Restriction map for
pF1KSDA0063
(Circular) (Six-base) MAPSORT of: pF1KSDA0063.seq Check: 4975 from: 1 to: 3745
pF1KSDA0063 3745 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3745
Acc65I G'GTAC_C
Cuts at: 687 759 687
Size: 72 3673
AclI AA'CG_TT
Cuts at: 3585 3585
Size: 3745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1568 2000 2749 3017 382
Size: 1186 432 749 268 1110
Fragments arranged by size:
1186 1110 749 432 268
AflIII A'CryG_T
Cuts at: 1095 2201 3178 1095
Size: 1106 977 1662
Fragments arranged by size:
1662 1106 977
AgeI A'CCGG_T
Cuts at: 2133 2133
Size: 3745
AleI CACnn'nnGTG
Cuts at: 328 328
Size: 3745
AlwNI CAG_nnn'CTG
Cuts at: 1048 2617 1048
Size: 1569 2176
ApaLI G'TGCA_C
Cuts at: 2515 2515
Size: 3745
ApoI r'AATT_y
Cuts at: 747 3098 3354 747
Size: 2351 256 1138
Fragments arranged by size:
2351 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3745
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3745
AvaI C'yCGr_G
Cuts at: 281 604 763 281
Size: 323 159 3263
Fragments arranged by size:
3263 323 159
BamHI G'GATC_C
Cuts at: 768 768
Size: 3745
BanI G'GyrC_C
Cuts at: 687 759 1173 1421 1456 687
Size: 72 414 248 35 2976
Fragments arranged by size:
2976 414 248 72 35
BanII G_rGCy'C
Cuts at: 632 757 1787 632
Size: 125 1030 2590
Fragments arranged by size:
2590 1030 125
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3497
BbsI GAAGACnn'nnnn_
Cuts at: 249 249
Size: 3745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 460 426
Size: 34 3711
BciVI GTATCCnnnnn_n'
Cuts at: 279 1641 2404 3745 279
Size: 1362 763 1341 279
Fragments arranged by size:
1362 1341 763 279
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3745
BglII A'GATC_T
Cuts at: 1258 3297 1258
Size: 2039 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3745
Bme1580I G_kGCm'C
Cuts at: 237 1368 1461 2519 237
Size: 1131 93 1058 1463
Fragments arranged by size:
1463 1131 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3517 955
Size: 81 335 2146 1183
Fragments arranged by size:
2146 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 332 2110 332
Size: 1778 1967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 480
Size: 3745
Bpu10I CC'TnA_GC
Cuts at: 631 631
Size: 3745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 919 1798 2292 2590 580
Size: 339 879 494 298 1735
Fragments arranged by size:
1735 879 494 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 3449 3449
Size: 3745
BsaAI yAC'GTr
Cuts at: 735 1096 735
Size: 361 3384
BsaBI GATnn'nnATC
Cuts at: 6 1257 3296 6
Size: 1251 2039 455
Fragments arranged by size:
2039 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2126 3622 1174
Size: 248 704 1496 1297
Fragments arranged by size:
1496 1297 704 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2133 2407 2554 2965 932
Size: 521 680 274 147 411 1712
Fragments arranged by size:
1712 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 571 601 571
Size: 30 3715
BseYI C'CCAG_C
Cuts at: 354 506 1168 1926 2505 3001 354
Size: 152 662 758 579 496 1098
Fragments arranged by size:
1098 758 662 579 496 152
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2541 104
Size: 1181 46 1210 1308
Fragments arranged by size:
1308 1210 1181 46
BsiHKAI G_wGCw'C
Cuts at: 632 757 1535 1725 2519 632
Size: 125 778 190 794 1858
Fragments arranged by size:
1858 794 778 190 125
Bsp1286I G_dGCh'C
Cuts at: 237 632 757 1368 1461 1535 1725 1787 2519
Size: 395 125 611 93 74 190 62 732
Cuts at: 2519 237
Size: 1463
Fragments arranged by size:
1463 732 611 395 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 2965 2965
Size: 3745
BspHI T'CATG_A
Cuts at: 482 2921 482
Size: 2439 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1007 1309 1690 793
Size: 214 302 381 2848
Fragments arranged by size:
2848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3575 3740 50
Size: 1985 54 1486 165 55
Fragments arranged by size:
1985 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 276 452 1655 276
Size: 176 1203 2366
Fragments arranged by size:
2366 1203 176
BsrFI r'CCGG_y
Cuts at: 1741 1922 2133 1741
Size: 181 211 3353
Fragments arranged by size:
3353 211 181
BssSI C'ACGA_G
Cuts at: 2014 2374 2014
Size: 360 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3745
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3745
BstYI r'GATC_y
Cuts at: 1 545 768 1235 1258 1593 1839 2853 2939
Size: 544 223 467 23 335 246 1014 86
Cuts at: 2939 2951 3297 1
Size: 12 346 449
Fragments arranged by size:
1014 544 467 449 346 335 246 223
86 23 12
BtgI C'CryG_G
Cuts at: 139 326 139
Size: 187 3558
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3401
Fragments arranged by size:
3401 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3745
DraI TTT'AAA
Cuts at: 742 742
Size: 3745
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2309 1449
Size: 860 2885
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3267 1328
Size: 174 391 27 1347 1806
Fragments arranged by size:
1806 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3745
EarI CTCTTCn'nnn_
Cuts at: 340 689 1766 1976 340
Size: 349 1077 210 2109
Fragments arranged by size:
2109 1077 349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1951 2263 2409 3555 1951
Size: 312 146 1146 2141
Fragments arranged by size:
2141 1146 312 146
EcoICRI GAG'CTC
Cuts at: 630 755 630
Size: 125 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 480 1568 2000 2749 3017 382
Size: 98 1088 432 749 268 1110
Fragments arranged by size:
1110 1088 749 432 268 98
EcoO109I rG'GnC_Cy
Cuts at: 569 882 569
Size: 313 3432
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3745
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3745
HaeII r_GCGC'y
Cuts at: 1177 1425 2449 3428 1177
Size: 248 1024 979 1494
Fragments arranged by size:
1494 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1585 1615 1617 1647 1585
Size: 30 2 30 3683
Fragments arranged by size:
3683 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3745
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3497
KpnI G_GTAC'C
Cuts at: 691 763 691
Size: 72 3673
MluI A'CGCG_T
Cuts at: 3178 3178
Size: 3745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 697 2416 2600 697
Size: 1719 184 1842
Fragments arranged by size:
1842 1719 184
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3745
MslI CAynn'nnrTG
Cuts at: 328 1732 1859 328
Size: 1404 127 2214
Fragments arranged by size:
2214 1404 127
MspA1I CmG'CkG
Cuts at: 854 1168 1528 2543 2788 854
Size: 314 360 1015 245 1811
Fragments arranged by size:
1811 1015 360 314 245
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3745
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3497
NcoI C'CATG_G
Cuts at: 139 326 139
Size: 187 3558
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3745
NruI TCG'CGA
Cuts at: 2106 2106
Size: 3745
NspI r_CATG'y
Cuts at: 354 796 1296 2205 354
Size: 442 500 909 1894
Fragments arranged by size:
1894 909 500 442
PciI A'CATG_T
Cuts at: 2201 2201
Size: 3745
PfoI T'CCnGG_A
Cuts at: 260 260
Size: 3745
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2916 2884
Size: 32 3713
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3060
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3745
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3385
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3745
SacI G_AGCT'C
Cuts at: 632 757 632
Size: 125 3620
SalI G'TCGA_C
Cuts at: 780 780
Size: 3745
SapI GCTCTTCn'nnn_
Cuts at: 340 1766 1976 340
Size: 1426 210 2109
Fragments arranged by size:
2109 1426 210
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3745
ScaI AGT'ACT
Cuts at: 3262 3262
Size: 3745
SfcI C'TryA_G
Cuts at: 33 786 1471 2466 2657 33
Size: 753 685 995 191 1121
Fragments arranged by size:
1121 995 753 685 191
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3745
SmaI CCC'GGG
Cuts at: 283 765 283
Size: 482 3263
SmlI C'TyrA_G
Cuts at: 595 898 1813 2307 2569 595
Size: 303 915 494 262 1771
Fragments arranged by size:
1771 915 494 303 262
SnaBI TAC'GTA
Cuts at: 735 735
Size: 3745
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3745
SphI G_CATG'C
Cuts at: 354 796 1296 354
Size: 442 500 2803
Fragments arranged by size:
2803 500 442
StyI C'CwwG_G
Cuts at: 139 326 877 1231 139
Size: 187 551 354 2653
Fragments arranged by size:
2653 551 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 739 1326 1459 1918 2129 739
Size: 587 133 459 211 2355
Fragments arranged by size:
2355 587 459 211 133
TatI w'GTAC_w
Cuts at: 3260 3260
Size: 3745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3262 3294 3262
Size: 32 3713
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3745
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3745
XmaI C'CCGG_G
Cuts at: 281 763 281
Size: 482 3263
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI