Restriction map for
pF1KSDA0062
(Circular) (Six-base) MAPSORT of: pF1KSDA0062.seq Check: 4847 from: 1 to: 4613
pF1KSDA0062 4613 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1651 1651
Size: 4613
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 2438 2870 3617 3885 331
Size: 2107 432 747 268 1059
Fragments arranged by size:
2107 1059 747 432 268
AfeI AGC'GCT
Cuts at: 243 573 243
Size: 330 4283
AflII C'TTAA_G
Cuts at: 859 859
Size: 4613
AflIII A'CryG_T
Cuts at: 1965 3069 4046 1965
Size: 1104 977 2532
Fragments arranged by size:
2532 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AleI CACnn'nnGTG
Cuts at: 1234 1234
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 309 498 1068 1533 1918 3485 309
Size: 189 570 465 385 1567 1437
Fragments arranged by size:
1567 1437 570 465 385 189
ApaLI G'TGCA_C
Cuts at: 227 3383 227
Size: 3156 1457
ApoI r'AATT_y
Cuts at: 1617 3966 4222 1617
Size: 2349 256 2008
Fragments arranged by size:
2349 2008 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 506 1633 506
Size: 1127 3486
BamHI G'GATC_C
Cuts at: 1638 1638
Size: 4613
BanI G'GyrC_C
Cuts at: 648 1017 1629 2043 2291 2326 648
Size: 369 612 414 248 35 2935
Fragments arranged by size:
2935 612 414 369 248 35
BanII G_rGCy'C
Cuts at: 467 1051 1627 2657 467
Size: 584 576 1030 2423
Fragments arranged by size:
2423 1030 584 576
BbeI G_GCGC'C
Cuts at: 1021 2047 2295 1021
Size: 1026 248 3339
Fragments arranged by size:
3339 1026 248
BbsI GAAGACnn'nnnn_
Cuts at: 670 670
Size: 4613
BbvCI CC'TCA_GC
Cuts at: 986 986
Size: 4613
BciVI GTATCCnnnnn_n'
Cuts at: 583 2511 3272 4613 583
Size: 1928 761 1341 583
Fragments arranged by size:
1928 1341 761 583
BclI T'GATC_A
Cuts at: 1142 2133 1142
Size: 991 3622
BglII A'GATC_T
Cuts at: 842 2128 4165 842
Size: 1286 2037 1290
Fragments arranged by size:
2037 1290 1286
BlpI GC'TnA_GC
Cuts at: 1725 1725
Size: 4613
Bme1580I G_kGCm'C
Cuts at: 231 653 2238 2331 3387 231
Size: 422 1585 93 1056 1457
Fragments arranged by size:
1585 1457 1056 422 93
BmrI ACTGGGnnnn_n'
Cuts at: 1825 1906 2241 4385 1825
Size: 81 335 2144 2053
Fragments arranged by size:
2144 2053 335 81
BmtI G_CTAG'C
Cuts at: 1420 1420
Size: 4613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 451 475 1442 428
Size: 23 24 967 3599
Fragments arranged by size:
3599 967 24 23
Bpu10I CC'TnA_GC
Cuts at: 986 986
Size: 4613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1789 2668 3160 3458 1789
Size: 879 492 298 2944
Fragments arranged by size:
2944 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 404 601 4317 404
Size: 197 3716 700
Fragments arranged by size:
3716 700 197
BsaAI yAC'GTr
Cuts at: 369 395 1605 1966 369
Size: 26 1210 361 3016
Fragments arranged by size:
3016 1210 361 26
BsaBI GATnn'nnATC
Cuts at: 6 1306 2127 4164 6
Size: 1300 821 2037 455
Fragments arranged by size:
2037 1300 821 455
BsaHI Gr'CG_yC
Cuts at: 1018 2044 2292 2994 4490 1018
Size: 1026 248 702 1496 1141
Fragments arranged by size:
1496 1141 1026 702 248
BsaWI w'CCGG_w
Cuts at: 1802 2323 3001 3275 3422 3833 1802
Size: 521 678 274 147 411 2582
Fragments arranged by size:
2582 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1265 1295 1265
Size: 30 4583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 553 571 589 949 1267 553
Size: 18 18 360 318 3899
Fragments arranged by size:
3899 360 318 18 18
BseYI C'CCAG_C
Cuts at: 362 1297 2038 2796 3373 3869 362
Size: 935 741 758 577 496 1106
Fragments arranged by size:
1106 935 758 741 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 498 1081 127
Size: 371 583 3659
Fragments arranged by size:
3659 583 371
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3409 104
Size: 2051 46 1208 1308
Fragments arranged by size:
2051 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 231 467 1627 2405 2595 3387 231
Size: 236 1160 778 190 792 1457
Fragments arranged by size:
1457 1160 792 778 236 190
Bsp1286I G_dGCh'C
Cuts at: 231 467 653 1051 1627 2238 2331 2405 2595
Size: 236 186 398 576 611 93 74 190
Cuts at: 2595 2657 3387 231
Size: 62 730 1457
Fragments arranged by size:
1457 730 611 576 398 236 190 186
93 74 62
BspEI T'CCGG_A
Cuts at: 3833 3833
Size: 4613
BspHI T'CATG_A
Cuts at: 656 3789 656
Size: 3133 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1076 1663 1877 2179 2560 1076
Size: 587 214 302 381 3129
Fragments arranged by size:
3129 587 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 2959 4443 4608 50
Size: 2855 54 1484 165 55
Fragments arranged by size:
2855 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2525 2525
Size: 4613
BsrFI r'CCGG_y
Cuts at: 1381 2611 2792 3001 1381
Size: 1230 181 209 2993
Fragments arranged by size:
2993 1230 209 181
BssSI C'ACGA_G
Cuts at: 2884 3242 2884
Size: 358 4255
BstAPI GCAn_nnn'nTGC
Cuts at: 1068 1990 1068
Size: 922 3691
BstBI TT'CG_AA
Cuts at: 110 2974 110
Size: 2864 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1563 1563
Size: 4613
BstYI r'GATC_y
Cuts at: 1 261 842 1589 1638 2105 2128 2463 2709
Size: 260 581 747 49 467 23 335 246
Cuts at: 2709 3721 3807 3819 4165 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 747 581 467 449 346 335 260
246 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 203 203
Size: 4613
BtgI C'CryG_G
Cuts at: 1022 1022
Size: 4613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2563 2744 2907 2563
Size: 181 163 4269
Fragments arranged by size:
4269 181 163
BtsI GCAGTG_nn'
Cuts at: 419 806 990 1005 1856 419
Size: 387 184 15 851 3176
Fragments arranged by size:
3176 851 387 184 15
ClaI AT'CG_AT
Cuts at: 1175 1175
Size: 4613
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 1036 2319 3177 1036
Size: 1283 858 2472
Fragments arranged by size:
2472 1283 858
EaeI y'GGCC_r
Cuts at: 565 1184 1238 1379 2198 2372 2763 2790 4135
Size: 619 54 141 819 174 391 27 1345
Cuts at: 4135 565
Size: 1043
Fragments arranged by size:
1345 1043 819 619 391 174 141 54
27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 421 739 1327 2636 2846 421
Size: 318 588 1309 210 2188
Fragments arranged by size:
2188 1309 588 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 1625 465
Size: 1160 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 428 451 475 1442 2438 2870 3617 3885
Size: 97 23 24 967 996 432 747 268
Cuts at: 3885 331
Size: 1059
Fragments arranged by size:
1059 996 967 747 432 268 97 24
23
EcoNI CCTnn'n_nnAGG
Cuts at: 1249 1249
Size: 4613
EcoO109I rG'GnC_Cy
Cuts at: 315 966 1065 1752 315
Size: 651 99 687 3176
Fragments arranged by size:
3176 687 651 99
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
EcoRV GAT'ATC
Cuts at: 1122 1122
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 684 2632 684
Size: 1948 2665
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 245 575 1021 2047 2295 3317 4296 245
Size: 330 446 1026 248 1022 979 562
Fragments arranged by size:
1026 1022 979 562 446 330 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 581 613 1131 1163 1265 1297 1541 1573 2455
Size: 32 518 32 102 32 244 32 882
Cuts at: 2455 2485 2487 2517 581
Size: 30 2 30 2677
Fragments arranged by size:
2677 882 518 244 102 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1652 1652
Size: 4613
KasI G'GCGC_C
Cuts at: 1017 2043 2291 1017
Size: 1026 248 3339
Fragments arranged by size:
3339 1026 248
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 1335 3284 3468 749
Size: 586 1949 184 1894
Fragments arranged by size:
1949 1894 586 184
MscI TGG'CCA
Cuts at: 1186 1240 2374 1186
Size: 54 1134 3425
Fragments arranged by size:
3425 1134 54
MslI CAynn'nnrTG
Cuts at: 275 332 881 1234 1558 1561 2602 2729 275
Size: 57 549 353 324 3 1041 127 2159
Fragments arranged by size:
2159 1041 549 353 324 127 57 3
MspA1I CmG'CkG
Cuts at: 309 498 1724 2038 2398 3411 3656 309
Size: 189 1226 314 360 1013 245 1266
Fragments arranged by size:
1266 1226 1013 360 314 245 189
NaeI GCC'GGC
Cuts at: 1383 2794 1383
Size: 1411 3202
NarI GG'CG_CC
Cuts at: 1018 2044 2292 1018
Size: 1026 248 3339
Fragments arranged by size:
3339 1026 248
NcoI C'CATG_G
Cuts at: 1022 1022
Size: 4613
NgoMIV G'CCGG_C
Cuts at: 1381 2792 1381
Size: 1411 3202
NheI G'CTAG_C
Cuts at: 1416 1416
Size: 4613
NspI r_CATG'y
Cuts at: 1666 2166 3073 1666
Size: 500 907 3206
Fragments arranged by size:
3206 907 500
PasI CC'CwG_GG
Cuts at: 222 222
Size: 4613
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 498 1533 498
Size: 1035 3578
PfoI T'CCnGG_A
Cuts at: 467 746 467
Size: 279 4334
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PmlI CAC'GTG
Cuts at: 369 395 369
Size: 26 4587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 966 1065 966
Size: 99 4514
PstI C_TGCA'G
Cuts at: 260 305 801 959 997 1073 1660 2345 260
Size: 45 496 158 38 76 587 685 2528
Fragments arranged by size:
2528 685 587 496 158 76 45 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 309 498 2038 2398 309
Size: 189 1540 360 2524
Fragments arranged by size:
2524 1540 360 189
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 467 1627 467
Size: 1160 3453
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 260 1073 1660 260
Size: 813 587 3213
Fragments arranged by size:
3213 813 587
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 256 301 797 955 993 1069 1656 2341
Size: 223 45 496 158 38 76 587 685
Cuts at: 2341 3334 3525 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 587 496 223 191 158
76 45 38
SfoI GGC'GCC
Cuts at: 1019 2045 2293 1019
Size: 1026 248 3339
Fragments arranged by size:
3339 1026 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 508 1635 508
Size: 1127 3486
SmlI C'TyrA_G
Cuts at: 859 1768 2683 3175 3437 859
Size: 909 915 492 262 2035
Fragments arranged by size:
2035 915 909 492 262
SnaBI TAC'GTA
Cuts at: 1605 1605
Size: 4613
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1666 2166 1666
Size: 500 4113
StyI C'CwwG_G
Cuts at: 1022 1218 1747 2101 1022
Size: 196 529 354 3534
Fragments arranged by size:
3534 529 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 376 2196 2329 2788 2997 214
Size: 162 1820 133 459 209 1830
Fragments arranged by size:
1830 1820 459 209 162 133
TatI w'GTAC_w
Cuts at: 4128 4128
Size: 4613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 4130 4162 202
Size: 32 3896 32 653
Fragments arranged by size:
3896 653 32 32
Tth111I GACn'n_nGTC
Cuts at: 1279 2410 1279
Size: 1131 3482
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 798 2108 798
Size: 1310 3303
XmaI C'CCGG_G
Cuts at: 506 1633 506
Size: 1127 3486
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI