Restriction map for
pF1KSDA0049
(Circular) (Six-base) MAPSORT of: pF1KSDA0049.seq Check: 2977 from: 1 to: 6035
pF1KSDA0049 6035 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1645 1645
Size: 6035
AccI GT'mk_AC
Cuts at: 1489 3073 1489
Size: 1584 4451
Acc65I G'GTAC_C
Cuts at: 3051 3051
Size: 6035
AclI AA'CG_TT
Cuts at: 5875 5875
Size: 6035
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 707 916 1267 2189 2438 2648 2885 2962 2971
Size: 209 351 922 249 210 237 77 9
Cuts at: 2971 3860 4292 5039 5307 707
Size: 889 432 747 268 1435
Fragments arranged by size:
1435 922 889 747 432 351 268 249
237 210 209 77 9
AfeI AGC'GCT
Cuts at: 326 326
Size: 6035
AflII C'TTAA_G
Cuts at: 328 328
Size: 6035
AflIII A'CryG_T
Cuts at: 3387 4491 5468 3387
Size: 1104 977 3954
Fragments arranged by size:
3954 1104 977
AgeI A'CCGG_T
Cuts at: 4423 4423
Size: 6035
AhdI GACnn_n'nnGTC
Cuts at: 1806 1806
Size: 6035
AleI CACnn'nnGTG
Cuts at: 1870 1870
Size: 6035
AlwNI CAG_nnn'CTG
Cuts at: 1803 2538 2671 2845 3340 4907 1803
Size: 735 133 174 495 1567 2931
Fragments arranged by size:
2931 1567 735 495 174 133
ApaLI G'TGCA_C
Cuts at: 4805 4805
Size: 6035
ApoI r'AATT_y
Cuts at: 167 1109 1122 1260 3039 5388 5644 167
Size: 942 13 138 1779 2349 256 558
Fragments arranged by size:
2349 1779 942 558 256 138 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 6035
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
AvaI C'yCGr_G
Cuts at: 1535 3055 1535
Size: 1520 4515
BamHI G'GATC_C
Cuts at: 513 3060 513
Size: 2547 3488
BanI G'GyrC_C
Cuts at: 380 2747 3051 3465 3713 3748 380
Size: 2367 304 414 248 35 2667
Fragments arranged by size:
2667 2367 414 304 248 35
BanII G_rGCy'C
Cuts at: 922 1147 1585 1766 2276 2599 2693 3049 4079
Size: 225 438 181 510 323 94 356 1030
Cuts at: 4079 922
Size: 2878
Fragments arranged by size:
2878 1030 510 438 356 323 225 181
94
BbeI G_GCGC'C
Cuts at: 3469 3717 3469
Size: 248 5787
BbsI GAAGACnn'nnnn_
Cuts at: 508 955 2054 508
Size: 447 1099 4489
Fragments arranged by size:
4489 1099 447
BbvCI CC'TCA_GC
Cuts at: 2213 2456 2213
Size: 243 5792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 509 543 509
Size: 34 6001
BciVI GTATCCnnnnn_n'
Cuts at: 303 3933 4694 6035 303
Size: 3630 761 1341 303
Fragments arranged by size:
3630 1341 761 303
BclI T'GATC_A
Cuts at: 1607 2673 3555 1607
Size: 1066 882 4087
Fragments arranged by size:
4087 1066 882
BglI GCCn_nnn'nGGC
Cuts at: 2887 2887
Size: 6035
BglII A'GATC_T
Cuts at: 3550 5587 3550
Size: 2037 3998
BlpI GC'TnA_GC
Cuts at: 3147 3147
Size: 6035
Bme1580I G_kGCm'C
Cuts at: 383 1859 2552 3660 3753 4809 383
Size: 1476 693 1108 93 1056 1609
Fragments arranged by size:
1609 1476 1108 1056 693 93
BmgBI CAC'GTC
Cuts at: 888 888
Size: 6035
BmrI ACTGGGnnnn_n'
Cuts at: 403 2058 3247 3328 3663 5807 403
Size: 1655 1189 81 335 2144 631
Fragments arranged by size:
2144 1655 1189 631 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4402 4402
Size: 6035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 658 976 1123 1165 1332 2425 2498 2792
Size: 39 318 147 42 167 1093 73 294
Cuts at: 2792 619
Size: 3862
Fragments arranged by size:
3862 1093 318 294 167 147 73 42
39
Bpu10I CC'TnA_GC
Cuts at: 2213 2456 2213
Size: 243 5792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 1345 1411 1599 2727 3211 4090 4582 4880
Size: 797 66 188 1128 484 879 492 298
Cuts at: 4880 548
Size: 1703
Fragments arranged by size:
1703 1128 879 797 492 484 298 188
66
BsaI GGTCTCn'nnnn_
Cuts at: 900 5739 900
Size: 4839 1196
BsaAI yAC'GTr
Cuts at: 3027 3388 3027
Size: 361 5674
BsaBI GATnn'nnATC
Cuts at: 6 3549 5586 6
Size: 3543 2037 455
Fragments arranged by size:
3543 2037 455
BsaHI Gr'CG_yC
Cuts at: 1503 3466 3714 4416 5912 1503
Size: 1963 248 702 1496 1626
Fragments arranged by size:
1963 1626 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3224 3745 4423 4697 4844 5255 3224
Size: 521 678 274 147 411 4004
Fragments arranged by size:
4004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1471 1501 2765 2795 1471
Size: 30 1264 30 4711
Fragments arranged by size:
4711 1264 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 912 947 1886 2209 2248 2251 2254 2263 2272
Size: 35 939 323 39 3 3 9 9
Cuts at: 2272 2275 2278 2699 912
Size: 3 3 421 4248
Fragments arranged by size:
4248 939 421 323 39 35 9 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 1470 2440 2461 2830 3460 4218 4795 5291 1470
Size: 970 21 369 630 758 577 496 2214
Fragments arranged by size:
2214 970 758 630 577 496 369 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1262 1367 1566 2831 544
Size: 718 105 199 1265 3748
Fragments arranged by size:
3748 1265 718 199 105
BsiEI CG_ry'CG
Cuts at: 104 3577 3623 4831 104
Size: 3473 46 1208 1308
Fragments arranged by size:
3473 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1766 2276 2504 2599 3049 3827 4017 4809 1766
Size: 510 228 95 450 778 190 792 2992
Fragments arranged by size:
2992 792 778 510 450 228 190 95
BsmBI CGTCTCn'nnnn_
Cuts at: 595 595
Size: 6035
Bsp1286I G_dGCh'C
Cuts at: 383 922 1147 1585 1766 1859 2276 2504 2552
Size: 539 225 438 181 93 417 228 48
Cuts at: 2552 2599 2693 3049 3660 3753 3827 4017 4079
Size: 47 94 356 611 93 74 190 62
Cuts at: 4079 4809 383
Size: 730 1609
Fragments arranged by size:
1609 730 611 539 438 417 356 228
225 190 181 94 93 93 74 62
48 47
BspEI T'CCGG_A
Cuts at: 5255 5255
Size: 6035
BspHI T'CATG_A
Cuts at: 5211 5211
Size: 6035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1645 1811 2958 3085 3299 3601 3982 1645
Size: 166 1147 127 214 302 381 3698
Fragments arranged by size:
3698 1147 381 302 214 166 127
BsrBI CCG'CTC
Cuts at: 50 4327 4381 5865 6030 50
Size: 4277 54 1484 165 55
Fragments arranged by size:
4277 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 2021 2765 3947 1463
Size: 558 744 1182 3551
Fragments arranged by size:
3551 1182 744 558
BsrFI r'CCGG_y
Cuts at: 1432 4033 4214 4423 1432
Size: 2601 181 209 3044
Fragments arranged by size:
3044 2601 209 181
BsrGI T'GTAC_A
Cuts at: 2375 2607 2375
Size: 232 5803
BssSI C'ACGA_G
Cuts at: 4306 4664 4306
Size: 358 5677
BstAPI GCAn_nnn'nTGC
Cuts at: 782 1038 3412 782
Size: 256 2374 3405
Fragments arranged by size:
3405 2374 256
BstBI TT'CG_AA
Cuts at: 110 4396 110
Size: 4286 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1528 1528
Size: 6035
BstYI r'GATC_y
Cuts at: 1 513 1560 1782 3060 3527 3550 3885 4131
Size: 512 1047 222 1278 467 23 335 246
Cuts at: 4131 5143 5229 5241 5587 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1278 1047 1012 512 467 449 346 335
246 222 86 23 12
BstZ17I GTA'TAC
Cuts at: 1490 1490
Size: 6035
BtgI C'CryG_G
Cuts at: 125 1131 125
Size: 1006 5029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3985 4166 4329 3985
Size: 181 163 5691
Fragments arranged by size:
5691 181 163
BtsI GCAGTG_nn'
Cuts at: 527 2584 2843 3278 527
Size: 2057 259 435 3284
Fragments arranged by size:
3284 2057 435 259
DraI TTT'AAA
Cuts at: 159 1974 3034 159
Size: 1815 1060 3160
Fragments arranged by size:
3160 1815 1060
DrdI GACnn_nn'nnGTC
Cuts at: 3741 4599 3741
Size: 858 5177
EaeI y'GGCC_r
Cuts at: 870 1434 3620 3794 4185 4212 5557 870
Size: 564 2186 174 391 27 1345 1348
Fragments arranged by size:
2186 1348 1345 564 391 174 27
EagI C'GGCC_G
Cuts at: 3620 3620
Size: 6035
EarI CTCTTCn'nnn_
Cuts at: 281 474 1571 1946 2722 4058 4268 281
Size: 193 1097 375 776 1336 210 2048
Fragments arranged by size:
2048 1336 1097 776 375 210 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4243 4553 4699 5845 4243
Size: 310 146 1146 4433
Fragments arranged by size:
4433 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1764 2274 2597 3047 1764
Size: 510 323 450 4752
Fragments arranged by size:
4752 510 450 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 658 707 916 976 1123 1165 1267 1332
Size: 39 49 209 60 147 42 102 65
Cuts at: 1332 2189 2425 2438 2498 2648 2792 2885 2962
Size: 857 236 13 60 150 144 93 77
Cuts at: 2962 2971 3860 4292 5039 5307 619
Size: 9 889 432 747 268 1347
Fragments arranged by size:
1347 889 857 747 432 268 236 209
150 147 144 102 93 77 65 60
60 49 42 39 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 274 274
Size: 6035
EcoO109I rG'GnC_Cy
Cuts at: 1800 2060 2820 3174 1800
Size: 260 760 354 4661
Fragments arranged by size:
4661 760 354 260
EcoRI G'AATT_C
Cuts at: 1122 3039 1122
Size: 1917 4118
EcoRV GAT'ATC
Cuts at: 216 216
Size: 6035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 343 1950 2194 4054 343
Size: 1607 244 1860 2324
Fragments arranged by size:
2324 1860 1607 244
FseI GG_CCGG'CC
Cuts at: 1436 1436
Size: 6035
FspI TGC'GCA
Cuts at: 3816 3816
Size: 6035
HaeII r_GCGC'y
Cuts at: 328 3469 3717 4739 5718 328
Size: 3141 248 1022 979 645
Fragments arranged by size:
3141 1022 979 645 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1824 2877 2909 3877 3907 3909 3939 1792
Size: 32 1053 32 968 30 2 30 3888
Fragments arranged by size:
3888 1053 968 32 32 30 30 2
HincII GTy'rAC
Cuts at: 624 3074 624
Size: 2450 3585
HindIII A'AGCT_T
Cuts at: 174 640 664 174
Size: 466 24 5545
Fragments arranged by size:
5545 466 24
HpaI GTT'AAC
Cuts at: 624 624
Size: 6035
KasI G'GCGC_C
Cuts at: 3465 3713 3465
Size: 248 5787
KpnI G_GTAC'C
Cuts at: 3055 3055
Size: 6035
MluI A'CGCG_T
Cuts at: 5468 5468
Size: 6035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1802 1826 4706 4890 1802
Size: 24 2880 184 2947
Fragments arranged by size:
2947 2880 184 24
MscI TGG'CCA
Cuts at: 872 3796 872
Size: 2924 3111
MslI CAynn'nnrTG
Cuts at: 1870 2615 2753 4024 4151 1870
Size: 745 138 1271 127 3754
Fragments arranged by size:
3754 1271 745 138 127
MspA1I CmG'CkG
Cuts at: 761 2455 2925 3146 3460 3820 4833 5078 761
Size: 1694 470 221 314 360 1013 245 1718
Fragments arranged by size:
1718 1694 1013 470 360 314 245 221
NaeI GCC'GGC
Cuts at: 1434 4216 1434
Size: 2782 3253
NarI GG'CG_CC
Cuts at: 3466 3714 3466
Size: 248 5787
NcoI C'CATG_G
Cuts at: 125 1131 125
Size: 1006 5029
NdeI CA'TA_TG
Cuts at: 867 867
Size: 6035
NgoMIV G'CCGG_C
Cuts at: 1432 4214 1432
Size: 2782 3253
NspI r_CATG'y
Cuts at: 1893 3088 3588 4495 1893
Size: 1195 500 907 3433
Fragments arranged by size:
3433 1195 907 500
PasI CC'CwG_GG
Cuts at: 2361 2361
Size: 6035
PciI A'CATG_T
Cuts at: 4491 4491
Size: 6035
PmeI GTTT'AAAC
Cuts at: 3034 3034
Size: 6035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5174 5206 5174
Size: 32 6003
PpuMI rG'GwC_Cy
Cuts at: 1800 2060 1800
Size: 260 5775
PshAI GACnn'nnGTC
Cuts at: 2139 2139
Size: 6035
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 638 670 638
Size: 32 6003
PstI C_TGCA'G
Cuts at: 362 522 2969 3082 3767 362
Size: 160 2447 113 685 2630
Fragments arranged by size:
2630 2447 685 160 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6035
PvuII CAG'CTG
Cuts at: 761 2455 2925 3460 3820 761
Size: 1694 470 535 360 2976
Fragments arranged by size:
2976 1694 535 470 360
RsrII CG'GwC_CG
Cuts at: 4230 4230
Size: 6035
SacI G_AGCT'C
Cuts at: 1766 2276 2599 3049 1766
Size: 510 323 450 4752
Fragments arranged by size:
4752 510 450 323
SalI G'TCGA_C
Cuts at: 3072 3072
Size: 6035
SanDI GG'GwC_CC
Cuts at: 2060 2060
Size: 6035
SapI GCTCTTCn'nnn_
Cuts at: 2722 4058 4268 2722
Size: 1336 210 4489
Fragments arranged by size:
4489 1336 210
SbfI CC_TGCA'GG
Cuts at: 2969 3082 2969
Size: 113 5922
ScaI AGT'ACT
Cuts at: 949 5552 949
Size: 4603 1432
SfcI C'TryA_G
Cuts at: 33 358 518 2965 3078 3763 4756 4947 33
Size: 325 160 2447 113 685 993 191 1121
Fragments arranged by size:
2447 1121 993 685 325 191 160 113
SfoI GGC'GCC
Cuts at: 3467 3715 3467
Size: 248 5787
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
SmaI CCC'GGG
Cuts at: 3057 3057
Size: 6035
SmlI C'TyrA_G
Cuts at: 328 563 1360 1426 1614 2742 3190 4105 4597
Size: 235 797 66 188 1128 448 915 492
Cuts at: 4597 4859 328
Size: 262 1504
Fragments arranged by size:
1504 1128 915 797 492 448 262 235
188 66
SnaBI TAC'GTA
Cuts at: 3027 3027
Size: 6035
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6035
SphI G_CATG'C
Cuts at: 3088 3588 3088
Size: 500 5535
StuI AGG'CCT
Cuts at: 2402 2402
Size: 6035
StyI C'CwwG_G
Cuts at: 125 686 1131 1475 2099 3169 3523 125
Size: 561 445 344 624 1070 354 2637
Fragments arranged by size:
2637 1070 624 561 445 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3618 3751 4210 4419 3618
Size: 133 459 209 5234
Fragments arranged by size:
5234 459 209 133
TatI w'GTAC_w
Cuts at: 947 2375 2607 5550 947
Size: 1428 232 2943 1432
Fragments arranged by size:
2943 1432 1428 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 786 818 2765 2797 5552 5584 786
Size: 32 1947 32 2755 32 1237
Fragments arranged by size:
2755 1947 1237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3832 3832
Size: 6035
XbaI T'CTAG_A
Cuts at: 3066 3066
Size: 6035
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 3530 2358
Size: 1172 4863
XmaI C'CCGG_G
Cuts at: 3055 3055
Size: 6035
XmnI GAAnn'nnTTC
Cuts at: 1655 2984 1655
Size: 1329 4706
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AlfI AloI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BssHII BstEII Bsu36I ClaI CspCI
DraIII FspAI MfeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PsiI PspOMI PspXI SacII SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI