Restriction map for
pF1KSDA0024
(Circular) (Six-base) MAPSORT of: pF1KSDA0024.seq Check: 8252 from: 1 to: 4556
pF1KSDA0024 4556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 2381 2813 3560 3828 1463
Size: 918 432 747 268 2191
Fragments arranged by size:
2191 918 747 432 268
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1422 1454 1422
Size: 32 4524
AlwNI CAG_nnn'CTG
Cuts at: 1861 3428 1861
Size: 1567 2989
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 489 1094 1234 1560 3909 4165 489
Size: 605 140 326 2349 256 880
Fragments arranged by size:
2349 880 605 326 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 1576 1576
Size: 4556
BamHI G'GATC_C
Cuts at: 1581 1581
Size: 4556
BanI G'GyrC_C
Cuts at: 1390 1572 1986 2234 2269 1390
Size: 182 414 248 35 3677
Fragments arranged by size:
3677 414 248 182 35
BanII G_rGCy'C
Cuts at: 728 1570 2600 728
Size: 842 1030 2684
Fragments arranged by size:
2684 1030 842
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbsI GAAGACnn'nnnn_
Cuts at: 329 1451 329
Size: 1122 3434
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 194 590 1007 2076 194
Size: 396 417 1069 2674
Fragments arranged by size:
2674 1069 417 396
BfrBI ATG'CAT
Cuts at: 183 738 183
Size: 555 4001
BglI GCCn_nnn'nGGC
Cuts at: 661 661
Size: 4556
BglII A'GATC_T
Cuts at: 1245 1406 2071 4108 1245
Size: 161 665 2037 1693
Fragments arranged by size:
2037 1693 665 161
BlpI GC'TnA_GC
Cuts at: 262 1668 262
Size: 1406 3150
Bme1580I G_kGCm'C
Cuts at: 2181 2274 3330 2181
Size: 93 1056 3407
Fragments arranged by size:
3407 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 873 1768 1849 2184 4328 873
Size: 895 81 335 2144 1101
Fragments arranged by size:
2144 1101 895 335 81
BmtI G_CTAG'C
Cuts at: 938 938
Size: 4556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 361 2923 361
Size: 2562 1994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 1010 1234 354
Size: 656 224 3676
Fragments arranged by size:
3676 656 224
Bpu10I CC'TnA_GC
Cuts at: 154 154
Size: 4556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 691 4260 691
Size: 3569 987
BsaAI yAC'GTr
Cuts at: 1548 1909 1548
Size: 361 4195
BsaBI GATnn'nnATC
Cuts at: 6 530 2070 4107 6
Size: 524 1540 2037 455
Fragments arranged by size:
2037 1540 524 455
BsaHI Gr'CG_yC
Cuts at: 130 1987 2235 2937 4433 130
Size: 1857 248 702 1496 253
Fragments arranged by size:
1857 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 188 1745 2266 2944 3218 3365 3776 188
Size: 1557 521 678 274 147 411 968
Fragments arranged by size:
1557 968 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 733 733
Size: 4556
BseYI C'CCAG_C
Cuts at: 650 819 1187 1981 2739 3316 3812 650
Size: 169 368 794 758 577 496 1394
Fragments arranged by size:
1394 794 758 577 496 368 169
BsiEI CG_ry'CG
Cuts at: 104 244 2098 2144 3352 104
Size: 140 1854 46 1208 1308
Fragments arranged by size:
1854 1308 1208 140 46
BsiHKAI G_wGCw'C
Cuts at: 455 728 1570 2348 2538 3330 455
Size: 273 842 778 190 792 1681
Fragments arranged by size:
1681 842 792 778 273 190
BsmI GAATG_Cn'
Cuts at: 1506 1506
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 455 728 1570 2181 2274 2348 2538 2600 3330
Size: 273 842 611 93 74 190 62 730
Cuts at: 3330 455
Size: 1681
Fragments arranged by size:
1681 842 730 611 273 190 93 74
62
BspEI T'CCGG_A
Cuts at: 188 3776 188
Size: 3588 968
BspHI T'CATG_A
Cuts at: 1004 3732 1004
Size: 2728 1828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 1606 1820 2122 2503 489
Size: 1117 214 302 381 2542
Fragments arranged by size:
2542 1117 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2848 2902 4386 4551 50
Size: 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 807 2468 807
Size: 1661 2895
BsrFI r'CCGG_y
Cuts at: 239 836 2554 2735 2944 239
Size: 597 1718 181 209 1851
Fragments arranged by size:
1851 1718 597 209 181
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 110 2917 110
Size: 2807 1749
BstEII G'GTnAC_C
Cuts at: 1354 1354
Size: 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 425 1085 425
Size: 660 3896
BstYI r'GATC_y
Cuts at: 1 525 1245 1406 1581 2048 2071 2406 2652
Size: 524 720 161 175 467 23 335 246
Cuts at: 2652 3664 3750 3762 4108 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 720 524 467 449 346 335 246
175 161 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 1799 1799
Size: 4556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 789 824 789
Size: 35 4521
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DraIII CAC_nnn'GTG
Cuts at: 1163 1163
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 241 2141 2315 2706 2733 4078 241
Size: 1900 174 391 27 1345 719
Fragments arranged by size:
1900 1345 719 391 174 27
EagI C'GGCC_G
Cuts at: 241 2141 241
Size: 1900 2656
EarI CTCTTCn'nnn_
Cuts at: 487 733 1255 2579 2789 487
Size: 246 522 1324 210 2254
Fragments arranged by size:
2254 1324 522 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 726 1568 726
Size: 842 3714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 1010 1234 1463 2381 2813 3560 3828 354
Size: 656 224 229 918 432 747 268 1082
Fragments arranged by size:
1082 918 747 656 432 268 229 224
EcoNI CCTnn'n_nnAGG
Cuts at: 157 300 600 157
Size: 143 300 4113
Fragments arranged by size:
4113 300 143
EcoO109I rG'GnC_Cy
Cuts at: 1695 1695
Size: 4556
EcoRI G'AATT_C
Cuts at: 1094 1560 1094
Size: 466 4090
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1071 1502 2575 1071
Size: 431 1073 3052
Fragments arranged by size:
3052 1073 431
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1990 2238 3260 4239 1990
Size: 248 1022 979 2307
Fragments arranged by size:
2307 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 952 984 1232 1237 1264 1269 1485 1517 2398
Size: 32 248 5 27 5 216 32 881
Cuts at: 2398 2428 2430 2460 952
Size: 30 2 30 3048
Fragments arranged by size:
3048 881 248 216 32 32 30 30
27 5 5 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
HindIII A'AGCT_T
Cuts at: 870 870
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1515 3227 3411 1515
Size: 1712 184 2660
Fragments arranged by size:
2660 1712 184
MscI TGG'CCA
Cuts at: 2317 2317
Size: 4556
MslI CAynn'nnrTG
Cuts at: 376 2545 2672 376
Size: 2169 127 2260
Fragments arranged by size:
2260 2169 127
MspA1I CmG'CkG
Cuts at: 1020 1667 1981 2341 3354 3599 1020
Size: 647 314 360 1013 245 1977
Fragments arranged by size:
1977 1013 647 360 314 245
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NcoI C'CATG_G
Cuts at: 125 125
Size: 4556
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NheI G'CTAG_C
Cuts at: 934 934
Size: 4556
NsiI A_TGCA'T
Cuts at: 185 740 185
Size: 555 4001
NspI r_CATG'y
Cuts at: 1465 1609 2109 3016 1465
Size: 144 500 907 3005
Fragments arranged by size:
3005 907 500 144
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PfoI T'CCnGG_A
Cuts at: 1211 1489 1211
Size: 278 4278
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PstI C_TGCA'G
Cuts at: 923 1603 2288 923
Size: 680 685 3191
Fragments arranged by size:
3191 685 680
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 1020 1981 2341 1020
Size: 961 360 3235
Fragments arranged by size:
3235 961 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 728 1570 728
Size: 842 3714
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 733 2579 2789 733
Size: 1846 210 2500
Fragments arranged by size:
2500 1846 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 1138 4073 1138
Size: 2935 1621
SfcI C'TryA_G
Cuts at: 33 919 1395 1599 2284 3277 3468 33
Size: 886 476 204 685 993 191 1121
Fragments arranged by size:
1121 993 886 685 476 204 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 661 661
Size: 4556
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 1711 2626 3118 3380 1711
Size: 915 492 262 2887
Fragments arranged by size:
2887 915 492 262
SnaBI TAC'GTA
Cuts at: 1548 1548
Size: 4556
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1465 1609 2109 1465
Size: 144 500 3912
Fragments arranged by size:
3912 500 144
StuI AGG'CCT
Cuts at: 664 664
Size: 4556
StyI C'CwwG_G
Cuts at: 125 1690 2044 125
Size: 1565 354 2637
Fragments arranged by size:
2637 1565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1179 1470 2139 2272 2731 2940 1179
Size: 291 669 133 459 209 2795
Fragments arranged by size:
2795 669 459 291 209 133
TatI w'GTAC_w
Cuts at: 287 1136 4071 287
Size: 849 2935 772
Fragments arranged by size:
2935 849 772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 434 466 1383 1415 4073 4105 434
Size: 32 917 32 2658 32 885
Fragments arranged by size:
2658 917 885 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2353 2353
Size: 4556
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 2051 1409
Size: 642 3914
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 1036 1036
Size: 4556
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SrfI SspI SwaI
XhoI ZraI