Restriction map for
pF1KSDA0026
(Circular) (Six-base) MAPSORT of: pF1KSDA0026.seq Check: 2779 from: 1 to: 4001
pF1KSDA0026 4001 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 524 1826 2258 3005 3273 314
Size: 210 1302 432 747 268 1042
Fragments arranged by size:
1302 1042 747 432 268 210
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AhdI GACnn_n'nnGTC
Cuts at: 257 257
Size: 4001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 272 304 272
Size: 32 3969
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaI G_GGCC'C
Cuts at: 435 435
Size: 4001
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 727 921 1005 3354 3610 727
Size: 194 84 2349 256 1118
Fragments arranged by size:
2349 1118 256 194 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 434 1021 434
Size: 587 3414
BamHI G'GATC_C
Cuts at: 334 1026 334
Size: 692 3309
BanI G'GyrC_C
Cuts at: 1017 1431 1679 1714 1017
Size: 414 248 35 3304
Fragments arranged by size:
3304 414 248 35
BanII G_rGCy'C
Cuts at: 435 1015 2045 435
Size: 580 1030 2391
Fragments arranged by size:
2391 1030 580
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BbsI GAAGACnn'nnnn_
Cuts at: 256 352 373 256
Size: 96 21 3884
Fragments arranged by size:
3884 96 21
BbvCI CC'TCA_GC
Cuts at: 410 410
Size: 4001
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 1516 3553 1516
Size: 2037 1964
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 435 712 1626 1719 2775 435
Size: 277 914 93 1056 1661
Fragments arranged by size:
1661 1056 914 277 93
BmrI ACTGGGnnnn_n'
Cuts at: 543 735 1213 1294 1629 3773 543
Size: 192 478 81 335 2144 771
Fragments arranged by size:
2144 771 478 335 192 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 622
Size: 4001
Bpu10I CC'TnA_GC
Cuts at: 410 410
Size: 4001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 993 1354 993
Size: 361 3640
BsaBI GATnn'nnATC
Cuts at: 6 1515 3552 6
Size: 1509 2037 455
Fragments arranged by size:
2037 1509 455
BsaHI Gr'CG_yC
Cuts at: 1432 1680 2382 3878 1432
Size: 248 702 1496 1555
Fragments arranged by size:
1555 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1190 1711 2389 2663 2810 3221 1190
Size: 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 272 302 272
Size: 30 3971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 475 619 228
Size: 247 144 3610
Fragments arranged by size:
3610 247 144
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1543 1589 2797 104
Size: 1439 46 1208 1308
Fragments arranged by size:
1439 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 801 1015 1793 1983 2775 801
Size: 214 778 190 792 2027
Fragments arranged by size:
2027 792 778 214 190
Bsp1286I G_dGCh'C
Cuts at: 435 712 801 1015 1626 1719 1793 1983 2045
Size: 277 89 214 611 93 74 190 62
Cuts at: 2045 2775 435
Size: 730 1661
Fragments arranged by size:
1661 730 611 277 214 190 93 89
74 62
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1265 1567 1948 1051
Size: 214 302 381 3104
Fragments arranged by size:
3104 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2293 2347 3831 3996 50
Size: 2243 54 1484 165 55
Fragments arranged by size:
2243 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 842 1913 842
Size: 1071 2930
BsrFI r'CCGG_y
Cuts at: 1999 2180 2389 1999
Size: 181 209 3611
Fragments arranged by size:
3611 209 181
BssSI C'ACGA_G
Cuts at: 155 2272 2630 155
Size: 2117 358 1526
Fragments arranged by size:
2117 1526 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 110 2362 110
Size: 2252 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 299 793 299
Size: 494 3507
BstYI r'GATC_y
Cuts at: 1 334 1026 1493 1516 1851 2097 3109 3195
Size: 333 692 467 23 335 246 1012 86
Cuts at: 3195 3207 3553 1
Size: 12 346 449
Fragments arranged by size:
1012 692 467 449 346 335 333 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 240 240
Size: 4001
BtgI C'CryG_G
Cuts at: 517 517
Size: 4001
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1244
Size: 4001
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 1707 2565 1707
Size: 858 3143
EaeI y'GGCC_r
Cuts at: 1586 1760 2151 2178 3523 1586
Size: 174 391 27 1345 2064
Fragments arranged by size:
2064 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1586 1586
Size: 4001
EarI CTCTTCn'nnn_
Cuts at: 173 2024 2234 173
Size: 1851 210 1940
Fragments arranged by size:
1940 1851 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1013 1013
Size: 4001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 524 622 1826 2258 3005 3273 314
Size: 210 98 1204 432 747 268 1042
Fragments arranged by size:
1204 1042 747 432 268 210 98
EcoO109I rG'GnC_Cy
Cuts at: 233 431 737 1140 233
Size: 198 306 403 3094
Fragments arranged by size:
3094 403 306 198
EcoRI G'AATT_C
Cuts at: 921 1005 921
Size: 84 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 4001
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 1843 1873 1875 1905 243
Size: 32 1568 30 2 30 2339
Fragments arranged by size:
2339 1568 32 30 30 2
HincII GTy'rAC
Cuts at: 1040 1040
Size: 4001
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2856 2672
Size: 184 3817
MscI TGG'CCA
Cuts at: 1762 1762
Size: 4001
MslI CAynn'nnrTG
Cuts at: 770 1990 2117 770
Size: 1220 127 2654
Fragments arranged by size:
2654 1220 127
MspA1I CmG'CkG
Cuts at: 256 561 717 1112 1426 1786 2799 3044 256
Size: 305 156 395 314 360 1013 245 1213
Fragments arranged by size:
1213 1013 395 360 314 305 245 156
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 4001
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NcoI C'CATG_G
Cuts at: 517 517
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 4001
NspI r_CATG'y
Cuts at: 213 1054 1554 2461 213
Size: 841 500 907 1753
Fragments arranged by size:
1753 907 841 500
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PfoI T'CCnGG_A
Cuts at: 372 372
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 272 304 3140 3172 272
Size: 32 2836 32 1101
Fragments arranged by size:
2836 1101 32 32
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 4001
PstI C_TGCA'G
Cuts at: 174 318 1048 1733 174
Size: 144 730 685 2442
Fragments arranged by size:
2442 730 685 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 256 561 717 1426 1786 256
Size: 305 156 709 360 2471
Fragments arranged by size:
2471 709 360 305 156
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 1015 1015
Size: 4001
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 3518 3518
Size: 4001
SfcI C'TryA_G
Cuts at: 33 170 314 1044 1729 2722 2913 33
Size: 137 144 730 685 993 191 1121
Fragments arranged by size:
1121 993 730 685 191 144 137
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 436 1023 436
Size: 587 3414
SmlI C'TyrA_G
Cuts at: 1156 2071 2563 2825 1156
Size: 915 492 262 2332
Fragments arranged by size:
2332 915 492 262
SnaBI TAC'GTA
Cuts at: 993 993
Size: 4001
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
SrfI GCCC'GGGC
Cuts at: 436 436
Size: 4001
SspI AAT'ATT
Cuts at: 691 691
Size: 4001
StyI C'CwwG_G
Cuts at: 517 1135 1489 517
Size: 618 354 3029
Fragments arranged by size:
3029 618 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1584 1717 2176 2385 1584
Size: 133 459 209 3200
Fragments arranged by size:
3200 459 209 133
TatI w'GTAC_w
Cuts at: 3516 3516
Size: 4001
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 3969
Tth111I GACn'n_nGTC
Cuts at: 1798 1798
Size: 4001
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1496 1496
Size: 4001
XmaI C'CCGG_G
Cuts at: 434 1021 434
Size: 587 3414
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI StuI SwaI XhoI XmnI ZraI