Restriction map for
pF1KSDA0032
(Circular) (Six-base) MAPSORT of: pF1KSDA0032.seq Check: 2089 from: 1 to: 6287
pF1KSDA0032 6287 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2139 2139
Size: 6287
AccI GT'mk_AC
Cuts at: 3325 3325
Size: 6287
Acc65I G'GTAC_C
Cuts at: 3303 3303
Size: 6287
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1278 2645 2891 4112 4544 5291 5559 1278
Size: 1367 246 1221 432 747 268 2006
Fragments arranged by size:
2006 1367 1221 747 432 268 246
AflIII A'CryG_T
Cuts at: 295 2462 2464 3639 4743 5720 295
Size: 2167 2 1175 1104 977 862
Fragments arranged by size:
2167 1175 1104 977 862 2
AgeI A'CCGG_T
Cuts at: 4675 4675
Size: 6287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 6287
AlwNI CAG_nnn'CTG
Cuts at: 754 2134 3592 5159 754
Size: 1380 1458 1567 1882
Fragments arranged by size:
1882 1567 1458 1380
ApaLI G'TGCA_C
Cuts at: 5057 5057
Size: 6287
ApoI r'AATT_y
Cuts at: 2371 2788 3291 5640 5896 2371
Size: 417 503 2349 256 2762
Fragments arranged by size:
2762 2349 503 417 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 1187 2659 3307 1187
Size: 1472 648 4167
Fragments arranged by size:
4167 1472 648
BamHI G'GATC_C
Cuts at: 692 3312 692
Size: 2620 3667
BanI G'GyrC_C
Cuts at: 3303 3717 3965 4000 3303
Size: 414 248 35 5590
Fragments arranged by size:
5590 414 248 35
BanII G_rGCy'C
Cuts at: 200 366 726 797 2897 3301 4331 200
Size: 166 360 71 2100 404 1030 2156
Fragments arranged by size:
2156 2100 1030 404 360 166 71
BbeI G_GCGC'C
Cuts at: 3721 3969 3721
Size: 248 6039
BbsI GAAGACnn'nnnn_
Cuts at: 2006 2175 2606 2808 2006
Size: 169 431 202 5485
Fragments arranged by size:
5485 431 202 169
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2055 4185 4946 6287 1728
Size: 327 2130 761 1341 1728
Fragments arranged by size:
2130 1728 1341 761 327
BclI T'GATC_A
Cuts at: 3807 3807
Size: 6287
BfrBI ATG'CAT
Cuts at: 1078 1078
Size: 6287
BglI GCCn_nnn'nGGC
Cuts at: 721 721
Size: 6287
BglII A'GATC_T
Cuts at: 1223 3802 5839 1223
Size: 2579 2037 1671
Fragments arranged by size:
2579 2037 1671
BlpI GC'TnA_GC
Cuts at: 194 2281 3399 194
Size: 2087 1118 3082
Fragments arranged by size:
3082 2087 1118
Bme1580I G_kGCm'C
Cuts at: 509 1118 1138 3231 3912 4005 5061 509
Size: 609 20 2093 681 93 1056 1735
Fragments arranged by size:
2093 1735 1056 681 609 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 331 1121 1167 1596 2958 3499 3580 3915 6059
Size: 790 46 429 1362 541 81 335 2144
Cuts at: 6059 331
Size: 559
Fragments arranged by size:
2144 1362 790 559 541 429 335 81
46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 4654 669
Size: 3985 2302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1253 1657 2221 706
Size: 547 404 564 4772
Fragments arranged by size:
4772 564 547 404
Bpu10I CC'TnA_GC
Cuts at: 636 704 636
Size: 68 6219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2911 3463 4342 4834 5132 2911
Size: 552 879 492 298 4066
Fragments arranged by size:
4066 879 552 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 152 1232 1775 5991 152
Size: 1080 543 4216 448
Fragments arranged by size:
4216 1080 543 448
BsaAI yAC'GTr
Cuts at: 2465 3279 3640 2465
Size: 814 361 5112
Fragments arranged by size:
5112 814 361
BsaBI GATnn'nnATC
Cuts at: 6 2441 3097 3127 3801 5838 6
Size: 2435 656 30 674 2037 455
Fragments arranged by size:
2435 2037 674 656 455 30
BsaHI Gr'CG_yC
Cuts at: 3718 3966 4668 6164 3718
Size: 248 702 1496 3841
Fragments arranged by size:
3841 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3476 3997 4675 4949 5096 5507 3476
Size: 521 678 274 147 411 4256
Fragments arranged by size:
4256 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 450 480 2744 2774 450
Size: 30 2264 30 3963
Fragments arranged by size:
3963 2264 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1250 2689 3166 1250
Size: 1439 477 4371
Fragments arranged by size:
4371 1439 477
BseYI C'CCAG_C
Cuts at: 764 1744 3712 4470 5047 5543 764
Size: 980 1968 758 577 496 1508
Fragments arranged by size:
1968 1508 980 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 1841 473
Size: 1368 4919
BsiEI CG_ry'CG
Cuts at: 104 3090 3829 3875 5083 104
Size: 2986 739 46 1208 1308
Fragments arranged by size:
2986 1308 1208 739 46
BsiHKAI G_wGCw'C
Cuts at: 366 2480 2897 3301 4079 4269 5061 366
Size: 2114 417 404 778 190 792 1592
Fragments arranged by size:
2114 1592 792 778 417 404 190
BsmI GAATG_Cn'
Cuts at: 2285 2285
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 200 366 509 726 797 1118 1138 2480 2897
Size: 166 143 217 71 321 20 1342 417
Cuts at: 2897 3231 3301 3912 4005 4079 4269 4331 5061
Size: 334 70 611 93 74 190 62 730
Cuts at: 5061 200
Size: 1426
Fragments arranged by size:
1426 1342 730 611 417 334 321 217
190 166 143 93 74 71 70 62
20
BspEI T'CCGG_A
Cuts at: 5507 5507
Size: 6287
BspHI T'CATG_A
Cuts at: 479 1793 2832 3033 5463 479
Size: 1314 1039 201 2430 1303
Fragments arranged by size:
2430 1314 1303 1039 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 185 2139 2180 3337 3551 3853 4234 185
Size: 1954 41 1157 214 302 381 2238
Fragments arranged by size:
2238 1954 1157 381 302 214 41
BsrBI CCG'CTC
Cuts at: 50 4579 4633 6117 6282 50
Size: 4529 54 1484 165 55
Fragments arranged by size:
4529 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 567 4199 567
Size: 3632 2655
BsrFI r'CCGG_y
Cuts at: 2353 3162 4285 4466 4675 2353
Size: 809 1123 181 209 3965
Fragments arranged by size:
3965 1123 809 209 181
BssSI C'ACGA_G
Cuts at: 1984 4558 4916 1984
Size: 2574 358 3355
Fragments arranged by size:
3355 2574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2350 3664 2350
Size: 1314 4973
BstBI TT'CG_AA
Cuts at: 110 4648 110
Size: 4538 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 208 1986 208
Size: 1778 4509
BstYI r'GATC_y
Cuts at: 1 692 1223 3312 3779 3802 4137 4383 5395
Size: 691 531 2089 467 23 335 246 1012
Cuts at: 5395 5481 5493 5839 1
Size: 86 12 346 449
Fragments arranged by size:
2089 1012 691 531 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 621 621
Size: 6287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 509 2351 3530 509
Size: 1842 1179 3266
Fragments arranged by size:
3266 1842 1179
DraI TTT'AAA
Cuts at: 1203 1473 3286 1203
Size: 270 1813 4204
Fragments arranged by size:
4204 1813 270
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 229 3993 4851 229
Size: 3764 858 1665
Fragments arranged by size:
3764 1665 858
EaeI y'GGCC_r
Cuts at: 591 1173 1718 2213 2993 3110 3872 4046 4437
Size: 582 545 495 780 117 762 174 391
Cuts at: 4437 4464 5809 591
Size: 27 1345 1069
Fragments arranged by size:
1345 1069 780 762 582 545 495 391
174 117 27
EagI C'GGCC_G
Cuts at: 3872 3872
Size: 6287
EarI CTCTTCn'nnn_
Cuts at: 978 2902 2957 4310 4520 978
Size: 1924 55 1353 210 2745
Fragments arranged by size:
2745 1924 1353 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4495 4805 4951 6097 4495
Size: 310 146 1146 4685
Fragments arranged by size:
4685 1146 310 146
EcoICRI GAG'CTC
Cuts at: 364 2895 3299 364
Size: 2531 404 3352
Fragments arranged by size:
3352 2531 404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1253 1278 1657 2221 2645 2891 4112 4544
Size: 547 25 379 564 424 246 1221 432
Cuts at: 4544 5291 5559 706
Size: 747 268 1434
Fragments arranged by size:
1434 1221 747 564 547 432 424 379
268 246 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1672 1672
Size: 6287
EcoO109I rG'GnC_Cy
Cuts at: 680 3243 3426 680
Size: 2563 183 3541
Fragments arranged by size:
3541 2563 183
EcoRI G'AATT_C
Cuts at: 3291 3291
Size: 6287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1242 1872 2610 3084 4306 1242
Size: 630 738 474 1222 3223
Fragments arranged by size:
3223 1222 738 630 474
FspI TGC'GCA
Cuts at: 4068 4068
Size: 6287
HaeII r_GCGC'y
Cuts at: 929 3721 3969 4991 5970 929
Size: 2792 248 1022 979 1246
Fragments arranged by size:
2792 1246 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 450 482 802 834 1349 1381 1492 1524 1965
Size: 32 320 32 515 32 111 32 441
Cuts at: 1965 1997 2504 2536 4129 4159 4161 4191 450
Size: 32 507 32 1593 30 2 30 2546
Fragments arranged by size:
2546 1593 515 507 441 320 111 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 975 1290 3326 975
Size: 315 2036 3936
Fragments arranged by size:
3936 2036 315
HpaI GTT'AAC
Cuts at: 975 975
Size: 6287
KasI G'GCGC_C
Cuts at: 3717 3965 3717
Size: 248 6039
KpnI G_GTAC'C
Cuts at: 3307 3307
Size: 6287
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2241 4958 5142 1411
Size: 830 2717 184 2556
Fragments arranged by size:
2717 2556 830 184
MscI TGG'CCA
Cuts at: 593 1175 1720 2215 3112 4048 593
Size: 582 545 495 897 936 2832
Fragments arranged by size:
2832 936 897 582 545 495
MslI CAynn'nnrTG
Cuts at: 388 1379 4276 4403 388
Size: 991 2897 127 2272
Fragments arranged by size:
2897 2272 991 127
MspA1I CmG'CkG
Cuts at: 438 3146 3398 3712 4072 5085 5330 438
Size: 2708 252 314 360 1013 245 1395
Fragments arranged by size:
2708 1395 1013 360 314 252 245
NaeI GCC'GGC
Cuts at: 4468 4468
Size: 6287
NarI GG'CG_CC
Cuts at: 3718 3966 3718
Size: 248 6039
NcoI C'CATG_G
Cuts at: 621 621
Size: 6287
NgoMIV G'CCGG_C
Cuts at: 4466 4466
Size: 6287
NsiI A_TGCA'T
Cuts at: 1080 1080
Size: 6287
NspI r_CATG'y
Cuts at: 299 398 1945 3340 3840 4747 299
Size: 99 1547 1395 500 907 1839
Fragments arranged by size:
1839 1547 1395 907 500 99
PasI CC'CwG_GG
Cuts at: 1492 1492
Size: 6287
PciI A'CATG_T
Cuts at: 295 4743 295
Size: 4448 1839
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 6287
PfoI T'CCnGG_A
Cuts at: 1673 1673
Size: 6287
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PmlI CAC'GTG
Cuts at: 2465 2465
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5426 5458 5426
Size: 32 6255
PpuMI rG'GwC_Cy
Cuts at: 680 680
Size: 6287
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2926 2958 2926
Size: 32 6255
PstI C_TGCA'G
Cuts at: 182 1425 2177 3122 3334 4019 182
Size: 1243 752 945 212 685 2450
Fragments arranged by size:
2450 1243 945 752 685 212
PvuI CG_AT'CG
Cuts at: 104 3090 104
Size: 2986 3301
PvuII CAG'CTG
Cuts at: 438 3712 4072 438
Size: 3274 360 2653
Fragments arranged by size:
3274 2653 360
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 366 2897 3301 366
Size: 2531 404 3352
Fragments arranged by size:
3352 2531 404
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 2902 4310 4520 2902
Size: 1408 210 4669
Fragments arranged by size:
4669 1408 210
SbfI CC_TGCA'GG
Cuts at: 182 3334 182
Size: 3152 3135
ScaI AGT'ACT
Cuts at: 1754 2888 3157 5804 1754
Size: 1134 269 2647 2237
Fragments arranged by size:
2647 2237 1134 269
SexAI A'CCwGG_T
Cuts at: 159 159
Size: 6287
SfcI C'TryA_G
Cuts at: 33 178 1421 2140 2173 3118 3330 4015 5008
Size: 145 1243 719 33 945 212 685 993
Cuts at: 5008 5199 33
Size: 191 1121
Fragments arranged by size:
1243 1121 993 945 719 685 212 191
145 33
SfoI GGC'GCC
Cuts at: 3719 3967 3719
Size: 248 6039
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 2661 3309 2661
Size: 648 5639
SmlI C'TyrA_G
Cuts at: 2890 3442 4357 4849 5111 2890
Size: 552 915 492 262 4066
Fragments arranged by size:
4066 915 552 492 262
SnaBI TAC'GTA
Cuts at: 3279 3279
Size: 6287
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
SspI AAT'ATT
Cuts at: 542 1789 542
Size: 1247 5040
StyI C'CwwG_G
Cuts at: 311 621 1709 3421 3775 311
Size: 310 1088 1712 354 2823
Fragments arranged by size:
2823 1712 1088 354 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 443 2634 3870 4003 4462 4671 443
Size: 2191 1236 133 459 209 2059
Fragments arranged by size:
2191 2059 1236 459 209 133
TatI w'GTAC_w
Cuts at: 1251 1752 2886 3155 3203 5802 1251
Size: 501 1134 269 48 2599 1736
Fragments arranged by size:
2599 1736 1134 501 269 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5804 5836 5804
Size: 32 6255
Tth111I GACn'n_nGTC
Cuts at: 4084 4084
Size: 6287
XbaI T'CTAG_A
Cuts at: 990 1560 3318 990
Size: 570 1758 3959
Fragments arranged by size:
3959 1758 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 318 628 1728 3105 3782 318
Size: 310 1100 1377 677 2823
Fragments arranged by size:
2823 1377 1100 677 310
XmaI C'CCGG_G
Cuts at: 2659 3307 2659
Size: 648 5639
XmnI GAAnn'nnTTC
Cuts at: 646 1789 2182 2375 3029 646
Size: 1143 393 193 654 3904
Fragments arranged by size:
3904 1143 654 393 193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PshAI PsiI PspOMI PspXI SacII SanDI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI