Restriction map for
pF1KSDA0067
(Circular) (Six-base) MAPSORT of: pF1KSDA0067.seq Check: 7108 from: 1 to: 7010
pF1KSDA0067 7010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1503 1521 1528 1503
Size: 18 7 6985
Fragments arranged by size:
6985 18 7
AatII G_ACGT'C
Cuts at: 3993 3993
Size: 7010
AccI GT'mk_AC
Cuts at: 846 2445 2774 4048 846
Size: 1599 329 1274 3808
Fragments arranged by size:
3808 1599 1274 329
Acc65I G'GTAC_C
Cuts at: 4026 4026
Size: 7010
AclI AA'CG_TT
Cuts at: 6850 6850
Size: 7010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 2840 2856 3356 3566 4835 5267 6014 6282
Size: 2637 16 500 210 1269 432 747 268
Cuts at: 6282 203
Size: 931
Fragments arranged by size:
2637 1269 931 747 500 432 268 210
16
AflII C'TTAA_G
Cuts at: 594 594
Size: 7010
AflIII A'CryG_T
Cuts at: 1378 2495 4362 5466 6443 1378
Size: 1117 1867 1104 977 1945
Fragments arranged by size:
1945 1867 1117 1104 977
AgeI A'CCGG_T
Cuts at: 1441 2507 5398 1441
Size: 1066 2891 3053
Fragments arranged by size:
3053 2891 1066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1303 1335 2516 2548 1303
Size: 32 1181 32 5765
Fragments arranged by size:
5765 1181 32 32
AlwNI CAG_nnn'CTG
Cuts at: 663 1345 1437 1618 1729 2293 4315 5882 663
Size: 682 92 181 111 564 2022 1567 1791
Fragments arranged by size:
2022 1791 1567 682 564 181 111 92
ApaI G_GGCC'C
Cuts at: 1800 1800
Size: 7010
ApaLI G'TGCA_C
Cuts at: 2499 5780 2499
Size: 3281 3729
ApoI r'AATT_y
Cuts at: 734 823 2296 4014 6363 6619 734
Size: 89 1473 1718 2349 256 1125
Fragments arranged by size:
2349 1718 1473 1125 256 89
AseI AT'TA_AT
Cuts at: 20 20
Size: 7010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7010
AvaI C'yCGr_G
Cuts at: 1220 1877 2262 2918 3617 4030 1220
Size: 657 385 656 699 413 4200
Fragments arranged by size:
4200 699 657 656 413 385
BamHI G'GATC_C
Cuts at: 1253 2106 4035 1253
Size: 853 1929 4228
Fragments arranged by size:
4228 1929 853
BanI G'GyrC_C
Cuts at: 772 4026 4440 4688 4723 772
Size: 3254 414 248 35 3059
Fragments arranged by size:
3254 3059 414 248 35
BanII G_rGCy'C
Cuts at: 1316 1627 1800 1811 4024 5054 1316
Size: 311 173 11 2213 1030 3272
Fragments arranged by size:
3272 2213 1030 311 173 11
BbeI G_GCGC'C
Cuts at: 4444 4692 4444
Size: 248 6762
BbsI GAAGACnn'nnnn_
Cuts at: 122 1106 1767 3390 3822 3985 122
Size: 984 661 1623 432 163 3147
Fragments arranged by size:
3147 1623 984 661 432 163
BbvCI CC'TCA_GC
Cuts at: 1338 1743 1338
Size: 405 6605
BciVI GTATCCnnnnn_n'
Cuts at: 1067 2269 2584 3831 4908 5669 7010 1067
Size: 1202 315 1247 1077 761 1341 1067
Fragments arranged by size:
1341 1247 1202 1077 1067 761 315
BclI T'GATC_A
Cuts at: 4530 4530
Size: 7010
BfrBI ATG'CAT
Cuts at: 379 3372 379
Size: 2993 4017
BglII A'GATC_T
Cuts at: 2976 4525 6562 2976
Size: 1549 2037 3424
Fragments arranged by size:
3424 2037 1549
BlpI GC'TnA_GC
Cuts at: 4122 4122
Size: 7010
Bme1580I G_kGCm'C
Cuts at: 1800 2503 4635 4728 5784 1800
Size: 703 2132 93 1056 3026
Fragments arranged by size:
3026 2132 1056 703 93
BmgBI CAC'GTC
Cuts at: 1381 1601 1381
Size: 220 6790
BmrI ACTGGGnnnn_n'
Cuts at: 232 1449 1563 1765 4222 4303 4638 6782 232
Size: 1217 114 202 2457 81 335 2144 460
Fragments arranged by size:
2457 2144 1217 460 335 202 114 81
BmtI G_CTAG'C
Cuts at: 310 310
Size: 7010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 572 3213 5377 572
Size: 2641 2164 2205
Fragments arranged by size:
2641 2205 2164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 713 1330 1372 1745 1810 2110 289
Size: 424 617 42 373 65 300 5189
Fragments arranged by size:
5189 617 424 373 300 65 42
Bpu10I CC'TnA_GC
Cuts at: 997 1338 1372 1743 997
Size: 341 34 371 6264
Fragments arranged by size:
6264 371 341 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 699 1153 4186 5065 5557 5855 593
Size: 106 454 3033 879 492 298 1748
Fragments arranged by size:
3033 1748 879 492 454 298 106
BsaI GGTCTCn'nnnn_
Cuts at: 2026 6714 2026
Size: 4688 2322
BsaAI yAC'GTr
Cuts at: 1208 4002 4363 1208
Size: 2794 361 3855
Fragments arranged by size:
3855 2794 361
BsaBI GATnn'nnATC
Cuts at: 6 938 4524 6561 6
Size: 932 3586 2037 455
Fragments arranged by size:
3586 2037 932 455
BsaHI Gr'CG_yC
Cuts at: 1968 3990 4441 4689 5391 6887 1968
Size: 2022 451 248 702 1496 2091
Fragments arranged by size:
2091 2022 1496 702 451 248
BsaWI w'CCGG_w
Cuts at: 1441 2507 4199 4720 5398 5672 5819 6230 1441
Size: 1066 1692 521 678 274 147 411 2221
Fragments arranged by size:
2221 1692 1066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 409 439 1303 1333 2211 2241 409
Size: 30 864 30 878 30 5178
Fragments arranged by size:
5178 878 864 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1141 1243 1416 3751 1141
Size: 102 173 2335 4400
Fragments arranged by size:
4400 2335 173 102
BseYI C'CCAG_C
Cuts at: 432 544 1734 1800 2567 3166 3895 4435 5193
Size: 112 1190 66 767 599 729 540 758
Cuts at: 5193 5770 6266 432
Size: 577 496 1176
Fragments arranged by size:
1190 1176 767 758 729 599 577 540
496 112 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1637 1331
Size: 306 6704
BsiEI CG_ry'CG
Cuts at: 104 3267 4552 4598 5806 104
Size: 3163 1285 46 1208 1308
Fragments arranged by size:
3163 1308 1285 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1600 1824 2503 4024 4802 4992 5784 1600
Size: 224 679 1521 778 190 792 2826
Fragments arranged by size:
2826 1521 792 778 679 224 190
BsmI GAATG_Cn'
Cuts at: 3985 3985
Size: 7010
Bsp1286I G_dGCh'C
Cuts at: 1316 1600 1627 1800 1811 1824 2503 4024 4635
Size: 284 27 173 11 13 679 1521 611
Cuts at: 4635 4728 4802 4992 5054 5784 1316
Size: 93 74 190 62 730 2542
Fragments arranged by size:
2542 1521 730 679 611 284 190 173
93 74 62 27 13 11
BspEI T'CCGG_A
Cuts at: 6230 6230
Size: 7010
BspHI T'CATG_A
Cuts at: 6186 6186
Size: 7010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1503 1521 1528 1572 4060 4274 4576 4957 1503
Size: 18 7 44 2488 214 302 381 3556
Fragments arranged by size:
3556 2488 381 302 214 44 18 7
BsrBI CCG'CTC
Cuts at: 50 1794 5302 5356 6840 7005 50
Size: 1744 3508 54 1484 165 55
Fragments arranged by size:
3508 1744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2591 4922 2591
Size: 2331 4679
BsrFI r'CCGG_y
Cuts at: 1067 1441 2507 5008 5189 5398 1067
Size: 374 1066 2501 181 209 2679
Fragments arranged by size:
2679 2501 1066 374 209 181
BsrGI T'GTAC_A
Cuts at: 285 285
Size: 7010
BssSI C'ACGA_G
Cuts at: 5281 5639 5281
Size: 358 6652
BstAPI GCAn_nnn'nTGC
Cuts at: 4387 4387
Size: 7010
BstBI TT'CG_AA
Cuts at: 110 5371 110
Size: 5261 1749
BstEII G'GTnAC_C
Cuts at: 712 712
Size: 7010
BstXI CCAn_nnnn'nTGG
Cuts at: 796 2794 796
Size: 1998 5012
BstYI r'GATC_y
Cuts at: 1 660 1253 1286 1434 1615 2106 2976 4035
Size: 659 593 33 148 181 491 870 1059
Cuts at: 4035 4502 4525 4860 5106 6118 6204 6216 6562
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6562 1
Size: 449
Fragments arranged by size:
1059 1012 870 659 593 491 467 449
346 335 246 181 148 86 33 23
12
Bsu36I CC'TnA_GG
Cuts at: 3204 3204
Size: 7010
BtgI C'CryG_G
Cuts at: 1157 1779 1826 3642 1157
Size: 622 47 1816 4525
Fragments arranged by size:
4525 1816 622 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4960 5141 5304 4960
Size: 181 163 6666
Fragments arranged by size:
6666 181 163
BtsI GCAGTG_nn'
Cuts at: 215 395 1136 2772 3479 3942 4253 215
Size: 180 741 1636 707 463 311 2972
Fragments arranged by size:
2972 1636 741 707 463 311 180
ClaI AT'CG_AT
Cuts at: 257 257
Size: 7010
DraI TTT'AAA
Cuts at: 4009 4009
Size: 7010
DraIII CAC_nnn'GTG
Cuts at: 1211 1660 2017 1211
Size: 449 357 6204
Fragments arranged by size:
6204 449 357
DrdI GACnn_nn'nnGTC
Cuts at: 721 4716 5574 721
Size: 3995 858 2157
Fragments arranged by size:
3995 2157 858
EaeI y'GGCC_r
Cuts at: 1069 1176 4595 4769 5160 5187 6532 1069
Size: 107 3419 174 391 27 1345 1547
Fragments arranged by size:
3419 1547 1345 391 174 107 27
EagI C'GGCC_G
Cuts at: 4595 4595
Size: 7010
EarI CTCTTCn'nnn_
Cuts at: 179 640 742 1264 2089 2432 2816 3041 5033
Size: 461 102 522 825 343 384 225 1992
Cuts at: 5033 5243 179
Size: 210 1946
Fragments arranged by size:
1992 1946 825 522 461 384 343 225
210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1968 2907 5218 5528 5674 6820 1968
Size: 939 2311 310 146 1146 2158
Fragments arranged by size:
2311 2158 1146 939 310 146
EcoICRI GAG'CTC
Cuts at: 4022 4022
Size: 7010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 289 713 1330 1372 1745 1810 2110 2840
Size: 86 424 617 42 373 65 300 730
Cuts at: 2840 2856 3356 3566 4835 5267 6014 6282 203
Size: 16 500 210 1269 432 747 268 931
Fragments arranged by size:
1269 931 747 730 617 500 432 424
373 300 268 210 86 65 42 16
EcoO109I rG'GnC_Cy
Cuts at: 1411 1797 2290 2814 4149 1411
Size: 386 493 524 1335 4272
Fragments arranged by size:
4272 1335 524 493 386
EcoRI G'AATT_C
Cuts at: 734 4014 734
Size: 3280 3730
EcoRV GAT'ATC
Cuts at: 3522 3522
Size: 7010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 793 2007 5029 793
Size: 1214 3022 2774
Fragments arranged by size:
3022 2774 1214
FspI TGC'GCA
Cuts at: 4791 4791
Size: 7010
HaeII r_GCGC'y
Cuts at: 4444 4692 5714 6693 4444
Size: 248 1022 979 4761
Fragments arranged by size:
4761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 409 441 442 454 474 486 2384
Size: 32 13 32 1 12 20 12 1898
Cuts at: 2384 2416 2824 2856 3200 3232 4852 4882 4884
Size: 32 408 32 344 32 1620 30 2
Cuts at: 4884 4914 364
Size: 30 2460
Fragments arranged by size:
2460 1898 1620 408 344 32 32 32
32 32 30 30 20 13 12 12
2 1
HincII GTy'rAC
Cuts at: 360 639 1977 3510 4049 360
Size: 279 1338 1533 539 3321
Fragments arranged by size:
3321 1533 1338 539 279
HindIII A'AGCT_T
Cuts at: 1834 3763 1834
Size: 1929 5081
HpaI GTT'AAC
Cuts at: 1977 1977
Size: 7010
KasI G'GCGC_C
Cuts at: 4440 4688 4440
Size: 248 6762
KpnI G_GTAC'C
Cuts at: 4030 4030
Size: 7010
MluI A'CGCG_T
Cuts at: 6443 6443
Size: 7010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1028 3431 5681 5865 1028
Size: 2403 2250 184 2173
Fragments arranged by size:
2403 2250 2173 184
MscI TGG'CCA
Cuts at: 1178 4771 1178
Size: 3593 3417
MslI CAynn'nnrTG
Cuts at: 4999 5126 4999
Size: 127 6883
MspA1I CmG'CkG
Cuts at: 2579 2804 3505 4121 4435 4795 5808 6053 2579
Size: 225 701 616 314 360 1013 245 3536
Fragments arranged by size:
3536 1013 701 616 360 314 245 225
NaeI GCC'GGC
Cuts at: 1069 5191 1069
Size: 4122 2888
NarI GG'CG_CC
Cuts at: 4441 4689 4441
Size: 248 6762
NcoI C'CATG_G
Cuts at: 1157 1779 1826 3642 1157
Size: 622 47 1816 4525
Fragments arranged by size:
4525 1816 622 47
NdeI CA'TA_TG
Cuts at: 2112 2112
Size: 7010
NgoMIV G'CCGG_C
Cuts at: 1067 5189 1067
Size: 4122 2888
NheI G'CTAG_C
Cuts at: 306 306
Size: 7010
NsiI A_TGCA'T
Cuts at: 381 3374 381
Size: 2993 4017
NspI r_CATG'y
Cuts at: 732 1788 2499 4063 4563 5470 732
Size: 1056 711 1564 500 907 2272
Fragments arranged by size:
2272 1564 1056 907 711 500
PasI CC'CwG_GG
Cuts at: 3860 3860
Size: 7010
PciI A'CATG_T
Cuts at: 2495 5466 2495
Size: 2971 4039
PflMI CCAn_nnn'nTGG
Cuts at: 2980 2980
Size: 7010
PfoI T'CCnGG_A
Cuts at: 1262 2087 1262
Size: 825 6185
PmeI GTTT'AAAC
Cuts at: 4009 4009
Size: 7010
PmlI CAC'GTG
Cuts at: 1208 1208
Size: 7010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1303 1335 2926 2958 6149 6181 1303
Size: 32 1591 32 3191 32 2132
Fragments arranged by size:
3191 2132 1591 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2814 2814
Size: 7010
PspOMI G'GGCC_C
Cuts at: 1796 1796
Size: 7010
PstI C_TGCA'G
Cuts at: 1861 4057 4742 1861
Size: 2196 685 4129
Fragments arranged by size:
4129 2196 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7010
PvuII CAG'CTG
Cuts at: 4435 4795 4435
Size: 360 6650
RsrII CG'GwC_CG
Cuts at: 5205 5205
Size: 7010
SacI G_AGCT'C
Cuts at: 4024 4024
Size: 7010
SalI G'TCGA_C
Cuts at: 4047 4047
Size: 7010
SapI GCTCTTCn'nnn_
Cuts at: 640 5033 5243 640
Size: 4393 210 2407
Fragments arranged by size:
4393 2407 210
SbfI CC_TGCA'GG
Cuts at: 4057 4057
Size: 7010
ScaI AGT'ACT
Cuts at: 2683 6527 2683
Size: 3844 3166
SfcI C'TryA_G
Cuts at: 33 170 482 1484 1857 2244 2375 2539 3017
Size: 137 312 1002 373 387 131 164 478
Cuts at: 3017 3500 4053 4738 5731 5922 33
Size: 483 553 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 553 483 478 387
373 312 191 164 137 131
SfoI GGC'GCC
Cuts at: 4442 4690 4442
Size: 248 6762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7010
SmaI CCC'GGG
Cuts at: 2264 2920 4032 2264
Size: 656 1112 5242
Fragments arranged by size:
5242 1112 656
SmlI C'TyrA_G
Cuts at: 594 608 678 1168 1877 4165 5080 5572 5834
Size: 14 70 490 709 2288 915 492 262
Cuts at: 5834 594
Size: 1770
Fragments arranged by size:
2288 1770 915 709 492 490 262 70
14
SnaBI TAC'GTA
Cuts at: 4002 4002
Size: 7010
SpeI A'CTAG_T
Cuts at: 67 3364 67
Size: 3297 3713
SphI G_CATG'C
Cuts at: 732 1788 4063 4563 732
Size: 1056 2275 500 3179
Fragments arranged by size:
3179 2275 1056 500
StuI AGG'CCT
Cuts at: 3196 3196
Size: 7010
StyI C'CwwG_G
Cuts at: 1157 1779 1826 2957 3642 4144 4498 1157
Size: 622 47 1131 685 502 354 3669
Fragments arranged by size:
3669 1131 685 622 502 354 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 1381 2141 2315 2662 3069 3281 3852 4593
Size: 1250 760 174 347 407 212 571 741
Cuts at: 4593 4726 5185 5394 131
Size: 133 459 209 1747
Fragments arranged by size:
1747 1250 760 741 571 459 407 347
212 209 174 133
TatI w'GTAC_w
Cuts at: 285 1424 2423 2681 6525 285
Size: 1139 999 258 3844 770
Fragments arranged by size:
3844 1139 999 770 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1192 1224 2211 2243 6527 6559 1192
Size: 32 987 32 4284 32 1643
Fragments arranged by size:
4284 1643 987 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3429 4807 3429
Size: 1378 5632
XbaI T'CTAG_A
Cuts at: 4041 4041
Size: 7010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1155 3063 3727 4505 1155
Size: 1908 664 778 3660
Fragments arranged by size:
3660 1908 778 664
XhoI C'TCGA_G
Cuts at: 1877 1877
Size: 7010
XmaI C'CCGG_G
Cuts at: 2262 2918 4030 2262
Size: 656 1112 5242
Fragments arranged by size:
5242 1112 656
XmnI GAAnn'nnTTC
Cuts at: 418 1837 2189 3341 3829 418
Size: 1419 352 1152 488 3599
Fragments arranged by size:
3599 1419 1152 488 352
ZraI GAC'GTC
Cuts at: 3991 3991
Size: 7010
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AscI AvrII BaeI BcgI
BglI BsiWI BsmBI BssHII BstZ17I CspCI EcoNI FseI
FspAI MfeI NotI NruI PacI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI