Restriction map for
pF1KSDA0048
(Circular) (Six-base) MAPSORT of: pF1KSDA0048.seq Check: 7758 from: 1 to: 4955
pF1KSDA0048 4955 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 885 1029 885
Size: 144 4811
AatII G_ACGT'C
Cuts at: 1723 1723
Size: 4955
AccI GT'mk_AC
Cuts at: 1166 1993 1166
Size: 827 4128
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 2780 3212 3959 4227 370
Size: 2410 432 747 268 1098
Fragments arranged by size:
2410 1098 747 432 268
AfeI AGC'GCT
Cuts at: 1882 1882
Size: 4955
AflIII A'CryG_T
Cuts at: 226 1621 2307 3411 4388 226
Size: 1395 686 1104 977 793
Fragments arranged by size:
1395 1104 977 793 686
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AleI CACnn'nnGTG
Cuts at: 682 682
Size: 4955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 1328
Size: 4955
AlwNI CAG_nnn'CTG
Cuts at: 1065 1488 2260 3827 1065
Size: 423 772 1567 2193
Fragments arranged by size:
2193 1567 772 423
ApaI G_GGCC'C
Cuts at: 786 786
Size: 4955
ApaLI G'TGCA_C
Cuts at: 3725 3725
Size: 4955
ApoI r'AATT_y
Cuts at: 1959 4308 4564 1959
Size: 2349 256 2350
Fragments arranged by size:
2350 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 1591 1975 1591
Size: 384 4571
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 586 1014 1604 1971 2385 2633 2668 586
Size: 428 590 367 414 248 35 2873
Fragments arranged by size:
2873 590 428 414 367 248 35
BanII G_rGCy'C
Cuts at: 786 973 1540 1820 1969 2999 786
Size: 187 567 280 149 1030 2742
Fragments arranged by size:
2742 1030 567 280 187 149
BbeI G_GCGC'C
Cuts at: 1608 2389 2637 1608
Size: 781 248 3926
Fragments arranged by size:
3926 781 248
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3614 4955 2853
Size: 761 1341 2853
Fragments arranged by size:
2853 1341 761
BclI T'GATC_A
Cuts at: 461 2475 461
Size: 2014 2941
BglI GCCn_nnn'nGGC
Cuts at: 1390 1390
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 890 1145 1534 2067 890
Size: 255 389 533 3778
Fragments arranged by size:
3778 533 389 255
Bme1580I G_kGCm'C
Cuts at: 786 1019 1737 2580 2673 3729 786
Size: 233 718 843 93 1056 2012
Fragments arranged by size:
2012 1056 843 718 233 93
BmrI ACTGGGnnnn_n'
Cuts at: 146 1380 1863 2167 2248 2583 4727 146
Size: 1234 483 304 81 335 2144 374
Fragments arranged by size:
2144 1234 483 374 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 4955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 443 1676 230
Size: 213 1233 3509
Fragments arranged by size:
3509 1233 213
Bpu10I CC'TnA_GC
Cuts at: 207 207
Size: 4955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 3010 3502 3800 2131
Size: 879 492 298 3286
Fragments arranged by size:
3286 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 1576 4659 910
Size: 666 3083 1206
Fragments arranged by size:
3083 1206 666
BsaAI yAC'GTr
Cuts at: 1622 1947 2308 1622
Size: 325 361 4269
Fragments arranged by size:
4269 361 325
BsaBI GATnn'nnATC
Cuts at: 6 574 2469 4506 6
Size: 568 1895 2037 455
Fragments arranged by size:
2037 1895 568 455
BsaHI Gr'CG_yC
Cuts at: 1605 1720 2386 2634 3336 4832 1605
Size: 115 666 248 702 1496 1728
Fragments arranged by size:
1728 1496 702 666 248 115
BsaWI w'CCGG_w
Cuts at: 1183 2144 2665 3343 3617 3764 4175 1183
Size: 961 521 678 274 147 411 1963
Fragments arranged by size:
1963 961 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1636 1666 1636
Size: 30 4925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 787 1905 787
Size: 1118 3837
BseYI C'CCAG_C
Cuts at: 194 869 1055 1221 1353 1586 2380 3138 3715
Size: 675 186 166 132 233 794 758 577
Cuts at: 3715 4211 194
Size: 496 938
Fragments arranged by size:
938 794 758 675 577 496 233 186
166 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1096 1213 1516 1602 1732 706
Size: 390 117 303 86 130 3929
Fragments arranged by size:
3929 390 303 130 117 86
BsiEI CG_ry'CG
Cuts at: 104 2497 2543 3751 104
Size: 2393 46 1208 1308
Fragments arranged by size:
2393 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1278 1820 1969 2747 2937 3729 1278
Size: 542 149 778 190 792 2504
Fragments arranged by size:
2504 792 778 542 190 149
BsmI GAATG_Cn'
Cuts at: 1383 1383
Size: 4955
BsmBI CGTCTCn'nnnn_
Cuts at: 895 895
Size: 4955
Bsp1286I G_dGCh'C
Cuts at: 786 973 1019 1278 1540 1737 1820 1969 2580
Size: 187 46 259 262 197 83 149 611
Cuts at: 2580 2673 2747 2937 2999 3729 786
Size: 93 74 190 62 730 2012
Fragments arranged by size:
2012 730 611 262 259 197 190 187
149 93 83 74 62 46
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 1886 4131 1886
Size: 2245 2710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 327 885 962 993 1029 2005 2219 2521
Size: 157 558 77 31 36 976 214 302
Cuts at: 2521 2902 170
Size: 381 2223
Fragments arranged by size:
2223 976 558 381 302 214 157 77
36 31
BsrBI CCG'CTC
Cuts at: 50 692 1761 3247 3301 4785 4950 50
Size: 642 1069 1486 54 1484 165 55
Fragments arranged by size:
1486 1484 1069 642 165 55 54
BsrDI GCAATG_nn'
Cuts at: 735 1415 2867 735
Size: 680 1452 2823
Fragments arranged by size:
2823 1452 680
BsrFI r'CCGG_y
Cuts at: 2953 3134 3343 2953
Size: 181 209 4565
Fragments arranged by size:
4565 209 181
BssSI C'ACGA_G
Cuts at: 760 3226 3584 760
Size: 2466 358 2131
Fragments arranged by size:
2466 2131 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1065 1231 1273 1616 2332 1065
Size: 166 42 343 716 3688
Fragments arranged by size:
3688 716 343 166 42
BstBI TT'CG_AA
Cuts at: 110 3316 110
Size: 3206 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1288 1288
Size: 4955
BstYI r'GATC_y
Cuts at: 1 569 1049 1133 1547 1980 2447 2470 2805
Size: 568 480 84 414 433 467 23 335
Cuts at: 2805 3051 4063 4149 4161 4507 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 568 480 467 449 433 414 346
335 246 86 84 23 12
BtgI C'CryG_G
Cuts at: 125 295 923 125
Size: 170 628 4157
Fragments arranged by size:
4157 628 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2905 3086 3249 2905
Size: 181 163 4611
Fragments arranged by size:
4611 181 163
BtsI GCAGTG_nn'
Cuts at: 134 2198 134
Size: 2064 2891
DraI TTT'AAA
Cuts at: 1954 1954
Size: 4955
DraIII CAC_nnn'GTG
Cuts at: 1810 1810
Size: 4955
DrdI GACnn_nn'nnGTC
Cuts at: 2661 3519 2661
Size: 858 4097
EaeI y'GGCC_r
Cuts at: 1574 2540 2714 3105 3132 4477 1574
Size: 966 174 391 27 1345 2052
Fragments arranged by size:
2052 1345 966 391 174 27
EagI C'GGCC_G
Cuts at: 2540 2540
Size: 4955
EarI CTCTTCn'nnn_
Cuts at: 847 1642 1789 2978 3188 847
Size: 795 147 1189 210 2614
Fragments arranged by size:
2614 1189 795 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3473 3619 4765 3163
Size: 310 146 1146 3353
Fragments arranged by size:
3353 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1818 1967 1818
Size: 149 4806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 370 443 1676 2780 3212 3959 4227 230
Size: 140 73 1233 1104 432 747 268 958
Fragments arranged by size:
1233 1104 958 747 432 268 140 73
EcoNI CCTnn'n_nnAGG
Cuts at: 1207 1207
Size: 4955
EcoO109I rG'GnC_Cy
Cuts at: 473 782 783 2094 473
Size: 309 1 1311 3334
Fragments arranged by size:
3334 1311 309 1
EcoRI G'AATT_C
Cuts at: 1959 1959
Size: 4955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 1740 2974 369
Size: 1371 1234 2350
Fragments arranged by size:
2350 1371 1234
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 413 1608 1884 2389 2637 3659 4638 413
Size: 1195 276 505 248 1022 979 730
Fragments arranged by size:
1195 1022 979 730 505 276 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 726 758 1143 1175 1636 1668 2797 2827 2829
Size: 32 385 32 461 32 1129 30 2
Cuts at: 2829 2859 726
Size: 30 2822
Fragments arranged by size:
2822 1129 461 385 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 713 753 1994 713
Size: 40 1241 3674
Fragments arranged by size:
3674 1241 40
KasI G'GCGC_C
Cuts at: 1604 2385 2633 1604
Size: 781 248 3926
Fragments arranged by size:
3926 781 248
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1845 3626 3810 1845
Size: 1781 184 2990
Fragments arranged by size:
2990 1781 184
MscI TGG'CCA
Cuts at: 2716 2716
Size: 4955
MslI CAynn'nnrTG
Cuts at: 682 1286 2944 3071 682
Size: 604 1658 127 2566
Fragments arranged by size:
2566 1658 604 127
MspA1I CmG'CkG
Cuts at: 297 432 925 991 1144 1231 1312 1399 1488
Size: 135 493 66 153 87 81 87 89
Cuts at: 1488 1554 2066 2380 2740 3753 3998 297
Size: 66 512 314 360 1013 245 1254
Fragments arranged by size:
1254 1013 512 493 360 314 245 153
135 89 87 87 81 66 66
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 1605 2386 2634 1605
Size: 781 248 3926
Fragments arranged by size:
3926 781 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4955
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NspI r_CATG'y
Cuts at: 230 1831 2008 2508 3415 230
Size: 1601 177 500 907 1770
Fragments arranged by size:
1770 1601 907 500 177
PciI A'CATG_T
Cuts at: 226 3411 226
Size: 3185 1770
PfoI T'CCnGG_A
Cuts at: 420 1208 420
Size: 788 4167
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PmlI CAC'GTG
Cuts at: 1622 1622
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4923
PpuMI rG'GwC_Cy
Cuts at: 473 473
Size: 4955
PspOMI G'GGCC_C
Cuts at: 782 782
Size: 4955
PstI C_TGCA'G
Cuts at: 167 1236 2002 2687 167
Size: 1069 766 685 2435
Fragments arranged by size:
2435 1069 766 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 432 991 1144 1231 1312 1399 1488 1554 2380
Size: 559 153 87 81 87 89 66 826
Cuts at: 2380 2740 432
Size: 360 2647
Fragments arranged by size:
2647 826 559 360 153 89 87 87
81 66
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1820 1969 1820
Size: 149 4806
SacII CC_GC'GG
Cuts at: 298 926 298
Size: 628 4327
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SanDI GG'GwC_CC
Cuts at: 473 473
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 847 2978 3188 847
Size: 2131 210 2614
Fragments arranged by size:
2614 2131 210
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SexAI A'CCwGG_T
Cuts at: 1925 1925
Size: 4955
SfcI C'TryA_G
Cuts at: 33 163 1232 1254 1998 2683 3676 3867 33
Size: 130 1069 22 744 685 993 191 1121
Fragments arranged by size:
1121 1069 993 744 685 191 130 22
SfoI GGC'GCC
Cuts at: 1606 2387 2635 1606
Size: 781 248 3926
Fragments arranged by size:
3926 781 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 1593 1977 1593
Size: 384 4571
SmlI C'TyrA_G
Cuts at: 2110 3025 3517 3779 2110
Size: 915 492 262 3286
Fragments arranged by size:
3286 915 492 262
SnaBI TAC'GTA
Cuts at: 1947 1947
Size: 4955
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 1831 2008 2508 1831
Size: 177 500 4278
Fragments arranged by size:
4278 500 177
StyI C'CwwG_G
Cuts at: 125 1022 2089 2443 125
Size: 897 1067 354 2637
Fragments arranged by size:
2637 1067 897 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 1558 1852 2538 2671 3130 3339 199
Size: 1359 294 686 133 459 209 1815
Fragments arranged by size:
1815 1359 686 459 294 209 133
TatI w'GTAC_w
Cuts at: 546 4470 546
Size: 3924 1031
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 4923
Tth111I GACn'n_nGTC
Cuts at: 2752 2752
Size: 4955
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 580 2450 580
Size: 1870 3085
XmaI C'CCGG_G
Cuts at: 1591 1975 1591
Size: 384 4571
XmnI GAAnn'nnTTC
Cuts at: 1798 1882 1798
Size: 84 4871
ZraI GAC'GTC
Cuts at: 1721 1721
Size: 4955
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspXI PsrI SfiI SgrAI
SrfI SspI StuI SwaI XhoI