Restriction map for
pF1KSDA0025
(Circular) (Six-base) MAPSORT of: pF1KSDA0025.seq Check: 3586 from: 1 to: 4310
pF1KSDA0025 4310 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 762 762
Size: 4310
AccI GT'mk_AC
Cuts at: 257 1348 257
Size: 1091 3219
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 613 1178 2135 2567 3314 3582 581
Size: 32 565 957 432 747 268 1309
Fragments arranged by size:
1309 957 747 565 432 268 32
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 671 671
Size: 4310
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 514 546 514
Size: 32 4278
AlwNI CAG_nnn'CTG
Cuts at: 691 1615 3182 691
Size: 924 1567 1819
Fragments arranged by size:
1819 1567 924
ApaLI G'TGCA_C
Cuts at: 725 3080 725
Size: 2355 1955
ApoI r'AATT_y
Cuts at: 852 1314 3663 3919 852
Size: 462 2349 256 1243
Fragments arranged by size:
2349 1243 462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 145 262 1330 145
Size: 117 1068 3125
Fragments arranged by size:
3125 1068 117
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 152 175 1324 2354 152
Size: 23 1149 1030 2108
Fragments arranged by size:
2108 1149 1030 23
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 1166 1236 1166
Size: 70 4240
BciVI GTATCCnnnnn_n'
Cuts at: 482 973 2208 2969 4310 482
Size: 491 1235 761 1341 482
Fragments arranged by size:
1341 1235 761 491 482
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglI GCCn_nnn'nGGC
Cuts at: 427 574 427
Size: 147 4163
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 729 1935 2028 3084 729
Size: 1206 93 1056 1955
Fragments arranged by size:
1955 1206 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 501 2677 501
Size: 2176 2134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 743 860 1163 240
Size: 503 117 303 3387
Fragments arranged by size:
3387 503 303 117
Bpu10I CC'TnA_GC
Cuts at: 247 985 247
Size: 738 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 471 537 806 1486 2365 2857 3155 220
Size: 251 66 269 680 879 492 298 1375
Fragments arranged by size:
1375 879 680 492 298 269 251 66
BsaI GGTCTCn'nnnn_
Cuts at: 130 750 4014 130
Size: 620 3264 426
Fragments arranged by size:
3264 620 426
BsaAI yAC'GTr
Cuts at: 1302 1663 1302
Size: 361 3949
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 604 1499 2020 2698 2972 3119 3530 604
Size: 895 521 678 274 147 411 1384
Fragments arranged by size:
1384 895 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 961 991 961
Size: 30 4280
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 809 969 1085 457
Size: 352 160 116 3682
Fragments arranged by size:
3682 352 160 116
BseYI C'CCAG_C
Cuts at: 569 1284 1735 2493 3070 3566 569
Size: 715 451 758 577 496 1313
Fragments arranged by size:
1313 758 715 577 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 734
Size: 4310
BsiEI CG_ry'CG
Cuts at: 104 213 1852 1898 3106 104
Size: 109 1639 46 1208 1308
Fragments arranged by size:
1639 1308 1208 109 46
BsiHKAI G_wGCw'C
Cuts at: 729 1231 1324 2102 2292 3084 729
Size: 502 93 778 190 792 1955
Fragments arranged by size:
1955 792 778 502 190 93
BsmI GAATG_Cn'
Cuts at: 577 816 870 577
Size: 239 54 4017
Fragments arranged by size:
4017 239 54
Bsp1286I G_dGCh'C
Cuts at: 152 175 729 1231 1324 1935 2028 2102 2292
Size: 23 554 502 93 611 93 74 190
Cuts at: 2292 2354 3084 152
Size: 62 730 1378
Fragments arranged by size:
1378 730 611 554 502 190 93 93
74 62 23
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 762 1037 1360 1574 1876 2257 762
Size: 275 323 214 302 381 2815
Fragments arranged by size:
2815 381 323 302 275 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 390 2222 390
Size: 1832 2478
BsrFI r'CCGG_y
Cuts at: 1065 2308 2489 2698 1065
Size: 1243 181 209 2677
Fragments arranged by size:
2677 1243 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 369 691 1687 369
Size: 322 996 2992
Fragments arranged by size:
2992 996 322
BstBI TT'CG_AA
Cuts at: 110 2671 110
Size: 2561 1749
BstYI r'GATC_y
Cuts at: 1 1335 1802 1825 2160 2406 3418 3504 3516
Size: 1334 467 23 335 246 1012 86 12
Cuts at: 3516 3862 1
Size: 346 449
Fragments arranged by size:
1334 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 476 476
Size: 4310
BtgI C'CryG_G
Cuts at: 125 213 125
Size: 88 4222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1275 2260 2441 2604 1275
Size: 985 181 163 2981
Fragments arranged by size:
2981 985 181 163
BtsI GCAGTG_nn'
Cuts at: 689 1553 689
Size: 864 3446
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DraIII CAC_nnn'GTG
Cuts at: 724 874 724
Size: 150 4160
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 163 385 1271 2333 2543 163
Size: 222 886 1062 210 1930
Fragments arranged by size:
1930 1062 886 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 581 613 743 860 1163 1178 2135 2567
Size: 341 32 130 117 303 15 957 432
Cuts at: 2567 3314 3582 240
Size: 747 268 968
Fragments arranged by size:
968 957 747 432 341 303 268 130
117 32 15
EcoO109I rG'GnC_Cy
Cuts at: 592 1116 1279 1449 592
Size: 524 163 170 3453
Fragments arranged by size:
3453 524 170 163
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1272 2329 1272
Size: 1057 3253
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 186 1744 1992 3014 3993 186
Size: 1558 248 1022 979 503
Fragments arranged by size:
1558 1022 979 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2152 2182 2184 2214 2152
Size: 30 2 30 4248
Fragments arranged by size:
4248 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 899 2981 3165 296
Size: 603 2082 184 1441
Fragments arranged by size:
2082 1441 603 184
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 1003 2299 2426 1003
Size: 1296 127 2887
Fragments arranged by size:
2887 1296 127
MspA1I CmG'CkG
Cuts at: 215 787 1421 1735 2095 3108 3353 215
Size: 572 634 314 360 1013 245 1172
Fragments arranged by size:
1172 1013 634 572 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 125 125
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 367 675 1363 1863 2770 367
Size: 308 688 500 907 1907
Fragments arranged by size:
1907 907 688 500 308
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 588 1087 588
Size: 499 3811
PfoI T'CCnGG_A
Cuts at: 837 837
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 592 1116 592
Size: 524 3786
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 787 1735 2095 787
Size: 948 360 3002
Fragments arranged by size:
3002 948 360
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SacII CC_GC'GG
Cuts at: 216 216
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 163 2333 2543 163
Size: 2170 210 1930
Fragments arranged by size:
2170 1930 210
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 667 3827 667
Size: 3160 1150
SfcI C'TryA_G
Cuts at: 33 1353 2038 3031 3222 33
Size: 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 235 486 552 821 1465 2380 2872 3134 235
Size: 251 66 269 644 915 492 262 1411
Fragments arranged by size:
1411 915 644 492 269 262 251 66
SnaBI TAC'GTA
Cuts at: 1302 1302
Size: 4310
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
SspI AAT'ATT
Cuts at: 679 679
Size: 4310
StuI AGG'CCT
Cuts at: 558 587 558
Size: 29 4281
StyI C'CwwG_G
Cuts at: 125 422 582 1444 1798 125
Size: 297 160 862 354 2637
Fragments arranged by size:
2637 862 354 297 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 611 1031 1074 1893 2026 2485 2694 154
Size: 457 420 43 819 133 459 209 1770
Fragments arranged by size:
1770 819 459 457 420 209 133 43
TatI w'GTAC_w
Cuts at: 665 1191 3825 665
Size: 526 2634 1150
Fragments arranged by size:
2634 1150 526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1805 1805
Size: 4310
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
XmnI GAAnn'nnTTC
Cuts at: 515 515
Size: 4310
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SrfI
SwaI XhoI ZraI