Restriction map for
pF1KSDA0027
(Circular) (Six-base) MAPSORT of: pF1KSDA0027.seq Check: 2187 from: 1 to: 4268
pF1KSDA0027 4268 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 1222 2093 2525 3272 3540 249
Size: 973 871 432 747 268 977
Fragments arranged by size:
977 973 871 747 432 268
AflIII A'CryG_T
Cuts at: 347 496 1620 2724 3701 347
Size: 149 1124 1104 977 914
Fragments arranged by size:
1124 1104 977 914 149
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 952 1573 3140 952
Size: 621 1567 2080
Fragments arranged by size:
2080 1567 621
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 1272 3621 3877 1272
Size: 2349 256 1663
Fragments arranged by size:
2349 1663 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 321 701 757 1288 321
Size: 380 56 531 3301
Fragments arranged by size:
3301 531 380 56
BamHI G'GATC_C
Cuts at: 827 1293 827
Size: 466 3802
BanI G'GyrC_C
Cuts at: 1087 1284 1698 1946 1981 1087
Size: 197 414 248 35 3374
Fragments arranged by size:
3374 414 248 197 35
BanII G_rGCy'C
Cuts at: 655 1198 1282 2312 655
Size: 543 84 1030 2611
Fragments arranged by size:
2611 1030 543 84
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BbsI GAAGACnn'nnnn_
Cuts at: 276 276
Size: 4268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 216 234 250 268 216
Size: 18 16 18 4216
Fragments arranged by size:
4216 18 18 16
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 605 1788 605
Size: 1183 3085
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 1380 1380
Size: 4268
Bme1580I G_kGCm'C
Cuts at: 1090 1893 1986 3042 1090
Size: 803 93 1056 2316
Fragments arranged by size:
2316 1056 803 93
BmgBI CAC'GTC
Cuts at: 278 929 278
Size: 651 3617
BmrI ACTGGGnnnn_n'
Cuts at: 1248 1480 1561 1896 4040 1248
Size: 232 81 335 2144 1476
Fragments arranged by size:
2144 1476 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 411 601 1248 199
Size: 212 190 647 3219
Fragments arranged by size:
3219 647 212 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 497 1260 1621 497
Size: 763 361 3144
Fragments arranged by size:
3144 763 361
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 1699 1947 2649 4145 1699
Size: 248 702 1496 1822
Fragments arranged by size:
1822 1496 702 248
BsaWI w'CCGG_w
Cuts at: 164 1457 1978 2656 2930 3077 3488 164
Size: 1293 521 678 274 147 411 944
Fragments arranged by size:
1293 944 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 643 163
Size: 480 3788
BseYI C'CCAG_C
Cuts at: 883 1177 1693 2451 3028 3524 883
Size: 294 516 758 577 496 1627
Fragments arranged by size:
1627 758 577 516 496 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 4268
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1061 1282 2060 2250 3042 1061
Size: 221 778 190 792 2287
Fragments arranged by size:
2287 792 778 221 190
BsmBI CGTCTCn'nnnn_
Cuts at: 429 935 429
Size: 506 3762
Bsp1286I G_dGCh'C
Cuts at: 655 1061 1090 1198 1282 1893 1986 2060 2250
Size: 406 29 108 84 611 93 74 190
Cuts at: 2250 2312 3042 655
Size: 62 730 1881
Fragments arranged by size:
1881 730 611 406 190 108 93 84
74 62 29
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 974 3444 974
Size: 2470 1798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1318 1532 1834 2215 242
Size: 1076 214 302 381 2295
Fragments arranged by size:
2295 1076 381 302 214
BsrBI CCG'CTC
Cuts at: 50 183 2560 2614 4098 4263 50
Size: 133 2377 54 1484 165 55
Fragments arranged by size:
2377 1484 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 767 854 2180 767
Size: 87 1326 2855
Fragments arranged by size:
2855 1326 87
BsrFI r'CCGG_y
Cuts at: 188 1072 2266 2447 2656 188
Size: 884 1194 181 209 1800
Fragments arranged by size:
1800 1194 884 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 110 2629 110
Size: 2519 1749
BstEII G'GTnAC_C
Cuts at: 315 315
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 678 867 678
Size: 189 4079
BstYI r'GATC_y
Cuts at: 1 827 1293 1760 1783 2118 2364 3376 3462
Size: 826 466 467 23 335 246 1012 86
Cuts at: 3462 3474 3820 1
Size: 12 346 449
Fragments arranged by size:
1012 826 467 466 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 599 599
Size: 4268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 594 2218 2399 2562 594
Size: 1624 181 163 2300
Fragments arranged by size:
2300 1624 181 163
BtsI GCAGTG_nn'
Cuts at: 1511 1511
Size: 4268
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DraIII CAC_nnn'GTG
Cuts at: 497 497
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 596 1187 1853 2027 2418 2445 3790 596
Size: 591 666 174 391 27 1345 1074
Fragments arranged by size:
1345 1074 666 591 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 241 2291 2501 241
Size: 2050 210 2008
Fragments arranged by size:
2050 2008 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 2786 2932 4078 2476
Size: 310 146 1146 2666
Fragments arranged by size:
2666 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 249 411 601 1222 1248 2093 2525 3272
Size: 50 162 190 621 26 845 432 747
Cuts at: 3272 3540 199
Size: 268 927
Fragments arranged by size:
927 845 747 621 432 268 190 162
50 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1066 1066
Size: 4268
EcoO109I rG'GnC_Cy
Cuts at: 705 903 1407 705
Size: 198 504 3566
Fragments arranged by size:
3566 504 198
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
EcoRV GAT'ATC
Cuts at: 958 958
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FseI GG_CCGG'CC
Cuts at: 1076 1076
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 671 1173 1702 1950 2972 3951 671
Size: 502 529 248 1022 979 988
Fragments arranged by size:
1022 988 979 529 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2110 2140 2142 2172 2110
Size: 30 2 30 4206
Fragments arranged by size:
4206 30 30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MfeI C'AATT_G
Cuts at: 407 407
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3123 2939
Size: 184 4084
MscI TGG'CCA
Cuts at: 1189 2029 1189
Size: 840 3428
MslI CAynn'nnrTG
Cuts at: 2257 2384 2257
Size: 127 4141
MspA1I CmG'CkG
Cuts at: 240 936 1379 1693 2053 3066 3311 240
Size: 696 443 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 696 443 360 314 245
NaeI GCC'GGC
Cuts at: 190 1074 2449 190
Size: 884 1375 2009
Fragments arranged by size:
2009 1375 884
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NgoMIV G'CCGG_C
Cuts at: 188 1072 2447 188
Size: 884 1375 2009
Fragments arranged by size:
2009 1375 884
NspI r_CATG'y
Cuts at: 1321 1821 2728 1321
Size: 500 907 2861
Fragments arranged by size:
2861 907 500
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PfoI T'CCnGG_A
Cuts at: 427 427
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 705 903 705
Size: 198 4070
PshAI GACnn'nnGTC
Cuts at: 280 280
Size: 4268
PstI C_TGCA'G
Cuts at: 239 387 1070 1133 1315 2000 239
Size: 148 683 63 182 685 2507
Fragments arranged by size:
2507 685 683 182 148 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 240 1693 2053 240
Size: 1453 360 2455
Fragments arranged by size:
2455 1453 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 235 383 1066 1129 1311 1996 2989 3180
Size: 202 148 683 63 182 685 993 191
Cuts at: 3180 33
Size: 1121
Fragments arranged by size:
1121 993 685 683 202 191 182 148
63
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 2830 3092 1423
Size: 915 492 262 2599
Fragments arranged by size:
2599 915 492 262
SnaBI TAC'GTA
Cuts at: 1260 1260
Size: 4268
SpeI A'CTAG_T
Cuts at: 67 522 67
Size: 455 3813
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 1402 1756 1402
Size: 354 3914
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 1851 1984 2443 2652 1165
Size: 686 133 459 209 2781
Fragments arranged by size:
2781 686 459 209 133
TatI w'GTAC_w
Cuts at: 3783 3783
Size: 4268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1763 891
Size: 872 3396
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
XmnI GAAnn'nnTTC
Cuts at: 349 1207 349
Size: 858 3410
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI