Restriction map for
pF1KSDA0057
(Circular) (Six-base) MAPSORT of: pF1KSDA0057.seq Check: 7675 from: 1 to: 4247
pF1KSDA0057 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 2072 2504 3251 3519 147
Size: 1925 432 747 268 875
Fragments arranged by size:
1925 875 747 432 268
AflIII A'CryG_T
Cuts at: 1599 2703 3680 1599
Size: 1104 977 2166
Fragments arranged by size:
2166 1104 977
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AhdI GACnn_n'nnGTC
Cuts at: 484 484
Size: 4247
AleI CACnn'nnGTG
Cuts at: 505 505
Size: 4247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1189 1221 1189
Size: 32 4215
AlwNI CAG_nnn'CTG
Cuts at: 866 1004 1552 3119 866
Size: 138 548 1567 1994
Fragments arranged by size:
1994 1567 548 138
ApaLI G'TGCA_C
Cuts at: 322 586 3017 322
Size: 264 2431 1552
Fragments arranged by size:
2431 1552 264
ApoI r'AATT_y
Cuts at: 686 1251 3600 3856 686
Size: 565 2349 256 1077
Fragments arranged by size:
2349 1077 565 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 967 1267 967
Size: 300 3947
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 1261 2291 1261
Size: 1030 3217
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 578 578
Size: 4247
BciVI GTATCCnnnnn_n'
Cuts at: 535 2145 2906 4247 535
Size: 1610 761 1341 535
Fragments arranged by size:
1610 1341 761 535
BclI T'GATC_A
Cuts at: 1767 1767
Size: 4247
BglI GCCn_nnn'nGGC
Cuts at: 764 919 1033 764
Size: 155 114 3978
Fragments arranged by size:
3978 155 114
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 326 590 1872 1965 3021 326
Size: 264 1282 93 1056 1552
Fragments arranged by size:
1552 1282 1056 264 93
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 760 2614 760
Size: 1854 2393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 755 685
Size: 70 4177
Bpu10I CC'TnA_GC
Cuts at: 653 754 653
Size: 101 4146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 1423 2302 2794 3092 1210
Size: 213 879 492 298 2365
Fragments arranged by size:
2365 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 310 3951 310
Size: 3641 606
BsaAI yAC'GTr
Cuts at: 525 1239 1600 525
Size: 714 361 3172
Fragments arranged by size:
3172 714 361
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 1678 1926 2628 4124 1678
Size: 248 702 1496 1801
Fragments arranged by size:
1801 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1189 1219 1189
Size: 30 4217
BseYI C'CCAG_C
Cuts at: 1064 1119 1672 2430 3007 3503 1064
Size: 55 553 758 577 496 1808
Fragments arranged by size:
1808 758 577 553 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 4247
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 218 326 590 1261 2039 2229 3021 218
Size: 108 264 671 778 190 792 1444
Fragments arranged by size:
1444 792 778 671 264 190 108
Bsp1286I G_dGCh'C
Cuts at: 218 326 590 1261 1872 1965 2039 2229 2291
Size: 108 264 671 611 93 74 190 62
Cuts at: 2291 3021 218
Size: 730 1444
Fragments arranged by size:
1444 730 671 611 264 190 108 93
74 62
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1297 1511 1813 2194 1297
Size: 214 302 381 3350
Fragments arranged by size:
3350 381 302 214
BsrBI CCG'CTC
Cuts at: 50 159 2539 2593 4077 4242 50
Size: 109 2380 54 1484 165 55
Fragments arranged by size:
2380 1484 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 2159
Size: 4247
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BssSI C'ACGA_G
Cuts at: 2518 2876 2518
Size: 358 3889
BstAPI GCAn_nnn'nTGC
Cuts at: 1004 1624 1004
Size: 620 3627
BstBI TT'CG_AA
Cuts at: 110 2608 110
Size: 2498 1749
BstEII G'GTnAC_C
Cuts at: 885 1165 885
Size: 280 3967
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 4247
BstYI r'GATC_y
Cuts at: 1 1272 1739 1762 2097 2343 3355 3441 3453
Size: 1271 467 23 335 246 1012 86 12
Cuts at: 3453 3799 1
Size: 346 449
Fragments arranged by size:
1271 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 341 341
Size: 4247
BtgI C'CryG_G
Cuts at: 125 1210 125
Size: 1085 3162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2197 2378 2541 2197
Size: 181 163 3903
Fragments arranged by size:
3903 181 163
BtsI GCAGTG_nn'
Cuts at: 1490 1490
Size: 4247
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 920 1832 2006 2397 2424 3769 920
Size: 912 174 391 27 1345 1398
Fragments arranged by size:
1398 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 166 811 829 901 2270 2480 166
Size: 645 18 72 1369 210 1933
Fragments arranged by size:
1933 1369 645 210 72 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 685 755 2072 2504 3251 3519 147
Size: 538 70 1317 432 747 268 875
Fragments arranged by size:
1317 875 747 538 432 268 70
EcoO109I rG'GnC_Cy
Cuts at: 345 1386 345
Size: 1041 3206
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 2266 279
Size: 1987 2260
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 1053 1681 1929 2951 3930 1053
Size: 628 248 1022 979 1370
Fragments arranged by size:
1370 1022 979 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 470 502 2089 2119 2121 2151 470
Size: 32 1587 30 2 30 2566
Fragments arranged by size:
2566 1587 32 30 30 2
HincII GTy'rAC
Cuts at: 1286 1286
Size: 4247
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2918 3102 2918
Size: 184 4063
MscI TGG'CCA
Cuts at: 922 2008 922
Size: 1086 3161
MslI CAynn'nnrTG
Cuts at: 188 295 505 1175 2236 2363 188
Size: 107 210 670 1061 127 2072
Fragments arranged by size:
2072 1061 670 210 127 107
MspA1I CmG'CkG
Cuts at: 483 627 1068 1358 1672 2032 3045 3290 483
Size: 144 441 290 314 360 1013 245 1440
Fragments arranged by size:
1440 1013 441 360 314 290 245 144
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 125 125
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NspI r_CATG'y
Cuts at: 394 586 1300 1800 2707 394
Size: 192 714 500 907 1934
Fragments arranged by size:
1934 907 714 500 192
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 841 873 1189 1221 3386 3418 841
Size: 32 316 32 2165 32 1670
Fragments arranged by size:
2165 1670 316 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 345 345
Size: 4247
PstI C_TGCA'G
Cuts at: 788 1294 1979 788
Size: 506 685 3056
Fragments arranged by size:
3056 685 506
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 483 627 1068 1672 2032 483
Size: 144 441 604 360 2698
Fragments arranged by size:
2698 604 441 360 144
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 166 2270 2480 166
Size: 2104 210 1933
Fragments arranged by size:
2104 1933 210
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 790 3764 790
Size: 2974 1273
SexAI A'CCwGG_T
Cuts at: 347 719 347
Size: 372 3875
SfcI C'TryA_G
Cuts at: 33 302 784 1290 1975 2968 3159 33
Size: 269 482 506 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 482 269 191
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 1269 1269
Size: 4247
SmlI C'TyrA_G
Cuts at: 1225 1402 2317 2809 3071 1225
Size: 177 915 492 262 2401
Fragments arranged by size:
2401 915 492 262 177
SnaBI TAC'GTA
Cuts at: 1239 1239
Size: 4247
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 394 1300 1800 394
Size: 906 500 2841
Fragments arranged by size:
2841 906 500
StuI AGG'CCT
Cuts at: 1036 1036
Size: 4247
StyI C'CwwG_G
Cuts at: 125 1381 1735 125
Size: 1256 354 2637
Fragments arranged by size:
2637 1256 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1143 1830 1963 2422 2631 1143
Size: 687 133 459 209 2759
Fragments arranged by size:
2759 687 459 209 133
TatI w'GTAC_w
Cuts at: 407 788 3762 407
Size: 381 2974 892
Fragments arranged by size:
2974 892 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4215
Tth111I GACn'n_nGTC
Cuts at: 349 2044 349
Size: 1695 2552
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 563 1742 563
Size: 1179 3068
XmaI C'CCGG_G
Cuts at: 1267 1267
Size: 4247
XmnI GAAnn'nnTTC
Cuts at: 610 856 610
Size: 246 4001
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI