Restriction map for
pF1KSDA0050
(Circular) (Six-base) MAPSORT of: pF1KSDA0050.seq Check: 5230 from: 1 to: 5357
pF1KSDA0050 5357 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2395 2395
Size: 5357
Acc65I G'GTAC_C
Cuts at: 620 2373 620
Size: 1753 3604
AclI AA'CG_TT
Cuts at: 5197 5197
Size: 5357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1197 2159 2246 3182 3614 4361 4629 1197
Size: 962 87 936 432 747 268 1925
Fragments arranged by size:
1925 962 936 747 432 268 87
AflII C'TTAA_G
Cuts at: 900 900
Size: 5357
AflIII A'CryG_T
Cuts at: 849 2709 3813 4790 849
Size: 1860 1104 977 1416
Fragments arranged by size:
1860 1416 1104 977
AgeI A'CCGG_T
Cuts at: 3745 3745
Size: 5357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1663 1695 1663
Size: 32 5325
AlwNI CAG_nnn'CTG
Cuts at: 459 595 983 2662 4229 459
Size: 136 388 1679 1567 1587
Fragments arranged by size:
1679 1587 1567 388 136
ApaI G_GGCC'C
Cuts at: 573 1622 1845 2053 573
Size: 1049 223 208 3877
Fragments arranged by size:
3877 1049 223 208
ApaLI G'TGCA_C
Cuts at: 4127 4127
Size: 5357
ApoI r'AATT_y
Cuts at: 370 813 2361 4710 4966 370
Size: 443 1548 2349 256 761
Fragments arranged by size:
2349 1548 761 443 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5357
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
AvaI C'yCGr_G
Cuts at: 611 1241 2129 2377 611
Size: 630 888 248 3591
Fragments arranged by size:
3591 888 630 248
BamHI G'GATC_C
Cuts at: 2382 2382
Size: 5357
BanI G'GyrC_C
Cuts at: 272 563 620 787 1727 2373 2787 3035 3070
Size: 291 57 167 940 646 414 248 35
Cuts at: 3070 272
Size: 2559
Fragments arranged by size:
2559 940 646 414 291 248 167 57
35
BanII G_rGCy'C
Cuts at: 347 573 1261 1300 1622 1808 1845 2053 2090
Size: 226 688 39 322 186 37 208 37
Cuts at: 2090 2130 2371 3401 347
Size: 40 241 1030 2303
Fragments arranged by size:
2303 1030 688 322 241 226 208 186
40 39 37 37
BbeI G_GCGC'C
Cuts at: 276 567 791 1731 2791 3039 276
Size: 291 224 940 1060 248 2594
Fragments arranged by size:
2594 1060 940 291 248 224
BciVI GTATCCnnnnn_n'
Cuts at: 3255 4016 5357 3255
Size: 761 1341 3255
Fragments arranged by size:
3255 1341 761
BclI T'GATC_A
Cuts at: 2877 2877
Size: 5357
BglI GCCn_nnn'nGGC
Cuts at: 577 577
Size: 5357
BglII A'GATC_T
Cuts at: 1574 2872 4909 1574
Size: 1298 2037 2022
Fragments arranged by size:
2037 2022 1298
BlpI GC'TnA_GC
Cuts at: 754 2314 2469 754
Size: 1560 155 3642
Fragments arranged by size:
3642 1560 155
Bme1580I G_kGCm'C
Cuts at: 253 573 636 1343 1622 1754 1845 2053 2982
Size: 320 63 707 279 132 91 208 929
Cuts at: 2982 3075 4131 253
Size: 93 1056 1479
Fragments arranged by size:
1479 1056 929 707 320 279 208 132
93 91 63
BmrI ACTGGGnnnn_n'
Cuts at: 256 762 786 1374 1932 2569 2650 2985 5129
Size: 506 24 588 558 637 81 335 2144
Cuts at: 5129 256
Size: 484
Fragments arranged by size:
2144 637 588 558 506 484 335 81
24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 1827 3724 626
Size: 1201 1897 2259
Fragments arranged by size:
2259 1897 1201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 993 1129 1990 547
Size: 446 136 861 3914
Fragments arranged by size:
3914 861 446 136
Bpu10I CC'TnA_GC
Cuts at: 1260 1260
Size: 5357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1809 2533 3412 3904 4202 142
Size: 1667 724 879 492 298 1297
Fragments arranged by size:
1667 1297 879 724 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 214 262 1505 5061 214
Size: 48 1243 3556 510
Fragments arranged by size:
3556 1243 510 48
BsaAI yAC'GTr
Cuts at: 852 1344 1668 2349 2710 852
Size: 492 324 681 361 3499
Fragments arranged by size:
3499 681 492 361 324
BsaBI GATnn'nnATC
Cuts at: 6 2871 4908 6
Size: 2865 2037 455
Fragments arranged by size:
2865 2037 455
BsaHI Gr'CG_yC
Cuts at: 273 564 788 1728 2293 2788 3036 3738 5234
Size: 291 224 940 565 495 248 702 1496
Cuts at: 5234 273
Size: 396
Fragments arranged by size:
1496 940 702 565 495 396 291 248
224
BsaWI w'CCGG_w
Cuts at: 1496 2546 3067 3745 4019 4166 4577 1496
Size: 1050 521 678 274 147 411 2276
Fragments arranged by size:
2276 1050 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1461 1491 1663 1693 1901 1931 1461
Size: 30 172 30 208 30 4887
Fragments arranged by size:
4887 208 172 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 763 898 1132 1993 163
Size: 600 135 234 861 3527
Fragments arranged by size:
3527 861 600 234 135
BseYI C'CCAG_C
Cuts at: 616 783 873 1551 1621 2782 3540 4117 4613
Size: 167 90 678 70 1161 758 577 496
Cuts at: 4613 616
Size: 1360
Fragments arranged by size:
1360 1161 758 678 577 496 167 90
70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1210 1816 2041 1053
Size: 157 606 225 4369
Fragments arranged by size:
4369 606 225 157
BsiEI CG_ry'CG
Cuts at: 104 2899 2945 4153 104
Size: 2795 46 1208 1308
Fragments arranged by size:
2795 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2371 3149 3339 4131 2371
Size: 778 190 792 3597
Fragments arranged by size:
3597 792 778 190
BsmI GAATG_Cn'
Cuts at: 1445 1445
Size: 5357
BsmBI CGTCTCn'nnnn_
Cuts at: 1069 2248 1069
Size: 1179 4178
Bsp1286I G_dGCh'C
Cuts at: 253 347 573 636 1261 1300 1343 1622 1754
Size: 94 226 63 625 39 43 279 132
Cuts at: 1754 1808 1845 2053 2090 2130 2371 2982 3075
Size: 54 37 208 37 40 241 611 93
Cuts at: 3075 3149 3339 3401 4131 253
Size: 74 190 62 730 1479
Fragments arranged by size:
1479 730 625 611 279 241 226 208
190 132 94 93 74 63 62 54
43 40 39 37 37
BspEI T'CCGG_A
Cuts at: 4577 4577
Size: 5357
BspHI T'CATG_A
Cuts at: 2328 4533 2328
Size: 2205 3152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2407 2621 2923 3304 2407
Size: 214 302 381 4460
Fragments arranged by size:
4460 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3649 3703 5187 5352 50
Size: 3599 54 1484 165 55
Fragments arranged by size:
3599 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1199 1380 3269 1199
Size: 181 1889 3287
Fragments arranged by size:
3287 1889 181
BsrFI r'CCGG_y
Cuts at: 758 3355 3536 3745 758
Size: 2597 181 209 2370
Fragments arranged by size:
2597 2370 209 181
BssSI C'ACGA_G
Cuts at: 821 3628 3986 821
Size: 2807 358 2192
Fragments arranged by size:
2807 2192 358
BstAPI GCAn_nnn'nTGC
Cuts at: 595 1136 2734 595
Size: 541 1598 3218
Fragments arranged by size:
3218 1598 541
BstBI TT'CG_AA
Cuts at: 110 3718 110
Size: 3608 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2166 2166
Size: 5357
BstYI r'GATC_y
Cuts at: 1 449 1574 1792 2382 2849 2872 3207 3453
Size: 448 1125 218 590 467 23 335 246
Cuts at: 3453 4465 4551 4563 4909 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1125 1012 590 467 449 448 346 335
246 218 86 23 12
BtgI C'CryG_G
Cuts at: 1598 1886 1905 2165 1598
Size: 288 19 260 4790
Fragments arranged by size:
4790 288 260 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2145 3307 3488 3651 2145
Size: 1162 181 163 3851
Fragments arranged by size:
3851 1162 181 163
BtsI GCAGTG_nn'
Cuts at: 436 1610 2600 436
Size: 1174 990 3193
Fragments arranged by size:
3193 1174 990
DraI TTT'AAA
Cuts at: 2356 2356
Size: 5357
DraIII CAC_nnn'GTG
Cuts at: 1347 1347
Size: 5357
DrdI GACnn_nn'nnGTC
Cuts at: 3063 3921 3063
Size: 858 4499
EaeI y'GGCC_r
Cuts at: 1271 1310 1719 1777 2076 2942 3116 3507 3534
Size: 39 409 58 299 866 174 391 27
Cuts at: 3534 4879 1271
Size: 1345 1749
Fragments arranged by size:
1749 1345 866 409 391 299 174 58
39 27
EagI C'GGCC_G
Cuts at: 2942 2942
Size: 5357
EarI CTCTTCn'nnn_
Cuts at: 578 823 946 1707 1883 1988 3380 3590 578
Size: 245 123 761 176 105 1392 210 2345
Fragments arranged by size:
2345 1392 761 245 210 176 123 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 3565 3875 4021 5167 370
Size: 3195 310 146 1146 560
Fragments arranged by size:
3195 1146 560 310 146
EcoICRI GAG'CTC
Cuts at: 2369 2369
Size: 5357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 993 1129 1197 1990 2159 2246 3182 3614
Size: 446 136 68 793 169 87 936 432
Cuts at: 3614 4361 4629 547
Size: 747 268 1275
Fragments arranged by size:
1275 936 793 747 446 432 268 169
136 87 68
EcoNI CCTnn'n_nnAGG
Cuts at: 463 1144 463
Size: 681 4676
EcoO109I rG'GnC_Cy
Cuts at: 910 1032 1618 1772 1818 1841 1842 2049 2148
Size: 122 586 154 46 23 1 207 99
Cuts at: 2148 2496 910
Size: 348 3771
Fragments arranged by size:
3771 586 348 207 154 122 99 46
23 1
EcoRI G'AATT_C
Cuts at: 813 2361 813
Size: 1548 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 5357
FspI TGC'GCA
Cuts at: 3138 3138
Size: 5357
HaeII r_GCGC'y
Cuts at: 276 567 791 1164 1731 2791 3039 4061 5040
Size: 291 224 373 567 1060 248 1022 979
Cuts at: 5040 276
Size: 593
Fragments arranged by size:
1060 1022 979 593 567 373 291 248
224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1105 1137 1901 1933 3199 3229 3231 3261 1105
Size: 32 764 32 1266 30 2 30 3201
Fragments arranged by size:
3201 1266 764 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1920 2396 1920
Size: 476 4881
HindIII A'AGCT_T
Cuts at: 897 897
Size: 5357
KasI G'GCGC_C
Cuts at: 272 563 787 1727 2787 3035 272
Size: 291 224 940 1060 248 2594
Fragments arranged by size:
2594 1060 940 291 248 224
KpnI G_GTAC'C
Cuts at: 624 2377 624
Size: 1753 3604
MluI A'CGCG_T
Cuts at: 4790 4790
Size: 5357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4028 4212 4028
Size: 184 5173
MscI TGG'CCA
Cuts at: 1273 1312 2078 3118 1273
Size: 39 766 1040 3512
Fragments arranged by size:
3512 1040 766 39
MslI CAynn'nnrTG
Cuts at: 1603 2164 3346 3473 1603
Size: 561 1182 127 3487
Fragments arranged by size:
3487 1182 561 127
MspA1I CmG'CkG
Cuts at: 804 1173 1625 1956 1972 2055 2468 2782 3142
Size: 369 452 331 16 83 413 314 360
Cuts at: 3142 4155 4400 804
Size: 1013 245 1761
Fragments arranged by size:
1761 1013 452 413 369 360 331 314
245 83 16
NaeI GCC'GGC
Cuts at: 760 3538 760
Size: 2778 2579
NarI GG'CG_CC
Cuts at: 273 564 788 1728 2788 3036 273
Size: 291 224 940 1060 248 2594
Fragments arranged by size:
2594 1060 940 291 248 224
NcoI C'CATG_G
Cuts at: 1598 1886 1905 2165 1598
Size: 288 19 260 4790
Fragments arranged by size:
4790 288 260 19
NgoMIV G'CCGG_C
Cuts at: 758 3536 758
Size: 2778 2579
NspI r_CATG'y
Cuts at: 2410 2910 3817 2410
Size: 500 907 3950
Fragments arranged by size:
3950 907 500
PasI CC'CwG_GG
Cuts at: 1293 1837 2235 1293
Size: 544 398 4415
Fragments arranged by size:
4415 544 398
PciI A'CATG_T
Cuts at: 3813 3813
Size: 5357
PflMI CCAn_nnn'nTGG
Cuts at: 396 459 1365 396
Size: 63 906 4388
Fragments arranged by size:
4388 906 63
PfoI T'CCnGG_A
Cuts at: 260 260
Size: 5357
PmeI GTTT'AAAC
Cuts at: 2356 2356
Size: 5357
PmlI CAC'GTG
Cuts at: 852 1344 852
Size: 492 4865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1663 1695 4496 4528 1663
Size: 32 2801 32 2492
Fragments arranged by size:
2801 2492 32 32
PpuMI rG'GwC_Cy
Cuts at: 1032 1818 2148 1032
Size: 786 330 4241
Fragments arranged by size:
4241 786 330
PshAI GACnn'nnGTC
Cuts at: 460 1061 460
Size: 601 4756
PspOMI G'GGCC_C
Cuts at: 569 1618 1841 2049 569
Size: 1049 223 208 3877
Fragments arranged by size:
3877 1049 223 208
PspXI vC'TCGA_Gb
Cuts at: 611 611
Size: 5357
PstI C_TGCA'G
Cuts at: 443 653 1172 2404 3089 443
Size: 210 519 1232 685 2711
Fragments arranged by size:
2711 1232 685 519 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5357
PvuII CAG'CTG
Cuts at: 804 1173 1625 1972 2782 3142 804
Size: 369 452 347 810 360 3019
Fragments arranged by size:
3019 810 452 369 360 347
RsrII CG'GwC_CG
Cuts at: 3552 3552
Size: 5357
SacI G_AGCT'C
Cuts at: 2371 2371
Size: 5357
SalI G'TCGA_C
Cuts at: 2394 2394
Size: 5357
SanDI GG'GwC_CC
Cuts at: 2148 2148
Size: 5357
SapI GCTCTTCn'nnn_
Cuts at: 3380 3590 3380
Size: 210 5147
SbfI CC_TGCA'GG
Cuts at: 2404 2404
Size: 5357
ScaI AGT'ACT
Cuts at: 4874 4874
Size: 5357
SfcI C'TryA_G
Cuts at: 33 439 649 1168 2400 3085 4078 4269 33
Size: 406 210 519 1232 685 993 191 1121
Fragments arranged by size:
1232 1121 993 685 519 406 210 191
SfoI GGC'GCC
Cuts at: 274 565 789 1729 2789 3037 274
Size: 291 224 940 1060 248 2594
Fragments arranged by size:
2594 1060 940 291 248 224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
SmaI CCC'GGG
Cuts at: 1243 2379 1243
Size: 1136 4221
SmlI C'TyrA_G
Cuts at: 157 611 900 1788 2129 2512 3427 3919 4181
Size: 454 289 888 341 383 915 492 262
Cuts at: 4181 157
Size: 1333
Fragments arranged by size:
1333 915 888 492 454 383 341 289
262
SnaBI TAC'GTA
Cuts at: 2349 2349
Size: 5357
SpeI A'CTAG_T
Cuts at: 67 1530 67
Size: 1463 3894
SphI G_CATG'C
Cuts at: 2410 2910 2410
Size: 500 4857
StuI AGG'CCT
Cuts at: 1648 1648
Size: 5357
StyI C'CwwG_G
Cuts at: 1428 1473 1598 1732 1886 1905 2165 2491 2845
Size: 45 125 134 154 19 260 326 354
Cuts at: 2845 1428
Size: 3940
Fragments arranged by size:
3940 354 326 260 154 134 125 45
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 865 2940 3073 3532 3741 442
Size: 423 2075 133 459 209 2058
Fragments arranged by size:
2075 2058 459 423 209 133
TatI w'GTAC_w
Cuts at: 1025 4872 1025
Size: 3847 1510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 1461 1493 1943 1975 4874 4906 158
Size: 32 1271 32 450 32 2899 32 609
Fragments arranged by size:
2899 1271 609 450 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 3154 2188
Size: 966 4391
XbaI T'CTAG_A
Cuts at: 417 2388 417
Size: 1971 3386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1366 1409 1471 2852 1366
Size: 43 62 1381 3871
Fragments arranged by size:
3871 1381 62 43
XhoI C'TCGA_G
Cuts at: 611 2129 611
Size: 1518 3839
XmaI C'CCGG_G
Cuts at: 1241 2377 1241
Size: 1136 4221
XmnI GAAnn'nnTTC
Cuts at: 1678 1678
Size: 5357
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PsiI PsrI SacII SexAI SfiI SgrAI
SrfI SspI SwaI ZraI