Restriction map for
pF1KSDA0039
(Circular) (Six-base) MAPSORT of: pF1KSDA0039.seq Check: 4167 from: 1 to: 4535
pF1KSDA0039 4535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 672 672
Size: 4535
AccI GT'mk_AC
Cuts at: 1337 1573 1337
Size: 236 4299
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3539 3807 2360
Size: 432 747 268 3088
Fragments arranged by size:
3088 747 432 268
AflIII A'CryG_T
Cuts at: 608 1278 1887 2991 3968 608
Size: 670 609 1104 977 1175
Fragments arranged by size:
1175 1104 977 670 609
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 246 931 994 1840 3407 246
Size: 685 63 846 1567 1374
Fragments arranged by size:
1567 1374 846 685 63
ApaI G_GGCC'C
Cuts at: 1453 1453
Size: 4535
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 279 517 862 1539 3888 4144 279
Size: 238 345 677 2349 256 670
Fragments arranged by size:
2349 677 670 345 256 238
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 807 1551 1965 2213 2248 807
Size: 744 414 248 35 3094
Fragments arranged by size:
3094 744 414 248 35
BanII G_rGCy'C
Cuts at: 291 558 1249 1453 1549 2579 291
Size: 267 691 204 96 1030 2247
Fragments arranged by size:
2247 1030 691 267 204 96
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 569 1390 569
Size: 821 3714
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BfrBI ATG'CAT
Cuts at: 787 787
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 202 1647 202
Size: 1445 3090
Bme1580I G_kGCm'C
Cuts at: 1453 2160 2253 3309 1453
Size: 707 93 1056 2679
Fragments arranged by size:
2679 1056 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BmtI G_CTAG'C
Cuts at: 403 403
Size: 4535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1233 1711 2590 3082 3380 1233
Size: 478 879 492 298 2388
Fragments arranged by size:
2388 879 492 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 4239 4239
Size: 4535
BsaAI yAC'GTr
Cuts at: 1527 1888 1527
Size: 361 4174
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3197 3344 3755 1724
Size: 521 678 274 147 411 2504
Fragments arranged by size:
2504 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1101 1172 1175 1384 1101
Size: 71 3 209 4252
Fragments arranged by size:
4252 209 71 3
BseYI C'CCAG_C
Cuts at: 683 1960 2718 3295 3791 683
Size: 1277 758 577 496 1427
Fragments arranged by size:
1427 1277 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1338 557
Size: 781 3754
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 558 1249 1549 2327 2517 3309 558
Size: 691 300 778 190 792 1784
Fragments arranged by size:
1784 792 778 691 300 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1196 1196
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 291 558 1249 1453 1549 2160 2253 2327 2517
Size: 267 691 204 96 611 93 74 190
Cuts at: 2517 2579 3309 291
Size: 62 730 1517
Fragments arranged by size:
1517 730 691 611 267 204 190 96
93 74 62
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 666 672 1585 1799 2101 2482 666
Size: 6 913 214 302 381 2719
Fragments arranged by size:
2719 913 381 302 214 6
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2447 2447
Size: 4535
BsrFI r'CCGG_y
Cuts at: 2533 2714 2923 2533
Size: 181 209 4145
Fragments arranged by size:
4145 209 181
BsrGI T'GTAC_A
Cuts at: 425 425
Size: 4535
BssSI C'ACGA_G
Cuts at: 2806 3164 2806
Size: 358 4177
BstAPI GCAn_nnn'nTGC
Cuts at: 958 1912 958
Size: 954 3581
BstBI TT'CG_AA
Cuts at: 110 2896 110
Size: 2786 1749
BstYI r'GATC_y
Cuts at: 1 1560 2027 2050 2385 2631 3643 3729 3741
Size: 1559 467 23 335 246 1012 86 12
Cuts at: 3741 4087 1
Size: 346 449
Fragments arranged by size:
1559 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 392 899 1125 1778 392
Size: 507 226 653 3149
Fragments arranged by size:
3149 653 507 226
DraI TTT'AAA
Cuts at: 867 1534 867
Size: 667 3868
DrdI GACnn_nn'nnGTC
Cuts at: 163 2241 3099 163
Size: 2078 858 1599
Fragments arranged by size:
2078 1599 858
EaeI y'GGCC_r
Cuts at: 2120 2294 2685 2712 4057 2120
Size: 174 391 27 1345 2598
Fragments arranged by size:
2598 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 576 1361 2558 2768 576
Size: 785 1197 210 2343
Fragments arranged by size:
2343 1197 785 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 2743 3053 3199 4345 145
Size: 2598 310 146 1146 335
Fragments arranged by size:
2598 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 556 1247 1547 556
Size: 691 300 3544
Fragments arranged by size:
3544 691 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3539 3807 2360
Size: 432 747 268 3088
Fragments arranged by size:
3088 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1449 1450 1674 1449
Size: 1 224 4310
Fragments arranged by size:
4310 224 1
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1390 2554 1390
Size: 1164 3371
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1969 2217 3239 4218 1969
Size: 248 1022 979 2286
Fragments arranged by size:
2286 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1156 1188 1333 1365 1455 1487 2377 2407 2409
Size: 32 145 32 90 32 890 30 2
Cuts at: 2409 2439 1156
Size: 30 3252
Fragments arranged by size:
3252 890 145 90 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 228 1574 228
Size: 1346 3189
HpaI GTT'AAC
Cuts at: 228 228
Size: 4535
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 1278 3968 1278
Size: 2690 1845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3390 3206
Size: 184 4351
MscI TGG'CCA
Cuts at: 2296 2296
Size: 4535
MslI CAynn'nnrTG
Cuts at: 195 421 1417 2524 2651 195
Size: 226 996 1107 127 2079
Fragments arranged by size:
2079 1107 996 226 127
MspA1I CmG'CkG
Cuts at: 541 1646 1960 2320 3333 3578 541
Size: 1105 314 360 1013 245 1498
Fragments arranged by size:
1498 1105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 125 125
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NheI G'CTAG_C
Cuts at: 399 399
Size: 4535
NsiI A_TGCA'T
Cuts at: 789 789
Size: 4535
NspI r_CATG'y
Cuts at: 301 612 1588 2088 2995 301
Size: 311 976 500 907 1841
Fragments arranged by size:
1841 976 907 500 311
PciI A'CATG_T
Cuts at: 608 2991 608
Size: 2383 2152
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PshAI GACnn'nnGTC
Cuts at: 1494 1494
Size: 4535
PspOMI G'GGCC_C
Cuts at: 1449 1449
Size: 4535
PstI C_TGCA'G
Cuts at: 514 795 963 1010 1582 2267 514
Size: 281 168 47 572 685 2782
Fragments arranged by size:
2782 685 572 281 168 47
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 541 1960 2320 541
Size: 1419 360 2756
Fragments arranged by size:
2756 1419 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 558 1249 1549 558
Size: 691 300 3544
Fragments arranged by size:
3544 691 300
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 963 1582 963
Size: 619 3916
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SfcI C'TryA_G
Cuts at: 33 510 791 923 959 1006 1578 2263 3256
Size: 477 281 132 36 47 572 685 993
Cuts at: 3256 3447 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 572 477 281 191 132
47 36
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1248 1690 2605 3097 3359 1248
Size: 442 915 492 262 2424
Fragments arranged by size:
2424 915 492 442 262
SnaBI TAC'GTA
Cuts at: 1527 1527
Size: 4535
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StuI AGG'CCT
Cuts at: 965 965
Size: 4535
StyI C'CwwG_G
Cuts at: 125 1669 2023 125
Size: 1544 354 2637
Fragments arranged by size:
2637 1544 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2118 2251 2710 2919 2118
Size: 133 459 209 3734
Fragments arranged by size:
3734 459 209 133
TatI w'GTAC_w
Cuts at: 425 1395 4050 425
Size: 970 2655 910
Fragments arranged by size:
2655 970 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 2030 699
Size: 1331 3204
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
XmnI GAAnn'nnTTC
Cuts at: 1133 1133
Size: 4535
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BpmI Bpu10I
BsaXI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI