Restriction map for
pF1KSDA0065
(Circular) (Six-base) MAPSORT of: pF1KSDA0065.seq Check: 9053 from: 1 to: 5567
pF1KSDA0065 5567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2605 2605
Size: 5567
Acc65I G'GTAC_C
Cuts at: 2583 2583
Size: 5567
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1063 2211 3392 3824 4571 4839 294
Size: 769 1148 1181 432 747 268 1022
Fragments arranged by size:
1181 1148 1022 769 747 432 268
AflIII A'CryG_T
Cuts at: 2919 4023 5000 2919
Size: 1104 977 3486
Fragments arranged by size:
3486 1104 977
AgeI A'CCGG_T
Cuts at: 3955 3955
Size: 5567
AleI CACnn'nnGTG
Cuts at: 1514 1514
Size: 5567
AlwNI CAG_nnn'CTG
Cuts at: 210 2872 4439 210
Size: 2662 1567 1338
Fragments arranged by size:
2662 1567 1338
ApaLI G'TGCA_C
Cuts at: 1171 4337 1171
Size: 3166 2401
ApoI r'AATT_y
Cuts at: 352 628 874 949 1589 1801 2009 2221 2472
Size: 276 246 75 640 212 208 212 251
Cuts at: 2472 2571 4920 5176 352
Size: 99 2349 256 743
Fragments arranged by size:
2349 743 640 276 256 251 246 212
212 208 99 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 2587 2587
Size: 5567
BamHI G'GATC_C
Cuts at: 2592 2592
Size: 5567
BanI G'GyrC_C
Cuts at: 2583 2997 3245 3280 2583
Size: 414 248 35 4870
Fragments arranged by size:
4870 414 248 35
BanII G_rGCy'C
Cuts at: 231 2265 2581 3611 231
Size: 2034 316 1030 2187
Fragments arranged by size:
2187 2034 1030 316
BbeI G_GCGC'C
Cuts at: 3001 3249 3001
Size: 248 5319
BbsI GAAGACnn'nnnn_
Cuts at: 2436 2436
Size: 5567
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4226 5567 3465
Size: 761 1341 3465
Fragments arranged by size:
3465 1341 761
BclI T'GATC_A
Cuts at: 387 3087 387
Size: 2700 2867
BfrBI ATG'CAT
Cuts at: 1624 1624
Size: 5567
BglII A'GATC_T
Cuts at: 3082 5119 3082
Size: 2037 3530
BlpI GC'TnA_GC
Cuts at: 2679 2679
Size: 5567
Bme1580I G_kGCm'C
Cuts at: 1175 3192 3285 4341 1175
Size: 2017 93 1056 2401
Fragments arranged by size:
2401 2017 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2779 2860 3195 5339 2779
Size: 81 335 2144 3007
Fragments arranged by size:
3007 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 3934
Size: 5567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 5567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 1558 2743 3622 4114 4412 668
Size: 890 1185 879 492 298 1823
Fragments arranged by size:
1823 1185 890 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5271 5271
Size: 5567
BsaAI yAC'GTr
Cuts at: 2559 2920 2559
Size: 361 5206
BsaBI GATnn'nnATC
Cuts at: 6 3081 5118 6
Size: 3075 2037 455
Fragments arranged by size:
3075 2037 455
BsaHI Gr'CG_yC
Cuts at: 2998 3246 3948 5444 2998
Size: 248 702 1496 3121
Fragments arranged by size:
3121 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2756 3277 3955 4229 4376 4787 2756
Size: 521 678 274 147 411 3536
Fragments arranged by size:
3536 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 417 748 211
Size: 206 331 5030
Fragments arranged by size:
5030 331 206
BseYI C'CCAG_C
Cuts at: 2992 3750 4327 4823 2992
Size: 758 577 496 3736
Fragments arranged by size:
3736 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3109 3155 4363 104
Size: 3005 46 1208 1308
Fragments arranged by size:
3005 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 773 1175 2581 3359 3549 4341 773
Size: 402 1406 778 190 792 1999
Fragments arranged by size:
1999 1406 792 778 402 190
BsmI GAATG_Cn'
Cuts at: 1285 2209 1285
Size: 924 4643
Bsp1286I G_dGCh'C
Cuts at: 231 773 1175 2265 2581 3192 3285 3359 3549
Size: 542 402 1090 316 611 93 74 190
Cuts at: 3549 3611 4341 231
Size: 62 730 1457
Fragments arranged by size:
1457 1090 730 611 542 402 316 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4787 4787
Size: 5567
BspHI T'CATG_A
Cuts at: 729 1872 4743 729
Size: 1143 2871 1553
Fragments arranged by size:
2871 1553 1143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2617 2831 3133 3514 2617
Size: 214 302 381 4670
Fragments arranged by size:
4670 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3859 3913 5397 5562 50
Size: 3809 54 1484 165 55
Fragments arranged by size:
3809 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1290 2214 3479 1290
Size: 924 1265 3378
Fragments arranged by size:
3378 1265 924
BsrFI r'CCGG_y
Cuts at: 3565 3746 3955 3565
Size: 181 209 5177
Fragments arranged by size:
5177 209 181
BsrGI T'GTAC_A
Cuts at: 2082 2418 2082
Size: 336 5231
BssSI C'ACGA_G
Cuts at: 3838 4196 3838
Size: 358 5209
BstAPI GCAn_nnn'nTGC
Cuts at: 2944 2944
Size: 5567
BstBI TT'CG_AA
Cuts at: 110 3928 110
Size: 3818 1749
BstYI r'GATC_y
Cuts at: 1 2592 3059 3082 3417 3663 4675 4761 4773
Size: 2591 467 23 335 246 1012 86 12
Cuts at: 4773 5119 1
Size: 346 449
Fragments arranged by size:
2591 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 334 334
Size: 5567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5223
Fragments arranged by size:
5223 181 163
BtsI GCAGTG_nn'
Cuts at: 2810 2810
Size: 5567
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1272 1307 2196 2231 1272
Size: 35 889 35 4608
Fragments arranged by size:
4608 889 35 35
DraI TTT'AAA
Cuts at: 168 2566 168
Size: 2398 3169
DraIII CAC_nnn'GTG
Cuts at: 2521 2521
Size: 5567
DrdI GACnn_nn'nnGTC
Cuts at: 3273 4131 3273
Size: 858 4709
EaeI y'GGCC_r
Cuts at: 3152 3326 3717 3744 5089 3152
Size: 174 391 27 1345 3630
Fragments arranged by size:
3630 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3152 3152
Size: 5567
EarI CTCTTCn'nnn_
Cuts at: 323 829 845 3590 3800 323
Size: 506 16 2745 210 2090
Fragments arranged by size:
2745 2090 506 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3775 4085 4231 5377 3775
Size: 310 146 1146 3965
Fragments arranged by size:
3965 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2579 2579
Size: 5567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 294 1063 2211 3392 3824 4571 4839 271
Size: 23 769 1148 1181 432 747 268 999
Fragments arranged by size:
1181 1148 999 769 747 432 268 23
EcoNI CCTnn'n_nnAGG
Cuts at: 811 811
Size: 5567
EcoO109I rG'GnC_Cy
Cuts at: 2706 2706
Size: 5567
EcoRI G'AATT_C
Cuts at: 352 1589 2009 2571 352
Size: 1237 420 562 3348
Fragments arranged by size:
3348 1237 562 420
EcoRV GAT'ATC
Cuts at: 923 923
Size: 5567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 5567
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 3001 3249 4271 5250 3001
Size: 248 1022 979 3318
Fragments arranged by size:
3318 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 372 404 886 918 3409 3439 3441
Size: 32 195 32 482 32 2491 30 2
Cuts at: 3441 3471 145
Size: 30 2241
Fragments arranged by size:
2491 2241 482 195 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1820 2606 1820
Size: 786 4781
HindIII A'AGCT_T
Cuts at: 366 1130 1214 2054 366
Size: 764 84 840 3879
Fragments arranged by size:
3879 840 764 84
KasI G'GCGC_C
Cuts at: 2997 3245 2997
Size: 248 5319
KpnI G_GTAC'C
Cuts at: 2587 2587
Size: 5567
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4238 4422 4238
Size: 184 5383
MscI TGG'CCA
Cuts at: 3328 3328
Size: 5567
MslI CAynn'nnrTG
Cuts at: 1514 1877 3556 3683 1514
Size: 363 1679 127 3398
Fragments arranged by size:
3398 1679 363 127
MspA1I CmG'CkG
Cuts at: 385 2678 2992 3352 4365 4610 385
Size: 2293 314 360 1013 245 1342
Fragments arranged by size:
2293 1342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3748 3748
Size: 5567
NarI GG'CG_CC
Cuts at: 2998 3246 2998
Size: 248 5319
NcoI C'CATG_G
Cuts at: 334 334
Size: 5567
NgoMIV G'CCGG_C
Cuts at: 3746 3746
Size: 5567
NsiI A_TGCA'T
Cuts at: 1626 1626
Size: 5567
NspI r_CATG'y
Cuts at: 1628 1787 2384 2620 3120 4027 1628
Size: 159 597 236 500 907 3168
Fragments arranged by size:
3168 907 597 500 236 159
PciI A'CATG_T
Cuts at: 4023 4023
Size: 5567
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 4706
Size: 32 5535
PshAI GACnn'nnGTC
Cuts at: 218 218
Size: 5567
PsiI TTA'TAA
Cuts at: 869 869
Size: 5567
PstI C_TGCA'G
Cuts at: 2614 3299 2614
Size: 685 4882
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 385 2992 3352 385
Size: 2607 360 2600
Fragments arranged by size:
2607 2600 360
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 2581 2581
Size: 5567
SalI G'TCGA_C
Cuts at: 2604 2604
Size: 5567
SapI GCTCTTCn'nnn_
Cuts at: 323 3590 3800 323
Size: 3267 210 2090
Fragments arranged by size:
3267 2090 210
SbfI CC_TGCA'GG
Cuts at: 2614 2614
Size: 5567
ScaI AGT'ACT
Cuts at: 5084 5084
Size: 5567
SfcI C'TryA_G
Cuts at: 33 258 2610 3295 4288 4479 33
Size: 225 2352 685 993 191 1121
Fragments arranged by size:
2352 1121 993 685 225 191
SfoI GGC'GCC
Cuts at: 2999 3247 2999
Size: 248 5319
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 2589 2589
Size: 5567
SmlI C'TyrA_G
Cuts at: 683 1537 2722 3637 4129 4391 683
Size: 854 1185 915 492 262 1859
Fragments arranged by size:
1859 1185 915 854 492 262
SnaBI TAC'GTA
Cuts at: 2559 2559
Size: 5567
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 2620 3120 2620
Size: 500 5067
SspI AAT'ATT
Cuts at: 660 660
Size: 5567
StyI C'CwwG_G
Cuts at: 334 2701 3055 334
Size: 2367 354 2846
Fragments arranged by size:
2846 2367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 3150 3283 3742 3951 205
Size: 2945 133 459 209 1821
Fragments arranged by size:
2945 1821 459 209 133
TatI w'GTAC_w
Cuts at: 1494 1746 2082 2250 2418 5082 1494
Size: 252 336 168 168 2664 1979
Fragments arranged by size:
2664 1979 336 252 168 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5084 5116 5084
Size: 32 5535
Tth111I GACn'n_nGTC
Cuts at: 3364 3364
Size: 5567
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3062
Size: 5567
XmaI C'CCGG_G
Cuts at: 2587 2587
Size: 5567
XmnI GAAnn'nnTTC
Cuts at: 1048 1132 1636 1720 2224 2392 1048
Size: 84 504 84 504 168 4223
Fragments arranged by size:
4223 504 504 168 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI