Restriction map for
pF1KSDA0031
(Circular) (Six-base) MAPSORT of: pF1KSDA0031.seq Check: 1345 from: 1 to: 6053
pF1KSDA0031 6053 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2573 3091 2573
Size: 518 5535
Acc65I G'GTAC_C
Cuts at: 1733 3069 1733
Size: 1336 4717
AclI AA'CG_TT
Cuts at: 5893 5893
Size: 6053
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1369 3878 4310 5057 5325 946
Size: 423 2509 432 747 268 1674
Fragments arranged by size:
2509 1674 747 432 423 268
AfeI AGC'GCT
Cuts at: 604 604
Size: 6053
AflIII A'CryG_T
Cuts at: 553 747 1421 3405 4509 5486 553
Size: 194 674 1984 1104 977 1120
Fragments arranged by size:
1984 1120 1104 977 674 194
AgeI A'CCGG_T
Cuts at: 1486 4441 1486
Size: 2955 3098
AleI CACnn'nnGTG
Cuts at: 679 1984 679
Size: 1305 4748
AlwNI CAG_nnn'CTG
Cuts at: 886 1073 3358 4925 886
Size: 187 2285 1567 2014
Fragments arranged by size:
2285 2014 1567 187
ApaI G_GGCC'C
Cuts at: 2458 2458
Size: 6053
ApaLI G'TGCA_C
Cuts at: 4823 4823
Size: 6053
ApoI r'AATT_y
Cuts at: 427 753 1138 2920 2977 3057 5406 5662 427
Size: 326 385 1782 57 80 2349 256 818
Fragments arranged by size:
2349 1782 818 385 326 256 80 57
AseI AT'TA_AT
Cuts at: 20 988 1127 20
Size: 968 139 4946
Fragments arranged by size:
4946 968 139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
AvaI C'yCGr_G
Cuts at: 1829 2915 3073 1829
Size: 1086 158 4809
Fragments arranged by size:
4809 1086 158
BamHI G'GATC_C
Cuts at: 3078 3078
Size: 6053
BanI G'GyrC_C
Cuts at: 1456 1621 1733 3069 3483 3731 3766 1456
Size: 165 112 1336 414 248 35 3743
Fragments arranged by size:
3743 1336 414 248 165 112 35
BanII G_rGCy'C
Cuts at: 509 1090 2030 2458 2525 3067 4097 509
Size: 581 940 428 67 542 1030 2465
Fragments arranged by size:
2465 1030 940 581 542 428 67
BbeI G_GCGC'C
Cuts at: 1460 3487 3735 1460
Size: 2027 248 3778
Fragments arranged by size:
3778 2027 248
BbsI GAAGACnn'nnnn_
Cuts at: 383 1691 1813 2817 383
Size: 1308 122 1004 3619
Fragments arranged by size:
3619 1308 1004 122
BbvCI CC'TCA_GC
Cuts at: 821 821
Size: 6053
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2964 2998 2964
Size: 34 6019
BciVI GTATCCnnnnn_n'
Cuts at: 123 3951 4712 6053 123
Size: 3828 761 1341 123
Fragments arranged by size:
3828 1341 761 123
BclI T'GATC_A
Cuts at: 851 1791 2927 3573 851
Size: 940 1136 646 3331
Fragments arranged by size:
3331 1136 940 646
BfrBI ATG'CAT
Cuts at: 2049 2049
Size: 6053
BglII A'GATC_T
Cuts at: 219 615 1100 3568 5605 219
Size: 396 485 2468 2037 667
Fragments arranged by size:
2468 2037 667 485 396
BlpI GC'TnA_GC
Cuts at: 2192 3165 2192
Size: 973 5080
Bme1580I G_kGCm'C
Cuts at: 2020 2458 2693 3678 3771 4827 2020
Size: 438 235 985 93 1056 3246
Fragments arranged by size:
3246 1056 985 438 235 93
BmgBI CAC'GTC
Cuts at: 1287 1287
Size: 6053
BmrI ACTGGGnnnn_n'
Cuts at: 1056 1663 1779 2030 2092 3265 3346 3681 5825
Size: 607 116 251 62 1173 81 335 2144
Cuts at: 5825 1056
Size: 1284
Fragments arranged by size:
2144 1284 1173 607 335 251 116 81
62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1704 4420 1704
Size: 2716 3337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 940 1038 1693 725
Size: 215 98 655 5085
Fragments arranged by size:
5085 655 215 98
Bpu10I CC'TnA_GC
Cuts at: 821 1372 821
Size: 551 5502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 2221 3229 4108 4600 4898 2125
Size: 96 1008 879 492 298 3280
Fragments arranged by size:
3280 1008 879 492 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 205 352 5757 205
Size: 147 5405 501
Fragments arranged by size:
5405 501 147
BsaAI yAC'GTr
Cuts at: 2249 2694 3045 3406 2249
Size: 445 351 361 4896
Fragments arranged by size:
4896 445 361 351
BsaBI GATnn'nnATC
Cuts at: 6 2080 2297 3567 5604 6
Size: 2074 217 1270 2037 455
Fragments arranged by size:
2074 2037 1270 455 217
BsaHI Gr'CG_yC
Cuts at: 1457 3484 3732 4434 5930 1457
Size: 2027 248 702 1496 1580
Fragments arranged by size:
2027 1580 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1486 2786 3242 3763 4441 4715 4862 5273 1486
Size: 1300 456 521 678 274 147 411 2266
Fragments arranged by size:
2266 1300 678 521 456 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 349 1357 1855 2005 2588 2894 349
Size: 1008 498 150 583 306 3508
Fragments arranged by size:
3508 1008 583 498 306 150
BseYI C'CCAG_C
Cuts at: 1083 1633 2265 3478 4236 4813 5309 1083
Size: 550 632 1213 758 577 496 1827
Fragments arranged by size:
1827 1213 758 632 577 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1450 2512 886
Size: 564 1062 4427
Fragments arranged by size:
4427 1062 564
BsiEI CG_ry'CG
Cuts at: 104 3595 3641 4849 104
Size: 3491 46 1208 1308
Fragments arranged by size:
3491 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 509 1478 2030 3029 3067 3845 4035 4827 509
Size: 969 552 999 38 778 190 792 1735
Fragments arranged by size:
1735 999 969 792 778 552 190 38
BsmBI CGTCTCn'nnnn_
Cuts at: 2110 2658 2110
Size: 548 5505
Bsp1286I G_dGCh'C
Cuts at: 509 1090 1478 2020 2030 2458 2525 2693 3029
Size: 581 388 542 10 428 67 168 336
Cuts at: 3029 3067 3678 3771 3845 4035 4097 4827 509
Size: 38 611 93 74 190 62 730 1735
Fragments arranged by size:
1735 730 611 581 542 428 388 336
190 168 93 74 67 62 38 10
BspEI T'CCGG_A
Cuts at: 2786 5273 2786
Size: 2487 3566
BspHI T'CATG_A
Cuts at: 267 2924 5229 267
Size: 2657 2305 1091
Fragments arranged by size:
2657 2305 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3103 3317 3619 4000 3103
Size: 214 302 381 5156
Fragments arranged by size:
5156 381 302 214
BsrBI CCG'CTC
Cuts at: 50 846 4345 4399 5883 6048 50
Size: 796 3499 54 1484 165 55
Fragments arranged by size:
3499 1484 796 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1842 3965 1842
Size: 2123 3930
BsrFI r'CCGG_y
Cuts at: 908 1486 4051 4232 4441 908
Size: 578 2565 181 209 2520
Fragments arranged by size:
2565 2520 578 209 181
BsrGI T'GTAC_A
Cuts at: 1568 2729 1568
Size: 1161 4892
BssSI C'ACGA_G
Cuts at: 4324 4682 4324
Size: 358 5695
BstAPI GCAn_nnn'nTGC
Cuts at: 864 2055 2844 3430 864
Size: 1191 789 586 3487
Fragments arranged by size:
3487 1191 789 586
BstBI TT'CG_AA
Cuts at: 110 4414 110
Size: 4304 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1986 2358 1986
Size: 372 5681
BstYI r'GATC_y
Cuts at: 1 219 486 615 1100 1262 1718 2298 2495
Size: 218 267 129 485 162 456 580 197
Cuts at: 2495 3078 3545 3568 3903 4149 5161 5247 5259
Size: 583 467 23 335 246 1012 86 12
Cuts at: 5259 5605 1
Size: 346 449
Fragments arranged by size:
1012 583 580 485 467 456 449 346
335 267 246 218 197 162 129 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 350 350
Size: 6053
BtgI C'CryG_G
Cuts at: 125 543 1303 1703 125
Size: 418 760 400 4475
Fragments arranged by size:
4475 760 418 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4003 4184 4347 4003
Size: 181 163 5709
Fragments arranged by size:
5709 181 163
BtsI GCAGTG_nn'
Cuts at: 3296 3296
Size: 6053
DraI TTT'AAA
Cuts at: 3052 3052
Size: 6053
DraIII CAC_nnn'GTG
Cuts at: 890 969 890
Size: 79 5974
DrdI GACnn_nn'nnGTC
Cuts at: 2101 3759 4617 2101
Size: 1658 858 3537
Fragments arranged by size:
3537 1658 858
EaeI y'GGCC_r
Cuts at: 1605 1727 2675 2753 3638 3812 4203 4230 5575
Size: 122 948 78 885 174 391 27 1345
Cuts at: 5575 1605
Size: 2083
Fragments arranged by size:
2083 1345 948 885 391 174 122 78
27
EagI C'GGCC_G
Cuts at: 3638 3638
Size: 6053
EarI CTCTTCn'nnn_
Cuts at: 643 727 2402 4076 4286 643
Size: 84 1675 1674 210 2410
Fragments arranged by size:
2410 1675 1674 210 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 499 1351 2869 4261 4571 4717 5863 499
Size: 852 1518 1392 310 146 1146 689
Fragments arranged by size:
1518 1392 1146 852 689 310 146
EcoICRI GAG'CTC
Cuts at: 507 2028 3065 507
Size: 1521 1037 3495
Fragments arranged by size:
3495 1521 1037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 940 946 1038 1369 1693 3878 4310 5057
Size: 215 6 92 331 324 2185 432 747
Cuts at: 5057 5325 725
Size: 268 1453
Fragments arranged by size:
2185 1453 747 432 331 324 268 215
92 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1270 1336 1371 1510 1948 2215 1270
Size: 66 35 139 438 267 5108
Fragments arranged by size:
5108 438 267 139 66 35
EcoO109I rG'GnC_Cy
Cuts at: 1203 2208 2454 2455 2637 2949 3192 1203
Size: 1005 246 1 182 312 243 4064
Fragments arranged by size:
4064 1005 312 246 243 182 1
EcoRI G'AATT_C
Cuts at: 2920 3057 2920
Size: 137 5916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 6053
FspI TGC'GCA
Cuts at: 784 1948 3834 784
Size: 1164 1886 3003
Fragments arranged by size:
3003 1886 1164
HaeII r_GCGC'y
Cuts at: 606 1460 3487 3735 4757 5736 606
Size: 854 2027 248 1022 979 923
Fragments arranged by size:
2027 1022 979 923 854 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 2638 2670 3895 3925 3927 3957 204
Size: 32 2402 32 1225 30 2 30 2300
Fragments arranged by size:
2402 2300 1225 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1911 1956 3092 1911
Size: 45 1136 4872
Fragments arranged by size:
4872 1136 45
HindIII A'AGCT_T
Cuts at: 2741 2741
Size: 6053
KasI G'GCGC_C
Cuts at: 1456 3483 3731 1456
Size: 2027 248 3778
Fragments arranged by size:
3778 2027 248
KpnI G_GTAC'C
Cuts at: 1737 3073 1737
Size: 1336 4717
MfeI C'AATT_G
Cuts at: 1799 1799
Size: 6053
MluI A'CGCG_T
Cuts at: 5486 5486
Size: 6053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 961 1526 1881 4724 4908 961
Size: 565 355 2843 184 2106
Fragments arranged by size:
2843 2106 565 355 184
MscI TGG'CCA
Cuts at: 1729 2677 3814 1729
Size: 948 1137 3968
Fragments arranged by size:
3968 1137 948
MslI CAynn'nnrTG
Cuts at: 272 436 679 1426 1744 1984 2086 2359 4042
Size: 164 243 747 318 240 102 273 1683
Cuts at: 4042 4169 272
Size: 127 2156
Fragments arranged by size:
2156 1683 747 318 273 243 240 164
127 102
MspA1I CmG'CkG
Cuts at: 775 3164 3478 3838 4851 5096 775
Size: 2389 314 360 1013 245 1732
Fragments arranged by size:
2389 1732 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4234 4234
Size: 6053
NarI GG'CG_CC
Cuts at: 1457 3484 3732 1457
Size: 2027 248 3778
Fragments arranged by size:
3778 2027 248
NcoI C'CATG_G
Cuts at: 125 543 125
Size: 418 5635
NgoMIV G'CCGG_C
Cuts at: 4232 4232
Size: 6053
NsiI A_TGCA'T
Cuts at: 2051 2051
Size: 6053
NspI r_CATG'y
Cuts at: 557 751 862 999 1425 2008 3106 3606 4513
Size: 194 111 137 426 583 1098 500 907
Cuts at: 4513 557
Size: 2097
Fragments arranged by size:
2097 1098 907 583 500 426 194 137
111
PasI CC'CwG_GG
Cuts at: 283 2799 283
Size: 2516 3537
PciI A'CATG_T
Cuts at: 553 747 1421 4509 553
Size: 194 674 3088 2097
Fragments arranged by size:
3088 2097 674 194
PflMI CCAn_nnn'nTGG
Cuts at: 969 969
Size: 6053
PfoI T'CCnGG_A
Cuts at: 741 917 2498 741
Size: 176 1581 4296
Fragments arranged by size:
4296 1581 176
PmeI GTTT'AAAC
Cuts at: 3052 3052
Size: 6053
PmlI CAC'GTG
Cuts at: 2694 2694
Size: 6053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 5192
Size: 32 6021
PshAI GACnn'nnGTC
Cuts at: 688 688
Size: 6053
PsiI TTA'TAA
Cuts at: 1259 1259
Size: 6053
PspOMI G'GGCC_C
Cuts at: 2454 2454
Size: 6053
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 6021
PstI C_TGCA'G
Cuts at: 2646 2661 3100 3785 2646
Size: 15 439 685 4914
Fragments arranged by size:
4914 685 439 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6053
PvuII CAG'CTG
Cuts at: 775 3478 3838 775
Size: 2703 360 2990
Fragments arranged by size:
2990 2703 360
RsrII CG'GwC_CG
Cuts at: 4248 4248
Size: 6053
SacI G_AGCT'C
Cuts at: 509 2030 3067 509
Size: 1521 1037 3495
Fragments arranged by size:
3495 1521 1037
SalI G'TCGA_C
Cuts at: 3090 3090
Size: 6053
SapI GCTCTTCn'nnn_
Cuts at: 2402 4076 4286 2402
Size: 1674 210 4169
Fragments arranged by size:
4169 1674 210
SbfI CC_TGCA'GG
Cuts at: 3100 3100
Size: 6053
ScaI AGT'ACT
Cuts at: 319 5570 319
Size: 5251 802
SfcI C'TryA_G
Cuts at: 33 2642 2657 3096 3781 4774 4965 33
Size: 2609 15 439 685 993 191 1121
Fragments arranged by size:
2609 1121 993 685 439 191 15
SfoI GGC'GCC
Cuts at: 1458 3485 3733 1458
Size: 2027 248 3778
Fragments arranged by size:
3778 2027 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
SgrAI Cr'CCGG_yG
Cuts at: 1486 1486
Size: 6053
SmaI CCC'GGG
Cuts at: 2917 3075 2917
Size: 158 5895
SmlI C'TyrA_G
Cuts at: 2140 2200 3208 4123 4615 4877 2140
Size: 60 1008 915 492 262 3316
Fragments arranged by size:
3316 1008 915 492 262 60
SnaBI TAC'GTA
Cuts at: 3045 3045
Size: 6053
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6053
SphI G_CATG'C
Cuts at: 862 3106 3606 862
Size: 2244 500 3309
Fragments arranged by size:
3309 2244 500
StyI C'CwwG_G
Cuts at: 125 543 1982 2885 3187 3541 125
Size: 418 1439 903 302 354 2637
Fragments arranged by size:
2637 1439 903 418 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2724 2812 3636 3769 4228 4437 2724
Size: 88 824 133 459 209 4340
Fragments arranged by size:
4340 824 459 209 133 88
TatI w'GTAC_w
Cuts at: 317 1064 1568 2066 2729 5568 317
Size: 747 504 498 663 2839 802
Fragments arranged by size:
2839 802 747 663 504 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5570 5602 5570
Size: 32 6021
Tth111I GACn'n_nGTC
Cuts at: 3850 3850
Size: 6053
XbaI T'CTAG_A
Cuts at: 3084 3084
Size: 6053
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 3548 2358
Size: 1190 4863
XmaI C'CCGG_G
Cuts at: 2915 3073 2915
Size: 158 5895
XmnI GAAnn'nnTTC
Cuts at: 2474 2474
Size: 6053
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BglI BmtI BsaXI BsiWI BsmI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI PacI PpuMI PspXI SacII SanDI
SexAI SfiI SrfI SspI StuI SwaI XhoI ZraI