Restriction map for
pF1KSDA0064
(Circular) (Six-base) MAPSORT of: pF1KSDA0064.seq Check: 8983 from: 1 to: 4547
pF1KSDA0064 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 2372 2804 3551 3819 515
Size: 1857 432 747 268 1243
Fragments arranged by size:
1857 1243 747 432 268
AflIII A'CryG_T
Cuts at: 1899 3003 3980 1899
Size: 1104 977 2466
Fragments arranged by size:
2466 1104 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 246 921 1852 3419 246
Size: 675 931 1567 1374
Fragments arranged by size:
1567 1374 931 675
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 1400 1567 1400
Size: 167 4380
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 591 624 591
Size: 33 4514
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 1563 1977 2225 2260 1563
Size: 414 248 35 3850
Fragments arranged by size:
3850 414 248 35
BanII G_rGCy'C
Cuts at: 1016 1561 2591 1016
Size: 545 1030 2972
Fragments arranged by size:
2972 1030 545
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbvCI CC'TCA_GC
Cuts at: 335 335
Size: 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 398 364
Size: 34 4513
BciVI GTATCCnnnnn_n'
Cuts at: 592 2445 3206 4547 592
Size: 1853 761 1341 592
Fragments arranged by size:
1853 1341 761 592
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 187 958 1037 187
Size: 771 79 3697
Fragments arranged by size:
3697 771 79
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 493 1015 1659 493
Size: 522 644 3381
Fragments arranged by size:
3381 644 522
Bme1580I G_kGCm'C
Cuts at: 441 2172 2265 3321 441
Size: 1731 93 1056 1667
Fragments arranged by size:
1731 1667 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1109 1759 1840 2175 4319 1109
Size: 650 81 335 2144 1337
Fragments arranged by size:
2144 1337 650 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 903 2914 903
Size: 2011 2536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 574
Size: 4547
Bpu10I CC'TnA_GC
Cuts at: 335 727 335
Size: 392 4155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 1407 1723 2602 3094 3392 1402
Size: 5 316 879 492 298 2557
Fragments arranged by size:
2557 879 492 316 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 4251 4251
Size: 4547
BsaAI yAC'GTr
Cuts at: 186 204 1539 1900 186
Size: 18 1335 361 2833
Fragments arranged by size:
2833 1335 361 18
BsaBI GATnn'nnATC
Cuts at: 6 1243 1529 2061 4098 6
Size: 1237 286 532 2037 455
Fragments arranged by size:
2037 1237 532 455 286
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 1089 363
Size: 726 3821
BseYI C'CCAG_C
Cuts at: 1465 1972 2730 3307 3803 1465
Size: 507 758 577 496 2209
Fragments arranged by size:
2209 758 577 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 238 202
Size: 36 4511
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1016 1561 2339 2529 3321 1016
Size: 545 778 190 792 2242
Fragments arranged by size:
2242 792 778 545 190
BsmI GAATG_Cn'
Cuts at: 298 298
Size: 4547
BsmBI CGTCTCn'nnnn_
Cuts at: 1332 1332
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 441 1016 1561 2172 2265 2339 2529 2591 3321
Size: 575 545 611 93 74 190 62 730
Cuts at: 3321 441
Size: 1667
Fragments arranged by size:
1667 730 611 575 545 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 1184 1235 3723 1184
Size: 51 2488 2008
Fragments arranged by size:
2488 2008 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 447 800 1597 1811 2113 2494 447
Size: 353 797 214 302 381 2500
Fragments arranged by size:
2500 797 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 2839 2893 4377 4542 50
Size: 2789 54 1484 165 55
Fragments arranged by size:
2789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 394 1108 2459 394
Size: 714 1351 2482
Fragments arranged by size:
2482 1351 714
BsrFI r'CCGG_y
Cuts at: 857 1196 1316 2545 2726 2935 857
Size: 339 120 1229 181 209 2469
Fragments arranged by size:
2469 1229 339 209 181 120
BssSI C'ACGA_G
Cuts at: 256 1125 2818 3176 256
Size: 869 1693 358 1627
Fragments arranged by size:
1693 1627 869 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1082 1924 1082
Size: 842 3705
BstBI TT'CG_AA
Cuts at: 110 2908 110
Size: 2798 1749
BstEII G'GTnAC_C
Cuts at: 840 1068 1092 1209 840
Size: 228 24 117 4178
Fragments arranged by size:
4178 228 117 24
BstYI r'GATC_y
Cuts at: 1 576 833 1530 1572 2039 2062 2397 2643
Size: 575 257 697 42 467 23 335 246
Cuts at: 2643 3655 3741 3753 4099 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 697 575 467 449 346 335 257
246 86 42 23 12
BtgI C'CryG_G
Cuts at: 1259 1494 1259
Size: 235 4312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1095 2497 2678 2841 1095
Size: 1402 181 163 2801
Fragments arranged by size:
2801 1402 181 163
BtsI GCAGTG_nn'
Cuts at: 1367 1375 1790 1367
Size: 8 415 4124
Fragments arranged by size:
4124 415 8
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 1038 2132 2306 2697 2724 4069 1038
Size: 1094 174 391 27 1345 1516
Fragments arranged by size:
1516 1345 1094 391 174 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 727 2570 2780 727
Size: 1843 210 2494
Fragments arranged by size:
2494 1843 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 1018 2755 3065 3211 4357 168
Size: 850 1737 310 146 1146 358
Fragments arranged by size:
1737 1146 850 358 310 146
EcoICRI GAG'CTC
Cuts at: 1014 1559 1014
Size: 545 4002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 574 2372 2804 3551 3819 515
Size: 59 1798 432 747 268 1243
Fragments arranged by size:
1798 1243 747 432 268 59
EcoO109I rG'GnC_Cy
Cuts at: 459 1686 459
Size: 1227 3320
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 1981 2229 3251 4230 1981
Size: 248 1022 979 2298
Fragments arranged by size:
2298 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 2389 2419 2421 2451 460
Size: 32 1897 30 2 30 2556
Fragments arranged by size:
2556 1897 32 30 30 2
HincII GTy'rAC
Cuts at: 519 1586 519
Size: 1067 3480
HindIII A'AGCT_T
Cuts at: 313 313
Size: 4547
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3218 3402 3218
Size: 184 4363
MscI TGG'CCA
Cuts at: 1040 2308 1040
Size: 1268 3279
MslI CAynn'nnrTG
Cuts at: 202 206 1075 2536 2663 202
Size: 4 869 1461 127 2086
Fragments arranged by size:
2086 1461 869 127 4
MspA1I CmG'CkG
Cuts at: 161 170 1658 1972 2332 3345 3590 161
Size: 9 1488 314 360 1013 245 1118
Fragments arranged by size:
1488 1118 1013 360 314 245 9
NaeI GCC'GGC
Cuts at: 1318 2728 1318
Size: 1410 3137
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 1259 1259
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 1316 2726 1316
Size: 1410 3137
NspI r_CATG'y
Cuts at: 375 1080 1600 2100 3007 375
Size: 705 520 500 907 1915
Fragments arranged by size:
1915 907 705 520 500
PciI A'CATG_T
Cuts at: 3003 3003
Size: 4547
PflMI CCAn_nnn'nTGG
Cuts at: 246 400 246
Size: 154 4393
PfoI T'CCnGG_A
Cuts at: 551 551
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PmlI CAC'GTG
Cuts at: 204 204
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 459 459
Size: 4547
PstI C_TGCA'G
Cuts at: 1025 1594 2279 1025
Size: 569 685 3293
Fragments arranged by size:
3293 685 569
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1972 2332 1972
Size: 360 4187
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1016 1561 1016
Size: 545 4002
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 234 560 1021 1201 1590 2275 3268 3459
Size: 201 326 461 180 389 685 993 191
Cuts at: 3459 33
Size: 1121
Fragments arranged by size:
1121 993 685 461 389 326 201 191
180
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SgrAI Cr'CCGG_yG
Cuts at: 1316 1316
Size: 4547
SmaI CCC'GGG
Cuts at: 1402 1569 1402
Size: 167 4380
SmlI C'TyrA_G
Cuts at: 1381 1422 1702 2617 3109 3371 1381
Size: 41 280 915 492 262 2557
Fragments arranged by size:
2557 915 492 280 262 41
SnaBI TAC'GTA
Cuts at: 1539 1539
Size: 4547
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1080 1600 2100 1080
Size: 520 500 3527
Fragments arranged by size:
3527 520 500
StyI C'CwwG_G
Cuts at: 479 845 1259 1681 2035 479
Size: 366 414 422 354 2991
Fragments arranged by size:
2991 422 414 366 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 818 2130 2263 2722 2931 818
Size: 1312 133 459 209 2434
Fragments arranged by size:
2434 1312 459 209 133
TatI w'GTAC_w
Cuts at: 368 4062 368
Size: 3694 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 443 475 4064 4096 443
Size: 32 3589 32 894
Fragments arranged by size:
3589 894 32 32
Tth111I GACn'n_nGTC
Cuts at: 763 2344 763
Size: 1581 2966
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2042 2042
Size: 4547
XmaI C'CCGG_G
Cuts at: 1400 1567 1400
Size: 167 4380
XmnI GAAnn'nnTTC
Cuts at: 316 898 316
Size: 582 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbsI BfrBI BmgBI BmtI BsaXI BsiWI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SrfI SspI
StuI SwaI XhoI ZraI