Restriction map for
pF1KSDA0046
(Circular) (Six-base) MAPSORT of: pF1KSDA0046.seq Check: 3468 from: 1 to: 5744
pF1KSDA0046 5744 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 152 2636 152
Size: 2484 3260
AccI GT'mk_AC
Cuts at: 1961 2782 1961
Size: 821 4923
Acc65I G'GTAC_C
Cuts at: 904 2474 2760 904
Size: 1570 286 3888
Fragments arranged by size:
3888 1570 286
AclI AA'CG_TT
Cuts at: 5584 5584
Size: 5744
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 4001 4748 5016 3569
Size: 432 747 268 4297
Fragments arranged by size:
4297 747 432 268
AflIII A'CryG_T
Cuts at: 2189 3096 4200 5177 2189
Size: 907 1104 977 2756
Fragments arranged by size:
2756 1104 977 907
AgeI A'CCGG_T
Cuts at: 4132 4132
Size: 5744
AleI CACnn'nnGTG
Cuts at: 1285 1285
Size: 5744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1484 1484
Size: 5744
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2314 2346 2314
Size: 32 5712
AlwNI CAG_nnn'CTG
Cuts at: 1986 2253 3049 4616 1986
Size: 267 796 1567 3114
Fragments arranged by size:
3114 1567 796 267
ApaLI G'TGCA_C
Cuts at: 1222 4514 1222
Size: 3292 2452
ApoI r'AATT_y
Cuts at: 1389 2692 2748 5097 5353 1389
Size: 1303 56 2349 256 1780
Fragments arranged by size:
2349 1780 1303 256 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 5744
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5744
AvaI C'yCGr_G
Cuts at: 202 604 2123 2764 202
Size: 402 1519 641 3182
Fragments arranged by size:
3182 1519 641 402
BamHI G'GATC_C
Cuts at: 2769 2769
Size: 5744
BanI G'GyrC_C
Cuts at: 636 891 904 2474 2760 3174 3422 3457 636
Size: 255 13 1570 286 414 248 35 2923
Fragments arranged by size:
2923 1570 414 286 255 248 35 13
BanII G_rGCy'C
Cuts at: 710 953 1790 1946 2758 3788 710
Size: 243 837 156 812 1030 2666
Fragments arranged by size:
2666 1030 837 812 243 156
BbeI G_GCGC'C
Cuts at: 895 3178 3426 895
Size: 2283 248 3213
Fragments arranged by size:
3213 2283 248
BbsI GAAGACnn'nnnn_
Cuts at: 540 2315 2586 540
Size: 1775 271 3698
Fragments arranged by size:
3698 1775 271
BbvCI CC'TCA_GC
Cuts at: 2710 2710
Size: 5744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2601 2635 2601
Size: 34 5710
BciVI GTATCCnnnnn_n'
Cuts at: 593 3642 4403 5744 593
Size: 3049 761 1341 593
Fragments arranged by size:
3049 1341 761 593
BclI T'GATC_A
Cuts at: 3264 3264
Size: 5744
BglI GCCn_nnn'nGGC
Cuts at: 494 1874 2455 494
Size: 1380 581 3783
Fragments arranged by size:
3783 1380 581
BglII A'GATC_T
Cuts at: 3259 5296 3259
Size: 2037 3707
BlpI GC'TnA_GC
Cuts at: 2856 2856
Size: 5744
Bme1580I G_kGCm'C
Cuts at: 1226 3369 3462 4518 1226
Size: 2143 93 1056 2452
Fragments arranged by size:
2452 2143 1056 93
BmgBI CAC'GTC
Cuts at: 2583 2583
Size: 5744
BmrI ACTGGGnnnn_n'
Cuts at: 2956 3037 3372 5516 2956
Size: 81 335 2144 3184
Fragments arranged by size:
3184 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 781 2285 4111 781
Size: 1504 1826 2414
Fragments arranged by size:
2414 1826 1504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 890 1354 1462 722
Size: 168 464 108 5004
Fragments arranged by size:
5004 464 168 108
Bpu10I CC'TnA_GC
Cuts at: 1235 2710 1235
Size: 1475 4269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 2920 3799 4291 4589 433
Size: 2487 879 492 298 1588
Fragments arranged by size:
2487 1588 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 149 184 316 415 5448 149
Size: 35 132 99 5033 445
Fragments arranged by size:
5033 445 132 99 35
BsaAI yAC'GTr
Cuts at: 956 1863 2736 3097 956
Size: 907 873 361 3603
Fragments arranged by size:
3603 907 873 361
BsaBI GATnn'nnATC
Cuts at: 6 284 3258 5295 6
Size: 278 2974 2037 455
Fragments arranged by size:
2974 2037 455 278
BsaHI Gr'CG_yC
Cuts at: 892 3175 3423 4125 5621 892
Size: 2283 248 702 1496 1015
Fragments arranged by size:
2283 1496 1015 702 248
BsaWI w'CCGG_w
Cuts at: 656 2933 3454 4132 4406 4553 4964 656
Size: 2277 521 678 274 147 411 1436
Fragments arranged by size:
2277 1436 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 916 946 2314 2344 2642 2672 916
Size: 30 1368 30 298 30 3988
Fragments arranged by size:
3988 1368 298 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 250 253 493 937 2230 2338 2408 220
Size: 30 3 240 444 1293 108 70 3556
Fragments arranged by size:
3556 1293 444 240 108 70 30 3
BseYI C'CCAG_C
Cuts at: 1031 3169 3927 4504 5000 1031
Size: 2138 758 577 496 1775
Fragments arranged by size:
2138 1775 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 378 504 1721 156
Size: 222 126 1217 4179
Fragments arranged by size:
4179 1217 222 126
BsiEI CG_ry'CG
Cuts at: 104 2368 3286 3332 4540 104
Size: 2264 918 46 1208 1308
Fragments arranged by size:
2264 1308 1208 918 46
BsiHKAI G_wGCw'C
Cuts at: 968 1084 1226 2204 2758 3536 3726 4518 968
Size: 116 142 978 554 778 190 792 2194
Fragments arranged by size:
2194 978 792 778 554 190 142 116
BsmI GAATG_Cn'
Cuts at: 1324 1324
Size: 5744
BsmBI CGTCTCn'nnnn_
Cuts at: 658 2571 2589 658
Size: 1913 18 3813
Fragments arranged by size:
3813 1913 18
Bsp1286I G_dGCh'C
Cuts at: 710 953 968 1084 1226 1790 1946 2204 2758
Size: 243 15 116 142 564 156 258 554
Cuts at: 2758 3369 3462 3536 3726 3788 4518 710
Size: 611 93 74 190 62 730 1936
Fragments arranged by size:
1936 730 611 564 554 258 243 190
156 142 116 93 74 62 15
BspEI T'CCGG_A
Cuts at: 656 4964 656
Size: 4308 1436
BspHI T'CATG_A
Cuts at: 2063 4920 2063
Size: 2857 2887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 580 2636 2794 3008 3310 3691 152
Size: 428 2056 158 214 302 381 2205
Fragments arranged by size:
2205 2056 428 381 302 214 158
BsrBI CCG'CTC
Cuts at: 50 862 2328 4036 4090 5574 5739 50
Size: 812 1466 1708 54 1484 165 55
Fragments arranged by size:
1708 1484 1466 812 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3656 3656
Size: 5744
BsrFI r'CCGG_y
Cuts at: 579 3742 3923 4132 579
Size: 3163 181 209 2191
Fragments arranged by size:
3163 2191 209 181
BssSI C'ACGA_G
Cuts at: 4015 4373 4015
Size: 358 5386
BstAPI GCAn_nnn'nTGC
Cuts at: 167 2250 3121 167
Size: 2083 871 2790
Fragments arranged by size:
2790 2083 871
BstBI TT'CG_AA
Cuts at: 110 4105 110
Size: 3995 1749
BstEII G'GTnAC_C
Cuts at: 1275 1275
Size: 5744
BstXI CCAn_nnnn'nTGG
Cuts at: 1051 1287 1495 1051
Size: 236 208 5300
Fragments arranged by size:
5300 236 208
BstYI r'GATC_y
Cuts at: 1 797 2769 3236 3259 3594 3840 4852 4938
Size: 796 1972 467 23 335 246 1012 86
Cuts at: 4938 4950 5296 1
Size: 12 346 449
Fragments arranged by size:
1972 1012 796 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 326 1283 2006 2043 2318 2349 2470 2633
Size: 201 957 723 37 275 31 121 163
Cuts at: 2633 125
Size: 3236
Fragments arranged by size:
3236 957 723 275 201 163 121 37
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2190 3694 3875 4038 2190
Size: 1504 181 163 3896
Fragments arranged by size:
3896 1504 181 163
BtsI GCAGTG_nn'
Cuts at: 2987 2987
Size: 5744
DraI TTT'AAA
Cuts at: 2743 2743
Size: 5744
DrdI GACnn_nn'nnGTC
Cuts at: 3450 4308 3450
Size: 858 4886
EaeI y'GGCC_r
Cuts at: 1439 1814 2456 3329 3503 3894 3921 5266 1439
Size: 375 642 873 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 873 642 391 375 174 27
EagI C'GGCC_G
Cuts at: 3329 3329
Size: 5744
EarI CTCTTCn'nnn_
Cuts at: 442 715 1341 1453 1489 3767 3977 442
Size: 273 626 112 36 2278 210 2209
Fragments arranged by size:
2278 2209 626 273 210 112 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 3952 4262 4408 5554 2323
Size: 1629 310 146 1146 2513
Fragments arranged by size:
2513 1629 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2756 2756
Size: 5744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 890 1354 1462 3569 4001 4748 5016 722
Size: 168 464 108 2107 432 747 268 1450
Fragments arranged by size:
2107 1450 747 464 432 268 168 108
EcoO109I rG'GnC_Cy
Cuts at: 675 727 1158 1682 2235 2883 675
Size: 52 431 524 553 648 3536
Fragments arranged by size:
3536 648 553 524 431 52
EcoRI G'AATT_C
Cuts at: 1389 2748 1389
Size: 1359 4385
EcoRV GAT'ATC
Cuts at: 1857 1857
Size: 5744
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 861 3763 861
Size: 2902 2842
FspI TGC'GCA
Cuts at: 2002 3525 2002
Size: 1523 4221
HaeII r_GCGC'y
Cuts at: 895 2426 3178 3426 4448 5427 895
Size: 1531 752 248 1022 979 1212
Fragments arranged by size:
1531 1212 1022 979 752 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 835 867 916 948 3586 3616 3618 3648 835
Size: 32 49 32 2638 30 2 30 2931
Fragments arranged by size:
2931 2638 49 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2783 2783
Size: 5744
KasI G'GCGC_C
Cuts at: 891 3174 3422 891
Size: 2283 248 3213
Fragments arranged by size:
3213 2283 248
KpnI G_GTAC'C
Cuts at: 908 2478 2764 908
Size: 1570 286 3888
Fragments arranged by size:
3888 1570 286
MluI A'CGCG_T
Cuts at: 5177 5177
Size: 5744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1537 4415 4599 1537
Size: 2878 184 2682
Fragments arranged by size:
2878 2682 184
MscI TGG'CCA
Cuts at: 1441 1816 3505 1441
Size: 375 1689 3680
Fragments arranged by size:
3680 1689 375
MslI CAynn'nnrTG
Cuts at: 1285 1493 1807 2524 3733 3860 1285
Size: 208 314 717 1209 127 3169
Fragments arranged by size:
3169 1209 717 314 208 127
MspA1I CmG'CkG
Cuts at: 1705 2045 2472 2855 3169 3529 4542 4787 1705
Size: 340 427 383 314 360 1013 245 2662
Fragments arranged by size:
2662 1013 427 383 360 340 314 245
NaeI GCC'GGC
Cuts at: 3925 3925
Size: 5744
NarI GG'CG_CC
Cuts at: 892 3175 3423 892
Size: 2283 248 3213
Fragments arranged by size:
3213 2283 248
NcoI C'CATG_G
Cuts at: 125 326 2318 125
Size: 201 1992 3551
Fragments arranged by size:
3551 1992 201
NgoMIV G'CCGG_C
Cuts at: 3923 3923
Size: 5744
NspI r_CATG'y
Cuts at: 1556 2193 2797 3297 4204 1556
Size: 637 604 500 907 3096
Fragments arranged by size:
3096 907 637 604 500
PciI A'CATG_T
Cuts at: 2189 4200 2189
Size: 2011 3733
PflMI CCAn_nnn'nTGG
Cuts at: 1986 2493 1986
Size: 507 5237
PfoI T'CCnGG_A
Cuts at: 595 595
Size: 5744
PmeI GTTT'AAAC
Cuts at: 2743 2743
Size: 5744
PmlI CAC'GTG
Cuts at: 956 1863 956
Size: 907 4837
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2076 2314 2346 4883 4915 2044
Size: 32 238 32 2537 32 2873
Fragments arranged by size:
2873 2537 238 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 675 1682 2235 675
Size: 1007 553 4184
Fragments arranged by size:
4184 1007 553
PshAI GACnn'nnGTC
Cuts at: 1329 1957 1329
Size: 628 5116
PstI C_TGCA'G
Cuts at: 163 882 2246 2702 2791 3476 163
Size: 719 1364 456 89 685 2431
Fragments arranged by size:
2431 1364 719 685 456 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5744
PvuII CAG'CTG
Cuts at: 3169 3529 3169
Size: 360 5384
RsrII CG'GwC_CG
Cuts at: 3939 3939
Size: 5744
SacI G_AGCT'C
Cuts at: 2758 2758
Size: 5744
SacII CC_GC'GG
Cuts at: 2046 2473 2046
Size: 427 5317
SalI G'TCGA_C
Cuts at: 2781 2781
Size: 5744
SapI GCTCTTCn'nnn_
Cuts at: 715 1489 3767 3977 715
Size: 774 2278 210 2482
Fragments arranged by size:
2482 2278 774 210
SbfI CC_TGCA'GG
Cuts at: 2791 2791
Size: 5744
ScaI AGT'ACT
Cuts at: 5261 5261
Size: 5744
SfcI C'TryA_G
Cuts at: 33 159 878 2242 2698 2787 3472 4465 4656
Size: 126 719 1364 456 89 685 993 191
Cuts at: 4656 33
Size: 1121
Fragments arranged by size:
1364 1121 993 719 685 456 191 126
89
SfoI GGC'GCC
Cuts at: 893 3176 3424 893
Size: 2283 248 3213
Fragments arranged by size:
3213 2283 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5744
SmaI CCC'GGG
Cuts at: 2125 2766 2125
Size: 641 5103
SmlI C'TyrA_G
Cuts at: 448 2899 3814 4306 4568 448
Size: 2451 915 492 262 1624
Fragments arranged by size:
2451 1624 915 492 262
SnaBI TAC'GTA
Cuts at: 2736 2736
Size: 5744
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5744
SphI G_CATG'C
Cuts at: 2797 3297 2797
Size: 500 5244
StyI C'CwwG_G
Cuts at: 125 219 256 326 678 945 2318 2878 3232
Size: 94 37 70 352 267 1373 560 354
Cuts at: 3232 125
Size: 2637
Fragments arranged by size:
2637 1373 560 354 352 267 94 70
37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 457 2354 2593 3327 3460 3919 4128 457
Size: 1897 239 734 133 459 209 2073
Fragments arranged by size:
2073 1897 734 459 239 209 133
TatI w'GTAC_w
Cuts at: 5259 5259
Size: 5744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2642 2674 5261 5293 2178
Size: 32 432 32 2587 32 2629
Fragments arranged by size:
2629 2587 432 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3541 3541
Size: 5744
XbaI T'CTAG_A
Cuts at: 918 2775 918
Size: 1857 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1935 2309 3239 1935
Size: 374 930 4440
Fragments arranged by size:
4440 930 374
XmaI C'CCGG_G
Cuts at: 2123 2764 2123
Size: 641 5103
XmnI GAAnn'nnTTC
Cuts at: 2059 2059
Size: 5744
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BfrBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI