Restriction map for
pF1KSDA0036
(Circular) (Six-base) MAPSORT of: pF1KSDA0036.seq Check: 2058 from: 1 to: 4931
pF1KSDA0036 4931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 903 903
Size: 4931
AccI GT'mk_AC
Cuts at: 903 1969 903
Size: 1066 3865
Acc65I G'GTAC_C
Cuts at: 1947 1947
Size: 4931
AclI AA'CG_TT
Cuts at: 1676 4771 1676
Size: 3095 1836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 542 1519 1726 1816 2756 3188 3935 4203
Size: 351 977 207 90 940 432 747 268
Cuts at: 4203 191
Size: 919
Fragments arranged by size:
977 940 919 747 432 351 268 207
90
AfeI AGC'GCT
Cuts at: 1406 1406
Size: 4931
AflIII A'CryG_T
Cuts at: 496 2283 3387 4364 496
Size: 1787 1104 977 1063
Fragments arranged by size:
1787 1104 1063 977
AgeI A'CCGG_T
Cuts at: 3319 3319
Size: 4931
AleI CACnn'nnGTG
Cuts at: 1815 1815
Size: 4931
AlwNI CAG_nnn'CTG
Cuts at: 2236 3803 2236
Size: 1567 3364
ApaI G_GGCC'C
Cuts at: 1620 1620
Size: 4931
ApaLI G'TGCA_C
Cuts at: 3701 3701
Size: 4931
ApoI r'AATT_y
Cuts at: 430 465 854 931 1341 1411 1890 1935 4284
Size: 35 389 77 410 70 479 45 2349
Cuts at: 4284 4540 430
Size: 256 821
Fragments arranged by size:
2349 821 479 410 389 256 77 70
45 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 4931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 1214 1951 1214
Size: 737 4194
BamHI G'GATC_C
Cuts at: 1956 1956
Size: 4931
BanI G'GyrC_C
Cuts at: 1695 1947 2361 2609 2644 1695
Size: 252 414 248 35 3982
Fragments arranged by size:
3982 414 252 248 35
BanII G_rGCy'C
Cuts at: 1540 1571 1620 1635 1736 1945 2975 1540
Size: 31 49 15 101 209 1030 3496
Fragments arranged by size:
3496 1030 209 101 49 31 15
BbeI G_GCGC'C
Cuts at: 2365 2613 2365
Size: 248 4683
BbsI GAAGACnn'nnnn_
Cuts at: 1533 1533
Size: 4931
BciVI GTATCCnnnnn_n'
Cuts at: 2829 3590 4931 2829
Size: 761 1341 2829
Fragments arranged by size:
2829 1341 761
BclI T'GATC_A
Cuts at: 624 2451 624
Size: 1827 3104
BglI GCCn_nnn'nGGC
Cuts at: 1777 1777
Size: 4931
BglII A'GATC_T
Cuts at: 2446 4483 2446
Size: 2037 2894
BlpI GC'TnA_GC
Cuts at: 2043 2043
Size: 4931
Bme1580I G_kGCm'C
Cuts at: 1620 2556 2649 3705 1620
Size: 936 93 1056 2846
Fragments arranged by size:
2846 1056 936 93
BmrI ACTGGGnnnn_n'
Cuts at: 932 2143 2224 2559 4703 932
Size: 1211 81 335 2144 1160
Fragments arranged by size:
2144 1211 1160 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1379 3298 1379
Size: 1919 3012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1862 1156
Size: 706 4225
Bpu10I CC'TnA_GC
Cuts at: 188 515 1730 188
Size: 327 1215 3389
Fragments arranged by size:
3389 1215 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2107 2986 3478 3776 1568
Size: 539 879 492 298 2723
Fragments arranged by size:
2723 879 539 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4635 4635
Size: 4931
BsaAI yAC'GTr
Cuts at: 1923 2284 1923
Size: 361 4570
BsaBI GATnn'nnATC
Cuts at: 6 2445 4482 6
Size: 2439 2037 455
Fragments arranged by size:
2439 2037 455
BsaHI Gr'CG_yC
Cuts at: 900 2362 2610 3312 4808 900
Size: 1462 248 702 1496 1023
Fragments arranged by size:
1496 1462 1023 702 248
BsaWI w'CCGG_w
Cuts at: 2120 2641 3319 3593 3740 4151 2120
Size: 521 678 274 147 411 2900
Fragments arranged by size:
2900 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 578 608 578
Size: 30 4901
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1116 1116
Size: 4931
BseYI C'CCAG_C
Cuts at: 368 1634 2356 3114 3691 4187 368
Size: 1266 722 758 577 496 1112
Fragments arranged by size:
1266 1112 758 722 577 496
BsiEI CG_ry'CG
Cuts at: 104 2473 2519 3727 104
Size: 2369 46 1208 1308
Fragments arranged by size:
2369 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1571 1736 1945 2723 2913 3705 1571
Size: 165 209 778 190 792 2797
Fragments arranged by size:
2797 792 778 209 190 165
Bsp1286I G_dGCh'C
Cuts at: 1540 1571 1620 1635 1736 1945 2556 2649 2723
Size: 31 49 15 101 209 611 93 74
Cuts at: 2723 2913 2975 3705 1540
Size: 190 62 730 2766
Fragments arranged by size:
2766 730 611 209 190 101 93 74
62 49 31 15
BspEI T'CCGG_A
Cuts at: 4151 4151
Size: 4931
BspHI T'CATG_A
Cuts at: 677 4107 677
Size: 3430 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 889 1981 2195 2497 2878 889
Size: 1092 214 302 381 2942
Fragments arranged by size:
2942 1092 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3223 3277 4761 4926 50
Size: 3173 54 1484 165 55
Fragments arranged by size:
3173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 390 2843 390
Size: 2453 2478
BsrFI r'CCGG_y
Cuts at: 2929 3110 3319 2929
Size: 181 209 4541
Fragments arranged by size:
4541 209 181
BssSI C'ACGA_G
Cuts at: 3202 3560 3202
Size: 358 4573
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 2308
Size: 4931
BstBI TT'CG_AA
Cuts at: 110 3292 110
Size: 3182 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1758 1758
Size: 4931
BstYI r'GATC_y
Cuts at: 1 152 667 1111 1747 1956 2423 2446 2781
Size: 151 515 444 636 209 467 23 335
Cuts at: 2781 3027 4039 4125 4137 4483 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 636 515 467 449 444 346 335
246 209 151 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2881 3062 3225 2881
Size: 181 163 4587
Fragments arranged by size:
4587 181 163
BtsI GCAGTG_nn'
Cuts at: 2174 2174
Size: 4931
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4931
DrdI GACnn_nn'nnGTC
Cuts at: 899 2637 3495 899
Size: 1738 858 2335
Fragments arranged by size:
2335 1738 858
EaeI y'GGCC_r
Cuts at: 2516 2690 3081 3108 4453 2516
Size: 174 391 27 1345 2994
Fragments arranged by size:
2994 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2516 2516
Size: 4931
EarI CTCTTCn'nnn_
Cuts at: 577 989 1154 1280 1619 1741 2954 3164 577
Size: 412 165 126 339 122 1213 210 2344
Fragments arranged by size:
2344 1213 412 339 210 165 126 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3449 3595 4741 3139
Size: 310 146 1146 3329
Fragments arranged by size:
3329 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1569 1734 1943 1569
Size: 165 209 4557
Fragments arranged by size:
4557 209 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 542 1156 1519 1726 1816 1862 2756 3188
Size: 351 614 363 207 90 46 894 432
Cuts at: 3188 3935 4203 191
Size: 747 268 919
Fragments arranged by size:
919 894 747 614 432 363 351 268
207 90 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1457 1457
Size: 4931
EcoO109I rG'GnC_Cy
Cuts at: 1164 1616 1617 2070 1164
Size: 452 1 453 4025
Fragments arranged by size:
4025 453 452 1
EcoRI G'AATT_C
Cuts at: 931 1935 931
Size: 1004 3927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4931
FspI TGC'GCA
Cuts at: 2712 2712
Size: 4931
HaeII r_GCGC'y
Cuts at: 1408 2365 2613 3635 4614 1408
Size: 957 248 1022 979 1725
Fragments arranged by size:
1725 1022 979 957 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 552 584 659 691 2773 2803 2805 2835 552
Size: 32 75 32 2082 30 2 30 2648
Fragments arranged by size:
2648 2082 75 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1970 1970
Size: 4931
HindIII A'AGCT_T
Cuts at: 775 1072 1825 775
Size: 297 753 3881
Fragments arranged by size:
3881 753 297
KasI G'GCGC_C
Cuts at: 2361 2609 2361
Size: 248 4683
KpnI G_GTAC'C
Cuts at: 1951 1951
Size: 4931
MfeI C'AATT_G
Cuts at: 1177 1177
Size: 4931
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 334 3602 3786 334
Size: 3268 184 1479
Fragments arranged by size:
3268 1479 184
MscI TGG'CCA
Cuts at: 2692 2692
Size: 4931
MslI CAynn'nnrTG
Cuts at: 395 682 1815 2920 3047 395
Size: 287 1133 1105 127 2279
Fragments arranged by size:
2279 1133 1105 287 127
MspA1I CmG'CkG
Cuts at: 457 1227 1384 2042 2356 2716 3729 3974 457
Size: 770 157 658 314 360 1013 245 1414
Fragments arranged by size:
1414 1013 770 658 360 314 245 157
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 4931
NarI GG'CG_CC
Cuts at: 2362 2610 2362
Size: 248 4683
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 4931
NspI r_CATG'y
Cuts at: 500 1022 1984 2484 3391 500
Size: 522 962 500 907 2040
Fragments arranged by size:
2040 962 907 522 500
PciI A'CATG_T
Cuts at: 496 3387 496
Size: 2891 2040
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1718 1750 4070 4102 1718
Size: 32 2320 32 2547
Fragments arranged by size:
2547 2320 32 32
PpuMI rG'GwC_Cy
Cuts at: 1164 1164
Size: 4931
PsiI TTA'TAA
Cuts at: 802 802
Size: 4931
PspOMI G'GGCC_C
Cuts at: 1616 1616
Size: 4931
PstI C_TGCA'G
Cuts at: 462 675 1978 2663 462
Size: 213 1303 685 2730
Fragments arranged by size:
2730 1303 685 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 457 1227 1384 2356 2716 457
Size: 770 157 972 360 2672
Fragments arranged by size:
2672 972 770 360 157
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 1571 1736 1945 1571
Size: 165 209 4557
Fragments arranged by size:
4557 209 165
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SapI GCTCTTCn'nnn_
Cuts at: 989 1619 1741 2954 3164 989
Size: 630 122 1213 210 2756
Fragments arranged by size:
2756 1213 630 210 122
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 810 4448 810
Size: 3638 1293
SfcI C'TryA_G
Cuts at: 33 161 458 671 688 1974 2659 3652 3843
Size: 128 297 213 17 1286 685 993 191
Cuts at: 3843 33
Size: 1121
Fragments arranged by size:
1286 1121 993 685 297 213 191 128
17
SfiI GGCCn_nnn'nGGCC
Cuts at: 1777 1777
Size: 4931
SfoI GGC'GCC
Cuts at: 2363 2611 2363
Size: 248 4683
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 1953 1953
Size: 4931
SmlI C'TyrA_G
Cuts at: 1583 2086 3001 3493 3755 1583
Size: 503 915 492 262 2759
Fragments arranged by size:
2759 915 503 492 262
SnaBI TAC'GTA
Cuts at: 1923 1923
Size: 4931
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4931
SphI G_CATG'C
Cuts at: 1022 1984 2484 1022
Size: 962 500 3469
Fragments arranged by size:
3469 962 500
SspI AAT'ATT
Cuts at: 307 307
Size: 4931
StuI AGG'CCT
Cuts at: 1755 1755
Size: 4931
StyI C'CwwG_G
Cuts at: 148 1453 1766 2065 2419 148
Size: 1305 313 299 354 2660
Fragments arranged by size:
2660 1305 354 313 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2514 2647 3106 3315 2514
Size: 133 459 209 4130
Fragments arranged by size:
4130 459 209 133
TatI w'GTAC_w
Cuts at: 612 808 4446 612
Size: 196 3638 1097
Fragments arranged by size:
3638 1097 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1289 1321 4448 4480 1289
Size: 32 3127 32 1740
Fragments arranged by size:
3127 1740 32 32
Tth111I GACn'n_nGTC
Cuts at: 655 1519 2728 655
Size: 864 1209 2858
Fragments arranged by size:
2858 1209 864
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 4931
XmaI C'CCGG_G
Cuts at: 1951 1951
Size: 4931
XmnI GAAnn'nnTTC
Cuts at: 446 467 602 1244 446
Size: 21 135 642 4133
Fragments arranged by size:
4133 642 135 21
ZraI GAC'GTC
Cuts at: 901 901
Size: 4931
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspXI PsrI SacII SanDI SexAI SgrAI
SrfI SwaI XhoI