096 2539
Size: 4309 248 4859
Fragments arranged by size:
4859 4309 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9416
SmaI CCC'GGG
Cuts at: 6438 6438
Size: 9416
SmlI C'TyrA_G
Cuts at: 240 423 1448 2410 3278 3452 3577 4866 5388
Size: 183 1025 962 868 174 125 1289 522
Cuts at: 5388 6571 7486 7978 8240 240
Size: 1183 915 492 262 1416
Fragments arranged by size:
1416 1289 1183 1025 962 915 868 522
492 262 183 174 125
SnaBI TAC'GTA
Cuts at: 6408 6408
Size: 9416
SpeI A'CTAG_T
Cuts at: 67 4135 4372 4735 67
Size: 4068 237 363 4748
Fragments arranged by size:
4748 4068 363 237
SphI G_CATG'C
Cuts at: 6469 6969 6469
Size: 500 8916
StuI AGG'CCT
Cuts at: 5248 5248
Size: 9416
StyI C'CwwG_G
Cuts at: 125 230 378 614 1211 5727 5931 6194 6255
Size: 105 148 236 597 4516 204 263 61
Cuts at: 6255 6550 6904 125
Size: 295 354 2637
Fragments arranged by size:
4516 2637 597 354 295 263 236 204
148 105 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 989 2106 3533 5320 6999 7132 7591 7800 989
Size: 1117 1427 1787 1679 133 459 209 2605
Fragments arranged by size:
2605 1787 1679 1427 1117 459 209 133
TatI w'GTAC_w
Cuts at: 1423 4732 5629 8931 1423
Size: 3309 897 3302 1908
Fragments arranged by size:
3309 3302 1908 897
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 563 595 850 882 2824 2856 2866 2898 3233
Size: 32 255 32 1942 32 10 32 335
Cuts at: 3233 3265 4437 4469 5906 5938 6077 6109 8933
Size: 32 1172 32 1437 32 139 32 2824
Cuts at: 8933 8965 563
Size: 32 1014
Fragments arranged by size:
2824 1942 1437 1172 1014 335 255 139
32 32 32 32 32 32 32 32
32 10
Tth111I GACn'n_nGTC
Cuts at: 7213 7213
Size: 9416
XbaI T'CTAG_A
Cuts at: 2586 4614 6447 2586
Size: 2028 1833 5555
Fragments arranged by size:
5555 2028 1833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6299 6383 6911 6299
Size: 84 528 8804
Fragments arranged by size:
8804 528 84
XhoI C'TCGA_G
Cuts at: 240 240
Size: 9416
XmaI C'CCGG_G
Cuts at: 6436 6436
Size: 9416
XmnI GAAnn'nnTTC
Cuts at: 4062 4685 4062
Size: 623 8793
ZraI GAC'GTC
Cuts at: 3095 4157 3095
Size: 1062 8354
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AloI ApaI AscI AvrII BfrBI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NsiI PacI PfoI PmlI PspOMI
SacII SfiI SgrAI SrfI SspI SwaI