Restriction map for
pF1KSDA0043
(Circular) (Six-base) MAPSORT of: pF1KSDA0043.seq Check: 170 from: 1 to: 5315
pF1KSDA0043 5315 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1067 1067
Size: 5315
AatII G_ACGT'C
Cuts at: 886 886
Size: 5315
AccI GT'mk_AC
Cuts at: 2353 2353
Size: 5315
Acc65I G'GTAC_C
Cuts at: 2331 2331
Size: 5315
AclI AA'CG_TT
Cuts at: 5155 5155
Size: 5315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 1795 3140 3572 4319 4587 1555
Size: 240 1345 432 747 268 2283
Fragments arranged by size:
2283 1345 747 432 268 240
AflIII A'CryG_T
Cuts at: 1343 2667 3771 4748 1343
Size: 1324 1104 977 1910
Fragments arranged by size:
1910 1324 1104 977
AgeI A'CCGG_T
Cuts at: 3703 3703
Size: 5315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1814 1814
Size: 5315
AlwNI CAG_nnn'CTG
Cuts at: 2620 4187 2620
Size: 1567 3748
ApaI G_GGCC'C
Cuts at: 178 2221 178
Size: 2043 3272
ApaLI G'TGCA_C
Cuts at: 1543 1912 4085 1543
Size: 369 2173 2773
Fragments arranged by size:
2773 2173 369
ApoI r'AATT_y
Cuts at: 518 2319 4668 4924 518
Size: 1801 2349 256 909
Fragments arranged by size:
2349 1801 909 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5315
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5315
AvaI C'yCGr_G
Cuts at: 171 1888 1928 2335 171
Size: 1717 40 407 3151
Fragments arranged by size:
3151 1717 407 40
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 710 743 710
Size: 33 5282
BamHI G'GATC_C
Cuts at: 158 2340 158
Size: 2182 3133
BanI G'GyrC_C
Cuts at: 1017 1389 2331 2745 2993 3028 1017
Size: 372 942 414 248 35 3304
Fragments arranged by size:
3304 942 414 372 248 35
BanII G_rGCy'C
Cuts at: 178 615 1382 1465 1937 2221 2329 3359 178
Size: 437 767 83 472 284 108 1030 2134
Fragments arranged by size:
2134 1030 767 472 437 284 108 83
BbeI G_GCGC'C
Cuts at: 2749 2997 2749
Size: 248 5067
BbsI GAAGACnn'nnnn_
Cuts at: 274 274
Size: 5315
BbvCI CC'TCA_GC
Cuts at: 1198 1198
Size: 5315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2072 2106 2072
Size: 34 5281
BciVI GTATCCnnnnn_n'
Cuts at: 3213 3974 5315 3213
Size: 761 1341 3213
Fragments arranged by size:
3213 1341 761
BclI T'GATC_A
Cuts at: 2835 2835
Size: 5315
BglI GCCn_nnn'nGGC
Cuts at: 1411 1693 1711 1741 1411
Size: 282 18 30 4985
Fragments arranged by size:
4985 282 30 18
BglII A'GATC_T
Cuts at: 2830 4867 2830
Size: 2037 3278
BlpI GC'TnA_GC
Cuts at: 1715 1864 2161 2202 2230 2427 1715
Size: 149 297 41 28 197 4603
Fragments arranged by size:
4603 297 197 149 41 28
Bme1580I G_kGCm'C
Cuts at: 178 686 1022 1547 1916 2221 2940 3033 4089
Size: 508 336 525 369 305 719 93 1056
Cuts at: 4089 178
Size: 1404
Fragments arranged by size:
1404 1056 719 525 508 369 336 305
93
BmgBI CAC'GTC
Cuts at: 752 1344 752
Size: 592 4723
BmrI ACTGGGnnnn_n'
Cuts at: 754 1570 2527 2608 2943 5087 754
Size: 816 957 81 335 2144 982
Fragments arranged by size:
2144 982 957 816 335 81
BmtI G_CTAG'C
Cuts at: 2107 2107
Size: 5315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1479 3682 1479
Size: 2203 3112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1174 1321 2032 2253 1024
Size: 150 147 711 221 4086
Fragments arranged by size:
4086 711 221 150 147
Bpu10I CC'TnA_GC
Cuts at: 1198 1942 2108 1198
Size: 744 166 4405
Fragments arranged by size:
4405 744 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2491 3370 3862 4160 2491
Size: 879 492 298 3646
Fragments arranged by size:
3646 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 207 5019 207
Size: 4812 503
BsaAI yAC'GTr
Cuts at: 2307 2668 2307
Size: 361 4954
BsaBI GATnn'nnATC
Cuts at: 6 2829 4866 6
Size: 2823 2037 455
Fragments arranged by size:
2823 2037 455
BsaHI Gr'CG_yC
Cuts at: 703 883 2746 2994 3696 5192 703
Size: 180 1863 248 702 1496 826
Fragments arranged by size:
1863 1496 826 702 248 180
BsaWI w'CCGG_w
Cuts at: 2504 3025 3703 3977 4124 4535 2504
Size: 521 678 274 147 411 3284
Fragments arranged by size:
3284 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 858 888 858
Size: 30 5285
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 776 1471 1507 1510 1837 1840 2113 2235 776
Size: 695 36 3 327 3 273 122 3856
Fragments arranged by size:
3856 695 327 273 122 36 3 3
BseYI C'CCAG_C
Cuts at: 211 660 768 1022 1899 2740 3498 4075 4571
Size: 449 108 254 877 841 758 577 496
Cuts at: 4571 211
Size: 955
Fragments arranged by size:
955 877 841 758 577 496 449 254
108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1144
Size: 5315
BsiEI CG_ry'CG
Cuts at: 104 225 644 1763 2857 2903 4111 104
Size: 121 419 1119 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1119 1094 419 121 46
BsiHKAI G_wGCw'C
Cuts at: 1058 1465 1547 1916 2329 3107 3297 4089 1058
Size: 407 82 369 413 778 190 792 2284
Fragments arranged by size:
2284 792 778 413 407 369 190 82
BsmBI CGTCTCn'nnnn_
Cuts at: 699 699
Size: 5315
Bsp1286I G_dGCh'C
Cuts at: 178 615 686 1022 1058 1382 1465 1547 1916
Size: 437 71 336 36 324 83 82 369
Cuts at: 1916 1937 2221 2329 2940 3033 3107 3297 3359
Size: 21 284 108 611 93 74 190 62
Cuts at: 3359 4089 178
Size: 730 1404
Fragments arranged by size:
1404 730 611 437 369 336 324 284
190 108 93 83 82 74 71 62
36 21
BspEI T'CCGG_A
Cuts at: 4535 4535
Size: 5315
BspHI T'CATG_A
Cuts at: 4491 4491
Size: 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 341 613 1067 2365 2579 2881 3262 341
Size: 272 454 1298 214 302 381 2394
Fragments arranged by size:
2394 1298 454 381 302 272 214
BsrBI CCG'CTC
Cuts at: 50 1500 3607 3661 5145 5310 50
Size: 1450 2107 54 1484 165 55
Fragments arranged by size:
2107 1484 1450 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3227 3227
Size: 5315
BsrFI r'CCGG_y
Cuts at: 600 1392 1762 1880 3313 3494 3703 600
Size: 792 370 118 1433 181 209 2212
Fragments arranged by size:
2212 1433 792 370 209 181 118
BssSI C'ACGA_G
Cuts at: 1309 1546 3586 3944 1309
Size: 237 2040 358 2680
Fragments arranged by size:
2680 2040 358 237
BstAPI GCAn_nnn'nTGC
Cuts at: 249 2692 249
Size: 2443 2872
BstBI TT'CG_AA
Cuts at: 110 3676 110
Size: 3566 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 951 1512 951
Size: 561 4754
BstYI r'GATC_y
Cuts at: 1 158 2340 2807 2830 3165 3411 4423 4509
Size: 157 2182 467 23 335 246 1012 86
Cuts at: 4509 4521 4867 1
Size: 12 346 449
Fragments arranged by size:
2182 1012 467 449 346 335 246 157
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1081 2211 1081
Size: 1130 4185
BtgI C'CryG_G
Cuts at: 152 311 809 1322 1382 1409 1418 1757 152
Size: 159 498 513 60 27 9 339 3710
Fragments arranged by size:
3710 513 498 339 159 60 27 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 696 3265 3446 3609 696
Size: 2569 181 163 2402
Fragments arranged by size:
2569 2402 181 163
BtsI GCAGTG_nn'
Cuts at: 737 893 1458 2298 2558 737
Size: 156 565 840 260 3494
Fragments arranged by size:
3494 840 565 260 156
DraI TTT'AAA
Cuts at: 2314 2314
Size: 5315
DraIII CAC_nnn'GTG
Cuts at: 1662 1662
Size: 5315
DrdI GACnn_nn'nnGTC
Cuts at: 3021 3879 3021
Size: 858 4457
EaeI y'GGCC_r
Cuts at: 222 641 887 972 1224 1319 1556 1760 2900
Size: 419 246 85 252 95 237 204 1140
Cuts at: 2900 3074 3465 3492 4837 222
Size: 174 391 27 1345 700
Fragments arranged by size:
1345 1140 700 419 391 252 246 237
204 174 95 85 27
EagI C'GGCC_G
Cuts at: 222 641 1760 2900 222
Size: 419 1119 1140 2637
Fragments arranged by size:
2637 1140 1119 419
EarI CTCTTCn'nnn_
Cuts at: 382 1470 1670 1814 3338 3548 382
Size: 1088 200 144 1524 210 2149
Fragments arranged by size:
2149 1524 1088 210 200 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1531 1688 3523 3833 3979 5125 1531
Size: 157 1835 310 146 1146 1721
Fragments arranged by size:
1835 1721 1146 310 157 146
EcoICRI GAG'CTC
Cuts at: 1463 2327 1463
Size: 864 4451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1174 1321 1555 1795 2032 2253 3140 3572
Size: 150 147 234 240 237 221 887 432
Cuts at: 3572 4319 4587 1024
Size: 747 268 1752
Fragments arranged by size:
1752 887 747 432 268 240 237 234
221 150 147
EcoNI CCTnn'n_nnAGG
Cuts at: 1567 1941 1567
Size: 374 4941
EcoO109I rG'GnC_Cy
Cuts at: 175 999 1149 1572 1830 2217 2454 175
Size: 824 150 423 258 387 237 3036
Fragments arranged by size:
3036 824 423 387 258 237 150
EcoRI G'AATT_C
Cuts at: 2319 2319
Size: 5315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 5315
FspI TGC'GCA
Cuts at: 3096 3096
Size: 5315
HaeII r_GCGC'y
Cuts at: 1399 1436 1860 2749 2997 4019 4998 1399
Size: 37 424 889 248 1022 979 1716
Fragments arranged by size:
1716 1022 979 889 424 248 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1078 1110 3157 3187 3189 3219 1078
Size: 32 2047 30 2 30 3174
Fragments arranged by size:
3174 2047 32 30 30 2
HincII GTy'rAC
Cuts at: 2354 2354
Size: 5315
HindIII A'AGCT_T
Cuts at: 506 506
Size: 5315
KasI G'GCGC_C
Cuts at: 2745 2993 2745
Size: 248 5067
KpnI G_GTAC'C
Cuts at: 2335 2335
Size: 5315
MluI A'CGCG_T
Cuts at: 4748 4748
Size: 5315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 574 1031 1976 3986 4170 230
Size: 344 457 945 2010 184 1375
Fragments arranged by size:
2010 1375 945 457 344 184
MscI TGG'CCA
Cuts at: 1321 3076 1321
Size: 1755 3560
MslI CAynn'nnrTG
Cuts at: 3304 3431 3304
Size: 127 5188
MspA1I CmG'CkG
Cuts at: 154 240 772 1411 1533 1554 1773 2153 2160
Size: 86 532 639 122 21 219 380 7
Cuts at: 2160 2273 2426 2740 3100 4113 4358 154
Size: 113 153 314 360 1013 245 1111
Fragments arranged by size:
1111 1013 639 532 380 360 314 245
219 153 122 113 86 21 7
NaeI GCC'GGC
Cuts at: 602 1764 3496 602
Size: 1162 1732 2421
Fragments arranged by size:
2421 1732 1162
NarI GG'CG_CC
Cuts at: 2746 2994 2746
Size: 248 5067
NcoI C'CATG_G
Cuts at: 1322 1418 1322
Size: 96 5219
NgoMIV G'CCGG_C
Cuts at: 600 1762 3494 600
Size: 1162 1732 2421
Fragments arranged by size:
2421 1732 1162
NheI G'CTAG_C
Cuts at: 2103 2103
Size: 5315
NspI r_CATG'y
Cuts at: 252 2368 2868 3775 252
Size: 2116 500 907 1792
Fragments arranged by size:
2116 1792 907 500
PciI A'CATG_T
Cuts at: 3771 3771
Size: 5315
PflMI CCAn_nnn'nTGG
Cuts at: 312 312
Size: 5315
PfoI T'CCnGG_A
Cuts at: 1337 1337
Size: 5315
PmeI GTTT'AAAC
Cuts at: 2314 2314
Size: 5315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 4454
Size: 32 5283
PpuMI rG'GwC_Cy
Cuts at: 999 999
Size: 5315
PshAI GACnn'nnGTC
Cuts at: 706 706
Size: 5315
PsiI TTA'TAA
Cuts at: 470 470
Size: 5315
PspOMI G'GGCC_C
Cuts at: 174 2217 174
Size: 2043 3272
PstI C_TGCA'G
Cuts at: 245 1526 2144 2362 3047 245
Size: 1281 618 218 685 2513
Fragments arranged by size:
2513 1281 685 618 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5315
PvuII CAG'CTG
Cuts at: 240 772 1533 1554 1773 2160 2740 3100 240
Size: 532 761 21 219 387 580 360 2455
Fragments arranged by size:
2455 761 580 532 387 360 219 21
RsrII CG'GwC_CG
Cuts at: 3510 3510
Size: 5315
SacI G_AGCT'C
Cuts at: 1465 2329 1465
Size: 864 4451
SacII CC_GC'GG
Cuts at: 155 1412 155
Size: 1257 4058
SalI G'TCGA_C
Cuts at: 2352 2352
Size: 5315
SapI GCTCTTCn'nnn_
Cuts at: 1470 3338 3548 1470
Size: 1868 210 3237
Fragments arranged by size:
3237 1868 210
SbfI CC_TGCA'GG
Cuts at: 2362 2362
Size: 5315
ScaI AGT'ACT
Cuts at: 4832 4832
Size: 5315
SfcI C'TryA_G
Cuts at: 33 241 1522 2140 2358 3043 4036 4227 33
Size: 208 1281 618 218 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 618 218 208 191
SfoI GGC'GCC
Cuts at: 2747 2995 2747
Size: 248 5067
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5315
SgrAI Cr'CCGG_yG
Cuts at: 1392 1392
Size: 5315
SmaI CCC'GGG
Cuts at: 173 1890 2337 173
Size: 1717 447 3151
Fragments arranged by size:
3151 1717 447
SmlI C'TyrA_G
Cuts at: 2470 3385 3877 4139 2470
Size: 915 492 262 3646
Fragments arranged by size:
3646 915 492 262
SnaBI TAC'GTA
Cuts at: 2307 2307
Size: 5315
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5315
SphI G_CATG'C
Cuts at: 2368 2868 2368
Size: 500 4815
StyI C'CwwG_G
Cuts at: 1322 1418 1688 2449 2803 1322
Size: 96 270 761 354 3834
Fragments arranged by size:
3834 761 354 270 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 744 813 2898 3031 3490 3699 744
Size: 69 2085 133 459 209 2360
Fragments arranged by size:
2360 2085 459 209 133 69
TatI w'GTAC_w
Cuts at: 245 4830 245
Size: 4585 730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 788 820 858 890 4832 4864 788
Size: 32 38 32 3942 32 1239
Fragments arranged by size:
3942 1239 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3112 3112
Size: 5315
XbaI T'CTAG_A
Cuts at: 2346 2346
Size: 5315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 807 999 2810 807
Size: 192 1811 3312
Fragments arranged by size:
3312 1811 192
XmaI C'CCGG_G
Cuts at: 171 1888 2335 171
Size: 1717 447 3151
Fragments arranged by size:
3151 1717 447
XmnI GAAnn'nnTTC
Cuts at: 2056 2056
Size: 5315
ZraI GAC'GTC
Cuts at: 884 884
Size: 5315
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BfrBI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PmlI PspXI PsrI SanDI SexAI
SfiI SrfI SspI StuI SwaI XhoI